{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T16:35:17Z","timestamp":1773160517074,"version":"3.50.1"},"reference-count":30,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2013,7,17]],"date-time":"2013-07-17T00:00:00Z","timestamp":1374019200000},"content-version":"vor","delay-in-days":4490,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Virology"],"published-print":{"date-parts":[[2001,4]]},"DOI":"10.1006\/viro.2001.0846","type":"journal-article","created":{"date-parts":[[2002,9,19]],"date-time":"2002-09-19T09:00:16Z","timestamp":1032426016000},"page":"288-300","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":52,"title":["Infectious cDNA Clone of Attenuated Langat Tick-Borne Flavivirus (Strain E5) and a 3\u2032 Deletion Mutant Constructed from It Exhibit Decreased Neuroinvasiveness in Immunodeficient Mice"],"prefix":"10.1006","volume":"282","author":[{"given":"Alexander G.","family":"Pletnev","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1006\/viro.2001.0846_RF1","doi-asserted-by":"crossref","first-page":"6433","DOI":"10.1128\/jvi.71.9.6433-6444.1997","article-title":"Translation elongation factor-1 alpha interacts with the 3\u2032 stem-loop region of West Nile virus genomic RNA","volume":"71","author":"Blackwell","year":"1997","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1099\/0022-1317-70-1-37","article-title":"Antigenic relationships between flaviviruses as determined by cross-neutralization test with polyclonal antisera","volume":"70","author":"Calisher","year":"1989","journal-title":"J. Gen. Virol."},{"key":"10.1006\/viro.2001.0846_RF3","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1006\/viro.2000.0220","article-title":"Infectious cDNA clones of Langat tick-borne flavivirus that differ from their parent in peripheral neurovirulence","volume":"269","author":"Campbell","year":"2000","journal-title":"Virology"},{"key":"10.1006\/viro.2001.0846_RF4","doi-asserted-by":"crossref","first-page":"3466","DOI":"10.1128\/jvi.71.5.3466-3473.1997","article-title":"RNA\u2013protein interactions: Involvement of NS3, NS5, and 3\u2032 noncoding regions of Japanese encephalitis virus genomic RNA","volume":"71","author":"Chen","year":"1997","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF5","first-page":"528","article-title":"A full-size DNA copy of the tick-borne encephalitis virus genome. Part I. Analysis of noncoding 5\u2032- and 3\u2032-regions","volume":"21","author":"Dobrikova","year":"1995","journal-title":"Bioorg. Chem."},{"key":"10.1006\/viro.2001.0846_RF6","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1006\/viro.1999.9695","article-title":"Immunogenicity, genetic stability, and protective efficacy of a recombinant, chimeric yellow fever-Japanese encephalitis virus (ChimeriVax-JE) as a live, attenuated vaccine candidate against Japanese encephalitis","volume":"257","author":"Guirakhoo","year":"1999","journal-title":"Virology"},{"key":"10.1006\/viro.2001.0846_RF7","first-page":"425","article-title":"Experience in the study of a life vaccine made from the TP21 strain of Malayan Langat virus","volume":"39","author":"Il'enko","year":"1968","journal-title":"Bull. WHO"},{"key":"10.1006\/viro.2001.0846_RF8","first-page":"39","article-title":"An improved micromethod for infectivity assays and neutralization test of dengue viruses","volume":"30","author":"Ishimine","year":"1987","journal-title":"Biken J."},{"key":"10.1006\/viro.2001.0846_RF9","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1093\/infdis\/169.3.512","article-title":"Analysis of a yellow fever virus isolated from a fatal case of vaccine-associated human encephalitis","volume":"169","author":"Jennings","year":"1994","journal-title":"J. Infect. Dis."},{"key":"10.1006\/viro.2001.0846_RF10","doi-asserted-by":"crossref","first-page":"4070","DOI":"10.1128\/jvi.65.8.4070-4077.1991","article-title":"Presence of poly(A) in a flavivirus: Significant differences between the 3\u2032 noncoding regions of the genomic RNAs of tick-borne encephalitis virus strains","volume":"65","author":"Mandl","year":"1991","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF11","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.1128\/JVI.72.3.2132-2140.1998","article-title":"Spontaneous and engineered deletions in the 3\u2032 noncoding region of tick-borne encephalitis virus: Construction of highly attenuated mutants of a flavivirus","volume":"72","author":"Mandl","year":"1998","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF12","first-page":"215","article-title":"Experimental live tick-borne encephalitis vaccine (Langat E5\u201c14\u201d virus clone): Volunteers 1 and 2 years after single-dose immunization","volume":"20","author":"Mayer","year":"1976","journal-title":"Acta Virol."},{"key":"10.1006\/viro.2001.0846_RF13","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1099\/0022-1317-78-11-2711","article-title":"The molecular basis of virulence of the encephalitogenic flaviviruses","volume":"78","author":"McMinn","year":"1997","journal-title":"J. Gen. Virol."},{"key":"10.1006\/viro.2001.0846_RF14","doi-asserted-by":"crossref","first-page":"3930","DOI":"10.1128\/jvi.70.6.3930-3937.1996","article-title":"Dengue type 4 virus mutants containing deletions in the 3\u2032 noncoding region of the RNA genome: Analysis of growth restriction in cell culture and altered viremia pattern and immunogenicity in rhesus monkeys","volume":"70","author":"Men","year":"1996","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF15","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/0264-410X(93)90329-V","article-title":"Possible association of encephalitis and 17D yellow fever vaccination in a 29-year-old traveller","volume":"11","author":"Merlo","year":"1993","journal-title":"Vaccine"},{"key":"10.1006\/viro.2001.0846_RF16","series-title":"Fields Virology","first-page":"961","article-title":"Flaviviruses","author":"Monath","year":"1996"},{"key":"10.1006\/viro.2001.0846_RF17","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/oxfordjournals.aje.a120858","article-title":"Histological studies of the monkey neurovirulence of group B arboviruses. IV. Evaluation of an attenuated strain (E5) of Langat virus","volume":"88","author":"Nathanson","year":"1968","journal-title":"Am. J. Epidemiol."},{"key":"10.1006\/viro.2001.0846_RF18","doi-asserted-by":"crossref","first-page":"10532","DOI":"10.1073\/pnas.89.21.10532","article-title":"Construction and characterization of tick-borne encephalitis\/dengue type 4 viruses","volume":"89","author":"Pletnev","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/viro.2001.0846_RF19","doi-asserted-by":"crossref","first-page":"4956","DOI":"10.1128\/jvi.67.8.4956-4963.1993","article-title":"Chimeric tick-borne encephalitis and dengue type 4 viruses: effects of mutations on neurovirulence in mice","volume":"67","author":"Pletnev","year":"1993","journal-title":"J. Virol."},{"key":"10.1006\/viro.2001.0846_RF20","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1073\/pnas.95.4.1746","article-title":"Attenuation of the Langat tick-borne flavivirus by chimerization with mosquito-borne flavivirus dengue type 4","volume":"95","author":"Pletnev","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1006\/viro.2001.0846_RF21","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1006\/viro.2000.0426","article-title":"Chimeric Langat\/dengue viruses protect mice from heterologous challenge with the highly virulent strains of tick-borne encephalitis virus","volume":"274","author":"Pletnev","year":"2000","journal-title":"Virology"},{"key":"10.1006\/viro.2001.0846_RF22","first-page":"89","article-title":"Vaccination of human volunteers against Russian spring-summer (RSS) virus complex with attenuated Langat E5 virus","volume":"42","author":"Price","year":"1970","journal-title":"Bull. WHO"},{"key":"10.1006\/viro.2001.0846_RF23","doi-asserted-by":"crossref","first-page":"100","DOI":"10.4269\/ajtmh.1973.22.100","article-title":"Immunization of mice against Russian spring\u2013summer virus complex and monkeys against Powassan virus with attenuated Langat E5 virus","volume":"22","author":"Price","year":"1973","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1006\/viro.2001.0846_RF24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/375291a0","article-title":"The envelope glycoprotein from tick-borne encephalitis virus at 2 \u00c5 resolution","volume":"375","author":"Rey","year":"1995","journal-title":"Nature"},{"key":"10.1006\/viro.2001.0846_RF25","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/178581a0","article-title":"A virus resembling Russian spring\u2013summer encephalitis virus from an Ixodid in Malaya","volume":"178","author":"Smith","year":"1956","journal-title":"Nature"},{"key":"10.1006\/viro.2001.0846_RF26","unstructured":"Smorodincev, A. A., and Dubov, A. V.1986. Live vaccines against tick-borne encephalitis. InTick-Borne Encephalitis and Its Vaccine Prophylaxis (A. A. Smorodincev, Ed.), pp. 190\u2013211. Meditsina, Leningrad."},{"key":"10.1006\/viro.2001.0846_RF27","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1093\/oxfordjournals.aje.a120633","article-title":"A chick embryo attenuated strain (TP21 E5) of Langat virus. I. Virulence of the virus for mice and monkeys","volume":"84","author":"Thind","year":"1966","journal-title":"Am. J. Epidemiol."},{"key":"10.1006\/viro.2001.0846_RF28","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/oxfordjournals.aje.a120634","article-title":"A chick embryo attenuated strain (TP21 E5) of Langat virus. II. Stability after passage in various laboratory animals and tissue culture","volume":"84","author":"Thind","year":"1966","journal-title":"Am. J. Epidemiol."},{"key":"10.1006\/viro.2001.0846_RF29","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1006\/viro.1995.1557","article-title":"The flavivirus 3\u2032-noncoding region: Extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus","volume":"213","author":"Wallner","year":"1995","journal-title":"Virology"},{"key":"10.1006\/viro.2001.0846_RF30","doi-asserted-by":"crossref","first-page":"7510","DOI":"10.1128\/JVI.72.9.7510-7522.1998","article-title":"Identification of specific nucleotide sequences within the conserved 3\u2032-SL in the dengue type 2 virus genome required for replication","volume":"72","author":"Zeng","year":"1998","journal-title":"J. Virol."}],"container-title":["Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682201908463?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0042682201908463?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T22:47:31Z","timestamp":1758581251000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0042682201908463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,4]]}},"alternative-id":["S0042682201908463"],"URL":"https:\/\/doi.org\/10.1006\/viro.2001.0846","relation":{},"ISSN":["0042-6822"],"issn-type":[{"value":"0042-6822","type":"print"}],"subject":[],"published":{"date-parts":[[2001,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Infectious cDNA Clone of Attenuated Langat Tick-Borne Flavivirus (Strain E5) and a 3\u2032 Deletion Mutant Constructed from It Exhibit Decreased Neuroinvasiveness in Immunodeficient Mice","name":"articletitle","label":"Article Title"},{"value":"Virology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1006\/viro.2001.0846","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"converted-article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2001 Academic Press. All rights reserved.","name":"copyright","label":"Copyright"}]}}