{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T07:21:40Z","timestamp":1793258500213,"version":"4.5.0"},"reference-count":44,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[1999,12,1]],"date-time":"1999-12-01T00:00:00Z","timestamp":944006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Molecular Biology"],"published-print":{"date-parts":[[1999,12]]},"DOI":"10.1006\/jmbi.1999.3279","type":"journal-article","created":{"date-parts":[[2002,9,18]],"date-time":"2002-09-18T19:24:54Z","timestamp":1032377094000},"page":"757-770","source":"Crossref","is-referenced-by-count":59,"title":["Characterisation of the calcium-binding C-terminal domain of Clostridium perfringens alpha-toxin 1 1Edited by A Klug"],"prefix":"10.1006","volume":"294","author":[{"given":"Claire E","family":"Naylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Jepson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis T","family":"Crane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W","family":"Titball","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajit K","family":"Basak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Bolgiano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1006\/jmbi.1999.3279_BIB1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0014-5793(96)01279-3","article-title":"Efforts toward deriving the CD spectrum of a 310 helix in aqueous solution","volume":"399","author":"Andersen","year":"1996","journal-title":"FEBS Letters"},{"key":"10.1006\/jmbi.1999.3279_BIB2","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1365-2958.1995.tb02234.x","article-title":"Virulence studies on chromosomal \u03b1-toxin and \u0398-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of \u03b1-toxin in Clostridium perfringens-mediated gas gangrene","volume":"15","author":"Awad","year":"1995","journal-title":"Mol. Microbiol."},{"key":"10.1006\/jmbi.1999.3279_BIB3","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1006\/jmbi.1994.1758","article-title":"Purification, crystallization and preliminary X-ray diffraction studies of alpha-toxin of Clostridium perfringens","volume":"244","author":"Basak","year":"1994","journal-title":"J. Mol. Biol."},{"key":"10.1006\/jmbi.1999.3279_BIB4","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1107\/S0907444998005186","article-title":"Crystallisation and preliminary X-ray diffraction studies of \u03b1-toxin from two different strains (NCTC-8237 and CER89L43) of Clostridium perfringens","volume":"54","author":"Basak","year":"1998","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1006\/jmbi.1999.3279_BIB5","series-title":"Physical Methods to Characterize Pharmaceutical Proteins","first-page":"65","article-title":"Structural information on proteins from circular dichroism spectroscopy","author":"Bloemendal","year":"1995"},{"key":"10.1006\/jmbi.1999.3279_BIB6","series-title":"X-PLOR Manual, Version 3.1. A System for X-ray Crystallography and NMR","author":"Br\u00fcnger","year":"1992"},{"key":"10.1006\/jmbi.1999.3279_BIB7","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1128\/IAI.64.1.358-362.1996","volume":"64","author":"Bryant","year":"1996","journal-title":"Infect. Immun."},{"key":"10.1006\/jmbi.1999.3279_BIB8","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite","volume":"50","year":"1994","journal-title":"Acta Crystallog. sect. D"},{"key":"10.1006\/jmbi.1999.3279_BIB9","first-page":"946","article-title":"\u2018DM\u2019","volume":"34","author":"Cowtan","year":"1991","journal-title":"Joint CCP4 and ESRF-EACBM Newsletter Protein Crystallog."},{"key":"10.1006\/jmbi.1999.3279_BIB10","doi-asserted-by":"crossref","first-page":"2753","DOI":"10.1021\/bi962466t","article-title":"A ternary metal binding site in the C2 domain of phosphoinositide-specific phospholipase C-\u03b41","volume":"36","author":"Essen","year":"1997","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB11","series-title":"Proceedings of CCP4 Study Weekend, 1993, on Data Collection and Processing","first-page":"114","article-title":"Data reduction","author":"Evans","year":"1993"},{"key":"10.1006\/jmbi.1999.3279_BIB12","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1111\/j.1476-5381.1989.tb11931.x","article-title":"Contraction of the rat isolated aorta caused by Clostridium perfringens alpha-toxin (phospholipase C); evidence for the involvement of arachidonic acid metabolism","volume":"97","author":"Fujii","year":"1989","journal-title":"Brit. J. Pharmacol."},{"key":"10.1006\/jmbi.1999.3279_BIB13","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1107\/S0021889896004190","article-title":"Glycerol concentration required for cryoprotection of 50 typical protein crystallization solutions","volume":"29","author":"Garmen","year":"1997","journal-title":"Cryocrystallography Papers, J. Appl. Crystallog."},{"key":"10.1006\/jmbi.1999.3279_BIB14","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1038\/nsb0996-788","article-title":"C2 domain conformational changes in phospholipase C-\u03b41","volume":"3","author":"Grobler","year":"1996","journal-title":"Nature Struct. Biol."},{"key":"10.1006\/jmbi.1999.3279_BIB15","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1046\/j.1365-2958.1997.6161993.x","article-title":"The carboxyl-terminal C2-like domain of the \u03b1-toxin from Clostridium perfringens mediates calcium-dependent membrane recognition","volume":"26","author":"Guillouard","year":"1997","journal-title":"Mol. Microbiol."},{"key":"10.1006\/jmbi.1999.3279_BIB16","doi-asserted-by":"crossref","first-page":"363","DOI":"10.3109\/00365529009095500","article-title":"Phospholipase C from Clostridium perfringens stimulates phospholipase A2-mediated arachidonic acid release in cultured intestinal epithelial cells (INT 407)","volume":"25","author":"Gustafson","year":"1990","journal-title":"Scand. J. Gastroenterol."},{"key":"10.1006\/jmbi.1999.3279_BIB17","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1128\/CMR.3.1.66","article-title":"Toxigenic clostridia","volume":"3","author":"Hatheway","year":"1990","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1006\/jmbi.1999.3279_BIB18","series-title":"Metals and Micro-organisms","author":"Hughes","year":"1989"},{"key":"10.1006\/jmbi.1999.3279_BIB19","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/335651a0","article-title":"The role of protein surface charges in ion binding","volume":"355","author":"Linse","year":"1988","journal-title":"Nature"},{"key":"10.1006\/jmbi.1999.3279_BIB20","doi-asserted-by":"crossref","first-page":"8050","DOI":"10.1016\/S0021-9258(18)92938-8","article-title":"Calcium binding to calmodulin and its globular domains","volume":"266","author":"Linse","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1006\/jmbi.1999.3279_BIB21","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/0003-2697(92)90279-G","article-title":"Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins","volume":"200","author":"Mach","year":"1992","journal-title":"Anal. Biochem."},{"key":"10.1006\/jmbi.1999.3279_BIB22","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(87)90135-7","article-title":"Variable selection method improves the prediction of protein secondary structure from circular dichroism spectra","volume":"167","author":"Manavalan","year":"1987","journal-title":"Anal. Biochem."},{"key":"10.1006\/jmbi.1999.3279_BIB23","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.1021\/bi952522a","article-title":"Spectroscopic characterization of a high-affinity calmodulin-target peptide hybrid molecule","volume":"35","author":"Martin","year":"1996","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB24","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1099\/00221287-1-3-385","article-title":"The lecithinase of Clostridium bifermentans and its relation to the \u03b1-toxin of Clostridium welchii","volume":"1","author":"Miles","year":"1947","journal-title":"J. Gen. Microbiol."},{"key":"10.1006\/jmbi.1999.3279_BIB25","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1021\/bi00407a012","article-title":"A new kinetic approach for studying phospholipase C (Clostridium perfringens \u03b1-toxin) activity on phospholipid monolayers","volume":"27","author":"Moreau","year":"1988","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB26","doi-asserted-by":"crossref","first-page":"12011","DOI":"10.1021\/bi9717340","article-title":"Ca2+-signalling cycle of a membrane-docking C2 domain","volume":"36","author":"Nalefski","year":"1997","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB27","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/1447","article-title":"Structure of the key toxin in gas gangrene","volume":"5","author":"Naylor","year":"1998","journal-title":"Nature Struct. Biol."},{"key":"10.1006\/jmbi.1999.3279_BIB28","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/S0076-6879(97)76066-X","article-title":"Processing of X-ray diffraction data collected in oscillation mode","volume":"276","author":"Otwinowski","year":"1997","journal-title":"Methods Enzymol."},{"key":"10.1006\/jmbi.1999.3279_BIB29","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1074\/jbc.273.3.1596","article-title":"Crystal structure of a calcium-phospholipid binding domain from cytosolic phospholipase A2","volume":"273","author":"Perisic","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1006\/jmbi.1999.3279_BIB30","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1002\/pro.5560061002","article-title":"Calcium binding to tandem repeats of EGF-like modules","volume":"6","author":"Rand","year":"1997","journal-title":"Protein Sci."},{"key":"10.1006\/jmbi.1999.3279_BIB31","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1107\/S0108767386099622","article-title":"Improved Fourier coefficients for maps using phases from partial structures with errors","volume":"42","author":"Read","year":"1986","journal-title":"Acta Crystallog. Sect. A"},{"key":"10.1006\/jmbi.1999.3279_BIB32","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1128\/IAI.20.2.325-333.1978","article-title":"Effect of zinc and calcium ions on the production of alpha-toxin and proteases by Clostridium perfringens","volume":"20","author":"Sato","year":"1978","journal-title":"Infect. Immun."},{"key":"10.1006\/jmbi.1999.3279_BIB33","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1126\/science.273.5272.248","article-title":"Bipartite Ca2+-binding motif in C2 domains of synaptotagmin and protein kinase C","volume":"273","author":"Shao","year":"1996","journal-title":"Science"},{"key":"10.1006\/jmbi.1999.3279_BIB34","doi-asserted-by":"crossref","first-page":"16106","DOI":"10.1021\/bi981789h","article-title":"Solution structure of the Ca2+-free and Ca2+-bound C2A domain of synaptotagmin I","volume":"37","author":"Shao","year":"1998","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB35","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1093\/infdis\/157.2.272","article-title":"Lethal effects and cardiovascular effects of purified alpha- and theta-toxins from Clostridium perfringens","volume":"157","author":"Stevens","year":"1988","journal-title":"J. Infect. Dis."},{"key":"10.1006\/jmbi.1999.3279_BIB36","doi-asserted-by":"crossref","first-page":"113","DOI":"10.3109\/10409237409105445","article-title":"Aromatic contributions to circular dichroism of proteins","volume":"1","author":"Strickland","year":"1974","journal-title":"CRC Crit. Rev. Biochem."},{"key":"10.1006\/jmbi.1999.3279_BIB37","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1016\/S0969-2126(98)00139-7","article-title":"Structure of the protein kinase C\u03b2 phospholipid-binding C2 domain complexed with Ca2+","volume":"6","author":"Sutton","year":"1998","journal-title":"Structure"},{"key":"10.1006\/jmbi.1999.3279_BIB38","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/0092-8674(95)90296-1","article-title":"Structure of the first C2 domain of synaptotagmin I","volume":"80","author":"Sutton","year":"1995","journal-title":"Cell"},{"key":"10.1006\/jmbi.1999.3279_BIB39","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1128\/MR.57.2.347-366.1993","article-title":"Bacterial phospholipases C","volume":"57","author":"Titball","year":"1993","journal-title":"Microbiol. Rev."},{"key":"10.1006\/jmbi.1999.3279_BIB40","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1128\/IAI.59.5.1872-1874.1991","article-title":"Hemolytic and sphingomyelinase activities of Clostridium perfringens alpha-toxin are dependent on a domain homologous to that of an enzyme from the arachidonic acid pathway","volume":"59","author":"Titball","year":"1991","journal-title":"Infect. Immun."},{"key":"10.1006\/jmbi.1999.3279_BIB41","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1574-6968.1993.tb06293.x","article-title":"Biochemical and immunological properties of the C-terminal domain of the alpha-toxin of Clostridium perfringens","volume":"110","author":"Titball","year":"1993","journal-title":"FEMS Microbiol. Letters"},{"key":"10.1006\/jmbi.1999.3279_BIB42","doi-asserted-by":"crossref","first-page":"2396","DOI":"10.1021\/bi00552a018","article-title":"New calcium indicators and buffers with high selectivity against magnesium and protons","volume":"19","author":"Tsein","year":"1980","journal-title":"Biochemistry"},{"key":"10.1006\/jmbi.1999.3279_BIB43","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1016\/0264-410X(93)90051-X","article-title":"A genetically engineered vaccine against the alpha-toxin of Clostridium perfringens also protects mice against experimental gas gangrene","volume":"11","author":"Williamson","year":"1993","journal-title":"Vaccine"},{"key":"10.1006\/jmbi.1999.3279_BIB44","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1042\/bj2800407","article-title":"Kinetic mechanism of Clostridium perfringens phospholipase C","volume":"280","author":"Young","year":"1991","journal-title":"Biochem. J."}],"container-title":["Journal of Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283699932795?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0022283699932795?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T02:40:49Z","timestamp":1620009649000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0022283699932795"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,12]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,12]]}},"alternative-id":["S0022283699932795"],"URL":"https:\/\/doi.org\/10.1006\/jmbi.1999.3279","relation":{},"ISSN":["0022-2836"],"issn-type":[{"value":"0022-2836","type":"print"}],"subject":[],"published":{"date-parts":[[1999,12]]}}}