{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T06:52:27Z","timestamp":1784357547067,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":9,"publisher":"Springer US","isbn-type":[{"value":"9781071602652","type":"print"},{"value":"9781071602669","type":"electronic"}],"license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-0716-0266-9_16","type":"book-chapter","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T12:03:04Z","timestamp":1578916984000},"page":"193-203","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Retroviral Gene Transduction into T Cell Progenitors for Analysis of T Cell Development in the Thymus"],"prefix":"10.1007","author":[{"given":"Ryunosuke","family":"Muro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Takayanagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Nitta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"key":"16_CR1","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1146\/annurev-immunol-051116-052320","volume":"35","author":"J Abramson","year":"2017","unstructured":"Abramson J, Anderson G (2017) Thymic epithelial cells. Annu Rev Immunol 35:85\u2013118. \nhttps:\/\/doi.org\/10.1146\/annurev-immunol-051116-052320","journal-title":"Annu Rev Immunol"},{"issue":"9","key":"16_CR2","doi-asserted-by":"publisher","first-page":"5940","DOI":"10.4049\/jimmunol.181.9.5940","volume":"181","author":"K Shibata","year":"2008","unstructured":"Shibata K, Yamada H, Nakamura R et al (2008) Identification of CD25+ gamma delta T cells as fetal thymus-derived naturally occurring IL-17 producers. J Immunol 181(9):5940\u20135947","journal-title":"J Immunol"},{"key":"16_CR3","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1038\/sj.gt.3301206","volume":"7","author":"S Morita","year":"2000","unstructured":"Morita S, Kojima T, Kitamura T (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7:1063\u20131066. \nhttps:\/\/doi.org\/10.1038\/sj.gt.3301206","journal-title":"Gene Ther"},{"issue":"5","key":"16_CR4","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1016\/j.immuni.2018.09.010","volume":"49","author":"NA Spidale","year":"2018","unstructured":"Spidale NA, Sylvia K, Narayan K et al (2018) Interleukin-17-producing \u03b3\u03b4 T cells originate from SOX13. Immunity 49(5):857\u2013872.e5. \nhttps:\/\/doi.org\/10.1016\/j.immuni.2018.09.010","journal-title":"Immunity"},{"issue":"1","key":"16_CR5","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1172\/JCI95837","volume":"128","author":"R Muro","year":"2018","unstructured":"Muro R, Nitta T, Nakano K et al (2018) \u03b3\u03b4TCR recruits the Syk\/PI3K axis to drive proinflammatory differentiation program. J Clin Invest 128(1):415\u2013426. \nhttps:\/\/doi.org\/10.1172\/JCI95837","journal-title":"J Clin Invest"},{"key":"16_CR6","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aan5165","volume":"2","author":"T Nitta","year":"2017","unstructured":"Nitta T, Kochi Y, Muro R et al (2017) Human thymoproteasome variations influence CD8 T cell selection. Sci Immunol 2:eaan5165. \nhttps:\/\/doi.org\/10.1126\/sciimmunol.aan5165","journal-title":"Sci Immunol"},{"key":"16_CR7","doi-asserted-by":"publisher","first-page":"1149","DOI":"10.1084\/jem.176.4.1149","volume":"176","author":"RG Hawley","year":"1992","unstructured":"Hawley RG, Fong AZC, Burns BF et al (1992) Transplantable myeloproliferative disease induced in mice by interleukin 6 retrovirus. J Exp Med 176:1149\u20131163","journal-title":"J Exp Med"},{"key":"16_CR8","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1007\/978-1-62703-128-8_6","volume":"946","author":"T Nitta","year":"2013","unstructured":"Nitta T, Ohigashi I, Takahama Y (2013) The development of T lymphocytes in fetal thymus organ culture. Methods Mol Biol 946:85\u2013102. \nhttps:\/\/doi.org\/10.1007\/978-1-62703-128-8_6","journal-title":"Methods Mol Biol"},{"key":"16_CR9","doi-asserted-by":"publisher","unstructured":"Jenkinson W, Jenkinson E, Anderson G (2008) Preparation of 2-dGuo-treated thymus organ cultures. J Vis Exp. \nhttps:\/\/doi.org\/10.3791\/906","DOI":"10.3791\/906"}],"container-title":["Methods in Molecular Biology","T-Cell Receptor Signaling"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0266-9_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T12:14:09Z","timestamp":1578917649000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-0266-9_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"ISBN":["9781071602652","9781071602669"],"references-count":9,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0266-9_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"14 January 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}