{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T02:29:42Z","timestamp":1789525782510,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":19,"publisher":"Springer US","isbn-type":[{"value":"9781071621882","type":"print"},{"value":"9781071621899","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022]]},"DOI":"10.1007\/978-1-0716-2189-9_17","type":"book-chapter","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:06:06Z","timestamp":1658840766000},"page":"221-239","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["CRISPR\/Cas9 Editing of the Plasmodium falciparum Genome"],"prefix":"10.1007","author":[{"given":"Sophie","family":"Adjalley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4973-0915","authenticated-orcid":false,"given":"Marcus Chee San","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"issue":"4","key":"17_CR1","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1038\/nmeth.4223","volume":"14","author":"J Birnbaum","year":"2017","unstructured":"Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T (2017) A genetic system to study Plasmodium falciparum protein function. Nat Methods 14(4):450\u2013456. https:\/\/doi.org\/10.1038\/nmeth.4223","journal-title":"Nat Methods"},{"issue":"6405","key":"17_CR2","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1126\/science.aat5011","volume":"361","author":"GJ Knott","year":"2018","unstructured":"Knott GJ, Doudna JA (2018) CRISPR-Cas guides the future of genetic engineering. Science 361(6405):866\u2013869. https:\/\/doi.org\/10.1126\/science.aat5011","journal-title":"Science"},{"issue":"3","key":"17_CR3","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1128\/MMBR.00059-13","volume":"78","author":"AH Lee","year":"2014","unstructured":"Lee AH, Symington LS, Fidock DA (2014) DNA repair mechanisms and their biological roles in the malaria parasite Plasmodium falciparum. Microbiol Mol Biol Rev 78(3):469\u2013486. https:\/\/doi.org\/10.1128\/MMBR.00059-13","journal-title":"Microbiol Mol Biol Rev"},{"issue":"8","key":"17_CR4","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1038\/nbt.2925","volume":"32","author":"M Ghorbal","year":"2014","unstructured":"Ghorbal M, Gorman M, Macpherson CR, Martins RM, Scherf A, Lopez-Rubio JJ (2014) Genome editing in the human malaria parasite plasmodium falciparum using the CRISPR-Cas9 system. Nat Biotechnol 32(8):819\u2013821. https:\/\/doi.org\/10.1038\/nbt.2925","journal-title":"Nat Biotechnol"},{"key":"17_CR5","doi-asserted-by":"publisher","first-page":"16166","DOI":"10.1038\/nmicrobiol.2016.166","volume":"1","author":"MY Lim","year":"2016","unstructured":"Lim MY, LaMonte G, Lee MCS, Reimer C, Tan BH, Corey V, Tjahjadi BF, Chua A, Nachon M, Wintjens R, Gedeck P, Malleret B, Renia L, Bonamy GMC, Ho Fig PC, Yeung BKS, Chow ED, Lim L, Fidock DA, Diagana TT, Winzeler EA, Bifani P (2016) UDP-galactose and acetyl-CoA transporters as Plasmodium multidrug resistance genes. Nat Microbiol 1:16166. https:\/\/doi.org\/10.1038\/nmicrobiol.2016.166","journal-title":"Nat Microbiol"},{"issue":"9","key":"17_CR6","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1038\/nmeth.3063","volume":"11","author":"JC Wagner","year":"2014","unstructured":"Wagner JC, Platt RJ, Goldfless SJ, Zhang F, Niles JC (2014) Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum. Nat Methods 11(9):915\u2013918. https:\/\/doi.org\/10.1038\/nmeth.3063","journal-title":"Nat Methods"},{"issue":"5","key":"17_CR7","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1093\/bfgp\/elz012","volume":"18","author":"MCS Lee","year":"2019","unstructured":"Lee MCS, Lindner SE, Lopez-Rubio JJ, Llinas M (2019) Cutting back malaria: CRISPR\/Cas9 genome editing of Plasmodium. Brief Funct Genomics 18(5):281\u2013289. https:\/\/doi.org\/10.1093\/bfgp\/elz012","journal-title":"Brief Funct Genomics"},{"issue":"1","key":"17_CR8","doi-asserted-by":"publisher","first-page":"e01385","DOI":"10.1128\/AAC.01385-20","volume":"65","author":"TP Remcho","year":"2020","unstructured":"Remcho TP, Guggilapu SD, Cruz P, Nardone GA, Heffernan G, O'Connor RD, Bewley CA, Wellems TE, Lane KD (2020) Regioisomerization of antimalarial drug WR99210 explains the inactivity of a commercial stock. Antimicrob Agents Chemother 65(1):e01385. https:\/\/doi.org\/10.1128\/AAC.01385-20","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"17_CR9","doi-asserted-by":"publisher","first-page":"1479","DOI":"10.1016\/j.cell.2013.12.001","volume":"155","author":"B Chen","year":"2013","unstructured":"Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang B (2013) Dynamic imaging of genomic loci in living human cells by an optimized CRISPR\/Cas system. Cell 155(7):1479\u20131491. https:\/\/doi.org\/10.1016\/j.cell.2013.12.001","journal-title":"Cell"},{"issue":"8","key":"17_CR10","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1038\/nbt.3291","volume":"33","author":"CR MacPherson","year":"2015","unstructured":"MacPherson CR, Scherf A (2015) Flexible guide-RNA design for CRISPR applications using Protospacer workbench. Nat Biotechnol 33(8):805\u2013806. https:\/\/doi.org\/10.1038\/nbt.3291","journal-title":"Nat Biotechnol"},{"issue":"Web Server issu","key":"17_CR11","doi-asserted-by":"publisher","first-page":"W401","DOI":"10.1093\/nar\/gku410","volume":"42","author":"TG Montague","year":"2014","unstructured":"Montague TG, Cruz JM, Gagnon JA, Church GM, Valen E (2014) CHOPCHOP: a CRISPR\/Cas9 and TALEN web tool for genome editing. Nucleic Acids Res 42(Web Server issue):W401\u2013W407. https:\/\/doi.org\/10.1093\/nar\/gku410","journal-title":"Nucleic Acids Res"},{"issue":"4","key":"17_CR12","doi-asserted-by":"publisher","first-page":"e000033","DOI":"10.1099\/mgen.0.000033","volume":"1","author":"D Peng","year":"2015","unstructured":"Peng D, Tarleton R (2015) EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens. Microb Genom 1(4):e000033. https:\/\/doi.org\/10.1099\/mgen.0.000033","journal-title":"Microb Genom"},{"issue":"11","key":"17_CR13","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/j.ijpara.2018.03.009","volume":"48","author":"JM Ribeiro","year":"2018","unstructured":"Ribeiro JM, Garriga M, Potchen N, Crater AK, Gupta A, Ito D, Desai SA (2018) Guide RNA selection for CRISPR-Cas9 transfections in Plasmodium falciparum. Int J Parasitol 48(11):825\u2013832. https:\/\/doi.org\/10.1016\/j.ijpara.2018.03.009","journal-title":"Int J Parasitol"},{"issue":"1","key":"17_CR14","doi-asserted-by":"publisher","first-page":"10894","DOI":"10.1038\/s41598-020-67791-z","volume":"10","author":"M Carrasquilla","year":"2020","unstructured":"Carrasquilla M, Adjalley S, Sanderson T, Marin-Menendez A, Coyle R, Montandon R, Rayner JC, Pance A, Lee MCS (2020) Defining multiplicity of vector uptake in transfected Plasmodium parasites. Sci Rep 10(1):10894. https:\/\/doi.org\/10.1038\/s41598-020-67791-z","journal-title":"Sci Rep"},{"issue":"24","key":"17_CR15","doi-asserted-by":"publisher","first-page":"9555","DOI":"10.1074\/jbc.RA118.007121","volume":"294","author":"MP Walker","year":"2019","unstructured":"Walker MP, Lindner SE (2019) Ribozyme-mediated, multiplex CRISPR gene editing and CRISPR interference (CRISPRi) in rodent-infectious Plasmodium yoelii. J Biol Chem 294(24):9555\u20139566. https:\/\/doi.org\/10.1074\/jbc.RA118.007121","journal-title":"J Biol Chem"},{"issue":"4","key":"17_CR16","doi-asserted-by":"publisher","first-page":"e00729","DOI":"10.1128\/mBio.00729-17","volume":"8","author":"JM Bryant","year":"2017","unstructured":"Bryant JM, Regnault C, Scheidig-Benatar C, Baumgarten S, Guizetti J, Scherf A (2017) CRISPR\/Cas9 genome editing reveals that the intron is not essential for var2csa gene activation or silencing in Plasmodium falciparum. mBio 8(4):e00729. https:\/\/doi.org\/10.1128\/mBio.00729-17","journal-title":"mBio"},{"issue":"4","key":"17_CR17","doi-asserted-by":"publisher","first-page":"e00547","DOI":"10.1128\/mBio.00547-17","volume":"8","author":"SF Calhoun","year":"2017","unstructured":"Calhoun SF, Reed J, Alexander N, Mason CE, Deitsch KW, Kirkman LA (2017) Chromosome end repair and genome stability in Plasmodium falciparum. mBio 8(4):e00547. https:\/\/doi.org\/10.1128\/mBio.00547-17","journal-title":"mBio"},{"key":"17_CR18","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1186\/s13059-015-0846-3","volume":"16","author":"Y Dang","year":"2015","unstructured":"Dang Y, Jia G, Choi J, Ma H, Anaya E, Ye C, Shankar P, Wu H (2015) Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency. Genome Biol 16:280. https:\/\/doi.org\/10.1186\/s13059-015-0846-3","journal-title":"Genome Biol"},{"issue":"5","key":"17_CR19","doi-asserted-by":"publisher","first-page":"e0178163","DOI":"10.1371\/journal.pone.0178163","volume":"12","author":"ED Crawford","year":"2017","unstructured":"Crawford ED, Quan J, Horst JA, Ebert D, Wu W, DeRisi JL (2017) Plasmid-free CRISPR\/Cas9 genome editing in Plasmodium falciparum confirms mutations conferring resistance to the dihydroisoquinolone clinical candidate SJ733. PLoS One 12(5):e0178163. https:\/\/doi.org\/10.1371\/journal.pone.0178163","journal-title":"PLoS One"}],"container-title":["Methods in Molecular Biology","Malaria Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2189-9_17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:08:15Z","timestamp":1658840895000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2189-9_17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"ISBN":["9781071621882","9781071621899"],"references-count":19,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2189-9_17","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"27 July 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}