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Studying the expression pattern of <jats:italic>var<\/jats:italic> genes in <jats:italic>P. falciparum<\/jats:italic> clinical isolates is crucial for understanding disease mechanism and immunity to malaria. However, <jats:italic>var<\/jats:italic> genes are highly variable, which makes it difficult to study their expression in clinical isolates obtained directly from malaria patients. In this chapter, we describe an approach for analysis of <jats:italic>var<\/jats:italic> gene expression that targets a region referred to as DBL\u03b1 tag, which is relatively conserved in all <jats:italic>var<\/jats:italic> genes.<\/jats:p>","DOI":"10.1007\/978-1-0716-2189-9_14","type":"book-chapter","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:06:06Z","timestamp":1658840766000},"page":"173-184","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Analysis of var Gene Transcription Pattern Using DBL\u03b1 Tags"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0545-9665","authenticated-orcid":false,"given":"Kivisi C.","family":"Andisi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdirahman I.","family":"Abdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"14_CR1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature01097","volume":"419","author":"MJ Gardner","year":"2002","unstructured":"Gardner MJ, Hall N, Fung E et al (2002) Genome sequence of the human malaria parasite plasmodium falciparum. 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