{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T03:48:08Z","timestamp":1783828088483,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":20,"publisher":"Springer New York","isbn-type":[{"value":"9781493912230","type":"print"},{"value":"9781493912247","type":"electronic"}],"license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014]]},"DOI":"10.1007\/7651_2014_72","type":"book-chapter","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T10:04:22Z","timestamp":1391594662000},"page":"203-218","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications"],"prefix":"10.1007","author":[{"given":"John J.","family":"Wille","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy J.","family":"Burdge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Y.","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,2,6]]},"reference":[{"key":"72_CR1","doi-asserted-by":"publisher","first-page":"650","DOI":"10.1111\/j.1365-2230.1986.tb00526.x","volume":"11","author":"IM Leigh","year":"1986","unstructured":"Leigh IM, Purkis PE (1986) Culture grafted leg ulcers. Clin Exp Dermatol 11:650\u2013652","journal-title":"Clin Exp Dermatol"},{"key":"72_CR2","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1001\/archderm.134.3.344","volume":"134","author":"TJ Philips","year":"1998","unstructured":"Philips TJ (1998) New skin for old: developments in biological. Arch Dermatol 134:344\u2013349","journal-title":"Arch Dermatol"},{"key":"72_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1089\/ten.2006.12.2407","volume":"12","author":"M Ehrenreich","year":"2006","unstructured":"Ehrenreich M, Ruszczak Z (2006) Update on tissue engineered biological dressings. Tissue Eng 12:1\u201318","journal-title":"Tissue Eng"},{"issue":"6","key":"72_CR4","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1016\/j.suc.2010.08.004","volume":"90","author":"M Limova","year":"2010","unstructured":"Limova M (2010) Advanced wound coverings: bioengineered skin and dermal substitutes. Surg Clin North Am 90(6):1237\u20131255","journal-title":"Surg Clin North Am"},{"issue":"4","key":"72_CR5","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1111\/j.1524-475X.2011.00702.x","volume":"19","author":"JJ Wille","year":"2011","unstructured":"Wille JJ, Burdge JJ, Pittelkow MR (2011) Rapid healing of chronic venous stasis leg ulcers treated by application of a novel serum-free cultured autologous epidermis. Wound Repair Regen 19(4):464\u2013474","journal-title":"Wound Repair Regen"},{"issue":"8","key":"72_CR6","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1111\/j.1600-0625.2010.01087.x","volume":"19","author":"C Dieckmann","year":"2010","unstructured":"Dieckmann C, Renner R, Milkova L, Simon J (2010) Regenerative medicine in dermatology: biomaterials, tissue engineering, stem cells, gene transfer and beyond. Exp Dermatol 19(8):697\u2013706","journal-title":"Exp Dermatol"},{"key":"72_CR7","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1046\/j.1524-475X.1999.00201.x","volume":"7","author":"V Falanga","year":"1999","unstructured":"Falanga V, Sabolinski M (1999) A bilayered living skin construct (APLIGRAF) accelerates complete closure of hard-to-heal venous ulcers. Wound Repair Regen 7:201\u2013207","journal-title":"Wound Repair Regen"},{"key":"72_CR8","doi-asserted-by":"publisher","first-page":"93","DOI":"10.2147\/ciia.2007.2.1.93","volume":"2","author":"L Zaulyanov","year":"2007","unstructured":"Zaulyanov L, Kirsner RS (2007) A review of a bi-layered living cell treatment (Apligraf) in the treatment of venous leg ulcers and diabetic foot ulcers. Clin Interv Aging 2:93\u201398","journal-title":"Clin Interv Aging"},{"key":"72_CR9","first-page":"1079","volume":"138","author":"TJ Philips","year":"2002","unstructured":"Philips TJ, Manzoor J, Rojas A, Isaacs C, Carson P, Sabolinski M et al (2002) The longevity of a bilayered skin substitute after application to venous ulcers. Arch Dermatol 138:1079\u20131081","journal-title":"Arch Dermatol"},{"key":"72_CR10","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1111\/wrr.12082","volume":"21","author":"CS Blok","year":"2013","unstructured":"Blok CS, Vink L, de Boer EM, van Montfrans C, van den Hoogenband HM, Mooij MC et al (2013) Autologous skin substitute for hard-to-heal ulcers: retrospective analysis of safety, applicability, and efficacy in an outpatient and hospitalized setting. Wound Repair Regen 21:667\u2013676","journal-title":"Wound Repair Regen"},{"issue":"5","key":"72_CR11","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1111\/bjd.12570","volume":"169","author":"G Chaby","year":"2013","unstructured":"Chaby G, Senet P, Ganry O, Caudron A, Thiullier D et al (2013) Prognostic factors associated with healing of venous leg ulcers: a multicenter, prospective, cohort study. Br J Dermatol 169(5):1106\u20131113","journal-title":"Br J Dermatol"},{"issue":"9846","key":"72_CR12","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1016\/S0140-6736(12)60644-8","volume":"380","author":"RS Kirsner","year":"2012","unstructured":"Kirsner RS, Marston W, Snyder RJ, Lee TD, Cargill DI, Slade HB (2012) Spray-applied cell therapy with human allogeneic fibroblasts and keratinocytes for the treatment of chronic venous leg ulcers: a phase 2, multicentre, double-blind, randomized, placebo-controlled trial. Lancet 380(9846):977\u2013985","journal-title":"Lancet"},{"issue":"12","key":"72_CR13","first-page":"351","volume":"23","author":"W Marston","year":"2011","unstructured":"Marston W (2011) Mixed arterial and venous ulcers. Wounds 23(12):351\u2013356","journal-title":"Wounds"},{"key":"72_CR14","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/S0190-9622(86)70048-0","volume":"14","author":"JM Hefton","year":"1986","unstructured":"Hefton JM, Caldwell D, Biozes DG, Balin AK, Carter DM (1986) Grafting of skin ulcers with cultured autologous epidermal cells. J Am Acad Dermatol 14:339\u2013405","journal-title":"J Am Acad Dermatol"},{"key":"72_CR15","first-page":"170","volume":"7","author":"M Limova","year":"1995","unstructured":"Limova M, Mauro T (1995) Treatment of leg ulcers with cultured epithelial autografts: treatment protocol and five years experience. Wounds 7:170\u2013180","journal-title":"Wounds"},{"key":"72_CR16","unstructured":"U.S. Patent No. 4,304,866 (1981) Transplantable sheets of living keratinous tissue"},{"key":"72_CR17","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1111\/1523-1747.ep12540422","volume":"81","author":"S Boyce","year":"1983","unstructured":"Boyce S, Ham R (1983) Calcium regulation of differentiation of normal human epidermal cells in a chemically and serum-free defined medium. J Invest Dermatol 81:33\u201340","journal-title":"J Invest Dermatol"},{"key":"72_CR18","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1002\/jcp.1041210106","volume":"121","author":"JJ Wille","year":"1984","unstructured":"Wille JJ, Pittelkow MR, Scott RE (1984) Integrated control of growth and differentiation of normal human prokeratinocytes in serum-free medium: clonal analysis, growth kinetics, and cell cycle studies. J Cell Physiol 121:31\u201344","journal-title":"J Cell Physiol"},{"key":"72_CR19","unstructured":"U.S. Patent No. 5,686,307 (11\/11\/97) Serum-free medium for use in the formation of a histologically-complete living human skin substitute"},{"key":"72_CR20","unstructured":"U.S. Patent No. 5,795,781 (8\/18\/1998) Cell competency solution for use in the formation of a histologically-complete living human skin substitute"}],"container-title":["Methods in Molecular Biology","Epidermal Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/7651_2014_72","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T20:48:27Z","timestamp":1675802907000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/7651_2014_72"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"ISBN":["9781493912230","9781493912247"],"references-count":20,"URL":"https:\/\/doi.org\/10.1007\/7651_2014_72","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]},"assertion":[{"value":"6 February 2014","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}