{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:56:19Z","timestamp":1791402979892,"version":"4.3.3"},"publisher-location":"New York, NY","reference-count":8,"publisher":"Springer US","isbn-type":[{"value":"9781071619896","type":"print"},{"value":"9781071619902","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T00:00:00Z","timestamp":1649808000000},"content-version":"vor","delay-in-days":102,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Axonal transport is essential for the development, function, and survival of the nervous system. In an energy-demanding process, motor proteins act in concert with microtubules to deliver cargoes, such as organelles, from one end of the axon to the other. Perturbations in axonal transport are a prominent phenotype of many neurodegenerative diseases, including amyotrophic lateral sclerosis. Here, we describe a simple method to\u00a0fluorescently label mitochondrial cargo, a surrogate for fast axonal transport, in human induced pluripotent stem cell\u2013derived motor neurons. This method enables the sparse labeling of axons to track directionality of movement and can be adapted to assess not only the cell autonomous effects of a genetic mutation on axonal transport but also the cell non-autonomous effects, through the use of conditioned medium and\/or co-culture systems.<\/jats:p>","DOI":"10.1007\/978-1-0716-1990-2_16","type":"book-chapter","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:04:20Z","timestamp":1649772260000},"page":"311-322","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of Mitochondrial Trafficking as a Surrogate for Fast Axonal Transport in Human Induced Pluripotent Stem Cell\u2013Derived Spinal Motor Neurons"],"prefix":"10.1007","author":[{"given":"Arpan R.","family":"Mehta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharthan","family":"Chandran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhuvaneish T.","family":"Selvaraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,13]]},"reference":[{"issue":"12","key":"16_CR1","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1038\/s41582-019-0257-2","volume":"15","author":"JN Sleigh","year":"2019","unstructured":"Sleigh JN, Rossor AM, Fellows AD, Tosolini AP, Schiavo G (2019) Axonal transport and neurological disease. Nat Rev Neurol 15(12):691\u2013703. https:\/\/doi.org\/10.1038\/s41582-019-0257-2","journal-title":"Nat Rev Neurol"},{"issue":"2","key":"16_CR2","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1007\/s00401-020-02252-5","volume":"141","author":"AR Mehta","year":"2021","unstructured":"Mehta AR, Gregory JM, Dando O, Carter RN, Burr K, Nanda J, Story D, McDade K, Smith C, Morton NM, Mahad DJ, Hardingham GE, Chandran S, Selvaraj BT (2021) Mitochondrial bioenergetic deficits in C9orf72 amyotrophic lateral sclerosis motor neurons cause dysfunctional axonal homeostasis. Acta Neuropathol 141(2):257\u2013279. https:\/\/doi.org\/10.1007\/s00401-020-02252-5","journal-title":"Acta Neuropathol"},{"issue":"1","key":"16_CR3","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1038\/nbt.3049","volume":"33","author":"Y Maury","year":"2015","unstructured":"Maury Y, Come J, Piskorowski RA, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S (2015) Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol 33(1):89\u201396. https:\/\/doi.org\/10.1038\/nbt.3049","journal-title":"Nat Biotechnol"},{"issue":"1","key":"16_CR4","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1038\/s41467-017-02729-0","volume":"9","author":"BT Selvaraj","year":"2018","unstructured":"Selvaraj BT, Livesey MR, Zhao C, Gregory JM, James OT, Cleary EM, Chouhan AK, Gane AB, Perkins EM, Dando O, Lillico SG, Lee YB, Nishimura AL, Poreci U, Thankamony S, Pray M, Vasistha NA, Magnani D, Borooah S, Burr K, Story D, McCampbell A, Shaw CE, Kind PC, Aitman TJ, Whitelaw CBA, Wilmut I, Smith C, Miles GB, Hardingham GE, Wyllie DJA, Chandran S (2018) C9ORF72 repeat expansion causes vulnerability of motor neurons to Ca(2+)-permeable AMPA receptor-mediated excitotoxicity. Nat Commun 9(1):347. https:\/\/doi.org\/10.1038\/s41467-017-02729-0","journal-title":"Nat Commun"},{"key":"16_CR5","doi-asserted-by":"publisher","unstructured":"Fumagalli L, Young FL, Boeynaems S, De Decker M, Mehta AR, Swijsen A, Fazal R, Guo W, Moisse M, Beckers J, Dedeene L, Selvaraj BT, Vandoorne T, Madan V, van Blitterswijk M, Raitcheva D, McCampbell A, Poesen K, Gitler AD, Koch P, Berghe PV, Thal DR, Verfaillie C, Chandran S, Van Den Bosch L, Bullock SL, Van Damme P (2021) C9orf72-derived arginine-containing dipeptide repeats associate with axonal transport machinery and impede microtubule-based motility. Sci Adv. 7(15):eabg3013. https:\/\/doi.org\/10.1126\/sciadv.abg3013. PMID: 33837088; PMCID: PMC8034861.","DOI":"10.1126\/sciadv.abg3013"},{"issue":"7","key":"16_CR6","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez JY, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9(7):676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019","journal-title":"Nat Methods"},{"issue":"3","key":"16_CR7","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/j.cell.2012.12.029","volume":"152","author":"D Zala","year":"2013","unstructured":"Zala D, Hinckelmann MV, Yu H, Lyra da Cunha MM, Liot G, Cordelieres FP, Marco S, Saudou F (2013) Vesicular glycolysis provides on-board energy for fast axonal transport. Cell 152(3):479\u2013491. https:\/\/doi.org\/10.1016\/j.cell.2012.12.029","journal-title":"Cell"},{"key":"16_CR8","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1007\/978-1-0716-0219-5_20","volume":"2101","author":"S Bodakuntla","year":"2020","unstructured":"Bodakuntla S, Magiera MM, Janke C (2020) Measuring the impact of tubulin posttranslational modifications on axonal transport. Methods Mol Biol 2101:353\u2013370. https:\/\/doi.org\/10.1007\/978-1-0716-0219-5_20","journal-title":"Methods Mol Biol"}],"container-title":["Methods in Molecular Biology","Axonal Transport"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-1990-2_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:05:43Z","timestamp":1649772343000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-1990-2_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"ISBN":["9781071619896","9781071619902"],"references-count":8,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-1990-2_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"13 April 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}