{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T10:56:09Z","timestamp":1789124169025,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer US","isbn-type":[{"value":"9781071624166","type":"print"},{"value":"9781071624173","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022]]},"DOI":"10.1007\/978-1-0716-2417-3_10","type":"book-chapter","created":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T07:04:04Z","timestamp":1654671844000},"page":"129-153","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cell Viability Assays for Candida auris"],"prefix":"10.1007","author":[{"given":"Jason L.","family":"Brown","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryn","family":"Short","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Ware","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leighann","family":"Sherry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Kean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Ramage","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"issue":"9","key":"10_CR1","doi-asserted-by":"publisher","first-page":"2475","DOI":"10.1128\/AAC.45.9.2475-2479.2001","volume":"45","author":"G Ramage","year":"2001","unstructured":"Ramage G, Vande Walle K, Wickes BL, Lopez-Ribot JL (2001) Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 45(9):2475\u20132479","journal-title":"Antimicrob Agents Chemother"},{"issue":"9","key":"10_CR2","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1038\/nprot.2008.141","volume":"3","author":"CG Pierce","year":"2008","unstructured":"Pierce CG, Uppuluri P, Tristan AR, Wormley FL Jr, Mowat E, Ramage G, Lopez-Ribot JL (2008) A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc 3(9):1494\u20131500","journal-title":"Nat Protoc"},{"key":"10_CR3","first-page":"2287","volume":"44","author":"CG Pierce","year":"2010","unstructured":"Pierce CG, Uppuluri P, Tummala S, Lopez-Ribot JL (2010) A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. J Vis Exp 44:2287","journal-title":"J Vis Exp"},{"key":"10_CR4","volume":"111","author":"AG Dalecki","year":"2016","unstructured":"Dalecki AG, Crawford CL, Wolschendorf F (2016) Targeting biofilm associated staphylococcus aureus using resazurin based drug-susceptibility assay. J Vis Exp 111:53925","journal-title":"J Vis Exp"},{"key":"10_CR5","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.mimet.2013.12.011","volume":"98","author":"F Van den Driessche","year":"2014","unstructured":"Van den Driessche F, Rigole P, Brackman G, Coenye T (2014) Optimization of resazurin-based viability staining for quantification of microbial biofilms. J Microbiol Methods 98:31\u201334","journal-title":"J Microbiol Methods"},{"issue":"4","key":"10_CR6","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1111\/j.2041-1014.2011.00615.x","volume":"26","author":"G Loozen","year":"2011","unstructured":"Loozen G, Boon N, Pauwels M, Quirynen M, Teughels W (2011) Live\/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies. Mol Oral Microbiol 26(4):253\u2013261","journal-title":"Mol Oral Microbiol"},{"issue":"1","key":"10_CR7","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/s00253-012-4524-z","volume":"97","author":"G Agusti","year":"2013","unstructured":"Agusti G, Fittipaldi M, Morato J, Codony F (2013) Viable quantitative PCR for assessing the response of Candida albicans to antifungal treatment. Appl Microbiol Biotechnol 97(1):341\u2013349","journal-title":"Appl Microbiol Biotechnol"},{"issue":"1","key":"10_CR8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/S0167-7012(99)00048-2","volume":"37","author":"L Boulos","year":"1999","unstructured":"Boulos L, Prevost M, Barbeau B, Coallier J, Desjardins R (1999) LIVE\/DEAD BacLight : application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Methods 37(1):77\u201386","journal-title":"J Microbiol Methods"},{"issue":"7","key":"10_CR9","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.1128\/aem.63.7.2897-2905.1997","volume":"63","author":"PJ Millard","year":"1997","unstructured":"Millard PJ, Roth BL, Thi HP, Yue ST, Haugland RP (1997) Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts. Appl Environ Microbiol 63(7):2897\u20132905","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"10_CR10","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1111\/j.1348-0421.2008.00083.x","volume":"53","author":"K Satoh","year":"2009","unstructured":"Satoh K, Makimura K, Hasumi Y, Nishiyama Y, Uchida K, Yamaguchi H (2009) Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital. Microbiol Immunol 53(1):41\u201344","journal-title":"Microbiol Immunol"},{"issue":"2","key":"10_CR11","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1093\/cid\/ciw691","volume":"64","author":"SR Lockhart","year":"2017","unstructured":"Lockhart SR, Etienne KA, Vallabhaneni S, Farooqi J, Chowdhary A, Govender NP, Colombo AL, Calvo B, Cuomo CA, Desjardins CA, Berkow EL, Castanheira M, Magobo RE, Jabeen K, Asghar RJ, Meis JF, Jackson B, Chiller T, Litvintseva AP (2017) Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses. Clin Infect Dis 64(2):134\u2013140","journal-title":"Clin Infect Dis"},{"issue":"4","key":"10_CR12","doi-asserted-by":"publisher","first-page":"e00458","DOI":"10.1128\/mSphere.00458-19","volume":"4","author":"R Kean","year":"2019","unstructured":"Kean R, Ramage G (2019) Combined antifungal resistance and biofilm tolerance: the global threat of Candida auris. mSphere 4(4):e00458\u2013e00419","journal-title":"mSphere"},{"issue":"3","key":"10_CR13","doi-asserted-by":"publisher","first-page":"e02319","DOI":"10.1128\/AAC.02319-17","volume":"62","author":"CL Hager","year":"2018","unstructured":"Hager CL, Larkin EL, Long L, Zohra Abidi F, Shaw KJ, Ghannoum MA (2018) In vitro and in vivo evaluation of the antifungal activity of APX001A\/APX001 against Candida auris. Antimicrob Agents Chemother 62(3):e02319\u2013e02317","journal-title":"Antimicrob Agents Chemother"},{"key":"10_CR14","doi-asserted-by":"publisher","first-page":"83","DOI":"10.3389\/fcimb.2019.00083","volume":"9","author":"HC de Oliveira","year":"2019","unstructured":"de Oliveira HC, Monteiro MC, Rossi SA, Peman J, Ruiz-Gaitan A, Mendes-Giannini MJS, Mellado E, Zaragoza O (2019) Identification of off-patent compounds that present antifungal activity against the emerging fungal pathogen Candida auris. Front Cell Infect Microbiol 9:83","journal-title":"Front Cell Infect Microbiol"},{"issue":"10","key":"10_CR15","doi-asserted-by":"publisher","first-page":"2943","DOI":"10.1093\/jac\/dkz280","volume":"74","author":"SM Rudramurthy","year":"2019","unstructured":"Rudramurthy SM, Colley T, Abdolrasouli A, Ashman J, Dhaliwal M, Kaur H, Armstrong-James D, Strong P, Rapeport G, Schelenz S, Ito K, Chakrabarti A (2019) In vitro antifungal activity of a novel topical triazole PC945 against emerging yeast Candida auris. J Antimicrob Chemother 74(10):2943\u20132949","journal-title":"J Antimicrob Chemother"},{"issue":"8","key":"10_CR16","doi-asserted-by":"publisher","first-page":"e00476","DOI":"10.1128\/AAC.00476-20","volume":"64","author":"LS Arias","year":"2020","unstructured":"Arias LS, Butcher MC, Short B, McKloud E, Delaney C, Kean R, Monteiro DR, Williams C, Ramage G, Brown JL (2020) Chitosan Ameliorates Candida auris Virulence in a Galleria mellonella Infection Model. Antimicrob Agents Chemother 64(8):e00476\u2013e00420","journal-title":"Antimicrob Agents Chemother"},{"key":"10_CR17","volume-title":"Reference method for broth dilution antifungal susceptibility testing of yeasts","author":"CLSI","year":"2008","unstructured":"CLSI (2008) Reference method for broth dilution antifungal susceptibility testing of yeasts, 3rd edn. Clinical and Laboratory Standards Institute (CLSI). Document M27-A3.","edition":"3"},{"key":"10_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioflm.2019.100010","volume":"2","author":"J Allkja","year":"2020","unstructured":"Allkja J, Bjarnsholt T, Coenye T, Cos P, Fallarero A, Harrison J, Lopes S, Oliver A, Pereira M, Ramage G, Shirtliff M, Stoodley P, Webb J, Zaat S, Goeres D (2020) Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates. Biofilms 2:100010","journal-title":"Biofilms"},{"issue":"4","key":"10_CR19","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.jhin.2017.11.015","volume":"98","author":"R Kean","year":"2017","unstructured":"Kean R, Sherry L, Townsend E, McKloud E, Short B, Akinbobola A, Mackay WG, Williams C, Jones BL, Ramage G (2017) Surface disinfection challenges for Candida auris: an in vitro study. J Hosp Infect 98(4):433\u2013436","journal-title":"J Hosp Infect"},{"issue":"3","key":"10_CR20","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1080\/1040841X.2016.1208146","volume":"43","author":"J Azeredo","year":"2017","unstructured":"Azeredo J, Azevedo NF, Briandet R, Cerca N, Coenye T, Costa AR, Desvaux M, Di Bonaventura G, Hebraud M, Jaglic Z, Kacaniova M, Knochel S, Lourenco A, Mergulhao F, Meyer RL, Nychas G, Simoes M, Tresse O, Sternberg C (2017) Critical review on biofilm methods. Crit Rev Microbiol 43(3):313\u2013351","journal-title":"Crit Rev Microbiol"},{"issue":"4","key":"10_CR21","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1111\/j.1532-849X.2009.00561.x","volume":"19","author":"A Jose","year":"2010","unstructured":"Jose A, Coco BJ, Milligan S, Young B, Lappin DF, Bagg J, Murray C, Ramage G (2010) Reducing the incidence of denture stomatitis: are denture cleansers sufficient? J Prosthodont 19(4):252\u2013257","journal-title":"J Prosthodont"},{"issue":"9","key":"10_CR22","doi-asserted-by":"publisher","first-page":"1205","DOI":"10.1099\/jmm.0.47247-0","volume":"56","author":"E Mowat","year":"2007","unstructured":"Mowat E, Butcher J, Lang S, Williams C, Ramage G (2007) Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J Med Microbiol 56(9):1205\u20131212","journal-title":"J Med Microbiol"},{"issue":"6","key":"10_CR23","first-page":"732","volume":"38","author":"AA Miles","year":"1938","unstructured":"Miles AA, Misra SS, Irwin JO (1938) The estimation of the bactericidal power of the blood. J Hyg (Lond) 38(6):732\u2013749","journal-title":"J Hyg (Lond)"},{"key":"10_CR24","doi-asserted-by":"publisher","first-page":"258","DOI":"10.3389\/fmicb.2017.00258","volume":"8","author":"R Kean","year":"2017","unstructured":"Kean R, Rajendran R, Haggarty J, Townsend EM, Short B, Burgess KE, Lang S, Millington O, Mackay WG, Williams C, Ramage G (2017) Candida albicans mycofilms support staphylococcus aureus colonization and enhances miconazole resistance in dual-species interactions. Front Microbiol 8:258","journal-title":"Front Microbiol"},{"key":"10_CR25","doi-asserted-by":"publisher","first-page":"912","DOI":"10.3389\/fmicb.2016.00912","volume":"7","author":"L Sherry","year":"2016","unstructured":"Sherry L, Lappin G, O'Donnell LE, Millhouse E, Millington OR, Bradshaw DJ, Axe AS, Williams C, Nile CJ, Ramage G (2016) Viable compositional analysis of an eleven species oral polymicrobial biofilm. Front Microbiol 7:912","journal-title":"Front Microbiol"},{"issue":"1","key":"10_CR26","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1038\/s41426-018-0187-x","volume":"7","author":"H Yue","year":"2018","unstructured":"Yue H, Bing J, Zheng Q, Zhang Y, Hu T, Du H, Wang H, Huang G (2018) Filamentation in Candida auris, an emerging fungal pathogen of humans: passage through the mammalian body induces a heritable phenotypic switch. Emerg Microbes Infect 7(1):188","journal-title":"Emerg Microbes Infect"},{"issue":"1","key":"10_CR27","first-page":"93","volume":"7","author":"X Wang","year":"2018","unstructured":"Wang X, Bing J, Zheng Q, Zhang F, Liu J, Yue H, Tao L, Du H, Wang Y, Wang H, Huang G (2018) The first isolate of Candida auris in China: clinical and biological aspects. Emerg Microbes Infect 7(1):93","journal-title":"Emerg Microbes Infect"},{"issue":"1","key":"10_CR28","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.jhin.2019.06.006","volume":"103","author":"B Short","year":"2019","unstructured":"Short B, Brown J, Delaney C, Sherry L, Williams C, Ramage G, Kean R (2019) Candida auris exhibits resilient biofilm characteristics in vitro: implications for environmental persistence. J Hosp Infect 103(1):92\u201396","journal-title":"J Hosp Infect"},{"issue":"2","key":"10_CR29","doi-asserted-by":"publisher","first-page":"85","DOI":"10.3390\/jof6020085","volume":"6","author":"LG Cleare","year":"2020","unstructured":"Cleare LG, Li KL, Abuzeid WM, Nacharaju P, Friedman JM, Nosanchuk JD (2020) NO Candida auris: nitric oxide in nanotherapeutics to combat emerging fungal pathogen Candida auris. J Fungi (Basel) 6(2):85","journal-title":"J Fungi (Basel)"},{"issue":"5","key":"10_CR30","doi-asserted-by":"publisher","first-page":"e02396","DOI":"10.1128\/AAC.02396-16","volume":"61","author":"E Larkin","year":"2017","unstructured":"Larkin E, Hager C, Chandra J, Mukherjee PK, Retuerto M, Salem I, Long L, Isham N, Kovanda L, Borroto-Esoda K, Wring S, Angulo D, Ghannoum M (2017) The emerging pathogen Candida auris: growth phenotype, virulence factors, activity of antifungals, and effect of SCY-078, a novel glucan synthesis inhibitor, on growth morphology and biofilm formation. Antimicrob Agents Chemother 61(5):e02396\u2013e02316","journal-title":"Antimicrob Agents Chemother"},{"issue":"2","key":"10_CR31","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.mimet.2009.05.018","volume":"78","author":"BD Essary","year":"2009","unstructured":"Essary BD, Marshall PA (2009) Assessment of FUN-1 vital dye staining: yeast with a block in the vacuolar sorting pathway have impaired ability to form CIVS when stained with FUN-1 fluorescent dye. J Microbiol Methods 78(2):208\u2013212","journal-title":"J Microbiol Methods"},{"issue":"4","key":"10_CR32","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1099\/jmm.0.000237","volume":"65","author":"G Ramage","year":"2016","unstructured":"Ramage G (2016) Comparing apples and oranges: considerations for quantifying candidal biofilms with XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] and the need for standardized testing. J Med Microbiol 65(4):259\u2013260","journal-title":"J Med Microbiol"},{"issue":"2","key":"10_CR33","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.mimet.2006.04.015","volume":"67","author":"A Nocker","year":"2006","unstructured":"Nocker A, Cheung CY, Camper AK (2006) Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J Microbiol Methods 67(2):310\u2013320","journal-title":"J Microbiol Methods"}],"container-title":["Methods in Molecular Biology","Candida auris"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2417-3_10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T18:03:43Z","timestamp":1654711423000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2417-3_10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022]]},"ISBN":["9781071624166","9781071624173"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2417-3_10","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022]]},"assertion":[{"value":"9 June 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}