{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:12:58Z","timestamp":1790647978352,"version":"4.1.0"},"publisher-location":"New York, NY","reference-count":24,"publisher":"Springer US","isbn-type":[{"value":"9781071621882","type":"print"},{"value":"9781071621899","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022]]},"DOI":"10.1007\/978-1-0716-2189-9_5","type":"book-chapter","created":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:06:06Z","timestamp":1658840766000},"page":"37-49","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Establishing and Maintaining In Vitro Cultures of Asexual Blood Stages of Plasmodium falciparum"],"prefix":"10.1007","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9876-0248","authenticated-orcid":false,"given":"Mary","family":"Lopez-Perez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8017-2491","authenticated-orcid":false,"given":"Zakaria","family":"Seidu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,7,27]]},"reference":[{"key":"5_CR1","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1126\/science.781840","volume":"193","author":"W Trager","year":"1976","unstructured":"Trager W, Jensen JB (1976) Human malaria parasites in continuous culture. Science 193:673\u2013675","journal-title":"Science"},{"key":"5_CR2","doi-asserted-by":"publisher","first-page":"883","DOI":"10.2307\/3279900","volume":"63","author":"JB Jensen","year":"1977","unstructured":"Jensen JB, Trager W (1977) Plasmodium falciparum in culture: use of outdated erthrocytes and description of the candle jar method. J Parasitol 63:883\u2013886","journal-title":"J Parasitol"},{"key":"5_CR3","doi-asserted-by":"publisher","first-page":"35","DOI":"10.2147\/HP.S92198","volume":"3","author":"RH Wenger","year":"2015","unstructured":"Wenger RH, Kurtcuoglu V, Scholz CC, Marti HH, Hoogewijs D (2015) Frequently asked questions in hypoxia research. Hypoxia 3:35\u201343","journal-title":"Hypoxia"},{"key":"5_CR4","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1016\/j.pt.2018.04.005","volume":"34","author":"S Duffy","year":"2018","unstructured":"Duffy S, Avery VM (2018) Routine in vitro culture of Plasmodium falciparum: experimental consequences? Trends Parasitol 34:564\u2013575","journal-title":"Trends Parasitol"},{"key":"5_CR5","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1475-2875-10-4","volume":"10","author":"M Torrentino-Madamet","year":"2011","unstructured":"Torrentino-Madamet M, Almeras L, Desplans J, Le Priol Y, Belghazi M, Pophillat M, Fourquet P, Jammes Y, Parzy D (2011) Global response of Plasmodium falciparum to hyperoxia: a combined transcriptomic and proteomic approach. Malar J 10:4","journal-title":"Malar J"},{"key":"5_CR6","doi-asserted-by":"publisher","first-page":"418","DOI":"10.2307\/3280287","volume":"65","author":"C Lambros","year":"1979","unstructured":"Lambros C, Vanderberg JP (1979) Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol 65:418\u2013420","journal-title":"J Parasitol"},{"key":"5_CR7","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/S0035-9203(97)90110-3","volume":"91","author":"SL Cranmer","year":"1997","unstructured":"Cranmer SL, Magowan C, Liang J, Coppel RL, Cooke BM (1997) An alternative to serum for cultivation of Plasmodium falciparum in vitro. Trans R Soc Trop Med Hyg 91:363\u2013365","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"5_CR8","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1038\/nprot.2009.198","volume":"4","author":"A Radfar","year":"2009","unstructured":"Radfar A, Mendez D, Moneriz C, Linares M, Marin-Garcia P, Puyet A, Diez A, Bautista JM (2009) Synchronous culture of Plasmodium falciparum at high parasitemia levels. Nat Protoc 4:1899\u20131915","journal-title":"Nat Protoc"},{"key":"5_CR9","volume-title":"Freezing of patient isolates and strains with glycerolyte","author":"HA Ismail","year":"2013","unstructured":"Ismail HA (2013) Freezing of patient isolates and strains with glycerolyte, 6th edn. MR4\/ATCC","edition":"6"},{"key":"5_CR10","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/S0011-2240(68)80154-3","volume":"5","author":"AW Rowe","year":"1968","unstructured":"Rowe AW, Eyster E, Kellner A (1968) Liquid nitrogen preservation of red blood cells for transfusion; a low glycerol-rapid freeze procedure. Cryobiology 5:119\u2013128","journal-title":"Cryobiology"},{"key":"5_CR11","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.1038\/nprot.2016.096","volume":"11","author":"MJ Delves","year":"2016","unstructured":"Delves MJ, Straschil U, Ruecker A, Miguel-Blanco C, Marques S, Dufour AC, Baum J, Sinden RE (2016) Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions. Nat Protoc 11:1668\u20131680","journal-title":"Nat Protoc"},{"key":"5_CR12","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1128\/CMR.15.3.355-364.2002","volume":"15","author":"FL Schuster","year":"2002","unstructured":"Schuster FL (2002) Cultivation of Plasmodium spp. Clin Microbiol Rev 15:355\u2013364","journal-title":"Clin Microbiol Rev"},{"key":"5_CR13","doi-asserted-by":"publisher","first-page":"743","DOI":"10.4269\/ajtmh.1978.27.743","volume":"27","author":"JB Jensen","year":"1978","unstructured":"Jensen JB, Trager W (1978) Plasmodium falciparum in culture: establishment of additional strains. Am J Trop Med Hyg 27:743\u2013746","journal-title":"Am J Trop Med Hyg"},{"key":"5_CR14","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/S0035-9203(99)90120-7","volume":"93","author":"G Snounou","year":"1999","unstructured":"Snounou G, Zhu X, Siripoon N, Jarra W, Thaithong S, Brown KN, Viriyakosol S (1999) Biased distribution of msp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans R Soc Trop Med Hyg 93:369\u2013374","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"5_CR15","first-page":"1","volume":"67","author":"IW Sherman","year":"2009","unstructured":"Sherman IW (2009) Reflections on a century of malaria biochemistry: salvage of purines. Adv Parasitol 67:1\u2013402","journal-title":"Adv Parasitol"},{"key":"5_CR16","volume-title":"Basic malaria microscopy","author":"WHO","year":"2010","unstructured":"WHO (2010) Basic malaria microscopy, 2nd edn. WHO, Geneva","edition":"2"},{"key":"5_CR17","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1152\/physrev.2001.81.2.495","volume":"81","author":"K Kirk","year":"2001","unstructured":"Kirk K (2001) Membrane transport in the malaria-infected erythrocyte. Physiol Rev 81:495\u2013537","journal-title":"Physiol Rev"},{"key":"5_CR18","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1016\/0035-9203(84)90221-9","volume":"78","author":"AR Dluzewski","year":"1984","unstructured":"Dluzewski AR, Ling IT, Rangachari K, Bates PA, Wilson RJ (1984) A simple method for isolating viable mature parasites of Plasmodium falciparum from cultures. Trans R Soc Trop Med Hyg 78:622\u2013624","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"5_CR19","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1080\/00034983.1994.11812859","volume":"88","author":"ID Goodyer","year":"1994","unstructured":"Goodyer ID, Johnson J, Eisenthal R, Hayes DJ (1994) Purification of mature-stage Plasmodium falciparum by gelatine flotation. Ann Trop Med Parasitol 88:209\u2013211","journal-title":"Ann Trop Med Parasitol"},{"key":"5_CR20","first-page":"7","volume":"4","author":"AC Uhlemann","year":"2000","unstructured":"Uhlemann AC, Staalsoe T, Klinkert MQ, Hviid L (2000) Analysis of Plasmodium falciparum-infected red blood cells. MACS 4:7\u20138","journal-title":"MACS"},{"key":"5_CR21","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/1475-2875-7-45","volume":"7","author":"C Ribaut","year":"2008","unstructured":"Ribaut C, Berry A, Chevalley S, Reybier K, Morlais I, Parzy D, Nepveu F, Benoit-Vical F, Valentin A (2008) Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species. Malar J 7:45","journal-title":"Malar J"},{"key":"5_CR22","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.2307\/3280864","volume":"69","author":"MD Jensen","year":"1983","unstructured":"Jensen MD, Conley M, Helstowski LD (1983) Culture of Plasmodium falciparum: the role of pH, glucose, and lactate. J Parasitol 69:1060\u20131067","journal-title":"J Parasitol"},{"key":"5_CR23","doi-asserted-by":"publisher","first-page":"6436","DOI":"10.1038\/s41598-017-06295-9","volume":"7","author":"L Murray","year":"2017","unstructured":"Murray L, Stewart LB, Tarr SJ, Ahouidi AD, Diakite M, Amambua-Ngwa A, Conway DJ (2017) Multiplication rate variation in the human malaria parasite Plasmodium falciparum. Sci Rep 7:6436","journal-title":"Sci Rep"},{"key":"5_CR24","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1111\/febs.14370","volume":"285","author":"ES Chou","year":"2018","unstructured":"Chou ES, Abidi SZ, Teye M, Leliwa-Sytek A, Rask TS, Cobbold SA, Tonkin-Hill GQ, Subramaniam KS, Sexton AE, Creek DJ, Daily JP, Duffy MF, Day KP (2018) A high parasite density environment induces transcriptional changes and cell death in Plasmodium falciparum blood stages. FEBS J 285:848\u2013870","journal-title":"FEBS J"}],"container-title":["Methods in Molecular Biology","Malaria Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2189-9_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,26]],"date-time":"2022-07-26T13:06:21Z","timestamp":1658840781000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2189-9_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"ISBN":["9781071621882","9781071621899"],"references-count":24,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2189-9_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"27 July 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}