{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T13:42:11Z","timestamp":1742996531696,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":32,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071608050"},{"type":"electronic","value":"9781071608067"}],"license":[{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021]]},"DOI":"10.1007\/978-1-0716-0806-7_15","type":"book-chapter","created":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T15:04:57Z","timestamp":1599923097000},"page":"197-211","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer"],"prefix":"10.1007","author":[{"given":"En-Hsin","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,13]]},"reference":[{"key":"15_CR1","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1039\/b808893d","volume":"4","author":"H Bayley","year":"2008","unstructured":"Bayley H, Cronin B, Heron A et al (2008) Droplet interface bilayers. Mol Biosyst 4:1191\u20131208. \nhttps:\/\/doi.org\/10.1039\/b808893d","journal-title":"Mol Biosyst"},{"key":"15_CR2","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1038\/nprot.2013.061","volume":"8","author":"S Leptihn","year":"2013","unstructured":"Leptihn S, Castell OK, Cronin B et al (2013) Constructing droplet interface bilayers from the contact of aqueous droplets in oil. Nat Protoc 8:1048\u20131057. \nhttps:\/\/doi.org\/10.1038\/nprot.2013.061","journal-title":"Nat Protoc"},{"key":"15_CR3","doi-asserted-by":"publisher","first-page":"12369","DOI":"10.1021\/acs.analchem.7b03421","volume":"89","author":"X Li","year":"2017","unstructured":"Li X, Huang J, Holden MA, Chen M (2017) Peptide-mediated membrane transport of macromolecular cargo driven by membrane asymmetry. Anal Chem 89:12369\u201312374. \nhttps:\/\/doi.org\/10.1021\/acs.analchem.7b03421","journal-title":"Anal Chem"},{"key":"15_CR4","doi-asserted-by":"publisher","first-page":"15818","DOI":"10.1021\/ja2046342","volume":"133","author":"J Huang","year":"2011","unstructured":"Huang J, Lein M, Gunderson C, Holden MA (2011) Direct quantitation of peptide-mediated protein transport across a droplet-interface bilayer. J Am Chem Soc 133:15818\u201315821. \nhttps:\/\/doi.org\/10.1021\/ja2046342","journal-title":"J Am Chem Soc"},{"key":"15_CR5","doi-asserted-by":"publisher","first-page":"16577","DOI":"10.1073\/pnas.1113074108","volume":"108","author":"A Fischer","year":"2011","unstructured":"Fischer A, Holden MA, Pentelute BL, Collier RJ (2011) Ultrasensitive detection of protein translocated through toxin pores in droplet-interface bilayers. Proc Natl Acad Sci U S A 108:16577\u201316581. \nhttps:\/\/doi.org\/10.1073\/pnas.1113074108","journal-title":"Proc Natl Acad Sci U S A"},{"key":"15_CR6","doi-asserted-by":"publisher","first-page":"8169","DOI":"10.1021\/ac0613479","volume":"78","author":"K Funakoshi","year":"2006","unstructured":"Funakoshi K, Suzuki H, Takeuchi S (2006) Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis. Anal Chem 78:8169\u20138174. \nhttps:\/\/doi.org\/10.1021\/ac0613479","journal-title":"Anal Chem"},{"key":"15_CR7","doi-asserted-by":"publisher","first-page":"5878","DOI":"10.1021\/ja802089s","volume":"130","author":"WL Hwang","year":"2008","unstructured":"Hwang WL, Chen M, Cronin B et al (2008) Asymmetric droplet interface bilayers. J Am Chem Soc 130:5878\u20135879. \nhttps:\/\/doi.org\/10.1021\/ja802089s","journal-title":"J Am Chem Soc"},{"key":"15_CR8","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1038\/nnano.2009.121","volume":"4","author":"G Maglia","year":"2009","unstructured":"Maglia G, Heron AJ, Hwang WL et al (2009) Droplet networks with incorporated protein diodes show collective properties. Nat Nanotechnol 4:437\u2013440. \nhttps:\/\/doi.org\/10.1038\/nnano.2009.121","journal-title":"Nat Nanotechnol"},{"key":"15_CR9","doi-asserted-by":"publisher","first-page":"8650","DOI":"10.1021\/ja072292a","volume":"129","author":"MA Holden","year":"2007","unstructured":"Holden MA, Needham D, Bayley H (2007) Functional bionetworks from nanoliter water droplets. J Am Chem Soc 129:8650\u20138655. \nhttps:\/\/doi.org\/10.1021\/ja072292a","journal-title":"J Am Chem Soc"},{"key":"15_CR10","doi-asserted-by":"publisher","first-page":"2242","DOI":"10.1007\/s10439-011-0323-4","volume":"39","author":"C Shao","year":"2011","unstructured":"Shao C, Sun B, Colombini M, DeVoe DL (2011) Rapid microfluidic perfusion enabling kinetic studies of lipid ion channels in a bilayer lipid membrane chip. Ann Biomed Eng 39:2242\u20132251. \nhttps:\/\/doi.org\/10.1007\/s10439-011-0323-4","journal-title":"Ann Biomed Eng"},{"key":"15_CR11","doi-asserted-by":"publisher","first-page":"1476","DOI":"10.1039\/c3lc41359d","volume":"13","author":"Y Tsuji","year":"2013","unstructured":"Tsuji Y, Kawano R, Osaki T et al (2013) Droplet-based lipid bilayer system integrated with microfluidic channels for solution exchange. Lab Chip 13:1476. \nhttps:\/\/doi.org\/10.1039\/c3lc41359d","journal-title":"Lab Chip"},{"key":"15_CR12","doi-asserted-by":"publisher","first-page":"2749","DOI":"10.1039\/c3lc41323c","volume":"13","author":"M Lein","year":"2013","unstructured":"Lein M, Huang J, Holden MA (2013) Robust reagent addition and perfusion strategies for droplet-interface bilayers. Lab Chip 13:2749. \nhttps:\/\/doi.org\/10.1039\/c3lc41323c","journal-title":"Lab Chip"},{"key":"15_CR13","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1039\/b405967k","volume":"4","author":"H Suzuki","year":"2004","unstructured":"Suzuki H, Tabata K, Kato-Yamada Y et al (2004) Planar lipid bilayer reconstitution with a micro-fluidic system. Lab Chip 4:502\u2013505. \nhttps:\/\/doi.org\/10.1039\/b405967k","journal-title":"Lab Chip"},{"key":"15_CR14","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1016\/j.bios.2006.04.013","volume":"22","author":"H Suzuki","year":"2007","unstructured":"Suzuki H, Tabata KV, Noji H, Takeuchi S (2007) Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip. Biosens Bioelectron 22:1111\u20131115. \nhttps:\/\/doi.org\/10.1016\/j.bios.2006.04.013","journal-title":"Biosens Bioelectron"},{"key":"15_CR15","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1146\/annurev.biochem.75.103004.142728","volume":"76","author":"JAT Young","year":"2007","unstructured":"Young JAT, Collier RJ (2007) Anthrax toxin: receptor binding, internalization, pore formation, and translocation. Annu Rev Biochem 76:243\u2013265. \nhttps:\/\/doi.org\/10.1146\/annurev.biochem.75.103004.142728","journal-title":"Annu Rev Biochem"},{"key":"15_CR16","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1146\/annurev.cellbio.19.111301.140655","volume":"19","author":"RJ Collier","year":"2003","unstructured":"Collier RJ, Young JAT (2003) Anthrax toxin. Annu Rev Cell Dev Biol 19:45\u201370. \nhttps:\/\/doi.org\/10.1146\/annurev.cellbio.19.111301.140655","journal-title":"Annu Rev Cell Dev Biol"},{"key":"15_CR17","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1002\/pro.2052","volume":"21","author":"GK Feld","year":"2012","unstructured":"Feld GK, Brown MJ, Krantz BA (2012) Ratcheting up protein translocation with anthrax toxin. Protein Sci 21:606\u2013624. \nhttps:\/\/doi.org\/10.1002\/pro.2052","journal-title":"Protein Sci"},{"key":"15_CR18","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/j.mam.2009.06.003","volume":"30","author":"RJ Collier","year":"2009","unstructured":"Collier RJ (2009) Membrane translocation by anthrax toxin. Mol Asp Med 30:413\u2013422. \nhttps:\/\/doi.org\/10.1016\/j.mam.2009.06.003","journal-title":"Mol Asp Med"},{"key":"15_CR19","doi-asserted-by":"publisher","first-page":"6335","DOI":"10.1021\/bi400705n","volume":"52","author":"S Naik","year":"2013","unstructured":"Naik S, Brock S, Akkaladevi N et al (2013) Monitoring the kinetics of the pH-driven transition of the anthrax toxin prepore to the pore by biolayer interferometry and surface plasmon resonance. Biochemistry 52:6335\u20136347. \nhttps:\/\/doi.org\/10.1021\/bi400705n","journal-title":"Biochemistry"},{"key":"15_CR20","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1038\/n35101998","volume":"414","author":"AD Pannifer","year":"2001","unstructured":"Pannifer AD, Wong TY, Schwarzenbacher R et al (2001) Crystal structure of the anthrax lethal factor. Nature 414:229\u2013233. \nhttps:\/\/doi.org\/10.1038\/n35101998","journal-title":"Nature"},{"key":"15_CR21","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1126\/science.280.5364.734","volume":"280","author":"NS Duesbery","year":"1998","unstructured":"Duesbery NS, Webb CP, Leppla SH et al (1998) Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 280:734\u2013737","journal-title":"Science"},{"key":"15_CR22","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1016\/j.jmb.2005.11.030","volume":"355","author":"BA Krantz","year":"2006","unstructured":"Krantz BA, Finkelstein A, Collier RJ (2006) Protein translocation through the anthrax toxin transmembrane pore is driven by a proton gradient. J Mol Biol 355:968\u2013979. \nhttps:\/\/doi.org\/10.1016\/j.jmb.2005.11.030","journal-title":"J Mol Biol"},{"key":"15_CR23","doi-asserted-by":"publisher","first-page":"23189","DOI":"10.1074\/jbc.M111.231167","volume":"286","author":"MJ Brown","year":"2011","unstructured":"Brown MJ, Thoren KL, Krantz BA (2011) Charge requirements for proton gradient-driven translocation of anthrax toxin. J Biol Chem 286:23189\u201323199. \nhttps:\/\/doi.org\/10.1074\/jbc.M111.231167","journal-title":"J Biol Chem"},{"key":"15_CR24","doi-asserted-by":"publisher","first-page":"43753","DOI":"10.1074\/jbc.M112.419598","volume":"287","author":"SL Wynia-Smith","year":"2012","unstructured":"Wynia-Smith SL, Brown MJ, Chirichella G et al (2012) Electrostatic ratchet in the protective antigen channel promotes anthrax toxin translocation. J Biol Chem 287:43753\u201343764. \nhttps:\/\/doi.org\/10.1074\/jbc.M112.419598","journal-title":"J Biol Chem"},{"key":"15_CR25","doi-asserted-by":"publisher","first-page":"3598","DOI":"10.1016\/j.jmb.2015.09.007","volume":"427","author":"JM Colby","year":"2015","unstructured":"Colby JM, Krantz BA (2015) Peptide probes reveal a hydrophobic steric ratchet in the anthrax toxin protective antigen translocase. J Mol Biol 427:3598\u20133606. \nhttps:\/\/doi.org\/10.1016\/j.jmb.2015.09.007","journal-title":"J Mol Biol"},{"key":"15_CR26","doi-asserted-by":"publisher","first-page":"9611","DOI":"10.1073\/pnas.1600624113","volume":"113","author":"D Das","year":"2016","unstructured":"Das D, Krantz BA (2016) Peptide- and proton-driven allosteric clamps catalyze anthrax toxin translocation across membranes. Proc Natl Acad Sci 113:9611\u20139616. \nhttps:\/\/doi.org\/10.1073\/pnas.1600624113","journal-title":"Proc Natl Acad Sci"},{"key":"15_CR27","doi-asserted-by":"publisher","first-page":"15737","DOI":"10.1021\/bi981436i","volume":"37","author":"J Wesche","year":"1998","unstructured":"Wesche J, Elliott JL, Falnes PO et al (1998) Characterization of membrane translocation by anthrax protective antigen. Biochemistry 37:15737\u201315746. \nhttps:\/\/doi.org\/10.1021\/bi981436i","journal-title":"Biochemistry"},{"key":"15_CR28","doi-asserted-by":"publisher","first-page":"7049","DOI":"10.1073\/pnas.062160399","volume":"99","author":"K Cunningham","year":"2002","unstructured":"Cunningham K, Lacy DB, Mogridge J, Collier RJ (2002) Mapping the lethal factor and edema factor binding sites on oligomeric anthrax protective antigen. Proc Natl Acad Sci U S A 99:7049\u20137053. \nhttps:\/\/doi.org\/10.1073\/pnas.062160399","journal-title":"Proc Natl Acad Sci U S A"},{"key":"15_CR29","doi-asserted-by":"publisher","first-page":"3842","DOI":"10.1529\/biophysj.104.050864","volume":"87","author":"S Zhang","year":"2004","unstructured":"Zhang S, Udho E, Wu Z et al (2004) Protein translocation through anthrax toxin channels formed in planar lipid bilayers. Biophys J 87:3842\u20133849. \nhttps:\/\/doi.org\/10.1529\/biophysj.104.050864","journal-title":"Biophys J"},{"key":"15_CR30","doi-asserted-by":"publisher","first-page":"14335","DOI":"10.1021\/la203081v","volume":"27","author":"LCM Gross","year":"2011","unstructured":"Gross LCM, Heron AJ, Baca SC, Wallace MI (2011) Determining membrane capacitance by dynamic control of droplet interface bilayer area. Langmuir 27:14335\u201314342. \nhttps:\/\/doi.org\/10.1021\/la203081v","journal-title":"Langmuir"},{"key":"15_CR31","doi-asserted-by":"publisher","first-page":"16756","DOI":"10.1073\/pnas.0405754101","volume":"101","author":"S Zhang","year":"2004","unstructured":"Zhang S, Finkelstein A, Collier RJ (2004) Evidence that translocation of anthrax toxin\u2019s lethal factor is initiated by entry of its N terminus into the protective antigen channel. Proc Natl Acad Sci U S A 101:16756\u201316761. \nhttps:\/\/doi.org\/10.1073\/pnas.0405754101","journal-title":"Proc Natl Acad Sci U S A"},{"key":"15_CR32","unstructured":"Lee E-H (2017) Mechanistic studies of proton gradient-driven protein translocation by droplet-interface bilayer techniques. Dissertation, University of Massachusetts Amherst"}],"container-title":["Methods in Molecular Biology","Nanopore Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0806-7_15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,12]],"date-time":"2020-09-12T15:05:34Z","timestamp":1599923134000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-0806-7_15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,13]]},"ISBN":["9781071608050","9781071608067"],"references-count":32,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0806-7_15","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2020,9,13]]},"assertion":[{"value":"13 September 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}