{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:25:21Z","timestamp":1791213921045,"version":"4.1.0"},"reference-count":59,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[1999,8,1]],"date-time":"1999-08-01T00:00:00Z","timestamp":933465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Cell Research"],"published-print":{"date-parts":[[1999,8]]},"DOI":"10.1006\/excr.1999.4553","type":"journal-article","created":{"date-parts":[[2002,10,7]],"date-time":"2002-10-07T14:19:19Z","timestamp":1034000359000},"page":"185-193","source":"Crossref","is-referenced-by-count":22,"title":["Annexin V Binds to the Actin-Based Cytoskeleton at the Plasma Membrane of Activated Platelets"],"prefix":"10.1006","volume":"251","author":[{"given":"Eleni","family":"Tzima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Trotter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret A.","family":"Orchard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1006\/excr.1999.4553_RF1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0268-960X(95)90020-9","article-title":"Platelet activation","volume":"9","author":"Blockmans","year":"1995","journal-title":"Blood Rev."},{"key":"10.1006\/excr.1999.4553_RF2","series-title":"Thromb. Haemostasis.","article-title":"The platelet cytoskeleton","author":"Fox","year":"1987"},{"key":"10.1006\/excr.1999.4553_RF3","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1007\/978-1-4615-9442-0_15","article-title":"Hydrolysis of cytoskeletal proteins by the calcium-dependent protease during platelet activation","volume":"192","author":"Fox","year":"1985","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1006\/excr.1999.4553_RF4","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1083\/jcb.111.2.483","article-title":"Role of the membrane skeleton in preventing the shedding of procoagulant microvesicles from the platelet plasma membrane","volume":"111","author":"Fox","year":"1990","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF5","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1083\/jcb.120.6.1501","article-title":"Evidence that activation of platelet calpain is induced as a consequence of binding of adhesive ligand to the integrin, glycoprotein IIb\u2013IIIa","volume":"120","author":"Fox","year":"1993","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF6","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1042\/bj2850341","article-title":"Agonist-evoked Ca2+ entry in human platelets","volume":"285","author":"Sage","year":"1992","journal-title":"Biochem. J."},{"key":"10.1006\/excr.1999.4553_RF7","series-title":"Cellular Calcium\u2014A Practical Approach.","article-title":"Annexins: Calcium-dependent phospholipid-binding proteins","author":"Boustead","year":"1991"},{"key":"10.1006\/excr.1999.4553_RF8","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0304-4157(94)90019-1","article-title":"Annexins\u2014The problem of assessing the biological role for a gene family of multifunctional calcium and phospholipid binding proteins","volume":"1197","author":"Raynal","year":"1994","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/excr.1999.4553_RF9","doi-asserted-by":"crossref","first-page":"3392","DOI":"10.1016\/S0021-9258(19)39780-7","article-title":"Calcium-dependent regulation of actin filament bundling by lipocortin-85","volume":"265","author":"Ikebuchi","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF10","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1172\/JCI115892","article-title":"Human neutrophil annexin I promotes granule aggregation and modulates Ca2+-dependent membrane fusion","volume":"90","author":"Francis","year":"1992","journal-title":"J. Clin. Invest."},{"key":"10.1006\/excr.1999.4553_RF11","doi-asserted-by":"crossref","first-page":"6931","DOI":"10.1016\/S0021-9258(18)48333-0","article-title":"Characterization of Ca2+-dependent phospholipid binding and phosphorylation of lipocortin I","volume":"262","author":"Schlaepfer","year":"1987","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF12","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1105\/tpc.8.2.333","article-title":"Tomato annexins p34 and p35 bind to F-actin and display nucleotide phosphodiesterase activity inhibited by phospholipid binding","volume":"8","author":"Calvert","year":"1996","journal-title":"Plant Cell."},{"key":"10.1006\/excr.1999.4553_RF13","doi-asserted-by":"crossref","first-page":"17659","DOI":"10.1016\/S0021-9258(17)32491-2","article-title":"Annexin VI binding proteins in brain\u2014Interaction of annexin VI with a membrane skeletal protein, calspectin (brain spectrin or fodrin)","volume":"269","author":"Watanabe","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF14","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1111\/j.1432-1033.1996.0777w.x","article-title":"Characterisation of the interaction between annexin I and profilin","volume":"238","author":"Alvarez-Martinez","year":"1996","journal-title":"Eur. J. Biochem."},{"key":"10.1006\/excr.1999.4553_RF15","first-page":"174","article-title":"Placental anticoagulant protein-I: Measurement in extracellular fluids and cells of the hemostatic system","volume":"115","author":"Flaherty","year":"1990","journal-title":"J. Lab. Clin. Med."},{"key":"10.1006\/excr.1999.4553_RF16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1097\/00001721-199202000-00003","article-title":"Annexins I to VI: Quantitative determination in different human cell types and in plasma after myocardial infection","volume":"3","author":"Romisch","year":"1992","journal-title":"Blood Coag. Fibrinol."},{"key":"10.1006\/excr.1999.4553_RF17","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1016\/0006-291X(92)90279-T","article-title":"Evidence for the presence and location of annexins in human platelets","volume":"189","author":"Murphy","year":"1992","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1006\/excr.1999.4553_RF18","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1242\/jcs.108.5.2065","article-title":"The in vivo role of annexin VII (synexin): Characterization of an annexin VII-deficient Dictyostelium mutant indicates an involvement in Ca2+-regulated processes","volume":"108","author":"D\u00f6ring","year":"1995","journal-title":"J. Cell Sci."},{"key":"10.1006\/excr.1999.4553_RF19","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0167-4889(94)90161-9","article-title":"Thrombin stimulates the intracellular relocation of annexin V in human platelets","volume":"1222","author":"Trotter","year":"1994","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/excr.1999.4553_RF20","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1042\/bj3080591","article-title":"Calcium concentration during binding determines the manner in which annexin V binds to membranes","volume":"308","author":"Trotter","year":"1995","journal-title":"Biochem. J."},{"key":"10.1006\/excr.1999.4553_RF21","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1042\/bj3280447","article-title":"Relocation of annexin V to platelet membranes is a phosphorylation-dependent process","volume":"328","author":"Trotter","year":"1997","journal-title":"Biochem. J."},{"key":"10.1006\/excr.1999.4553_RF22","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1055\/s-0038-1651042","article-title":"Ouabain enhances basal and stimulus-induced cytoplasmic calcium concentrations in platelets","volume":"62","author":"Lees","year":"1989","journal-title":"Thromb. Haemostasis."},{"key":"10.1006\/excr.1999.4553_RF23","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1042\/bj1760899","article-title":"Calcium ions and the regulation of NAD+-linked isocitrate dehydrogenase from the mitochondria of rat heart and other tissues","volume":"176","author":"Denton","year":"1978","journal-title":"Biochem. J."},{"key":"10.1006\/excr.1999.4553_RF24","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1083\/jcb.108.2.467","article-title":"Association of gelsolin with actin filaments and cell membranes of macrophages and platelets","volume":"108","author":"Hartwig","year":"1989","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF25","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1006\/excr.1999.4553_RF26","first-page":"83","article-title":"Effect of calcium on the morphology of human platelets spread on glass substrates","volume":"30","author":"Zobel","year":"1983","journal-title":"Eur. J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF27","first-page":"86","article-title":"Organisation of the actin cytoskeleton of resting and activated platelets in suspension","volume":"123","author":"Escolar","year":"1986","journal-title":"Am. J. Pathol."},{"key":"10.1006\/excr.1999.4553_RF28","first-page":"45","article-title":"The cytoskeleton of blood platelets as viewed by immunofluorescence microscopy","volume":"24","author":"Debus","year":"1981","journal-title":"Eur. J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF29","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1161\/01.ATV.16.2.230","article-title":"Association of localised calcium gradients with redistribution of glycoproteins IIb\u2013IIa and F-actin in activated human blood platelets","volume":"16","author":"Ariyoshi","year":"1996","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"10.1006\/excr.1999.4553_RF30","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1247\/csf.13.89","article-title":"Lipocortin-like 33kDa protein of guinea pig neutrophil. Its distribution and stimulation-dependent translocation detected by monoclonal anti-33 kDa protein antibody","volume":"13","author":"Sato","year":"1988","journal-title":"Cell Struct. Funct."},{"key":"10.1006\/excr.1999.4553_RF31","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1242\/jcs.109.1.247","article-title":"Annexins II, IV, V and VI relocate in response to rises in intracellular calcium in human foreskin fibroblasts","volume":"109","author":"Barwise","year":"1996","journal-title":"J. Cell Sci."},{"key":"10.1006\/excr.1999.4553_RF32","doi-asserted-by":"crossref","first-page":"321","DOI":"10.3109\/09687689509072434","article-title":"Calcium-induced relocation of annexin IV and annexin V in the human osteosarcoma cell line MG-63","volume":"12","author":"Mohiti","year":"1995","journal-title":"Mol. Mem. Biol."},{"key":"10.1006\/excr.1999.4553_RF33","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1083\/jcb.98.2.473","article-title":"Changes in the distribution of the 34-kDa tyrosine kinase substrate during differentiation and maturation of chicken tissues","volume":"98","author":"Greenberg","year":"1984","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF34","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1365-2362.1995.tb01555.x","article-title":"Relationship of actin filament assembly to clearance of fibrinogen gold, GPIIb\u2013IIIa complexes on spread platelets","volume":"25","author":"White","year":"1995","journal-title":"Eur. J. Clin. Invest."},{"key":"10.1006\/excr.1999.4553_RF35","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1083\/jcb.98.4.1572","article-title":"Role of anchorin CII, a 31000-mol-wt membrane protein, in the interaction of chondrocytes with type II collagen","volume":"98","author":"Mollenhauer","year":"1984","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF36","doi-asserted-by":"crossref","first-page":"2335","DOI":"10.1002\/j.1460-2075.1988.tb03077.x","article-title":"Biosynthesis, secretion and extracellular localisation of anchorin CII, a collagen-binding protein of the calpactin family","volume":"7","author":"Pfaeffle","year":"1988","journal-title":"EMBO J."},{"key":"10.1006\/excr.1999.4553_RF37","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1182\/blood.V80.11.2774.2774","article-title":"Detergent-resistant cytoskeleton of the surface-activated platelet differs from the suspension-activated platelet cytoskeleton","volume":"80","author":"Smith","year":"1992","journal-title":"Blood"},{"key":"10.1006\/excr.1999.4553_RF38","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0014-5793(87)80038-8","article-title":"Neutrophil specific 33kDa protein: Its Ca2+- and phospholipid-dependent intracellular translocation","volume":"214","author":"Sato","year":"1987","journal-title":"FEBS Lett."},{"key":"10.1006\/excr.1999.4553_RF39","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0014-5793(89)81097-X","article-title":"Expression of the cDNA encoding lipocortin-like 39kDa protein of guinea pig neutrophils in yeast. Purification and biological characterization","volume":"255","author":"Sato","year":"1989","journal-title":"FEBS Lett."},{"key":"10.1006\/excr.1999.4553_RF40","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1128\/IAI.62.1.126-134.1994","article-title":"Selective translocation of annexins during intracellular redistribution of chlamydia trachomatis in HeLa and McCoy cells","volume":"62","author":"Majeed","year":"1994","journal-title":"Infect. Immun."},{"key":"10.1006\/excr.1999.4553_RF41","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1091\/mbc.8.3.533","article-title":"Specific release of membrane-bound annexin II and cortical cytoskeletal elements by sequestration of membrane cholesterol","volume":"8","author":"Harder","year":"1997","journal-title":"Mol. Biol. Cell."},{"key":"10.1006\/excr.1999.4553_RF42","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/0014-4827(91)90259-W","article-title":"Actin-binding proteins involved in the capping of epidermal growth factor receptors in A431 cells","volume":"196","author":"Kwiatkowska","year":"1991","journal-title":"Exp. Cell Res."},{"key":"10.1006\/excr.1999.4553_RF43","doi-asserted-by":"crossref","first-page":"6078","DOI":"10.1073\/pnas.84.17.6078","article-title":"Structural and functional characterization of endonexin II, a calcium- and phospholipid-binding protein","volume":"84","author":"Schlaepfer","year":"1987","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/excr.1999.4553_RF44","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/S0008-6363(95)00054-2","article-title":"Annexin V is localized in association with Z-line of rat cardiac myocytes","volume":"30","author":"Wang","year":"1995","journal-title":"Cardiovasc. Res."},{"key":"10.1006\/excr.1999.4553_RF45","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1146\/annurev.cb.10.110194.001231","article-title":"Structure of actin binding proteins: Insights about function at atomic resolution","volume":"10","author":"Pollard","year":"1994","journal-title":"Ann. Rev. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF46","doi-asserted-by":"crossref","first-page":"5417","DOI":"10.1073\/pnas.80.17.5417","article-title":"Human platelets contain phospholipase C that hydrolyses polyphosphoinositides","volume":"80","author":"Rittenhouse","year":"1983","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/excr.1999.4553_RF47","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/312315a0","article-title":"Inositol triphosphate, a novel second messenger in cellular signal transduction","volume":"312","author":"Berridge","year":"1984","journal-title":"Nature"},{"key":"10.1006\/excr.1999.4553_RF48","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1083\/jcb.106.5.1525","article-title":"Identification of a membrane skeleton in platelets","volume":"106","author":"Fox","year":"1988","journal-title":"J. Cell Biol."},{"key":"10.1006\/excr.1999.4553_RF49","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/0014-5793(95)00320-9","article-title":"The association of pp125FAK, pp60src, CDC42Hs and Rap1B with the cytoskeleton of aggegated platelets is a reversible process regulated by calcium","volume":"363","author":"Dash","year":"1995","journal-title":"FEBS Lett."},{"key":"10.1006\/excr.1999.4553_RF50","doi-asserted-by":"crossref","first-page":"25973","DOI":"10.1016\/S0021-9258(19)74482-2","article-title":"On the role of the platelet membrane skeleton in mediating signal transduction\u2014Association of GPIIb\u2013IIIa, pp60(c-src), pp62(c-yes), and the p21(ras)GTPase-activating protein with the membrane skeleton","volume":"268","author":"Fox","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF51","first-page":"102","article-title":"Platelet activation: New aspects","volume":"26","author":"Fox","year":"1996","journal-title":"Haematology"},{"key":"10.1006\/excr.1999.4553_RF52","doi-asserted-by":"crossref","first-page":"7004","DOI":"10.1074\/jbc.271.12.7004","article-title":"The platelet cytoskeleton stabilises the interaction between alpha(IIb)beta(3) and its ligand and induces selective movements of ligand-occupied integrin","volume":"271","author":"Fox","year":"1996","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF53","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1242\/jeb.139.1.253","article-title":"The cytoskeleton as a barrier to exocytosis in secretory cells","volume":"139","author":"Aunis","year":"1988","journal-title":"J. Exp. Biol."},{"key":"10.1006\/excr.1999.4553_RF54","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1021\/bi00121a043","article-title":"Inhibition of protein kinase C by annexin V","volume":"31","author":"Schlaepfer","year":"1992","journal-title":"Biochemistry"},{"key":"10.1006\/excr.1999.4553_RF55","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1042\/bj3301277","article-title":"Annexin V inhibits protein kinase C activity via a mechanism of phospholipid sequestration","volume":"330","author":"Dubois","year":"1998","journal-title":"Biochem. J."},{"key":"10.1006\/excr.1999.4553_RF56","doi-asserted-by":"crossref","first-page":"10474","DOI":"10.1074\/jbc.272.16.10474","article-title":"Inhibition of cytosolic phospholipase A2 by annexin V in differentiated permeabilized HL-60 cells. Evidence of crucial importance of domain I type II Ca2+-binding site in the mechanism of inhibition","volume":"272","author":"Mira","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF57","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1093\/oxfordjournals.jbchem.a124000","article-title":"Evidence for an increase in the association of cytosolic phospholipase A2 with the cytoskeleton of stimulated rabbit platelets","volume":"113","author":"Akiba","year":"1993","journal-title":"J. Biochem. (Tokyo)"},{"key":"10.1006\/excr.1999.4553_RF58","doi-asserted-by":"crossref","first-page":"5400","DOI":"10.1016\/S0021-9258(18)83559-1","article-title":"Possible involvement of cytoskeleton in collagen-stimulated activation of phospholipases in human platelets","volume":"264","author":"Nakano","year":"1989","journal-title":"J. Biol. Chem."},{"key":"10.1006\/excr.1999.4553_RF59","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0898-6568(97)00121-6","article-title":"Protein kinase C and the cytoskeleton","volume":"10","author":"Keenan","year":"1998","journal-title":"Cell Signal"}],"container-title":["Experimental Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014482799945536?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014482799945536?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T08:20:32Z","timestamp":1620030032000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014482799945536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,8]]},"references-count":59,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,8]]}},"alternative-id":["S0014482799945536"],"URL":"https:\/\/doi.org\/10.1006\/excr.1999.4553","relation":{},"ISSN":["0014-4827"],"issn-type":[{"value":"0014-4827","type":"print"}],"subject":[],"published":{"date-parts":[[1999,8]]}}}