{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T12:27:15Z","timestamp":1749558435466},"publisher-location":"New York, NY","reference-count":17,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071601747"},{"type":"electronic","value":"9781071601754"}],"license":[{"start":{"date-parts":[[2019,12,1]],"date-time":"2019-12-01T00:00:00Z","timestamp":1575158400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-0716-0175-4_11","type":"book-chapter","created":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T11:13:55Z","timestamp":1575285235000},"page":"147-158","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas"],"prefix":"10.1007","author":[{"given":"Takae","family":"Kiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chai-An","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,12,1]]},"reference":[{"key":"11_CR1","doi-asserted-by":"publisher","first-page":"2819","DOI":"10.1093\/nar\/9.12.2819","volume":"9","author":"LM Angerer","year":"1981","unstructured":"Angerer LM, Angerer RC (1981) Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization. Nucleic Acids Res 9:2819\u20132840","journal-title":"Nucleic Acids Res"},{"key":"11_CR2","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1177\/41.2.8419457","volume":"41","author":"LI Larsson","year":"1993","unstructured":"Larsson LI, Hougaard DM (1993) Sensitive detection of rat gastrin mRNA by in situ hybridization with chemically biotinylated oligodeoxynucleotides: validation, quantitation, and double-staining studies. J Histochem Cytochem 41:157\u2013163","journal-title":"J Histochem Cytochem"},{"key":"11_CR3","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1126\/science.280.5363.585","volume":"280","author":"AM Femino","year":"1998","unstructured":"Femino AM, Fay FS, Fogarty K et al (1998) Visualization of single RNA transcripts in situ. Science 280:585\u2013590","journal-title":"Science"},{"key":"11_CR4","doi-asserted-by":"publisher","first-page":"S151","DOI":"10.1101\/gr.4.4.S151","volume":"4","author":"GJ Nuovo","year":"1995","unstructured":"Nuovo GJ (1995) In situ PCR: protocols and applications. PCR Methods Appl 4:S151\u2013S167","journal-title":"PCR Methods Appl"},{"key":"11_CR5","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1007\/s004180050319","volume":"110","author":"EJM Speel","year":"1998","unstructured":"Speel EJM, Saremaslani P, Roth J et al (1998) Improved mRNA in situ hybridization on formaldehyde-fixed and paraffin-embedded tissue using signal amplification with different haptenized tyramides. Histochem Cell Biol 110:571\u2013577","journal-title":"Histochem Cell Biol"},{"key":"11_CR6","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1177\/002215540104900507","volume":"49","author":"AN Player","year":"2001","unstructured":"Player AN, Shen LP, Kenny D et al (2001) Single-copy gene detection using branched DNA (bDNA) in situ hybridization. J Histochem Cytochem 49:603\u2013611","journal-title":"J Histochem Cytochem"},{"key":"11_CR7","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","volume":"14","author":"F Wang","year":"2012","unstructured":"Wang F, Flanagan J, Su N et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14:22\u201329","journal-title":"J Mol Diagn"},{"key":"11_CR8","doi-asserted-by":"publisher","first-page":"e96435","DOI":"10.1371\/journal.pone.0096435","volume":"9","author":"A Pronin","year":"2014","unstructured":"Pronin A, Levay K, Velmeshev D et al (2014) Expression of olfactory signaling genes in the eye. PLoS One 9:e96435","journal-title":"PLoS One"},{"key":"11_CR9","first-page":"110","volume":"21","author":"Z Smit-McBride","year":"2015","unstructured":"Smit-McBride Z, Oltjen SL, Radu RA et al (2015) Localization of complement factor H gene expression and protein distribution in the mouse outer retina. Mol Vis 21:110\u2013123","journal-title":"Mol Vis"},{"key":"11_CR10","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1186\/s13024-018-0287-z","volume":"13","author":"T Kiyama","year":"2018","unstructured":"Kiyama T, Chen CK, Wang SW et al (2018) Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis. Mol Neurodegener 13:56","journal-title":"Mol Neurodegener"},{"key":"11_CR11","first-page":"1366","volume":"20","author":"AJ Stempel","year":"2014","unstructured":"Stempel AJ, Morgans CW, Stout JT et al (2014) Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina. Mol Vis 20:1366\u20131373","journal-title":"Mol Vis"},{"key":"11_CR12","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s12915-014-0055-7","volume":"12","author":"T Gross-Thebing","year":"2014","unstructured":"Gross-Thebing T, Paksa A, Raz E (2014) Simultaneous high-resolution detection of multiple transcripts combined with localization of protein in whole-mount embryos. BMC Biol 12:55","journal-title":"BMC Biol"},{"key":"11_CR13","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.mod.2017.06.004","volume":"148","author":"JA Morrison","year":"2017","unstructured":"Morrison JA, McKinney MC, Kulesa PM (2017) Resolving in vivo gene expression during collective cell migration using an integrated RNAscope, immunohistochemistry and tissue clearing method. Mech Dev 148:100\u2013106","journal-title":"Mech Dev"},{"key":"11_CR14","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1002\/cne.21489","volume":"505","author":"LL Daniele","year":"2007","unstructured":"Daniele LL, Adams RF, Durante DE et al (2007) Novel distribution of junctional adhesion molecule-C in the neural retina and retinal pigment epithelium. J Comp Neurol 505:166\u2013176","journal-title":"J Comp Neurol"},{"key":"11_CR15","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1038\/nature06739","volume":"452","author":"IJ Kim","year":"2008","unstructured":"Kim IJ, Zhang Y, Yamagata M et al (2008) Molecular identification of a retinal cell type that responds to upward motion. Nature 452:478\u2013482","journal-title":"Nature"},{"key":"11_CR16","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1002\/cne.20970","volume":"497","author":"S Hatter","year":"2006","unstructured":"Hatter S, Kumar M, Park A et al (2006) Central projections of melanopsin-expressing retinal ganglion cells in the mouse. J Comp Neurol 497:326\u2013349","journal-title":"J Comp Neurol"},{"key":"11_CR17","doi-asserted-by":"publisher","first-page":"13083","DOI":"10.1523\/JNEUROSCI.1027-14.2014","volume":"34","author":"CA Mao","year":"2014","unstructured":"Mao CA, Li H, Zhang Z et al (2014) T-box transcription regulator Tbr2 is essential for the formation and maintenance of Opn4\/melanopsin-expressing intrinsically photosensitive retinal ganglion cells. J Neurosci 34:13083\u201313095","journal-title":"J Neurosci"}],"container-title":["Methods in Molecular Biology","Retinal Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0175-4_11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,12,2]],"date-time":"2019-12-02T11:33:55Z","timestamp":1575286435000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-0175-4_11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,1]]},"ISBN":["9781071601747","9781071601754"],"references-count":17,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0175-4_11","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2019,12,1]]},"assertion":[{"value":"1 December 2019","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}