{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T14:27:34Z","timestamp":1726064854598},"publisher-location":"New York, NY","reference-count":48,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071602652"},{"type":"electronic","value":"9781071602669"}],"license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-0716-0266-9_18","type":"book-chapter","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T12:03:04Z","timestamp":1578916984000},"page":"221-238","update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Investigating T Cell Receptor Signals In Situ by Two-Photon Microscopy of Thymocytes Expressing Genetic Reporters in Low-Density Chimeras"],"prefix":"10.1007","author":[{"given":"Marilaine","family":"Fournier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengqi","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heather J.","family":"Melichar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,1,14]]},"reference":[{"issue":"6089","key":"18_CR1","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1126\/science.1221063","volume":"336","author":"RN Germain","year":"2012","unstructured":"Germain RN, Robey EA, Cahalan MD (2012) A decade of imaging cellular motility and interaction dynamics in the immune system. Science 336(6089):1676\u20131681. https:\/\/doi.org\/10.1126\/science.1221063","journal-title":"Science"},{"key":"18_CR2","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1201\/9781315371689-2","volume-title":"Signaling Mechanisms Regulating T Cell Diversity and Function","author":"Nathalie Labrecque","year":"2017","unstructured":"Labrecque N, Dong M, Sood A, Melichar HJ (2018) In situ analysis of T cell receptor signals during positive selection. In: Soboloff J, Kappes DJ (eds) Signaling mechanisms regulating T cell diversity and function. CRC press\/Taylor & Francis, Boca Raton, FL, pp 17\u201340 https:\/\/doi.org\/10.1201\/9781315371689-2"},{"key":"18_CR3","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1146\/annurev-immunol-042617-053335","volume":"36","author":"BB Au-Yeung","year":"2018","unstructured":"Au-Yeung BB, Shah NH, Shen L, Weiss A (2018) ZAP-70 in signaling, biology, and disease. Annu Rev Immunol 36:127\u2013156. https:\/\/doi.org\/10.1146\/annurev-immunol-042617-053335","journal-title":"Annu Rev Immunol"},{"issue":"32","key":"18_CR4","doi-asserted-by":"publisher","first-page":"eaal2201","DOI":"10.1126\/sciimmunol.aal2201","volume":"4","author":"SC Neier","year":"2019","unstructured":"Neier SC, Ferrer A, Wilton KM, Smith SEP, Kelcher AMH, Pavelko KD, Canfield JM, Davis TR, Stiles RJ, Chen Z, McCluskey J, Burrows SR, Rossjohn J, Hebrink DM, Carmona EM, Limper AH, Kappes DJ, Wettstein PJ, Johnson AJ, Pease LR, Daniels MA, Neuhauser C, Gil D, Schrum AG (2019) The early proximal alphabeta TCR signalosome specifies thymic selection outcome through a quantitative protein interaction network. Sci Immunol 4(32):eaal2201. https:\/\/doi.org\/10.1126\/sciimmunol.aal2201","journal-title":"Sci Immunol"},{"issue":"7120","key":"18_CR5","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1038\/nature05269","volume":"444","author":"MA Daniels","year":"2006","unstructured":"Daniels MA, Teixeiro E, Gill J, Hausmann B, Roubaty D, Holmberg K, Werlen G, Hollander GA, Gascoigne NR, Palmer E (2006) Thymic selection threshold defined by compartmentalization of Ras\/MAPK signalling. Nature 444(7120):724\u2013729. https:\/\/doi.org\/10.1038\/nature05269","journal-title":"Nature"},{"issue":"4","key":"18_CR6","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1038\/79766","volume":"1","author":"NA Dower","year":"2000","unstructured":"Dower NA, Stang SL, Bottorff DA, Ebinu JO, Dickie P, Ostergaard HL, Stone JC (2000) RasGRP is essential for mouse thymocyte differentiation and TCR signaling. Nat Immunol 1(4):317\u2013321. https:\/\/doi.org\/10.1038\/79766","journal-title":"Nat Immunol"},{"issue":"14","key":"18_CR7","doi-asserted-by":"publisher","first-page":"2748","DOI":"10.1128\/MCB.00317-12","volume":"32","author":"RL Kortum","year":"2012","unstructured":"Kortum RL, Sommers CL, Pinski JM, Alexander CP, Merrill RK, Li W, Love PE, Samelson LE (2012) Deconstructing Ras signaling in the thymus. Mol Cell Biol 32(14):2748\u20132759. https:\/\/doi.org\/10.1128\/MCB.00317-12","journal-title":"Mol Cell Biol"},{"issue":"5","key":"18_CR8","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/S1074-7613(02)00451-X","volume":"17","author":"JJ Priatel","year":"2002","unstructured":"Priatel JJ, Teh SJ, Dower NA, Stone JC, Teh HS (2002) RasGRP1 transduces low-grade TCR signals which are critical for T cell development, homeostasis, and differentiation. Immunity 17(5):617\u2013627","journal-title":"Immunity"},{"issue":"9","key":"18_CR9","doi-asserted-by":"publisher","first-page":"4966","DOI":"10.4049\/jimmunol.167.9.4966","volume":"167","author":"S Mariathasan","year":"2001","unstructured":"Mariathasan S, Zakarian A, Bouchard D, Michie AM, Zuniga-Pflucker JC, Ohashi PS (2001) Duration and strength of extracellular signal-regulated kinase signals are altered during positive versus negative thymocyte selection. J Immunol 167(9):4966\u20134973","journal-title":"J Immunol"},{"issue":"1","key":"18_CR10","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1084\/jem.184.1.9","volume":"184","author":"J Alberola-Ila","year":"1996","unstructured":"Alberola-Ila J, Hogquist KA, Swan KA, Bevan MJ, Perlmutter RM (1996) Positive and negative selection invoke distinct signaling pathways. J Exp Med 184(1):9\u201318","journal-title":"J Exp Med"},{"issue":"8","key":"18_CR11","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1084\/jem.20150110","volume":"212","author":"Q Zou","year":"2015","unstructured":"Zou Q, Jin J, Xiao Y, Hu H, Zhou X, Jie Z, Xie X, Li JY, Cheng X, Sun SC (2015) T cell development involves TRAF3IP3-mediated ERK signaling in the Golgi. J Exp Med 212(8):1323\u20131336. https:\/\/doi.org\/10.1084\/jem.20150110","journal-title":"J Exp Med"},{"issue":"12","key":"18_CR12","doi-asserted-by":"crossref","first-page":"4594","DOI":"10.4049\/jimmunol.156.12.4594","volume":"156","author":"LP Kane","year":"1996","unstructured":"Kane LP, Hedrick SM (1996) A role for calcium influx in setting the threshold for CD4+CD8+ thymocyte negative selection. J Immunol 156(12):4594\u20134601","journal-title":"J Immunol"},{"issue":"9","key":"18_CR13","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1038\/ni.2379","volume":"13","author":"WL Lo","year":"2012","unstructured":"Lo WL, Donermeyer DL, Allen PM (2012) A voltage-gated sodium channel is essential for the positive selection of CD4(+) T cells. Nat Immunol 13(9):880\u2013887. https:\/\/doi.org\/10.1038\/ni.2379","journal-title":"Nat Immunol"},{"issue":"6","key":"18_CR14","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1038\/nri1632","volume":"5","author":"F Macian","year":"2005","unstructured":"Macian F (2005) NFAT proteins: key regulators of T-cell development and function. Nat Rev Immunol 5(6):472\u2013484. https:\/\/doi.org\/10.1038\/nri1632","journal-title":"Nat Rev Immunol"},{"issue":"297","key":"18_CR15","doi-asserted-by":"publisher","first-page":"ra92","DOI":"10.1126\/scisignal.2004400","volume":"6","author":"HJ Melichar","year":"2013","unstructured":"Melichar HJ, Ross JO, Herzmark P, Hogquist KA, Robey EA (2013) Distinct temporal patterns of T cell receptor signaling during positive versus negative selection in situ. Sci Signal 6(297):ra92. https:\/\/doi.org\/10.1126\/scisignal.2004400","journal-title":"Sci Signal"},{"issue":"7","key":"18_CR16","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1038\/ni.2918","volume":"15","author":"BB Au-Yeung","year":"2014","unstructured":"Au-Yeung BB, Melichar HJ, Ross JO, Cheng DA, Zikherman J, Shokat KM, Robey EA, Weiss A (2014) Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development. Nat Immunol 15(7):687\u2013694. https:\/\/doi.org\/10.1038\/ni.2918","journal-title":"Nat Immunol"},{"issue":"25","key":"18_CR17","doi-asserted-by":"publisher","first-page":"E2550","DOI":"10.1073\/pnas.1408482111","volume":"111","author":"JO Ross","year":"2014","unstructured":"Ross JO, Melichar HJ, Au-Yeung BB, Herzmark P, Weiss A, Robey EA (2014) Distinct phases in the positive selection of CD8+ T cells distinguished by intrathymic migration and T-cell receptor signaling patterns. Proc Natl Acad Sci U S A 111(25):E2550\u2013E2558. https:\/\/doi.org\/10.1073\/pnas.1408482111","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"5","key":"18_CR18","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1016\/j.immuni.2008.09.014","volume":"29","author":"PJ Ebert","year":"2008","unstructured":"Ebert PJ, Ehrlich LI, Davis MM (2008) Low ligand requirement for deletion and lack of synapses in positive selection enforce the gauntlet of thymic T cell maturation. Immunity 29(5):734\u2013745. https:\/\/doi.org\/10.1016\/j.immuni.2008.09.014","journal-title":"Immunity"},{"issue":"2","key":"18_CR19","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/ni1161","volume":"6","author":"NR Bhakta","year":"2005","unstructured":"Bhakta NR, Oh DY, Lewis RS (2005) Calcium oscillations regulate thymocyte motility during positive selection in the three-dimensional thymic environment. Nat Immunol 6(2):143\u2013151. https:\/\/doi.org\/10.1038\/ni1161","journal-title":"Nat Immunol"},{"issue":"3","key":"18_CR20","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.4049\/jimmunol.179.3.1586","volume":"179","author":"SH Wei","year":"2007","unstructured":"Wei SH, Safrina O, Yu Y, Garrod KR, Cahalan MD, Parker I (2007) Ca2+ signals in CD4+ T cells during early contacts with antigen-bearing dendritic cells in lymph node. J Immunol 179(3):1586\u20131594","journal-title":"J Immunol"},{"issue":"8","key":"18_CR21","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1038\/ni1490","volume":"8","author":"D Skokos","year":"2007","unstructured":"Skokos D, Shakhar G, Varma R, Waite JC, Cameron TO, Lindquist RL, Schwickert T, Nussenzweig MC, Dustin ML (2007) Peptide-MHC potency governs dynamic interactions between T cells and dendritic cells in lymph nodes. Nat Immunol 8(8):835\u2013844. https:\/\/doi.org\/10.1038\/ni1490","journal-title":"Nat Immunol"},{"issue":"4","key":"18_CR22","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.4049\/jimmunol.1502414","volume":"196","author":"M Borgne Le","year":"2016","unstructured":"Le Borgne M, Raju S, Zinselmeyer BH, Le VT, Li J, Wang Y, Miller MJ, Shaw AS (2016) Real-time analysis of calcium signals during the early phase of T cell activation using a genetically encoded calcium biosensor. J Immunol 196(4):1471\u20131479. https:\/\/doi.org\/10.4049\/jimmunol.1502414","journal-title":"J Immunol"},{"issue":"6","key":"18_CR23","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1038\/nm.3180","volume":"19","author":"M Mues","year":"2013","unstructured":"Mues M, Bartholomaus I, Thestrup T, Griesbeck O, Wekerle H, Kawakami N, Krishnamoorthy G (2013) Real-time in vivo analysis of T cell activation in the central nervous system using a genetically encoded calcium indicator. Nat Med 19(6):778\u2013783. https:\/\/doi.org\/10.1038\/nm.3180","journal-title":"Nat Med"},{"issue":"7","key":"18_CR24","doi-asserted-by":"publisher","first-page":"1787","DOI":"10.1016\/j.bbamcr.2013.01.011","volume":"1833","author":"V Perez Koldenkova","year":"2013","unstructured":"Perez Koldenkova V, Nagai T (2013) Genetically encoded Ca(2+) indicators: properties and evaluation. Biochim Biophys Acta 1833(7):1787\u20131797. https:\/\/doi.org\/10.1016\/j.bbamcr.2013.01.011","journal-title":"Biochim Biophys Acta"},{"issue":"6200","key":"18_CR25","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1126\/science.1257861","volume":"345","author":"Z Shulman","year":"2014","unstructured":"Shulman Z, Gitlin AD, Weinstein JS, Lainez B, Esplugues E, Flavell RA, Craft JE, Nussenzweig MC (2014) Dynamic signaling by T follicular helper cells during germinal center B cell selection. Science 345(6200):1058\u20131062. https:\/\/doi.org\/10.1126\/science.1257861","journal-title":"Science"},{"issue":"2","key":"18_CR26","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1038\/nmeth.2773","volume":"11","author":"T Thestrup","year":"2014","unstructured":"Thestrup T, Litzlbauer J, Bartholomaus I, Mues M, Russo L, Dana H, Kovalchuk Y, Liang Y, Kalamakis G, Laukat Y, Becker S, Witte G, Geiger A, Allen T, Rome LC, Chen TW, Kim DS, Garaschuk O, Griesinger C, Griesbeck O (2014) Optimized ratiometric calcium sensors for functional in vivo imaging of neurons and T lymphocytes. Nat Methods 11(2):175\u2013182. https:\/\/doi.org\/10.1038\/nmeth.2773","journal-title":"Nat Methods"},{"issue":"3","key":"18_CR27","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.ymeth.2008.09.025","volume":"46","author":"RM Paredes","year":"2008","unstructured":"Paredes RM, Etzler JC, Watts LT, Zheng W, Lechleiter JD (2008) Chemical calcium indicators. Methods 46(3):143\u2013151. https:\/\/doi.org\/10.1016\/j.ymeth.2008.09.025","journal-title":"Methods"},{"issue":"4","key":"18_CR28","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1038\/icb.2010.122","volume":"89","author":"HJ Melichar","year":"2011","unstructured":"Melichar HJ, Li O, Herzmark P, Padmanabhan RK, Oliaro J, Ludford-Menting MJ, Bousso P, Russell SM, Roysam B, Robey EA (2011) Quantifying subcellular distribution of fluorescent fusion proteins in cells migrating within tissues. Immunol Cell Biol 89(4):549\u2013557. https:\/\/doi.org\/10.1038\/icb.2010.122","journal-title":"Immunol Cell Biol"},{"issue":"2","key":"18_CR29","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.immuni.2012.09.012","volume":"38","author":"F Marangoni","year":"2013","unstructured":"Marangoni F, Murooka TT, Manzo T, Kim EY, Carrizosa E, Elpek NM, Mempel TR (2013) The transcription factor NFAT exhibits signal memory during serial T cell interactions with antigen-presenting cells. Immunity 38(2):237\u2013249. https:\/\/doi.org\/10.1016\/j.immuni.2012.09.012","journal-title":"Immunity"},{"issue":"8","key":"18_CR30","doi-asserted-by":"publisher","first-page":"3675","DOI":"10.1073\/pnas.0905901107","volume":"107","author":"GA Azar","year":"2010","unstructured":"Azar GA, Lemaitre F, Robey EA, Bousso P (2010) Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes. Proc Natl Acad Sci U S A 107(8):3675\u20133680. https:\/\/doi.org\/10.1073\/pnas.0905901107","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"18_CR31","doi-asserted-by":"publisher","first-page":"2733","DOI":"10.1084\/jem.20091201","volume":"207","author":"RS Friedman","year":"2010","unstructured":"Friedman RS, Beemiller P, Sorensen CM, Jacobelli J, Krummel MF (2010) Real-time analysis of T cell receptors in naive cells in vitro and in vivo reveals flexibility in synapse and signaling dynamics. J Exp Med 207(12):2733\u20132749. https:\/\/doi.org\/10.1084\/jem.20091201","journal-title":"J Exp Med"},{"key":"18_CR32","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/978-1-4939-2809-5_11","volume":"1323","author":"JO Ross","year":"2016","unstructured":"Ross JO, Melichar HJ, Halkias J, Robey EA (2016) Studying T cell development in thymic slices. Methods Mol Biol 1323:131\u2013140. https:\/\/doi.org\/10.1007\/978-1-4939-2809-5_11","journal-title":"Methods Mol Biol"},{"doi-asserted-by":"publisher","unstructured":"Sood A, Dong M, Melichar HJ (2016) Preparation and applications of organotypic thymic slice cultures. J Vis Exp 114. https:\/\/doi.org\/10.3791\/54355","key":"18_CR33","DOI":"10.3791\/54355"},{"key":"18_CR34","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/978-1-4939-6931-9_2","volume":"1591","author":"JN Lancaster","year":"2017","unstructured":"Lancaster JN, Ehrlich LI (2017) Analysis of thymocyte migration, cellular interactions, and activation by multiphoton fluorescence microscopy of live thymic slices. Methods Mol Biol 1591:9\u201325. https:\/\/doi.org\/10.1007\/978-1-4939-6931-9_2","journal-title":"Methods Mol Biol"},{"key":"18_CR35","doi-asserted-by":"publisher","first-page":"418","DOI":"10.3389\/fimmu.2017.00418","volume":"8","author":"I Calvo-Asensio","year":"2017","unstructured":"Calvo-Asensio I, Barthlott T, von Muenchow L, Lowndes NF, Ceredig R (2017) Differential response of mouse thymic epithelial cell types to ionizing radiation-induced DNA damage. Front Immunol 8:418. https:\/\/doi.org\/10.3389\/fimmu.2017.00418","journal-title":"Front Immunol"},{"issue":"5","key":"18_CR36","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1182\/blood.V98.5.1601","volume":"98","author":"B Chung","year":"2001","unstructured":"Chung B, Barbara-Burnham L, Barsky L, Weinberg K (2001) Radiosensitivity of thymic interleukin-7 production and thymopoiesis after bone marrow transplantation. Blood 98(5):1601\u20131606","journal-title":"Blood"},{"issue":"2","key":"18_CR37","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1182\/blood-2014-01-552794","volume":"124","author":"SL Zhang","year":"2014","unstructured":"Zhang SL, Wang X, Manna S, Zlotoff DA, Bryson JL, Blazar BR, Bhandoola A (2014) Chemokine treatment rescues profound T-lineage progenitor homing defect after bone marrow transplant conditioning in mice. Blood 124(2):296\u2013304. https:\/\/doi.org\/10.1182\/blood-2014-01-552794","journal-title":"Blood"},{"doi-asserted-by":"publisher","unstructured":"Dzhagalov IL, Melichar HJ, Ross JO, Herzmark P, Robey EA (2012) Two-photon imaging of the immune system. Curr Protoc Cytom Chapter 12: Unit12 26. https:\/\/doi.org\/10.1002\/0471142956.cy1226s60","key":"18_CR38","DOI":"10.1002\/0471142956.cy1226s60"},{"doi-asserted-by":"publisher","unstructured":"Ladi E, Herzmark P, Robey E (2008) In situ imaging of the mouse thymus using 2-photon microscopy. J Vis Exp 11. https:\/\/doi.org\/10.3791\/652","key":"18_CR39","DOI":"10.3791\/652"},{"issue":"6","key":"18_CR40","doi-asserted-by":"publisher","first-page":"e160","DOI":"10.1371\/journal.pbio.0030160","volume":"3","author":"CM Witt","year":"2005","unstructured":"Witt CM, Raychaudhuri S, Schaefer B, Chakraborty AK, Robey EA (2005) Directed migration of positively selected thymocytes visualized in real time. PLoS Biol 3(6):e160. https:\/\/doi.org\/10.1371\/journal.pbio.0030160","journal-title":"PLoS Biol"},{"issue":"8","key":"18_CR41","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/ni.1761","volume":"10","author":"M Borgne Le","year":"2009","unstructured":"Le Borgne M, Ladi E, Dzhagalov I, Herzmark P, Liao YF, Chakraborty AK, Robey EA (2009) The impact of negative selection on thymocyte migration in the medulla. Nat Immunol 10(8):823\u2013830. https:\/\/doi.org\/10.1038\/ni.1761","journal-title":"Nat Immunol"},{"key":"18_CR42","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1186\/1472-6750-2-11","volume":"2","author":"AK Hadjantonakis","year":"2002","unstructured":"Hadjantonakis AK, Macmaster S, Nagy A (2002) Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal. BMC Biotechnol 2:11","journal-title":"BMC Biotechnol"},{"issue":"12","key":"18_CR43","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.1038\/ni1139","volume":"5","author":"RL Lindquist","year":"2004","unstructured":"Lindquist RL, Shakhar G, Dudziak D, Wardemann H, Eisenreich T, Dustin ML, Nussenzweig MC (2004) Visualizing dendritic cell networks in vivo. Nat Immunol 5(12):1243\u20131250. https:\/\/doi.org\/10.1038\/ni1139","journal-title":"Nat Immunol"},{"issue":"8","key":"18_CR44","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1182\/blood.V97.8.2278","volume":"97","author":"DE Wright","year":"2001","unstructured":"Wright DE, Cheshier SH, Wagers AJ, Randall TD, Christensen JL, Weissman IL (2001) Cyclophosphamide\/granulocyte colony-stimulating factor causes selective mobilization of bone marrow hematopoietic stem cells into the blood after M phase of the cell cycle. Blood 97(8):2278\u20132285","journal-title":"Blood"},{"issue":"6","key":"18_CR45","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1016\/j.immuni.2009.09.020","volume":"31","author":"LI Ehrlich","year":"2009","unstructured":"Ehrlich LI, Oh DY, Weissman IL, Lewis RS (2009) Differential contribution of chemotaxis and substrate restriction to segregation of immature and mature thymocytes. Immunity 31(6):986\u2013998. https:\/\/doi.org\/10.1016\/j.immuni.2009.09.020","journal-title":"Immunity"},{"issue":"3","key":"18_CR46","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1084\/jem.184.3.923","volume":"184","author":"C Kurts","year":"1996","unstructured":"Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H (1996) Constitutive class I-restricted exogenous presentation of self antigens in vivo. J Exp Med 184(3):923\u2013930","journal-title":"J Exp Med"},{"issue":"10","key":"18_CR47","doi-asserted-by":"publisher","first-page":"3596","DOI":"10.1182\/blood.V89.10.3596","volume":"89","author":"TD Randall","year":"1997","unstructured":"Randall TD, Weissman IL (1997) Phenotypic and functional changes induced at the clonal level in hematopoietic stem cells after 5-fluorouracil treatment. Blood 89(10):3596\u20133606","journal-title":"Blood"},{"doi-asserted-by":"publisher","unstructured":"Swift S, Lorens J, Achacoso P, Nolan GP (2001) Rapid production of retroviruses for efficient gene delivery to mammalian cells using 293T cell-based systems. Curr Protoc Immunol Chapter 10: Unit 10 17C. https:\/\/doi.org\/10.1002\/0471142735.im1017cs31","key":"18_CR48","DOI":"10.1002\/0471142735.im1017cs31"}],"container-title":["Methods in Molecular Biology","T-Cell Receptor Signaling"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0266-9_18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T01:07:50Z","timestamp":1695604070000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-0266-9_18"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"ISBN":["9781071602652","9781071602669"],"references-count":48,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0266-9_18","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"14 January 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}