{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T08:18:09Z","timestamp":1742977089453,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":25,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071609811"},{"type":"electronic","value":"9781071609828"}],"license":[{"start":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T00:00:00Z","timestamp":1601078400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T00:00:00Z","timestamp":1601078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021]]},"DOI":"10.1007\/978-1-0716-0982-8_4","type":"book-chapter","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T10:02:38Z","timestamp":1601028158000},"page":"41-53","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay"],"prefix":"10.1007","author":[{"given":"Anna Kristina","family":"Witte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Mester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Rossmanith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,9,26]]},"reference":[{"key":"4_CR1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.bdq.2017.11.001","volume":"14","author":"S Bustin","year":"2017","unstructured":"Bustin S, Huggett J (2017) qPCR primer design revisited. Biomol Detect Quantif 14:19\u201328. \nhttps:\/\/doi.org\/10.1016\/j.bdq.2017.11.001","journal-title":"Biomol Detect Quantif"},{"key":"4_CR2","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1016\/j.forsciint.2012.10.003","volume":"223","author":"GS Buzard","year":"2012","unstructured":"Buzard GS, Baker D, Wolcott MJ, Norwood DA, Dauphin LA (2012) Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness. Forensic Sci Int 223:292\u2013297","journal-title":"Forensic Sci Int"},{"key":"4_CR3","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1111\/jam.12937","volume":"119","author":"MSR Fachmann","year":"2015","unstructured":"Fachmann MSR, Josefsen MH, Hoorfar J, Nielsen MT, Loefstroem C (2015) Cost-effective optimization of real-time PCR-based detection of campylobacter and salmonella with inhibitor tolerant DNA polymerases. J Appl Microbiol 119:1391\u20131402","journal-title":"J Appl Microbiol"},{"key":"4_CR4","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.bdq.2018.10.002","volume":"16","author":"AK Witte","year":"2018","unstructured":"Witte AK, Sickha R, Mester P, Fister S, Schoder D, Rossmanith P (2018) Essential role of polymerases for assay performance\u2014impact of polymerase replacement in a well-established assay. Biomol Detect Quantif 16:12\u201320. \nhttps:\/\/doi.org\/10.1016\/j.bdq.2018.10.002","journal-title":"Biomol Detect Quantif"},{"key":"4_CR5","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","volume":"55","author":"SA Bustin","year":"2009","unstructured":"Bustin SA, Benes V, Garson JA et al (2009) The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 55:611\u2013622","journal-title":"Clin Chem"},{"key":"4_CR6","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.tifs.2014.03.008","volume":"37","author":"S Broeders","year":"2014","unstructured":"Broeders S, Huber I, Grohmann L, Berben G, Taverniers I, Mazzara M, Roosens N, Morisset D (2014) Guidelines for validation of qualitative real-time PCR methods. Trends Food Sci Technol 37:115\u2013126","journal-title":"Trends Food Sci Technol"},{"issue":"3","key":"4_CR7","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1002\/jcla.20307","volume":"23","author":"M Raymaekers","year":"2009","unstructured":"Raymaekers M, Smets R, Maes B, Cartuyvels R (2009) Checklist for optimization and validation of real-time PCR assays. J Clin Lab Anal 23(3):145\u2013151. \nhttps:\/\/doi.org\/10.1002\/jcla.20307","journal-title":"J Clin Lab Anal"},{"key":"4_CR8","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s00769-018-1319-7","volume":"23","author":"J Hedman","year":"2018","unstructured":"Hedman J, Lavander M, Salomonsson EN, Jinnerot T, Boiso L, Magnusson B, R\u00e5dstr\u00f6m P (2018) Validation guidelines for PCR workflows in bioterrorism preparedness, food safety and forensics. Accred Qual Assur 23:133\u2013144. \nhttps:\/\/doi.org\/10.1007\/s00769-018-1319-7","journal-title":"Accred Qual Assur"},{"key":"4_CR9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-60327-353-4_1","volume":"943","author":"G Johnson","year":"2013","unstructured":"Johnson G, Nolan T, Bustin SA (2013) Real-time quantitative PCR, pathogen detection and MIQE. Methods Mol Biol 943:1\u201316. \nhttps:\/\/doi.org\/10.1007\/978-1-60327-353-4_1","journal-title":"Methods Mol Biol"},{"key":"4_CR10","doi-asserted-by":"publisher","first-page":"108","DOI":"10.3389\/fmicb.2017.00108","volume":"8","author":"P Kralik","year":"2017","unstructured":"Kralik P, Ricchi M (2017) A basic guide to real time PCR in microbial diagnostics: definitions, parameters, and everything. Front Microbiol 8:108. \nhttps:\/\/doi.org\/10.3389\/fmicb.2017.00108","journal-title":"Front Microbiol"},{"key":"4_CR11","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.bdq.2017.10.001","volume":"14","author":"A Ruiz-Villalba","year":"2017","unstructured":"Ruiz-Villalba A, van Pelt-Verkuil E, Gunst QD, Ruijter JM, van den Hoff MJ (2017) Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR). Biomol Detect Quantif 14:7\u201318. \nhttps:\/\/doi.org\/10.1016\/j.bdq.2017.10.001","journal-title":"Biomol Detect Quantif"},{"key":"4_CR12","doi-asserted-by":"publisher","first-page":"495","DOI":"10.2144\/000112705","volume":"44","author":"YH Kim","year":"2008","unstructured":"Kim YH, Yang I, Bae Y-S, Park S-R (2008) Performance evaluation of thermal cyclers for PCR in a rapid cycling condition. BioTechniques 44:495\u2013496, 498, 500 passim","journal-title":"BioTechniques"},{"key":"4_CR13","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.4315\/0362-028X.JFP-10-458","volume":"74","author":"P Rossmanith","year":"2011","unstructured":"Rossmanith P, Wagner M (2011) A novel poisson distribution-based approach for testing boundaries of real-time PCR assays for food pathogen quantification. J Food Prot 74:1404\u20131412","journal-title":"J Food Prot"},{"key":"4_CR14","doi-asserted-by":"publisher","first-page":"7583","DOI":"10.1007\/s00216-016-9861-9","volume":"408","author":"AK Witte","year":"2016","unstructured":"Witte AK, Fister S, Mester P, Schoder D, Rossmanith P (2016) Evaluation of the performance of quantitative detection of the Listeria monocytogenes prfA locus with the droplet digital PCR. Anal Bioanal Chem 408:7583\u20137593","journal-title":"Anal Bioanal Chem"},{"key":"4_CR15","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1111\/j.1365-2672.2010.04915.x","volume":"110","author":"P Rossmanith","year":"2011","unstructured":"Rossmanith P, Wagner M (2011) The challenge to quantify Listeria monocytogenes- a model leading to new aspects in molecular biological food pathogen detection. J Appl Microbiol 110:605\u2013617","journal-title":"J Appl Microbiol"},{"key":"4_CR16","doi-asserted-by":"publisher","first-page":"744","DOI":"10.2144\/98245bm09","volume":"24","author":"C Lockey","year":"1998","unstructured":"Lockey C, Otto E, Long Z (1998) Real-time fluorescence detection of a single DNA molecule. BioTechniques 24:744\u2013746","journal-title":"BioTechniques"},{"key":"4_CR17","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1007\/978-1-4612-4164-5_3","volume-title":"Gene Quantif","author":"Z Wang","year":"1998","unstructured":"Wang Z, Spadoro J (1998) Determination of target copy number of quantitative standards used in pcr-based diagnostic assays. In: Gene Quantif. Birkh\u00e4user, Boston, pp 31\u201343"},{"issue":"3","key":"4_CR18","doi-asserted-by":"publisher","first-page":"e9731","DOI":"10.1371\/journal.pone.0009731","volume":"5","author":"RG Rutledge","year":"2010","unstructured":"Rutledge RG, Stewart D (2010) Assessing the performance capabilities of LRE-based assays for absolute quantitative real-time PCR. PLoS One 5(3):e9731. \nhttps:\/\/doi.org\/10.1371\/journal.pone.0009731","journal-title":"PLoS One"},{"key":"4_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.bdq.2017.04.001","volume":"12","author":"A Forootan","year":"2017","unstructured":"Forootan A, Sj\u00f6back R, Bj\u00f6rkman J, Sj\u00f6green B, Linz L, Kubista M (2017) Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR). Biomol Detect Quantif 12:1\u20136. \nhttps:\/\/doi.org\/10.1016\/j.bdq.2017.04.001","journal-title":"Biomol Detect Quantif"},{"key":"4_CR20","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1186\/1472-6750-8-47","volume":"8","author":"RG Rutledge","year":"2008","unstructured":"Rutledge RG, Stewart D (2008) A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR. BMC Biotechnol 8:47. \nhttps:\/\/doi.org\/10.1186\/1472-6750-8-47","journal-title":"BMC Biotechnol"},{"key":"4_CR21","doi-asserted-by":"publisher","first-page":"246","DOI":"10.2144\/04372RR01","volume":"37","author":"HK Nogva","year":"2004","unstructured":"Nogva HK, Rudi K (2004) Potential influence of the first PCR cycles in real-time comparative gene quantifications. BioTechniques 37:246\u2013253","journal-title":"BioTechniques"},{"issue":"13\u201314","key":"4_CR22","doi-asserted-by":"publisher","first-page":"1689","DOI":"10.1007\/s00604-013-1155-8","volume":"181","author":"JM Ruijter","year":"2014","unstructured":"Ruijter JM, Lorenz P, Tuomi JM, Hecker M, van den Hoff MJB (2014) Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency. Microchim Acta 181(13\u201314):1689\u20131696. \nhttps:\/\/doi.org\/10.1007\/s00604-013-1155-8","journal-title":"Microchim Acta"},{"issue":"1","key":"4_CR23","doi-asserted-by":"publisher","first-page":"8275","DOI":"10.1038\/s41598-018-26116-x","volume":"8","author":"AK Witte","year":"2018","unstructured":"Witte AK, Mester P, Fister S, S\u00fc\u00df B, Wagner M, Rossmanith P (2018) PCR-stop analysis as a new tool for qPCR assay validation. Sci Rep 8(1):8275. \nhttps:\/\/doi.org\/10.1038\/s41598-018-26116-x","journal-title":"Sci Rep"},{"key":"4_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00217-007-0683-z","volume":"12","author":"M Burns","year":"2008","unstructured":"Burns M, Valdivia H (2008) Modelling the limit of detection in real-time quantitative PCR. Eur Food Res Technol 12:1\u20136. \nhttps:\/\/doi.org\/10.1007\/s00217-007-0683-z","journal-title":"Eur Food Res Technol"},{"key":"4_CR25","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1007\/s12161-012-9534-z","volume":"4","author":"E Mann","year":"2012","unstructured":"Mann E, Hein I, Mester P, Stessl B, Rossmanith P, Wagner M, Dzieciol M (2012) A robust and Poisson validated quantitative 5\u2032 nuclease TaqMan\u00ae real-time PCR assay targeting fimA for the rapid detection of salmonella spp. in food. Food Anal Methods 4:991\u2013995. \nhttps:\/\/doi.org\/10.1007\/s12161-012-9534-z","journal-title":"Food Anal Methods"}],"container-title":["Methods in Molecular Biology","Listeria Monocytogenes"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-0982-8_4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T10:03:15Z","timestamp":1601028195000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-0982-8_4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,26]]},"ISBN":["9781071609811","9781071609828"],"references-count":25,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-0982-8_4","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2020,9,26]]},"assertion":[{"value":"26 September 2020","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}