{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:45:41Z","timestamp":1761842741176,"version":"3.44.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[1998,6,1]],"date-time":"1998-06-01T00:00:00Z","timestamp":896659200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":5525,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[1998,6]]},"DOI":"10.1006\/viro.1998.9172","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T19:24:08Z","timestamp":1033932248000},"page":"241-249","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":45,"title":["Direct Cleavage of eIF4G by Poliovirus 2A Protease Is Inefficientin Vitro"],"prefix":"10.1006","volume":"245","author":[{"given":"Michael L.","family":"Bovee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry J.","family":"Lamphear","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Rhoads","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard E.","family":"Lloyd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/viro.1998.9172_VY989172RF1","doi-asserted-by":"crossref","first-page":"3587","DOI":"10.1002\/j.1460-2075.1993.tb06032.x","article-title":"Poliovirus RNA synthesis utilizes an RNP complex formed around the 5\u2032-end of viral RNA","volume":"12","author":"Andino","year":"1993","journal-title":"EMBO J."},{"key":"10.1006\/viro.1998.9172_VY989172RF2","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1128\/mr.60.3.499-511.1996","article-title":"RNA\u2013Protein interactions in regulation of picornavirus RNA translation","volume":"60","author":"Belsham","year":"1996","journal-title":"Microbiol Rev."},{"key":"10.1006\/viro.1998.9172_VY989172RF3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1006\/viro.1995.1541","article-title":"K562 cell strains differ in their response to poliovirus infection","volume":"213","author":"Benton","year":"1995","journal-title":"Virology"},{"key":"10.1006\/viro.1998.9172_VY989172RF4","first-page":"2913","article-title":"Poliovirus mutant that does not selectively inhibit host cell protein synthesis","volume":"5","author":"Bernstein","year":"1985","journal-title":"Mol. Cell. Biol."},{"key":"10.1006\/viro.1998.9172_VY989172RF5","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1128\/jvi.61.4.986-991.1987","article-title":"Proteolysis of the p220 component of the cap-binding protein complex is not sufficient for complete inhibition of host cell protein synthesis after poliovirus infection","volume":"61","author":"Bonneau","year":"1987","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF6","doi-asserted-by":"crossref","first-page":"14714","DOI":"10.1016\/S0021-9258(18)98745-4","article-title":"The effect of poliovirus proteinase 2Apro expression on cellular metabolism","volume":"266","author":"Davies","year":"1991","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF7","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1128\/jvi.54.2.634-638.1985","article-title":"Human rhinovirus 14 infection of HeLa cells results in the proteolytic cleavage of the p220 cao-binding complex subunit and inactivates globin mRNA translation in vitro","volume":"54","author":"Etchison","year":"1985","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF8","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1128\/jvi.51.3.832-837.1984","article-title":"Demonstration in vitro that eucaryotic initiation factor 3 is active but a cap-binding protein complex is inactive in poliovirus-infected HeLa cells","volume":"51","author":"Etchison","year":"1984","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF9","doi-asserted-by":"crossref","first-page":"14806","DOI":"10.1016\/S0021-9258(18)33352-0","article-title":"Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eukaryotic initiation factor 3 and a cap binding protein complex","volume":"257","author":"Etchison","year":"1982","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF10","doi-asserted-by":"crossref","first-page":"7492","DOI":"10.1016\/S0021-9258(19)39140-9","article-title":"Variations in cap-binding complexes from uninfected and poliovirus-infected HeLa cells","volume":"265","author":"Etchison","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF11","doi-asserted-by":"crossref","first-page":"5804","DOI":"10.1016\/S0021-9258(20)81965-6","article-title":"New initiation factor activity required for globin mRNA translation","volume":"258","author":"Grifo","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF12","doi-asserted-by":"crossref","first-page":"8444","DOI":"10.1128\/jvi.70.12.8444-8450.1996","article-title":"The eIF4G\u2013eIF4E complex is the target for direct cleavage by the rhinovirus 2A proteinase","volume":"70","author":"Haghighat","year":"1996","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF13","series-title":"Human Enterovirus Infections","article-title":"Translation and host cell shutoff","author":"Haller","year":"1995"},{"key":"10.1006\/viro.1998.9172_VY989172RF14","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.1128\/jvi.66.6.3330-3338.1992","article-title":"Determinants of substrate recognition by poliovirus 2A proteinase","volume":"66","author":"Hellen","year":"1992","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF15","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1099\/0022-1317-68-7-1835","article-title":"The complete nucleotide sequence of Coxsackievirus B4 and its comparison to other members of the Picornaviridae","volume":"68","author":"Jenkins","year":"1987","journal-title":"J. Gen. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF16","doi-asserted-by":"crossref","first-page":"5677","DOI":"10.1128\/jvi.68.9.5677-5684.1994","article-title":"Foot-and-mouth disease virus leader proteinase: Purification of the lb form and determination of its cleavage site on eIF-4 gamma","volume":"68","author":"Kirchweger","year":"1994","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF17","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1128\/jvi.61.9.2711-2718.1987","article-title":"Poliovirus proteinase 2A induces cleavage of eukaryotic initiation factor 4F polypeptide p220","volume":"61","author":"Kr\u00e4usslich","year":"1987","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF18","doi-asserted-by":"crossref","first-page":"5333","DOI":"10.1016\/S0021-9258(19)39358-5","article-title":"Cap binding protein complex that restores protein synthesis in heat-shocked Ehrlich cell lysates contains highly phosphorylated eIF-4E","volume":"265","author":"Lamphear","year":"1990","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF19","doi-asserted-by":"crossref","first-page":"15726","DOI":"10.1021\/bi961864t","article-title":"A single amino acid change in protein synthesis initiation factor 4G renders cap-dependent translation resistant to picornaviral 2A proteases","volume":"35","author":"Lamphear","year":"1996","journal-title":"Biochemistry"},{"key":"10.1006\/viro.1998.9172_VY989172RF20","doi-asserted-by":"crossref","first-page":"19200","DOI":"10.1016\/S0021-9258(19)36499-3","article-title":"Mapping the cleavage site in protein synthesis initiation factor-eIF-4\u03b3 of the 2A proteases from human coxsackievirus and rhinovirus","volume":"268","author":"Lamphear","year":"1993","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF21","doi-asserted-by":"crossref","first-page":"7581","DOI":"10.1021\/bi00080a033","article-title":"Purification of 2 Picornaviral-2A proteinases \u2014 Interaction with eIF-4-\u03b3 and influence on in vitro translation","volume":"32","author":"Liebig","year":"1993","journal-title":"Biochemistry"},{"key":"10.1006\/viro.1998.9172_VY989172RF22","doi-asserted-by":"crossref","first-page":"4216","DOI":"10.1128\/jvi.62.11.4216-4223.1988","article-title":"Relationship of p220 cleavage during picornavirus infection to 2A proteinase sequences","volume":"62","author":"Lloyd","year":"1988","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF23","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.1128\/jvi.61.8.2480-2488.1987","article-title":"Restriction of translation of capped mRNA in vitro as a model for poliovirus-induced inhibition of host cell protein synthesis: Relationship to p220 cleavage","volume":"61","author":"Lloyd","year":"1987","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0042-6822(86)90291-6","article-title":"Cleavage of the cap binding protein complex polypeptide p220 is not effected by the second poliovirus protease 2A","volume":"150","author":"Lloyd","year":"1986","journal-title":"Virology"},{"key":"10.1006\/viro.1998.9172_VY989172RF25","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0003-2697(81)90783-1","article-title":"Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity","volume":"117","author":"Morrisey","year":"1981","journal-title":"Anal. Biochem."},{"key":"10.1006\/viro.1998.9172_VY989172RF26","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/emboj\/16.4.844","article-title":"The proteolytic cleavage of eukaryotic initiation factor (eIF) 4G is prevented by eIF4E binding protein (PHAS-I, 4E-BP1) in the reticulocyte lysate","volume":"16","author":"Ohlmann","year":"1997","journal-title":"EMBO J."},{"key":"10.1006\/viro.1998.9172_VY989172RF27","doi-asserted-by":"crossref","first-page":"4887","DOI":"10.1073\/pnas.78.8.4887","article-title":"Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome","volume":"78","author":"Racaniello","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/viro.1998.9172_VY989172RF28","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1128\/jvi.69.5.2954-2961.1995","article-title":"Poliovirus infection enhances the formation of two ribonucleoprotein complexes at the 3\u2032 end of viral negative-strand RNA","volume":"69","author":"Roehl","year":"1995","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF29","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1093\/nar\/13.6.2111","article-title":"Human rhinovirus 2: Complete nucleotide sequence and proteolytic processing signals in the capsid region","volume":"6","author":"Skern","year":"1985","journal-title":"Nucleic Acids Res."},{"key":"10.1006\/viro.1998.9172_VY989172RF30","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1006\/viro.1994.1089","article-title":"2A proteinases of coxsackie- and rhinovirus cleave peptides derived from eIF-4\u03b3 via a common recognition motif","volume":"198","author":"Sommergruber","year":"1994","journal-title":"Virology"},{"key":"10.1006\/viro.1998.9172_VY989172RF31","doi-asserted-by":"crossref","first-page":"22639","DOI":"10.1016\/S0021-9258(18)41720-6","article-title":"Cleavage specificity on synthetic peptide substrates of human rhinovirus-2 Proteinase-2A","volume":"267","author":"Sommergruber","year":"1992","journal-title":"J. Biol. Chem."},{"key":"10.1006\/viro.1998.9172_VY989172RF32","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/0042-6822(89)90042-1","article-title":"Polypeptide 2A of human rhinovirus type 2: Identification as a protease and characterization by mutational analysis","volume":"169","author":"Sommergruber","year":"1989","journal-title":"Virology"},{"key":"10.1006\/viro.1998.9172_VY989172RF33","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1073\/pnas.86.7.2143","article-title":"Human immunodeficiency virus tat-activated expression of poliovirus protein 2A inhibits mRNA translation","volume":"86","author":"Sun","year":"1989","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/viro.1998.9172_VY989172RF34","doi-asserted-by":"crossref","first-page":"3605","DOI":"10.1128\/jvi.69.6.3605-3614.1995","article-title":"RNA\u2013protein interactions directed by the 3\u2032 end of human rhinovirus genomic RNA","volume":"69","author":"Todd","year":"1995","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF35","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1128\/jvi.66.5.2943-2951.1992","article-title":"Relationship of eukaryotic initiation factor 3 to poliovirus-induced p220 cleavage activity","volume":"66","author":"Wyckoff","year":"1992","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF36","doi-asserted-by":"crossref","first-page":"9529","DOI":"10.1073\/pnas.87.24.9529","article-title":"Eukaryotic initiation factor-3 is required for poliovirus-2A protease-induced cleavage of the p220 component of eukaryotic initiation factor-4F","volume":"87","author":"Wyckoff","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/viro.1998.9172_VY989172RF37","doi-asserted-by":"crossref","first-page":"6881","DOI":"10.1128\/jvi.71.9.6881-6886.1997","article-title":"Poliovirus-encoded protease 2Apro cleaves the TATA-binding protein but does not inhibit host cell RNA polymerase II transcription in vitro","volume":"71","author":"Yalamanchili","year":"1997","journal-title":"J. Virol."},{"key":"10.1006\/viro.1998.9172_VY989172RF38","doi-asserted-by":"crossref","first-page":"23226","DOI":"10.1016\/S0021-9258(18)50080-6","article-title":"Amino acid sequence of the human protein synthesis initiation factor eIF-4\u03b3","volume":"267","author":"Yan","year":"1992","journal-title":"J. Biol. Chem."}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682298991723?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682298991723?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T23:01:54Z","timestamp":1758582114000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682298991723"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,6]]}},"alternative-id":["S0042682298991723"],"URL":"https:\/\/doi.org\/10.1006\/viro.1998.9172","relation":{},"ISSN":["0042-6822"],"issn-type":[{"type":"print","value":"0042-6822"}],"subject":[],"published":{"date-parts":[[1998,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Direct Cleavage of eIF4G by Poliovirus 2A Protease Is Inefficientin Vitro","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1006\/viro.1998.9172","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 1998 Academic Press. All rights reserved.","name":"copyright","label":"Copyright"}]}}