{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:35:10Z","timestamp":1790019310124,"version":"4.0.1"},"reference-count":28,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Parasitology"],"published-print":{"date-parts":[[2000,1]]},"DOI":"10.1006\/expr.1999.4458","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T21:57:25Z","timestamp":1033941445000},"page":"15-22","source":"Crossref","is-referenced-by-count":18,"title":["Leishmania donovani: Development and Characterisation of a Kinetoplast DNA Probe and Its Use in the Detection of Parasites"],"prefix":"10.1006","volume":"94","author":[{"given":"Bronwen","family":"Lambson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audra","family":"Smyth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas C.","family":"Barker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1006\/expr.1999.4458_RF1","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/0035-9203(95)90416-6","article-title":"Detection of Leishmania in the blood of early kala-azar patients with the aid of the polymerase chain reaction","volume":"89","author":"Adhya","year":"1995","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0035-9203(96)90112-1","article-title":"Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major: A comparison with direct microscopy of smears and sections from lesions","volume":"90","author":"Andresen","year":"1996","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF3","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4269\/ajtmh.1995.53.251","article-title":"Comparison of the polymerase chain reaction and serology for the detection of canine visceral leishmaniasis","volume":"53","author":"Ashford","year":"1995","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF4","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0035-9203(83)90146-3","article-title":"The use of DNA probes in the identification of leishmaniasis: Discrimination between isolates of the Leishmania mexicana and Leishmania braziliensis complexes","volume":"77","author":"Barker","year":"1983","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF5","doi-asserted-by":"crossref","first-page":"S139","DOI":"10.1017\/S0031182000085747","article-title":"The potential of using recombinant DNA species specific probes for the identification of tropical Leishmania","volume":"92","author":"Barker","year":"1986","journal-title":"Parasitology"},{"key":"10.1006\/expr.1999.4458_RF6","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4269\/ajtmh.1996.55.273","article-title":"Canine leishmaniasis: Identification of asymptomatic carriers by polymerase chain reaction and immunoblotting","volume":"55","author":"Berrahal","year":"1996","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF7","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0035-9203(95)90569-3","article-title":"Characterization of \u2018old world\u2019 Leishmania species using amplified minicircle variable regions as molecular probes","volume":"89","author":"Bozza","year":"1995","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF8","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1017\/S0031182000073650","article-title":"A kinetoplast DNA probe diagnostic for Leishmania infantum","volume":"105","author":"Gramiccia","year":"1992","journal-title":"Parasitology"},{"key":"10.1006\/expr.1999.4458_RF9","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1006\/expr.1995.1133","article-title":"Leishmania infantum: Sequence homology within minicircle classes regardless of geographical distance","volume":"81","author":"Guti\u00e9rrez-Solar","year":"1995","journal-title":"Experimental Parasitology"},{"key":"10.1006\/expr.1999.4458_RF10","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1128\/JCM.36.7.1989-1995.1998","article-title":"Single-step multiplex PCR assay for characterization of new world Leishmania complexes","volume":"36","author":"Harris","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1006\/expr.1999.4458_RF11","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1017\/S0031182000068086","article-title":"Enzymatic amplification of miniexon-derived RNA gene spacers of Leishmania donovani: Primers and probes for DNA diagnosis","volume":"107","author":"Hassan","year":"1993","journal-title":"Parasitology"},{"key":"10.1006\/expr.1999.4458_RF12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0166-6851(91)90221-Q","article-title":"A sensitive repetitive DNA probe that is specific to the Leishmania donovani complex and its use as an epidemiological and diagnostic reagent","volume":"44","author":"Howard","year":"1991","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1006\/expr.1999.4458_RF13","article-title":"Molecular and immuno-epidemiology of Leishmania donovani infections in an area of high transmission","author":"Ibrahim","year":"1999","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF14","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0166-6851(84)90088-4","article-title":"Novel identification of differences in the kinetoplast DNA of Leishmania isolates by recombinant DNA techniques and in situ hybridisation","volume":"12","author":"Kennedy","year":"1984","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1006\/expr.1999.4458_RF15","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0166-6851(91)90014-W","article-title":"Generation of species-specific probes for Leishmania aethiopica","volume":"44","author":"Laskay","year":"1991","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1006\/expr.1999.4458_RF16","doi-asserted-by":"crossref","first-page":"257","DOI":"10.4269\/ajtmh.1985.34.257","article-title":"Identification of pathogenic Leishmania promastigotes by DNA\u2013DNA hybridisation with kinetoplast DNA cloned into Escherichia-coli plasmids","volume":"34","author":"Lawrie","year":"1985","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF17","doi-asserted-by":"crossref","first-page":"308","DOI":"10.4269\/ajtmh.1988.38.308","article-title":"The use of nonradio-active DNA probes for the characterization of Leishmania isolates from Peru","volume":"38","author":"Lopez","year":"1988","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF18","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1128\/JCM.36.10.2877-2881.1998","article-title":"A nested-PCR-based schizodeme method for identifying Leishmania kinetoplast minicircle classes directly from clinical samples and its application to the study of the epidemiology of Leishmania tropica in Pakistan","volume":"36","author":"Noyes","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1006\/expr.1999.4458_RF19","doi-asserted-by":"crossref","first-page":"364","DOI":"10.4269\/ajtmh.1993.49.364","article-title":"Isolation and characterization of a repetitive DNA sequence from Leishmania infantum: Development of a visceral leishmaniasis polymerase chain reaction","volume":"49","author":"Piarroux","year":"1993","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF20","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1128\/MCB.9.3.1365","article-title":"Conserved sequence blocks in kinetoplast minicircles from diverse species of Trypanosomes","volume":"9","author":"Ray","year":"1989","journal-title":"Molecular and Cellular Biology"},{"key":"10.1006\/expr.1999.4458_RF21","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/0014-4894(90)90031-7","article-title":"Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania","volume":"71","author":"Rodgers","year":"1990","journal-title":"Experimental Parasitology"},{"key":"10.1006\/expr.1999.4458_RF22","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1073\/pnas.84.2.565","article-title":"Kinetoplast DNA minicircles: Regions of extensive sequence divergence","volume":"84","author":"Rogers","year":"1987","journal-title":"Proceedings of the National Academy of Sciences USA"},{"key":"10.1006\/expr.1999.4458_RF23","series-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"10.1006\/expr.1999.4458_RF24","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/0035-9203(95)90081-0","article-title":"Visceral leishmaniasis: Use of the polymerase chain reaction in an epidemiological study in Baringo District, Kenya","volume":"89","author":"Schaefer","year":"1995","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF25","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/S0035-9203(98)91042-2","article-title":"First identification of a species of Leishmania causing visceral leishmaniasis in the Sultanate of Oman, in a patient with AIDS","volume":"92","author":"Scrimgeour","year":"1998","journal-title":"Transactions of the Royal Society of Tropical Medicine and Hygiene"},{"key":"10.1006\/expr.1999.4458_RF26","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/0166-6851(89)90153-9","article-title":"A kinetoplast DNA probe diagnostic for Leishmania major: Sequence homologies between regions of Leishmania minicircles","volume":"37","author":"Smith","year":"1989","journal-title":"Molecular and Biochemical Parasitology"},{"key":"10.1006\/expr.1999.4458_RF27","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1017\/S0031182000074096","article-title":"Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kala-azar patients","volume":"105","author":"Smyth","year":"1992","journal-title":"Parasitology"},{"key":"10.1006\/expr.1999.4458_RF28","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0166-6851(92)90208-2","article-title":"Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites","volume":"51","author":"Van Eys","year":"1992","journal-title":"Molecular and Biochemical Parasitology"}],"container-title":["Experimental Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489499944582?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0014489499944582?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T07:50:55Z","timestamp":1620028255000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0014489499944582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["S0014489499944582"],"URL":"https:\/\/doi.org\/10.1006\/expr.1999.4458","relation":{},"ISSN":["0014-4894"],"issn-type":[{"value":"0014-4894","type":"print"}],"subject":[],"published":{"date-parts":[[2000,1]]}}}