{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:06:48Z","timestamp":1790222808068,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":63,"publisher":"Springer US","isbn-type":[{"value":"9781071608135","type":"print"},{"value":"9781071608142","type":"electronic"}],"license":[{"start":{"date-parts":[[2020,8,9]],"date-time":"2020-08-09T00:00:00Z","timestamp":1596931200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,8,9]],"date-time":"2020-08-09T00:00:00Z","timestamp":1596931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021]]},"DOI":"10.1007\/978-1-0716-0814-2_9","type":"book-chapter","created":{"date-parts":[[2020,8,8]],"date-time":"2020-08-08T11:03:23Z","timestamp":1596884603000},"page":"147-186","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas\/CD95 Location in Rafts During Apoptosis Triggering"],"prefix":"10.1007","author":[{"given":"Consuelo","family":"Gajate","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faustino","family":"Mollinedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,9]]},"reference":[{"key":"9_CR1","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1038\/42408","volume":"387","author":"K Simons","year":"1997","unstructured":"Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387:569\u2013572","journal-title":"Nature"},{"key":"9_CR2","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1172\/JCI0216390","volume":"110","author":"K Simons","year":"2002","unstructured":"Simons K, Ehehalt R (2002) Cholesterol, lipid rafts, and disease. J Clin Invest 110:597\u2013603","journal-title":"J Clin Invest"},{"key":"9_CR3","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1046\/j.0014-2956.2001.02715.x","volume":"269","author":"LD Zajchowski","year":"2002","unstructured":"Zajchowski LD, Robbins SM (2002) Lipid rafts and little caves. Compartmentalized signalling in membrane microdomains. Eur J Biochem 269:737\u2013752","journal-title":"Eur J Biochem"},{"key":"9_CR4","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.drup.2006.04.002","volume":"9","author":"F Mollinedo","year":"2006","unstructured":"Mollinedo F, Gajate C (2006) Fas\/CD95 death receptor and lipid rafts: new targets for apoptosis-directed cancer therapy. Drug Resist Updat 9:51\u201373","journal-title":"Drug Resist Updat"},{"key":"9_CR5","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1194\/jlr.E600002-JLR200","volume":"47","author":"LJ Pike","year":"2006","unstructured":"Pike LJ (2006) Rafts defined: a report on the keystone symposium on lipid rafts and cell function. J Lipid Res 47:1597\u20131598","journal-title":"J Lipid Res"},{"issue":"Suppl","key":"9_CR6","doi-asserted-by":"publisher","first-page":"S323","DOI":"10.1194\/jlr.R800040-JLR200","volume":"50","author":"LJ Pike","year":"2009","unstructured":"Pike LJ (2009) The challenge of lipid rafts. J Lipid Res 50(Suppl):S323\u2013S328","journal-title":"J Lipid Res"},{"key":"9_CR7","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.jbior.2014.10.003","volume":"57","author":"F Mollinedo","year":"2015","unstructured":"Mollinedo F, Gajate C (2015) Lipid rafts as major platforms for signaling regulation in cancer. Adv Biol Regul 57:130\u2013146","journal-title":"Adv Biol Regul"},{"key":"9_CR8","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1146\/annurev.cellbio.14.1.111","volume":"14","author":"DA Brown","year":"1998","unstructured":"Brown DA, London E (1998) Functions of lipid rafts in biological membranes. Annu Rev Cell Dev Biol 14:111\u2013136","journal-title":"Annu Rev Cell Dev Biol"},{"key":"9_CR9","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1038\/35036052","volume":"1","author":"K Simons","year":"2000","unstructured":"Simons K, Toomre D (2000) Lipid rafts and signal transduction. Nat Rev Mol Cell Biol 1:31\u201339","journal-title":"Nat Rev Mol Cell Biol"},{"key":"9_CR10","doi-asserted-by":"publisher","first-page":"17221","DOI":"10.1074\/jbc.R000005200","volume":"275","author":"DA Brown","year":"2000","unstructured":"Brown DA, London E (2000) Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J Biol Chem 275:17221\u201317224","journal-title":"J Biol Chem"},{"key":"9_CR11","doi-asserted-by":"publisher","first-page":"1779","DOI":"10.1038\/sj.onc.1210816","volume":"27","author":"T Nieto-Miguel","year":"2008","unstructured":"Nieto-Miguel T, Gajate C, Gonzalez-Camacho F, Mollinedo F (2008) Proapoptotic role of Hsp90 by its interaction with c-Jun N-terminal kinase in lipid rafts in edelfosine-mediated antileukemic therapy. Oncogene 27:1779\u20131787","journal-title":"Oncogene"},{"key":"9_CR12","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.pharmthera.2016.06.003","volume":"165","author":"PA Jaffres","year":"2016","unstructured":"Jaffres PA, Gajate C, Bouchet AM, Couthon-Gourves H, Chantome A, Potier-Cartereau M, Besson P, Bougnoux P, Mollinedo F, Vandier C (2016) Alkyl ether lipids, ion channels and lipid raft reorganization in cancer therapy. Pharmacol Ther 165:114\u2013131","journal-title":"Pharmacol Ther"},{"key":"9_CR13","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/S0304-4157(00)00007-1","volume":"1508","author":"E London","year":"2000","unstructured":"London E, Brown DA (2000) Insolubility of lipids in triton X-100: physical origin and relationship to sphingolipid\/cholesterol membrane domains (rafts). Biochim Biophys Acta 1508:182\u2013195","journal-title":"Biochim Biophys Acta"},{"key":"9_CR14","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1194\/jlr.R200021-JLR200","volume":"44","author":"LJ Pike","year":"2003","unstructured":"Pike LJ (2003) Lipid rafts: bringing order to chaos. J Lipid Res 44:655\u2013667","journal-title":"J Lipid Res"},{"key":"9_CR15","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1111\/j.1600-0854.2003.00163.x","volume":"5","author":"T Harder","year":"2004","unstructured":"Harder T, Engelhardt KR (2004) Membrane domains in lymphocytes\u2014from lipid rafts to protein scaffolds. Traffic 5:265\u2013275","journal-title":"Traffic"},{"key":"9_CR16","doi-asserted-by":"publisher","first-page":"6238","DOI":"10.1158\/0008-5472.CAN-07-0288","volume":"67","author":"RM Adam","year":"2007","unstructured":"Adam RM, Mukhopadhyay NK, Kim J, Di Vizio D, Cinar B, Boucher K, Solomon KR, Freeman MR (2007) Cholesterol sensitivity of endogenous and myristoylated Akt. Cancer Res 67:6238\u20136246","journal-title":"Cancer Res"},{"key":"9_CR17","doi-asserted-by":"publisher","first-page":"14509","DOI":"10.1073\/pnas.1019386108","volume":"108","author":"X Gao","year":"2011","unstructured":"Gao X, Lowry PR, Zhou X, Depry C, Wei Z, Wong GW, Zhang J (2011) PI3K\/Akt signaling requires spatial compartmentalization in plasma membrane microdomains. Proc Natl Acad Sci U S A 108:14509\u201314514","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR18","doi-asserted-by":"publisher","first-page":"32","DOI":"10.4161\/cib.2.1.7420","volume":"2","author":"X Gao","year":"2009","unstructured":"Gao X, Zhang J (2009) Akt signaling dynamics in plasma membrane microdomains visualized by FRET-based reporters. Commun Integr Biol 2:32\u201334","journal-title":"Commun Integr Biol"},{"key":"9_CR19","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2013.15","volume":"3","author":"M Reis-Sobreiro","year":"2013","unstructured":"Reis-Sobreiro M, Roue G, Moros A, Gajate C, de la Iglesia-Vicente J, Colomer D, Mollinedo F (2013) Lipid raft-mediated Akt signaling as a therapeutic target in mantle cell lymphoma. Blood Cancer J 3:e118","journal-title":"Blood Cancer J"},{"key":"9_CR20","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1084\/jem.20040213","volume":"200","author":"C Gajate","year":"2004","unstructured":"Gajate C, Del Canto-Janez E, Acuna AU, Amat-Guerri F, Geijo E, Santos-Beneit AM, Veldman RJ, Mollinedo F (2004) Intracellular triggering of Fas aggregation and recruitment of apoptotic molecules into Fas-enriched rafts in selective tumor cell apoptosis. J Exp Med 200:353\u2013365","journal-title":"J Exp Med"},{"key":"9_CR21","doi-asserted-by":"publisher","first-page":"3860","DOI":"10.1182\/blood.V98.13.3860","volume":"98","author":"C Gajate","year":"2001","unstructured":"Gajate C, Mollinedo F (2001) The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas\/CD95 into membrane rafts in human leukemic cells. Blood 98:3860\u20133863","journal-title":"Blood"},{"key":"9_CR22","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1182\/blood-2006-04-016824","volume":"109","author":"C Gajate","year":"2007","unstructured":"Gajate C, Mollinedo F (2007) Edelfosine and perifosine induce selective apoptosis in multiple myeloma by recruitment of death receptors and downstream signaling molecules into lipid rafts. Blood 109:711\u2013719","journal-title":"Blood"},{"key":"9_CR23","doi-asserted-by":"publisher","first-page":"3748","DOI":"10.1038\/onc.2010.131","volume":"29","author":"F Mollinedo","year":"2010","unstructured":"Mollinedo F, de la Iglesia-Vicente J, Gajate C, Estella-Hermoso de Mendoza A, Villa-Pulgarin JA, Campanero MA, Blanco-Prieto MJ (2010) Lipid raft-targeted therapy in multiple myeloma. Oncogene 29:3748\u20133757","journal-title":"Oncogene"},{"key":"9_CR24","doi-asserted-by":"publisher","first-page":"2046","DOI":"10.1158\/1078-0432.CCR-09-2456","volume":"16","author":"F Mollinedo","year":"2010","unstructured":"Mollinedo F, de la Iglesia-Vicente J, Gajate C, Estella-Hermoso de Mendoza A, Villa-Pulgarin JA, de Frias M, Roue G, Gil J, Colomer D, Campanero MA, Blanco-Prieto MJ (2010) In vitro and In vivo selective antitumor activity of Edelfosine against mantle cell lymphoma and chronic lymphocytic leukemia involving lipid rafts. Clin Cancer Res 16:2046\u20132054","journal-title":"Clin Cancer Res"},{"key":"9_CR25","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1189\/jlb.2MR0215-055R","volume":"98","author":"C Gajate","year":"2015","unstructured":"Gajate C, Mollinedo F (2015) Lipid raft-mediated Fas\/CD95 apoptotic signaling in leukemic cells and normal leukocytes and therapeutic implications. J Leukoc Biol 98:739\u2013759","journal-title":"J Leukoc Biol"},{"key":"9_CR26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005044","volume":"4","author":"C Gajate","year":"2009","unstructured":"Gajate C, Gonzalez-Camacho F, Mollinedo F (2009) Involvement of raft aggregates enriched in Fas\/CD95 death-inducing signaling complex in the antileukemic action of edelfosine in Jurkat cells. PLoS One 4:e5044","journal-title":"PLoS One"},{"key":"9_CR27","doi-asserted-by":"publisher","first-page":"3221","DOI":"10.1038\/onc.2009.183","volume":"28","author":"M Reis-Sobreiro","year":"2009","unstructured":"Reis-Sobreiro M, Gajate C, Mollinedo F (2009) Involvement of mitochondria and recruitment of Fas\/CD95 signaling in lipid rafts in resveratrol-mediated antimyeloma and antileukemia actions. Oncogene 28:3221\u20133234","journal-title":"Oncogene"},{"key":"9_CR28","doi-asserted-by":"publisher","first-page":"11641","DOI":"10.1074\/jbc.M411781200","volume":"280","author":"C Gajate","year":"2005","unstructured":"Gajate C, Mollinedo F (2005) Cytoskeleton-mediated death receptor and ligand concentration in lipid rafts forms apoptosis-promoting clusters in cancer chemotherapy. J Biol Chem 280:11641\u201311647","journal-title":"J Biol Chem"},{"key":"9_CR29","doi-asserted-by":"publisher","first-page":"780","DOI":"10.1016\/j.bbrc.2009.01.147","volume":"380","author":"C Gajate","year":"2009","unstructured":"Gajate C, Gonzalez-Camacho F, Mollinedo F (2009) Lipid raft connection between extrinsic and intrinsic apoptotic pathways. Biochem Biophys Res Commun 380:780\u2013784","journal-title":"Biochem Biophys Res Commun"},{"key":"9_CR30","doi-asserted-by":"publisher","first-page":"811","DOI":"10.2217\/fon.10.34","volume":"6","author":"F Mollinedo","year":"2010","unstructured":"Mollinedo F, Gajate C (2010) Lipid rafts and clusters of apoptotic signaling molecule-enriched rafts in cancer therapy. Future Oncol 6:811\u2013821","journal-title":"Future Oncol"},{"key":"9_CR31","doi-asserted-by":"publisher","first-page":"491","DOI":"10.2217\/fon.10.22","volume":"6","author":"F Mollinedo","year":"2010","unstructured":"Mollinedo F, Gajate C (2010) Lipid rafts, death receptors and CASMERs: new insights for cancer therapy. Future Oncol 6:491\u2013494","journal-title":"Future Oncol"},{"key":"9_CR32","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1007\/s10495-015-1104-6","volume":"20","author":"C Gajate","year":"2015","unstructured":"Gajate C, Mollinedo F (2015) Lipid rafts and raft-mediated supramolecular entities in the regulation of CD95 death receptor apoptotic signaling. Apoptosis 20:584\u2013606","journal-title":"Apoptosis"},{"key":"9_CR33","doi-asserted-by":"publisher","first-page":"509","DOI":"10.2174\/1871520614666140309222259","volume":"14","author":"C Gajate","year":"2014","unstructured":"Gajate C, Mollinedo F (2014) Lipid rafts, endoplasmic reticulum and mitochondria in the antitumor action of the alkylphospholipid analog edelfosine. Anticancer Agents Med Chem 14:509\u2013527","journal-title":"Anticancer Agents Med Chem"},{"key":"9_CR34","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/S0092-8674(03)00882-1","volume":"115","author":"S Munro","year":"2003","unstructured":"Munro S (2003) Lipid rafts: elusive or illusive? Cell 115:377\u2013388","journal-title":"Cell"},{"key":"9_CR35","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1038\/s41467-017-01328-3","volume":"8","author":"JH Lorent","year":"2017","unstructured":"Lorent JH, Diaz-Rohrer B, Lin X, Spring K, Gorfe AA, Levental KR, Levental I (2017) Structural determinants and functional consequences of protein affinity for membrane rafts. Nat Commun 8:1219","journal-title":"Nat Commun"},{"key":"9_CR36","doi-asserted-by":"publisher","first-page":"2159","DOI":"10.1038\/nprot.2007.294","volume":"2","author":"D Lingwood","year":"2007","unstructured":"Lingwood D, Simons K (2007) Detergent resistance as a tool in membrane research. Nat Protoc 2:2159\u20132165","journal-title":"Nat Protoc"},{"key":"9_CR37","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/978-1-4939-6780-3_13","volume":"1557","author":"C Gajate","year":"2017","unstructured":"Gajate C, Mollinedo F (2017) Isolation of lipid rafts through discontinuous sucrose gradient centrifugation and Fas\/CD95 death receptor localization in raft fractions. Methods Mol Biol 1557:125\u2013138","journal-title":"Methods Mol Biol"},{"key":"9_CR38","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/0092-8674(92)90189-J","volume":"68","author":"DA Brown","year":"1992","unstructured":"Brown DA, Rose JK (1992) Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68:533\u2013544","journal-title":"Cell"},{"key":"9_CR39","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1083\/jcb.200307087","volume":"162","author":"EC Lai","year":"2003","unstructured":"Lai EC (2003) Lipid rafts make for slippery platforms. J Cell Biol 162:365\u2013370","journal-title":"J Cell Biol"},{"key":"9_CR40","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1042\/bj20031672","volume":"378","author":"LJ Pike","year":"2004","unstructured":"Pike LJ (2004) Lipid rafts: heterogeneity on the high seas. Biochem J 378:281\u2013292","journal-title":"Biochem J"},{"key":"9_CR41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1006\/bbrc.1997.7575","volume":"240","author":"DA Brown","year":"1997","unstructured":"Brown DA, London E (1997) Structure of detergent-resistant membrane domains: does phase separation occur in biological membranes? Biochem Biophys Res Commun 240:1\u20137","journal-title":"Biochem Biophys Res Commun"},{"key":"9_CR42","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1074\/jbc.273.2.1150","volume":"273","author":"RJ Schroeder","year":"1998","unstructured":"Schroeder RJ, Ahmed SN, Zhu Y, London E, Brown DA (1998) Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositol-anchored proteins by promoting the formation of detergent-insoluble ordered membrane domains. J Biol Chem 273:1150\u20131157","journal-title":"J Biol Chem"},{"key":"9_CR43","doi-asserted-by":"publisher","first-page":"5795","DOI":"10.1073\/pnas.0631579100","volume":"100","author":"S Schuck","year":"2003","unstructured":"Schuck S, Honsho M, Ekroos K, Shevchenko A, Simons K (2003) Resistance of cell membranes to different detergents. Proc Natl Acad Sci U S A 100:5795\u20135800","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR44","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1002\/eji.1830230409","volume":"23","author":"J Bohuslav","year":"1993","unstructured":"Bohuslav J, Cinek T, Horejsi V (1993) Large, detergent-resistant complexes containing murine antigens Thy-1 and Ly-6 and protein tyrosine kinase p56lck. Eur J Immunol 23:825\u2013831","journal-title":"Eur J Immunol"},{"key":"9_CR45","doi-asserted-by":"publisher","first-page":"6917","DOI":"10.1093\/emboj\/18.24.6917","volume":"18","author":"N Madore","year":"1999","unstructured":"Madore N, Smith KL, Graham CH, Jen A, Brady K, Hall S, Morris R (1999) Functionally different GPI proteins are organized in different domains on the neuronal surface. EMBO J 18:6917\u20136926","journal-title":"EMBO J"},{"key":"9_CR46","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1093\/emboj\/21.8.1899","volume":"21","author":"P Drevot","year":"2002","unstructured":"Drevot P, Langlet C, Guo XJ, Bernard AM, Colard O, Chauvin JP, Lasserre R, He HT (2002) TCR signal initiation machinery is pre-assembled and activated in a subset of membrane rafts. EMBO J 21:1899\u20131908","journal-title":"EMBO J"},{"key":"9_CR47","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1038\/35023524","volume":"2","author":"K Roper","year":"2000","unstructured":"Roper K, Corbeil D, Huttner WB (2000) Retention of prominin in microvilli reveals distinct cholesterol-based lipid micro-domains in the apical plasma membrane. Nat Cell Biol 2:582\u2013592","journal-title":"Nat Cell Biol"},{"key":"9_CR48","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1034\/j.1600-0854.2002.030404.x","volume":"3","author":"W Drobnik","year":"2002","unstructured":"Drobnik W, Borsukova H, Bottcher A, Pfeiffer A, Liebisch G, Schutz GJ, Schindler H, Schmitz G (2002) Apo AI\/ABCA1-dependent and HDL3-mediated lipid efflux from compositionally distinct cholesterol-based microdomains. Traffic 3:268\u2013278","journal-title":"Traffic"},{"key":"9_CR49","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1091\/mbc.e02-08-0528","volume":"14","author":"TA Slimane","year":"2003","unstructured":"Slimane TA, Trugnan G, Van ISC, Hoekstra D (2003) Raft-mediated trafficking of apical resident proteins occurs in both direct and transcytotic pathways in polarized hepatic cells: role of distinct lipid microdomains. Mol Biol Cell 14:611\u2013624","journal-title":"Mol Biol Cell"},{"key":"9_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0014-5793(04)00050-X","volume":"559","author":"LH Chamberlain","year":"2004","unstructured":"Chamberlain LH (2004) Detergents as tools for the purification and classification of lipid rafts. FEBS Lett 559:1\u20135","journal-title":"FEBS Lett"},{"key":"9_CR51","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1042\/bj20031348","volume":"380","author":"G Radeva","year":"2004","unstructured":"Radeva G, Sharom FJ (2004) Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells. Biochem J 380:219\u2013230","journal-title":"Biochem J"},{"key":"9_CR52","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1091\/mbc.10.4.891","volume":"10","author":"S Ilangumaran","year":"1999","unstructured":"Ilangumaran S, Arni S, van Echten-Deckert G, Borisch B, Hoessli DC (1999) Microdomain-dependent regulation of Lck and Fyn protein-tyrosine kinases in T lymphocyte plasma membranes. Mol Biol Cell 10:891\u2013905","journal-title":"Mol Biol Cell"},{"key":"9_CR53","doi-asserted-by":"publisher","first-page":"634","DOI":"10.3109\/09537104.2016.1174203","volume":"27","author":"V Rabani","year":"2016","unstructured":"Rabani V, Davani S, Gambert-Nicot S, Meneveau N, Montange D (2016) Comparative lipidomics and proteomics analysis of platelet lipid rafts using different detergents. Platelets 27:634\u2013641","journal-title":"Platelets"},{"key":"9_CR54","doi-asserted-by":"publisher","first-page":"2693","DOI":"10.1016\/S0006-3495(02)75278-8","volume":"83","author":"H Heerklotz","year":"2002","unstructured":"Heerklotz H (2002) Triton promotes domain formation in lipid raft mixtures. Biophys J 83:2693\u20132701","journal-title":"Biophys J"},{"key":"9_CR55","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1016\/S0022-2836(03)00504-7","volume":"329","author":"H Heerklotz","year":"2003","unstructured":"Heerklotz H, Szadkowska H, Anderson T, Seelig J (2003) The sensitivity of lipid domains to small perturbations demonstrated by the effect of Triton. J Mol Biol 329:793\u2013799","journal-title":"J Mol Biol"},{"key":"9_CR56","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1042\/BJ20090051","volume":"420","author":"M Ingelmo-Torres","year":"2009","unstructured":"Ingelmo-Torres M, Gaus K, Herms A, Gonzalez-Moreno E, Kassan A, Bosch M, Grewal T, Tebar F, Enrich C, Pol A (2009) Triton X-100 promotes a cholesterol-dependent condensation of the plasma membrane. Biochem J 420:373\u2013381","journal-title":"Biochem J"},{"key":"9_CR57","doi-asserted-by":"publisher","first-page":"2264","DOI":"10.1016\/S0022-2275(20)34940-3","volume":"38","author":"AE Christian","year":"1997","unstructured":"Christian AE, Haynes MP, Phillips MC, Rothblat GH (1997) Use of cyclodextrins for manipulating cellular cholesterol content. J Lipid Res 38:2264\u20132272","journal-title":"J Lipid Res"},{"key":"9_CR58","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1016\/j.bpj.2013.09.060","volume":"106","author":"ER Cruz-Chu","year":"2014","unstructured":"Cruz-Chu ER, Malafeev A, Pajarskas T, Pivkin IV, Koumoutsakos P (2014) Structure and response to flow of the glycocalyx layer. Biophys J 106:232\u2013243","journal-title":"Biophys J"},{"key":"9_CR59","doi-asserted-by":"publisher","first-page":"2003","DOI":"10.3390\/biom5032003","volume":"5","author":"K Prydz","year":"2015","unstructured":"Prydz K (2015) Determinants of glycosaminoglycan (GAG) structure. Biomolecules 5:2003\u20132022","journal-title":"Biomolecules"},{"key":"9_CR60","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1016\/0003-2697(78)90784-4","volume":"86","author":"GT James","year":"1978","unstructured":"James GT (1978) Inactivation of the protease inhibitor phenylmethylsulfonyl fluoride in buffers. Anal Biochem 86:574\u2013579","journal-title":"Anal Biochem"},{"key":"9_CR61","doi-asserted-by":"publisher","first-page":"5019","DOI":"10.1021\/bi00438a017","volume":"28","author":"A Schon","year":"1989","unstructured":"Schon A, Freire E (1989) Thermodynamics of intersubunit interactions in cholera toxin upon binding to the oligosaccharide portion of its cell surface receptor, ganglioside GM1. Biochemistry 28:5019\u20135024","journal-title":"Biochemistry"},{"key":"9_CR62","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1083\/jcb.141.4.929","volume":"141","author":"T Harder","year":"1998","unstructured":"Harder T, Scheiffele P, Verkade P, Simons K (1998) Lipid domain structure of the plasma membrane revealed by patching of membrane components. J Cell Biol 141:929\u2013942","journal-title":"J Cell Biol"},{"key":"9_CR63","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1083\/jcb.141.4.917","volume":"141","author":"AA Wolf","year":"1998","unstructured":"Wolf AA, Jobling MG, Wimer-Mackin S, Ferguson-Maltzman M, Madara JL, Holmes RK, Lencer WI (1998) Ganglioside structure dictates signal transduction by cholera toxin and association with caveolae-like membrane domains in polarized epithelia. J Cell Biol 141:917\u2013927","journal-title":"J Cell Biol"}],"container-title":["Methods in Molecular Biology","Lipid Rafts"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0814-2_9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,26]],"date-time":"2023-01-26T16:15:55Z","timestamp":1674749755000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-0814-2_9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,9]]},"ISBN":["9781071608135","9781071608142"],"references-count":63,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0814-2_9","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,9]]},"assertion":[{"value":"9 August 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}