{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T18:59:54Z","timestamp":1785265194059,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":31,"publisher":"Springer US","isbn-type":[{"value":"9781071611104","type":"print"},{"value":"9781071611111","type":"electronic"}],"license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021]]},"DOI":"10.1007\/978-1-0716-1111-1_5","type":"book-chapter","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T19:07:21Z","timestamp":1612897641000},"page":"83-101","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation"],"prefix":"10.1007","author":[{"given":"Megan","family":"Peppenelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Buehler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Goodrum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,9]]},"reference":[{"issue":"Pt 4","key":"5_CR1","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1099\/0022-1317-76-4-741","volume":"76","author":"C Sinzger","year":"1995","unstructured":"Sinzger C, Grefte A, Plachter B, Gouw AS, The TH, Jahn G (1995) Fibroblasts, epithelial cells, endothelial cells and smooth muscle cells are major targets of human cytomegalovirus infection in lung and gastrointestinal tissues. J Gen Virol 76(Pt 4):741\u2013750","journal-title":"J Gen Virol"},{"key":"5_CR2","doi-asserted-by":"publisher","first-page":"3166","DOI":"10.1128\/JVI.67.6.3166-3175.1993","volume":"67","author":"C Soderberg","year":"1993","unstructured":"Soderberg C, Larsson S, Bergstedt-Lindqvist S, Moller E (1993) Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection. J Virol 67:3166\u20133175","journal-title":"J Virol"},{"key":"5_CR3","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1126\/science.2997930","volume":"230","author":"RD Schrier","year":"1985","unstructured":"Schrier RD, Nelson JA, Oldstone MB (1985) Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science 230:1048\u20131051","journal-title":"Science"},{"key":"5_CR4","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1086\/517022","volume":"26","author":"M Boeckh","year":"1998","unstructured":"Boeckh M, Hoy C, Torok-Storb B (1998) Occult cytomegalovirus infection of marrow stroma. Clin Infect Dis 26:209\u2013210","journal-title":"Clin Infect Dis"},{"key":"5_CR5","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1182\/blood-2003-12-4344","volume":"104","author":"F Goodrum","year":"2004","unstructured":"Goodrum F, Jordan CT, Terhune SS, High KP, Shenk T (2004) Differential outcomes of human cytomegalovirus infection in primitive hematopoietic subpopulations. Blood 104:687\u2013695","journal-title":"Blood"},{"key":"5_CR6","doi-asserted-by":"publisher","first-page":"4140","DOI":"10.1073\/pnas.0408994102","volume":"102","author":"MB Reeves","year":"2005","unstructured":"Reeves MB, MacAry PA, Lehner PJ, Sissons JG, Sinclair JH (2005) Latency, chromatin remodeling, and reactivation of human cytomegalovirus in the dendritic cells of healthy carriers. Proc Natl Acad Sci U S A 102:4140\u20134145","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR7","doi-asserted-by":"publisher","first-page":"4526","DOI":"10.1182\/blood.V88.12.4526.bloodjournal88124526","volume":"88","author":"H Sindre","year":"1996","unstructured":"Sindre H, Tjoonnfjord GE, Rollag H, Ranneberg-Nilsen T, Veiby OP, Beck S, Degre M, Hestdal K (1996) Human cytomegalovirus suppression of and latency in early hematopoietic progenitor cells. Blood 88:4526\u20134533","journal-title":"Blood"},{"key":"5_CR8","doi-asserted-by":"publisher","first-page":"4086","DOI":"10.1182\/blood.V86.11.4086.bloodjournal86114086","volume":"86","author":"D von Laer","year":"1995","unstructured":"von Laer D, Meyer-Koenig U, Serr A, Finke J, Kanz L, Fauser AA, Neumann-Haefelin D, Brugger W, Hufert FT (1995) Detection of cytomegalovirus DNA in CD34+ cells from blood and bone marrow. Blood 86:4086\u20134090","journal-title":"Blood"},{"key":"5_CR9","doi-asserted-by":"publisher","first-page":"3937","DOI":"10.1073\/pnas.95.7.3937","volume":"95","author":"G Hahn","year":"1998","unstructured":"Hahn G, Jores R, Mocarski ES (1998) Cytomegalovirus remains latent in a common precursor of dendritic and myeloid cells. Proc Natl Acad Sci U S A 95:3937\u20133942","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR10","doi-asserted-by":"publisher","first-page":"11879","DOI":"10.1073\/pnas.91.25.11879","volume":"91","author":"K Kondo","year":"1994","unstructured":"Kondo K, Kaneshima H, Mocarski ES (1994) Human cytomegalovirus latent infection of granulocyte-macrophage progenitors. Proc Natl Acad Sci U S A 91:11879\u201311883","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR11","doi-asserted-by":"publisher","first-page":"11137","DOI":"10.1073\/pnas.93.20.11137","volume":"93","author":"K Kondo","year":"1996","unstructured":"Kondo K, Xu J, Mocarski ES (1996) Human cytomegalovirus latent gene expression in granulocyte-macrophage progenitors in culture and in seropositive individuals. Proc Natl Acad Sci U S A 93:11137\u201311142","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR12","doi-asserted-by":"publisher","first-page":"20039","DOI":"10.1073\/pnas.1014509107","volume":"107","author":"D Hargett","year":"2010","unstructured":"Hargett D, Shenk TE (2010) Experimental human cytomegalovirus latency in CD14+ monocytes. Proc Natl Acad Sci U S A 107:20039\u201320044","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR13","doi-asserted-by":"publisher","first-page":"4444","DOI":"10.1128\/JVI.78.9.4444-4453.2004","volume":"78","author":"MS Smith","year":"2004","unstructured":"Smith MS, Bentz GL, Alexander JS, Yurochko AD (2004) Human cytomegalovirus induces monocyte differentiation and migration as a strategy for dissemination and persistence. J Virol 78:4444\u20134453","journal-title":"J Virol"},{"key":"5_CR14","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/S0092-8674(01)80014-3","volume":"91","author":"C Soderberg-Naucler","year":"1997","unstructured":"Soderberg-Naucler C, Fish KN, Nelson JA (1997) Reactivation of latent human cytomegalovirus by allogeneic stimulation of blood cells from healthy donors. Cell 91:119\u2013126","journal-title":"Cell"},{"key":"5_CR15","doi-asserted-by":"publisher","first-page":"7543","DOI":"10.1128\/JVI.75.16.7543-7554.2001","volume":"75","author":"C Soderberg-Naucler","year":"2001","unstructured":"Soderberg-Naucler C, Streblow DN, Fish KN, Allan-Yorke J, Smith PP, Nelson JA (2001) Reactivation of latent human cytomegalovirus in CD14(+) monocytes is differentiation dependent. J Virol 75:7543\u20137554","journal-title":"J Virol"},{"key":"5_CR16","first-page":"123","volume-title":"Hematopoietic Stem Cell Protocols","author":"CL Miller","year":"2002","unstructured":"Miller CL, Eaves CJ (2002) Long-term culture-initiating cell assays for human and murine cells. In: Klug CA, Jordan CT (eds) Hematopoietic Stem Cell Protocols. Humana Press, Totowa, pp 123\u2013141"},{"key":"5_CR17","doi-asserted-by":"publisher","first-page":"16255","DOI":"10.1073\/pnas.252630899","volume":"99","author":"FD Goodrum","year":"2002","unstructured":"Goodrum FD, Jordan CT, High K, Shenk T (2002) Human cytomegalovirus gene expression during infection of primary hematopoietic progenitor cells: a model for latency. Proc Natl Acad Sci U S A 99:16255\u201316260","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR18","doi-asserted-by":"publisher","first-page":"E10586","DOI":"10.1073\/pnas.1710522114","volume":"114","author":"S Cheng","year":"2017","unstructured":"Cheng S, Caviness K, Buehler J, Smithey M, Nikolich-Zugich J, Goodrum F (2017) Transcriptome-wide characterization of human cytomegalovirus in natural infection and experimental latency. Proc Natl Acad Sci U S A 114:E10586\u2013E10595","journal-title":"Proc Natl Acad Sci U S A"},{"key":"5_CR19","doi-asserted-by":"publisher","first-page":"e00013","DOI":"10.1128\/mBio.00013-18","volume":"9","author":"M Shnayder","year":"2018","unstructured":"Shnayder M, Nachshon A, Krishna B, Poole E, Boshkov A, Binyamin A, Maza I, Sinclair J, Schwartz M, Stern-Ginossar N (2018) Defining the transcriptional landscape during cytomegalovirus latency with single-cell RNA sequencing. MBio 9:e00013\u2013e00018","journal-title":"MBio"},{"key":"5_CR20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005655","volume":"12","author":"J Buehler","year":"2016","unstructured":"Buehler J, Zeltzer S, Reitsma J, Petrucelli A, Umashankar M, Rak M, Zagallo P, Schroeder J, Terhune S, Goodrum F (2016) Opposing regulation of the EGF receptor: a molecular switch controlling cytomegalovirus latency and replication. PLoS Pathog 12:e1005655","journal-title":"PLoS Pathog"},{"key":"5_CR21","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1182\/blood-2007-01-070078","volume":"110","author":"F Goodrum","year":"2007","unstructured":"Goodrum F, Reeves M, Sinclair J, High K, Shenk T (2007) Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro. Blood 110:937\u2013945","journal-title":"Blood"},{"key":"5_CR22","doi-asserted-by":"publisher","first-page":"5615","DOI":"10.1128\/JVI.01989-08","volume":"83","author":"A Petrucelli","year":"2009","unstructured":"Petrucelli A, Rak M, Grainger L, Goodrum F (2009) Characterization of a novel Golgi apparatus-localized latency determinant encoded by human cytomegalovirus. J Virol 83:5615\u20135629","journal-title":"J Virol"},{"key":"5_CR23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002444","volume":"7","author":"M Umashankar","year":"2011","unstructured":"Umashankar M, Petrucelli A, Cicchini L, Caposio P, Kreklywich CN, Rak M, Bughio F, Goldman DC, Hamlin KL, Nelson JA, Fleming WH, Streblow DN, Goodrum F (2011) A novel human cytomegalovirus locus modulates cell type-specific outcomes of infection. PLoS Pathog 7:e1002444","journal-title":"PLoS Pathog"},{"key":"5_CR24","doi-asserted-by":"publisher","first-page":"14412","DOI":"10.1128\/JVI.02711-14","volume":"88","author":"K Caviness","year":"2014","unstructured":"Caviness K, Cicchini L, Rak M, Umashankar M, Goodrum F (2014) Complex expression of the UL136 gene of human cytomegalovirus results in multiple protein isoforms with unique roles in replication. J Virol 88:14412\u201314425","journal-title":"J Virol"},{"key":"5_CR25","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01986-15","volume":"7","author":"K Caviness","year":"2016","unstructured":"Caviness K, Bughio F, Crawford LB, Streblow DN, Nelson JA, Caposio P, Goodrum F (2016) Complex interplay of the UL136 isoforms balances cytomegalovirus replication and latency. MBio 7:e01986","journal-title":"MBio"},{"key":"5_CR26","doi-asserted-by":"publisher","first-page":"2959","DOI":"10.1128\/JVI.02507-15","volume":"90","author":"MS Humby","year":"2015","unstructured":"Humby MS, O\u2019Connor CM (2015) Human cytomegalovirus US28 is important for latent infection of hematopoietic progenitor cells. J Virol 90:2959\u20132970","journal-title":"J Virol"},{"key":"5_CR27","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02035-14","volume":"5","author":"JD MacManiman","year":"2014","unstructured":"MacManiman JD, Meuser A, Botto S, Smith PP, Liu F, Jarvis MA, Nelson JA, Caposio P (2014) Human cytomegalovirus-encoded pUL7 is a novel CEACAM1-like molecule responsible for promotion of angiogenesis. MBio 5:e02035","journal-title":"MBio"},{"key":"5_CR28","first-page":"104","volume":"1","author":"MH Hancock","year":"2017","unstructured":"Hancock MH, Nelson JA (2017) Modulation of the NFkappab Signalling pathway by human cytomegalovirus. Virology (Hyderabad) 1:104","journal-title":"Virology (Hyderabad)"},{"key":"5_CR29","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1016\/j.chom.2010.08.001","volume":"8","author":"MS Smith","year":"2010","unstructured":"Smith MS, Goldman DC, Bailey AS, Pfaffle DL, Kreklywich CN, Spencer DB, Othieno FA, Streblow DN, Garcia JV, Fleming WH, Nelson JA (2010) Granulocyte-colony stimulating factor reactivates human cytomegalovirus in a latently infected humanized mouse model. Cell Host Microbe 8:284\u2013291","journal-title":"Cell Host Microbe"},{"key":"5_CR30","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.jim.2009.06.008","volume":"347","author":"Y Hu","year":"2009","unstructured":"Hu Y, Smyth GK (2009) ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays. J Immunol Methods 347:70\u201378","journal-title":"J Immunol Methods"},{"key":"5_CR31","doi-asserted-by":"publisher","unstructured":"Crawford LB, Caposio P, Kreklywich C, Pham AH, Hancock MH, Jones TA, Smith PP, Yurochko AD, Nelson JA, Streblow DN (2019) Cite share human cytomegalovirus US28 ligand binding activity is required for latency in CD34(+) hematopoietic progenitor cells and humanized NSG mice. mBio 20;10(4):e01889\u201319. https:\/\/doi.org\/10.1128\/mBio.01889-19. PMID: 31431555","DOI":"10.1128\/mBio.01889-19"}],"container-title":["Methods in Molecular Biology","Human Cytomegaloviruses"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-1111-1_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T19:07:50Z","timestamp":1612897670000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/978-1-0716-1111-1_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"ISBN":["9781071611104","9781071611111"],"references-count":31,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-1111-1_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]},"assertion":[{"value":"9 February 2021","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}