{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:55:53Z","timestamp":1792734953168,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":13,"publisher":"Springer US","isbn-type":[{"value":"9781071608999","type":"print"},{"value":"9781071609002","type":"electronic"}],"license":[{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,1,1]],"date-time":"2020-01-01T00:00:00Z","timestamp":1577836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020]]},"DOI":"10.1007\/978-1-0716-0900-2_10","type":"book-chapter","created":{"date-parts":[[2020,8,24]],"date-time":"2020-08-24T12:03:40Z","timestamp":1598270620000},"page":"119-134","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures"],"prefix":"10.1007","author":[{"given":"Mitra","family":"Gultom","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Laloli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Dijkman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,8,25]]},"reference":[{"key":"10_CR1","doi-asserted-by":"publisher","first-page":"1967","DOI":"10.1056\/NEJMoa030747","volume":"348","author":"C Drosten","year":"2003","unstructured":"Drosten C, G\u00fcnther S, Preiser W et al (2003) Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348:1967\u20131976","journal-title":"N Engl J Med"},{"key":"10_CR2","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1056\/NEJMoa1211721","volume":"367","author":"AM Zaki","year":"2012","unstructured":"Zaki AM, Van Boheemen S, Bestebroer TM et al (2012) Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 367:1814\u20131820","journal-title":"N Engl J Med"},{"key":"10_CR3","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1080\/01652176.1986.9694050","volume":"8","author":"M Pensaert","year":"1986","unstructured":"Pensaert M, Callebaut P, Vergote J (1986) Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet Q 8:257\u2013261","journal-title":"Vet Q"},{"issue":"2","key":"10_CR4","first-page":"125","volume":"24","author":"H Laude","year":"1993","unstructured":"Laude H, Van Reeth K, Pensaert M (1993) Porcine respiratory coronavirus: molecular features and virus-host interactions. Vet Res 24(2):125\u2013150","journal-title":"Vet Res"},{"key":"10_CR5","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/S0007-1935(05)80210-6","volume":"149","author":"MA Clark","year":"1993","unstructured":"Clark MA (1993) Bovine coronavirus. Br Vet J 149:51\u201370","journal-title":"Br Vet J"},{"key":"10_CR6","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.jviromet.2008.05.016","volume":"151","author":"N Decaro","year":"2008","unstructured":"Decaro N, Elia G, Campolo M et al (2008) Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay. J Virol Methods 151:167\u2013171","journal-title":"J Virol Methods"},{"key":"10_CR7","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1136\/vr.130.7.133","volume":"130","author":"DD Addie","year":"1992","unstructured":"Addie DD, Jarrett O (1992) A study of naturally occurring feline coronavirus infections in kittens. Vet Rec 130:133\u2013137","journal-title":"Vet Rec"},{"key":"10_CR8","doi-asserted-by":"publisher","first-page":"1595","DOI":"10.1128\/JVI.79.3.1595-1604.2005","volume":"79","author":"L Vijgen","year":"2005","unstructured":"Vijgen L, Keyaerts E, Mo\u00ebs E et al (2005) Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event. J Virol 79:1595\u20131604","journal-title":"J Virol"},{"key":"10_CR9","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1126\/science.1087139","volume":"302","author":"Y Guan","year":"2003","unstructured":"Guan Y, Zheng BJ, He YQ et al (2003) Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China. Science 302:276\u2013278","journal-title":"Science"},{"key":"10_CR10","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.3201\/eid2008.140663","volume":"20","author":"VS Raj","year":"2014","unstructured":"Raj VS, Farag EABA, Reusken CBEM et al (2014) Isolation of MERS coronavirus from a dromedary camel, Qatar, 2014. Emerg Infect Dis 20:1339","journal-title":"Emerg Infect Dis"},{"key":"10_CR11","unstructured":"Jonsdottir HR, Dijkman R (2015) Characterization of human coronaviruses on well-differentiated human airway epithelial cell cultures. In: Coronaviruses. Springer, New York, pp 73\u201387"},{"key":"10_CR12","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1016\/j.celrep.2018.09.072","volume":"25","author":"C Zhang","year":"2018","unstructured":"Zhang C, Lee HJ, Shrivastava A et al (2018) Long-term in vitro expansion of epithelial stem cells enabled by pharmacological inhibition of PAK1-ROCK-Myosin II and TGF-\u03b2 signaling. Cell Rep 25:598\u2013610","journal-title":"Cell Rep"},{"key":"10_CR13","doi-asserted-by":"publisher","first-page":"7349","DOI":"10.1038\/s41598-018-25799-6","volume":"8","author":"E Eenjes","year":"2018","unstructured":"Eenjes E, Mertens TCJ, Buscop-van Kempen MJ et al (2018) A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture. Sci Rep 8:7349","journal-title":"Sci Rep"}],"container-title":["Methods in Molecular Biology","Coronaviruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0900-2_10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T20:42:29Z","timestamp":1619210549000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-0900-2_10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"ISBN":["9781071608999","9781071609002"],"references-count":13,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0900-2_10","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020]]},"assertion":[{"value":"25 August 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}