{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T10:17:11Z","timestamp":1758709031472},"reference-count":51,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[1999,6,1]],"date-time":"1999-06-01T00:00:00Z","timestamp":928195200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicology and Applied Pharmacology"],"published-print":{"date-parts":[[1999,6]]},"DOI":"10.1006\/taap.1999.8669","type":"journal-article","created":{"date-parts":[[2002,9,18]],"date-time":"2002-09-18T22:01:25Z","timestamp":1032386485000},"page":"157-165","source":"Crossref","is-referenced-by-count":7,"title":["Immunochemical Analysis of Liver Microsomal Cytochromes P450 of the American Alligator, Alligator mississippiensis"],"prefix":"10.1006","volume":"157","author":[{"given":"Robin P.","family":"Ertl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stelvio M.","family":"Bandiera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald R.","family":"Buhler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Stegeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary W.","family":"Winston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/taap.1999.8669_RF1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/0003-9861(92)90574-G","article-title":"Effects of testosterone and estrogen on hepatic levels of cytochromes P450 2C7 and P450 2C11 in the rat","volume":"296","author":"Bandiera","year":"1992","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/taap.1999.8669_RF2","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1016\/0006-2952(95)98511-7","article-title":"Catalytic and immunologic characterization of hepatic and lung cytochromes P450 in the polar bear","volume":"49","author":"Bandiera","year":"1995","journal-title":"Biochem. Pharmacol."},{"key":"10.1006\/taap.1999.8669_RF3","first-page":"11","article-title":"Aquaculture drug development: A pharmaceutical industry perspective","volume":"33","author":"Beleau","year":"1991","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF4","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0003-9861(73)80023-2","article-title":"Fluorometric assay of proteins in the nanogram range","volume":"155","author":"B\u00f6hlen","year":"1973","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/taap.1999.8669_RF5","first-page":"1419","article-title":"Sebekiosis in captive alligator hatchlings","volume":"185","author":"Boyce","year":"1984","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"10.1006\/taap.1999.8669_RF6","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1006\/abbi.1994.1278","article-title":"Cloning and sequencing of the major rainbow trout constitutive cytochrome P450 (CYP2K1): Identification of a new cytochrome P450 gene subfamily and its expression in mature rainbow trout liver and trunk kidney","volume":"312","author":"Buhler","year":"1994","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/taap.1999.8669_RF7","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0009-2797(83)90072-8","article-title":"Differential effects of phenobarbitone and 3-methylcholanthrene induction on the hepatic microsomal metabolism and cytochrome P-450-binding of phenoxazone and a homologous series of its n-alkyl ethers (alkoxyresorufins)","volume":"45","author":"Burke","year":"1983","journal-title":"Chem.\u2013Biol. Interact."},{"key":"10.1006\/taap.1999.8669_RF8","first-page":"1","article-title":"Husbandry and preventative medicine practices that increase reproductive efficiency of breeding colonies of alligators","author":"Cardeilhac","year":"1988","journal-title":"Aquaculture Report Series"},{"key":"10.1006\/taap.1999.8669_RF9","first-page":"21","article-title":"Preliminary studies on the use of oxytetracycline to control hatchling alligator syndrome (HAS)","volume":"1","author":"Cardeilhac","year":"1986","journal-title":"Proc. Int. Assoc. Aquat. Anim. Med."},{"key":"10.1006\/taap.1999.8669_RF10","first-page":"157","article-title":"Methods used to evaluate drugs for alligator production","volume":"19","author":"Cardeilhac","year":"1988","journal-title":"IAAAM"},{"key":"10.1006\/taap.1999.8669_RF11","series-title":"Biomarker Research and Application in the Assessment of Environmental Health, NATO ASI Series","first-page":"49","article-title":"Interpretation of biomarker responses","author":"Di Giulio","year":"1993"},{"key":"10.1006\/taap.1999.8669_RF12","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/(SICI)1099-0461(1999)13:1<17::AID-JBT3>3.0.CO;2-6","article-title":"Liver microsomal cytochromes P450-dependent alkoxyphenoxazone O-dealkylation in vitro by alligator and rat: Activities, inhibition, substrate preference and discrimination factors","volume":"13","author":"Ertl","year":"1998","journal-title":"J. Biochem. Mol. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF13","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1016\/S0006-2952(98)00003-3","article-title":"Induction time course of cytochromes P450 by phenobarbital- and 3-methylcholanthrene-pretreatment in liver microsomes of Alligator mississippiensis","volume":"55","author":"Ertl","year":"1998","journal-title":"Biochem. Pharmacol."},{"key":"10.1006\/taap.1999.8669_RF14","first-page":"85","article-title":"The microsomal mixed function oxidase system of amphibians and reptiles: Components, activities and induction","volume":"121","author":"Ertl","year":"1998","journal-title":"Comp. Biochem. Physiol. Part C"},{"key":"10.1006\/taap.1999.8669_RF15","first-page":"19612","volume":"51","year":"1986","journal-title":"Fed. Reg."},{"key":"10.1006\/taap.1999.8669_RF16","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1042\/bj2030161","article-title":"Cytochrome P-450 induction by clofibrate","volume":"203","author":"Gibson","year":"1982","journal-title":"Biochem. J."},{"key":"10.1006\/taap.1999.8669_RF17","first-page":"2","article-title":"IR-4\/FDA workshop for minor use drugs: Focus on aquaculture. Opening remarks","volume":"33","author":"Guest","year":"1991","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF18","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1289\/ehp.95103s7157","article-title":"Organization versus activation: The role of endocrine-disrupting contaminants (EDCs) during embryonic development in wildlife","volume":"103","author":"Guillette","year":"1995","journal-title":"Environ. Health Perspect."},{"key":"10.1006\/taap.1999.8669_RF19","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1289\/ehp.94102680","article-title":"Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida","volume":"102","author":"Guillette","year":"1994","journal-title":"Environ. Health Perspect."},{"key":"10.1006\/taap.1999.8669_RF20","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1006\/gcen.1996.0005","article-title":"Reduction in penis size and plasma testosterone concentrations in juvenile alligators living in contaminated environments","volume":"101","author":"Guillette","year":"1996","journal-title":"Gen. Comp. Endocrinol."},{"key":"10.1006\/taap.1999.8669_RF21","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/0041-008X(89)90240-8","article-title":"Cloned rainbow trout liver P(1)450 complementary DNA as a potential environmental monitor","volume":"98","author":"Haasch","year":"1989","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"10.1006\/taap.1999.8669_RF22","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.3109\/00498258909043171","article-title":"The hepatic microsomal mixed-function oxygenase (MFO) system of Alligator mississippiensis: Induction by 3-methylcholanthrene (MC)","volume":"19","author":"Jewell","year":"1989","journal-title":"Xenobiotica"},{"key":"10.1006\/taap.1999.8669_RF23","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/0141-1136(89)90187-6","article-title":"Induction of the hepatic microsomal mixed-function oxygenase (MFO) system of Alligator mississippiensis by 3-methylcholanthrene (3-MC)","volume":"28","author":"Jewell","year":"1989","journal-title":"Marine Environ. Res."},{"key":"10.1006\/taap.1999.8669_RF24","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/jez.1402700111","article-title":"Role of P-450 aromatase in sex determination of the diamondback terrapin, Malaclemys terrapin","volume":"270","author":"Jeyasuria","year":"1994","journal-title":"J. Exp. Zool."},{"key":"10.1006\/taap.1999.8669_RF25","series-title":"Polycyclic Aromatic Compounds: Synthesis, Properties, Analytical Measurements, Occurrence and Biological Effects","first-page":"1095","article-title":"Benzo[a]pyrene metabolism by the american alligator, Alligator mississippiensis: Effects of 3-methylcholanthrene-pretreatment","author":"Kirchin","year":"1993"},{"key":"10.1006\/taap.1999.8669_RF26","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1006\/taap.1999.8669_RF27","first-page":"A824","article-title":"Cloning, sequencing and tissue expression of the first CYP3A form from fish (CYP3A27) in rainbow trout","volume":"11","author":"Lee","year":"1997","journal-title":"Fed. Proc."},{"key":"10.1006\/taap.1999.8669_RF28","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1006\/abio.1993.1504","article-title":"A fluorescence based protein assay for use with a microplate reader","volume":"214","author":"Lorenzen","year":"1993","journal-title":"Anal. Biochem."},{"key":"10.1006\/taap.1999.8669_RF29","series-title":"Agriculture and natural Resources","year":"1995"},{"key":"10.1006\/taap.1999.8669_RF30","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1046\/j.1365-2885.1998.00134.x","article-title":"Antibiotic effects on cytochromes P450 content and mixed-function oxygenase (MFO) activities in the American alligator, Alligator mississippiensis","volume":"21","author":"Mayeaux","year":"1998","journal-title":"J. Vet. Pharmacol. Ther."},{"key":"10.1006\/taap.1999.8669_RF31","first-page":"6","article-title":"An overview of current programs on drug development and regulation for aquaculture: Regional Aquaculture Center perspective","volume":"33","author":"McNabb","year":"1991","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF32","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0003-9861(89)90584-5","article-title":"Purification and characterization of hepatic steroid hydroxylases from untreated rainbow trout","volume":"268","author":"Miranda","year":"1989","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/taap.1999.8669_RF33","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0167-4838(90)90161-8","article-title":"Immunological characterization of constitutive isozymes of cytochrome P-450 from rainbow trout. Evidence for homology with phenobarbital-induced rat P-450s","volume":"1037","author":"Miranda","year":"1990","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1006\/taap.1999.8669_RF34","first-page":"9","article-title":"Compliance issues and proper animal drug use in aquaculture","volume":"33","author":"Mitchell","year":"1991","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00008571-199602000-00002","article-title":"P450 superfamily: Update on new sequences, gene mapping, accession numbers and nomenclature","volume":"6","author":"Nelson","year":"1996","journal-title":"Pharmacogenetics"},{"key":"10.1006\/taap.1999.8669_RF36","doi-asserted-by":"crossref","first-page":"484","DOI":"10.7589\/0090-3558-22.4.484","article-title":"Gram-negative septicemia in American alligators (Alligator mississippiensis)","volume":"22","author":"Novak","year":"1986","journal-title":"J. Wildl. Dis."},{"key":"10.1006\/taap.1999.8669_RF37","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1016\/S0021-9258(20)82245-5","article-title":"The carbon monoxide binding pigment of liver microsomes","volume":"239","author":"Omura","year":"1964","journal-title":"J. Biol. Chem."},{"key":"10.1006\/taap.1999.8669_RF38","first-page":"233","article-title":"Review and perspective on the use of mixed-function oxygenase enzymes in biological monitoring","volume":"86C","author":"Payne","year":"1987","journal-title":"Comp. Biochem. Physiol."},{"key":"10.1006\/taap.1999.8669_RF39","doi-asserted-by":"crossref","first-page":"67","DOI":"10.7589\/0090-3558-24.1.67","article-title":"Bone morphometrics and tetracycline marking patterns in young growing American alligators (Alligator mississippiensis)","volume":"24","author":"Roberts","year":"1988","journal-title":"J. Wildl. Dis."},{"key":"10.1006\/taap.1999.8669_RF40","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0141-1136(89)90197-9","article-title":"Differential expression of multiple forms of cytochrome P-450 in vertebrates: Antibodies to purified rat cytochrome P-450s as molecular probes for the evolution of P-450 gene families I and II","volume":"28","author":"Ronis","year":"1989","journal-title":"Mar. Environ. Res."},{"key":"10.1006\/taap.1999.8669_RF41","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/j.1365-2885.1995.tb00596.x","article-title":"The effects of enrofloxacin on hepatic microsomal mixed function oxidases in broiler chickens","volume":"18","author":"Schlosberg","year":"1995","journal-title":"J. Vet. Pharmacol. Ther."},{"key":"10.1006\/taap.1999.8669_RF42","first-page":"4","article-title":"An overview of current programs on drug development and regulation for aquaculture: U.S. Fish and Wildlife Service perspective","volume":"33","author":"Schnick","year":"1991","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF43","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/jez.1402700113","article-title":"Molecular mechanisms of TSD in reptiles: A search for the magic bullet","volume":"270","author":"Spotila","year":"1994","journal-title":"J. Exp. Zool."},{"key":"10.1006\/taap.1999.8669_RF44","series-title":"Aquatic Toxicology: Molecular, Biochemical, and Cellular Perspectives","first-page":"87","article-title":"Biochemistry and molecular biology of monooxygenases: Current perspectives on forms, functions, and regulation of cytochrome P450 in aquatic species","author":"Stegeman","year":"1994"},{"key":"10.1006\/taap.1999.8669_RF45","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets, procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Nat. Acad. Sci. USA"},{"key":"10.1006\/taap.1999.8669_RF46","first-page":"254","article-title":"In vitro and in vivo study of the effects of enrofloxacin on hepatic cytochrome P-450: Potential for drug interactions","volume":"38","author":"Vancutsem","year":"1996","journal-title":"Vet. Hum. Toxicol."},{"key":"10.1006\/taap.1999.8669_RF47","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1042\/bst0190746","article-title":"Microsomal activation of benzo[a]pyrene by Alligator mississippiensis: Mechanisms, mutagenicity and induction","volume":"19","author":"Winston","year":"1991","journal-title":"Biochem. Soc. Trans."},{"key":"10.1006\/taap.1999.8669_RF48","series-title":"The Seventh International Congress of Toxicology, July 2\u20136","article-title":"cDNA cloning and characterization of a novel cytochrome P450 from rainbow trout","author":"Yang","year":"1995"},{"key":"10.1006\/taap.1999.8669_RF49","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1006\/abbi.1998.0607","article-title":"CYP2M1: Cloning, sequencing, and expression of a new cytochrome P450 from rainbow trout liver with fatty acid (omega-6)-hydroxylation activity","volume":"352","author":"Yang","year":"1998","journal-title":"Arch. Biochem. Biophys."},{"key":"10.1006\/taap.1999.8669_RF50","doi-asserted-by":"crossref","first-page":"457","DOI":"10.2166\/wst.1993.0582","article-title":"The effect of PCB on the induction of cytochrome P450 enzyme, detected by monoclonal antibody against the P450IA1 gene products, in barn owl and marsh turtle hepatic microsomes","volume":"27","author":"Yawetz","year":"1993","journal-title":"Water Sci. Technol."},{"key":"10.1006\/taap.1999.8669_RF51","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0141-1136(93)90047-4","article-title":"Induction, fractionation, and localization of cytochrome P450 isozymes in the liver of the freshwater turtle, Chrysemys picta picta","volume":"35","author":"Yawetz","year":"1993","journal-title":"Mar. Environ. Res."}],"container-title":["Toxicology and Applied Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X99986696?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041008X99986696?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T09:03:23Z","timestamp":1620032603000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041008X99986696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,6]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,6]]}},"alternative-id":["S0041008X99986696"],"URL":"https:\/\/doi.org\/10.1006\/taap.1999.8669","relation":{},"ISSN":["0041-008X"],"issn-type":[{"value":"0041-008X","type":"print"}],"subject":[],"published":{"date-parts":[[1999,6]]}}}