{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:07:03Z","timestamp":1791266823486,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":23,"publisher":"Springer US","isbn-type":[{"value":"9781071602935","type":"print"},{"value":"9781071602942","type":"electronic"}],"license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-0716-0294-2_46","type":"book-chapter","created":{"date-parts":[[2020,3,27]],"date-time":"2020-03-27T16:02:50Z","timestamp":1585324970000},"page":"781-789","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Vitro Drug Efficacy Testing Against Trypanosoma brucei"],"prefix":"10.1007","author":[{"given":"Marcel","family":"Kaiser","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"M\u00e4ser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,3,28]]},"reference":[{"key":"46_CR1","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/j.coph.2012.05.001","volume":"12","author":"P M\u00e4ser","year":"2012","unstructured":"M\u00e4ser P, Wittlin S, Rottmann M et al (2012) Antiparasitic agents: new drugs on the horizon. Curr Opin Pharmacol 12:562\u2013566","journal-title":"Curr Opin Pharmacol"},{"key":"46_CR2","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/S0140-6736(17)32758-7","volume":"391","author":"V Mesu","year":"2018","unstructured":"Mesu V, Kalonji WM, Bardonneau C et al (2018) Oral fexinidazole for late-stage African Trypanosoma brucei gambiense trypanosomiasis: a pivotal multicentre, randomised, non-inferiority trial. Lancet 391:144\u2013154","journal-title":"Lancet"},{"key":"46_CR3","doi-asserted-by":"publisher","first-page":"e923","DOI":"10.1371\/journal.pntd.0000923","volume":"4","author":"E Torreele","year":"2011","unstructured":"Torreele E, Bourdin Trunz B, Tweats D et al (2011) Fexinidazole\u2014a new oral nitroimidazole drug candidate entering clinical development for the treatment of sleeping sickness. PLoS Negl Trop Dis 4:e923","journal-title":"PLoS Negl Trop Dis"},{"key":"46_CR4","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/S0001-706X(97)00079-X","volume":"68","author":"B R\u00e4z","year":"1997","unstructured":"R\u00e4z B, Iten M, Grether-B\u00fchler Y et al (1997) The Alamar Blue assay to determine drug sensitivity of African trypanosomes in vitro. Acta Trop 68:139\u2013147","journal-title":"Acta Trop"},{"key":"46_CR5","doi-asserted-by":"publisher","first-page":"5602","DOI":"10.1128\/AAC.00246-11","volume":"55","author":"M Kaiser","year":"2011","unstructured":"Kaiser M, Bray MA, Cal M et al (2011) Antitrypanosomal activity of fexinidazole, a new oral nitroimidazole drug candidate for treatment of sleeping sickness. Antimicrob Agents Chemother 55:5602\u20135608","journal-title":"Antimicrob Agents Chemother"},{"key":"46_CR6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001668","volume":"6","author":"T Wenzler","year":"2012","unstructured":"Wenzler T, Steinhuber A, Wittlin S et al (2012) Isothermal microcalorimetry, a new tool to monitor drug action against Trypanosoma brucei and Plasmodium falciparum. PLoS Negl Trop Dis 6:e1668","journal-title":"PLoS Negl Trop Dis"},{"key":"46_CR7","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1002\/j.1460-2075.1985.tb03772.x","volume":"4","author":"T Baltz","year":"1985","unstructured":"Baltz T, Baltz D, Giroud C et al (1985) Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. EMBO J 4:1273\u20131277","journal-title":"EMBO J"},{"key":"46_CR8","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/0001-706X(89)90048-X","volume":"46","author":"R Brun","year":"1989","unstructured":"Brun R, Kunz C (1989) In vitro drug sensitivity test for Trypanosoma brucei subgroup bloodstream trypomastigotes. Acta Trop Basel 46:361\u2013368","journal-title":"Acta Trop Basel"},{"key":"46_CR9","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/0169-4758(94)90402-2","volume":"10","author":"H Hirumi","year":"1994","unstructured":"Hirumi H, Hirumi K (1994) Axenic culture of african trypanosome bloodstream forms. Parasitol Today 10:80\u201384","journal-title":"Parasitol Today"},{"key":"46_CR10","doi-asserted-by":"publisher","first-page":"985","DOI":"10.2307\/3282883","volume":"75","author":"H Hirumi","year":"1989","unstructured":"Hirumi H, Hirumi K (1989) Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J Parasitol 75:985\u2013989","journal-title":"J Parasitol"},{"key":"46_CR11","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/0001-706X(89)90049-1","volume":"46","author":"R Brun","year":"1989","unstructured":"Brun R, Baeriswyl S, Kunz C (1989) In vitro drug sensitivity of Trypanosoma gambiense isolates. Acta Trop Basel 46:369\u2013376","journal-title":"Acta Trop Basel"},{"key":"46_CR12","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1046\/j.1365-3156.2001.00775.x","volume":"6","author":"R Brun","year":"2001","unstructured":"Brun R, Schumacher R, Schmid C et al (2001) The phenomenon of treatment failures in Human African Trypanosomiasis. Tropical Med Int Health 6:906\u2013914","journal-title":"Tropical Med Int Health"},{"key":"46_CR13","first-page":"134","volume":"32","author":"P Felgner","year":"1981","unstructured":"Felgner P, Brinkmann U, Zillmann U et al (1981) Epidemiological studies on the animal reservoir of gambiense sleeping sickness. Part II. Parasitological and immunodiagnostic examination of the human population. Tropenmed Parasitol 32:134\u2013140","journal-title":"Tropenmed Parasitol"},{"key":"46_CR14","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.actatropica.2007.07.007","volume":"104","author":"N Maina","year":"2007","unstructured":"Maina N, Maina KJ, M\u00e4ser P et al (2007) Genotypic and phenotypic characterization of Trypanosoma brucei gambiense isolates from Ibba, South Sudan, an area of high melarsoprol treatment failure rate. Acta Trop 104:84\u201390","journal-title":"Acta Trop"},{"key":"46_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1574-6968.2009.01819.x","volume":"303","author":"O Braissant","year":"2010","unstructured":"Braissant O, Wirz D, Gopfert B et al (2010) Use of isothermal microcalorimetry to monitor microbial activities. FEMS Microbiol Lett 303:1\u20138","journal-title":"FEMS Microbiol Lett"},{"key":"46_CR16","volume-title":"Numerical ecology","author":"P Legendre","year":"2012","unstructured":"Legendre P, Legendre LFJ (2012) Numerical ecology. Elsevier, Amsterdam"},{"key":"46_CR17","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/0166-6851(92)90224-8","volume":"50","author":"M Duszenko","year":"1992","unstructured":"Duszenko M, Muhlstadt K, Broder A (1992) Cysteine is an essential growth factor of Trypanosoma brucei bloodstream forms. Mol Biochem Parasitol 50:269\u2013273","journal-title":"Mol Biochem Parasitol"},{"key":"46_CR18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002587","volume":"7","author":"M Mazet","year":"2013","unstructured":"Mazet M, Morand P, Biran M et al (2013) Revisiting the central metabolism of the bloodstream forms of Trypanosoma brucei: production of acetate in the mitochondrion is essential for parasite viability. PLoS Negl Trop Dis 7:e2587","journal-title":"PLoS Negl Trop Dis"},{"key":"46_CR19","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1124\/mol.104.010298","volume":"68","author":"F Geiser","year":"2005","unstructured":"Geiser F, Luscher A, de Koning HP et al (2005) Molecular pharmacology of adenosine transport in Trypanosoma brucei: P1\/P2 revisited. Mol Pharmacol 68:589\u2013595","journal-title":"Mol Pharmacol"},{"key":"46_CR20","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1177\/1087057115569155","volume":"20","author":"M Kaiser","year":"2015","unstructured":"Kaiser M, Maes L, Tadoori LP et al (2015) Repurposing of the open access malaria box for kinetoplastid diseases identifies novel active scaffolds against trypanosomatids. J Biomol Screen 20:634\u2013645","journal-title":"J Biomol Screen"},{"key":"46_CR21","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1016\/j.tca.2012.12.005","volume":"555","author":"O Braissant","year":"2013","unstructured":"Braissant O, Bonkat G, Wirz D et al (2013) Microbial growth and isothermal microcalorimetry: growth models and their application to microcalorimetric data. Thermochim Acta 555:64\u201371","journal-title":"Thermochim Acta"},{"key":"46_CR22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v033.i07","volume":"33","author":"M Kahm","year":"2010","unstructured":"Kahm M, Hasenbrink G, Lichtenberg-Frate H et al (2010) Grofit: fitting biological growth curves with R. J Stat Software 33:1\u201321","journal-title":"J Stat Software"},{"key":"46_CR23","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.ijpddr.2018.03.003","volume":"8","author":"M Gysin","year":"2018","unstructured":"Gysin M, Braissant O, Gillingwater K et al (2018) Isothermal microcalorimetry \u2013 a quantitative method to monitor Trypanosoma congolense growth and growth inhibition by trypanocidal drugs in real time. Int J Parasitol Drugs Drug Resist 8:159\u2013164","journal-title":"Int J Parasitol Drugs Drug Resist"}],"container-title":["Methods in Molecular Biology","Trypanosomatids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0294-2_46","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T20:58:01Z","timestamp":1757710681000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-0294-2_46"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"ISBN":["9781071602935","9781071602942"],"references-count":23,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0294-2_46","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"28 March 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}