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They are widespread in all the seas, constituting abundant communities in various ecosystems. Among chordates, only tunicates are able to reproduce asexually, forming colonies. The high regenerative potentialities enabling tunicates to regenerate damaged body parts, or the whole body, represent a peculiarity of this taxon. Here we review the methodological approaches used in more than a century of biological studies to induce regeneration in both solitary and colonial species. For solitary species, we refer to the regeneration of single organs or body parts (e.g., siphon, brain, gonad, tunic, viscera). For colonial species, we review a plethora of experiments regarding the surgical manipulation of colonies, the regeneration of isolated colonial entities, such as single buds in the tunic, or part of tunic and its circulatory system.<\/jats:p>","DOI":"10.1007\/978-1-0716-2172-1_2","type":"book-chapter","created":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T13:19:31Z","timestamp":1648732771000},"page":"27-48","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Studying Regeneration in Ascidians: An Historical Overview"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6886-3273","authenticated-orcid":false,"given":"Virginia","family":"Vanni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loriano","family":"Ballarin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Gasparini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3675-6339","authenticated-orcid":false,"given":"Anna","family":"Peronato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Manni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,1]]},"reference":[{"key":"2_CR1","first-page":"521","volume-title":"Conn\u2019s handbook of models for human aging, second Edi","author":"WR Jeffery","year":"2018","unstructured":"Jeffery WR (2018) Regeneration and aging in the tunicate Ciona intestinalis. 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