{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T16:14:59Z","timestamp":1770826499035,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":19,"publisher":"Springer US","isbn-type":[{"value":"9781071623671","type":"print"},{"value":"9781071623688","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022]]},"DOI":"10.1007\/978-1-0716-2368-8_16","type":"book-chapter","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T16:21:33Z","timestamp":1656606093000},"page":"313-325","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor"],"prefix":"10.1007","author":[{"given":"Lukas","family":"Helfinger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher G.","family":"Tate","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,7,1]]},"reference":[{"key":"16_CR1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.cell.2017.11.033","volume":"172","author":"AS Hauser","year":"2018","unstructured":"Hauser AS, Chavali S, Masuho I et al (2018) Pharmacogenomics of GPCR drug targets. Cell 172:41\u201354.e19","journal-title":"Cell"},{"key":"16_CR2","doi-asserted-by":"crossref","unstructured":"Costagliola S, Dias JA, Gershengorn M, et al (2020) Glycoprotein hormone receptors (version 2020.4) in the IUPHAR\/BPS guide to pharmacology database. IUPHAR\/BPS Guid to Pharmacol CITE 2020","DOI":"10.2218\/gtopdb\/F30\/2020.4"},{"key":"16_CR3","doi-asserted-by":"publisher","first-page":"1301","DOI":"10.1007\/PL00000941","volume":"58","author":"P Kopp","year":"2001","unstructured":"Kopp P (2001) The TSH receptor and its role in thyroid disease. Cell Mol Life Sci 58:1301\u20131322","journal-title":"Cell Mol Life Sci"},{"key":"16_CR4","doi-asserted-by":"publisher","first-page":"13733","DOI":"10.1016\/S0021-9258(17)36705-4","volume":"269","author":"A Allgeier","year":"1994","unstructured":"Allgeier A, Offermanns S, Van Sande J et al (1994) The human thyrotropin receptor activates G-proteins Gs and Gq\/11. J Biol Chem 269:13733\u201313735","journal-title":"J Biol Chem"},{"key":"16_CR5","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1210\/er.2008-0044","volume":"30","author":"G Kleinau","year":"2009","unstructured":"Kleinau G, Krause G (2009) Thyrotropin and homologous glycoprotein hormone receptors: structural and functional aspects of extracellular signaling mechanisms. Endocr Rev 30:133\u2013151","journal-title":"Endocr Rev"},{"key":"16_CR6","doi-asserted-by":"publisher","first-page":"86","DOI":"10.3389\/fendo.2017.00086","volume":"8","author":"G Kleinau","year":"2017","unstructured":"Kleinau G, Worth CL, Kreuchwig A et al (2017) Structural\u2013functional features of the thyrotropin receptor: a Class A G-protein-coupled receptor at work. Front Endocrinol (Lausanne) 8:86","journal-title":"Front Endocrinol (Lausanne)"},{"key":"16_CR7","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1016\/j.mce.2013.08.021","volume":"382","author":"X Jiang","year":"2014","unstructured":"Jiang X, Dias JA, He X (2014) Structural biology of glycoprotein hormones and their receptors: insights to signaling. Mol Cell Endocrinol 382:424\u2013451","journal-title":"Mol Cell Endocrinol"},{"key":"16_CR8","doi-asserted-by":"publisher","first-page":"12491","DOI":"10.1073\/pnas.1206643109","volume":"109","author":"X Jiang","year":"2012","unstructured":"Jiang X, Liu H, Chen X et al (2012) Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor. Proc Natl Acad Sci U S A 109:12491\u201312496","journal-title":"Proc Natl Acad Sci U S A"},{"key":"16_CR9","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1677\/JME-08-0152","volume":"42","author":"R N\u00fa\u00f1ez Miguel","year":"2009","unstructured":"N\u00fa\u00f1ez Miguel R, Sanders J, Chirgadze DY et al (2009) Thyroid stimulating autoantibody M22 mimics TSH binding to the TSH receptor leucine rich domain: a comparative structural study of protein\u2013protein interactions. J Mol Endocrinol 42:381\u2013395","journal-title":"J Mol Endocrinol"},{"key":"16_CR10","doi-asserted-by":"crossref","unstructured":"Rapoport B and McLachlan SM (2016), TSH receptor cleavage into subunits and shedding of the A-subunit; a molecular and clinical perspective. https:\/\/academic.oup.com\/edrv\/article-lookup\/doi\/10.1210\/er.2015-1098","DOI":"10.1210\/er.2015-1098"},{"key":"16_CR11","first-page":"2129","volume":"82","author":"A Kakinuma","year":"1997","unstructured":"Kakinuma A, Chazenbalk GD, Jaume JC et al (1997) The human thyrotropin (TSH) receptor in a TSH binding inhibition assay for TSH receptor autoantibodies. J Clin Endocrinol Metab 82:2129\u20132134","journal-title":"J Clin Endocrinol Metab"},{"key":"16_CR12","doi-asserted-by":"publisher","first-page":"1959","DOI":"10.1074\/jbc.273.4.1959","volume":"273","author":"K Tanaka","year":"1998","unstructured":"Tanaka K, Chazenbalk GD, McLachlan SM et al (1998) Thyrotropin receptor cleavage at site 1 does not involve a specific amino acid motif but instead depends on the presence of the unique, 50 amino acid insertion. J Biol Chem 273:1959\u20131963","journal-title":"J Biol Chem"},{"key":"16_CR13","doi-asserted-by":"publisher","first-page":"1324","DOI":"10.1210\/en.2002-0005","volume":"144","author":"C-R Chen","year":"2003","unstructured":"Chen C-R, Chazenbalk GD, McLachlan SM et al (2003) Targeted restoration of cleavage in a noncleaving thyrotropin receptor demonstrates that cleavage is insufficient to enhance ligand-independent activity. Endocrinology 144:1324\u20131330","journal-title":"Endocrinology"},{"key":"16_CR14","doi-asserted-by":"publisher","first-page":"27459","DOI":"10.1038\/srep27459","volume":"6","author":"J Garc\u00eda-Nafr\u00eda","year":"2016","unstructured":"Garc\u00eda-Nafr\u00eda J, Watson JF, Greger IH (2016) IVA cloning: a single-tube universal cloning system exploiting bacterial in vivo assembly. Sci Rep 6:27459","journal-title":"Sci Rep"},{"key":"16_CR15","doi-asserted-by":"publisher","first-page":"2991","DOI":"10.1038\/s41596-018-0075-9","volume":"13","author":"J Elegheert","year":"2018","unstructured":"Elegheert J, Behiels E, Bishop B et al (2018) Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. Nat Protoc 13:2991\u20133017","journal-title":"Nat Protoc"},{"key":"16_CR16","doi-asserted-by":"publisher","first-page":"13419","DOI":"10.1073\/pnas.212519299","volume":"99","author":"PJ Reeves","year":"2002","unstructured":"Reeves PJ, Callewaert N, Contreras R et al (2002) Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line. Proc Natl Acad Sci U S A 99:13419\u201313424","journal-title":"Proc Natl Acad Sci U S A"},{"key":"16_CR17","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/978-1-4939-3637-3_5","volume":"1432","author":"J Andr\u00e9ll","year":"2016","unstructured":"Andr\u00e9ll J, Edwards PC, Zhang F et al (2016) Generation of tetracycline-inducible mammalian cell lines by flow cytometry for improved overproduction of membrane proteins. Methods Mol Biol 1432:63\u201378","journal-title":"Methods Mol Biol"},{"key":"16_CR18","unstructured":"Thomas JA (2015), Engineering the angiotensin II type 1 receptor for structural studies. Dissertation, University of Cambridge"},{"key":"16_CR19","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/S0301-4622(02)00057-1","volume":"97","author":"HE Davis","year":"2002","unstructured":"Davis HE, Morgan JR, Yarmush ML (2002) Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys Chem 97:159\u2013172","journal-title":"Biophys Chem"}],"container-title":["Methods in Molecular Biology","Heterologous Expression of Membrane Proteins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2368-8_16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T16:22:22Z","timestamp":1656606142000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2368-8_16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"ISBN":["9781071623671","9781071623688"],"references-count":19,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2368-8_16","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"1 July 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}