{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T02:52:11Z","timestamp":1743130331342,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":52,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071626467"},{"type":"electronic","value":"9781071626474"}],"license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-2647-4_9","type":"book-chapter","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T21:02:23Z","timestamp":1664571743000},"page":"115-136","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of FOXP3 DNA Methylation Patterns to Identify Functional FOXP3+ T-Cell Subpopulations"],"prefix":"10.1007","author":[{"given":"Ekaterina","family":"Minskaia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o F.","family":"Lacerda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,10,1]]},"reference":[{"key":"9_CR1","doi-asserted-by":"publisher","unstructured":"Zhang Y, Rohde C, Reinhardt R et al (2009) Non-imprinted allele-specific DNA methylation on human autosomes. Genome Biol 10. https:\/\/doi.org\/10.1186\/gb-2009-10-12-r138","DOI":"10.1186\/gb-2009-10-12-r138"},{"key":"9_CR2","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1038\/ng.286","volume":"41","author":"ZA Kaminsky","year":"2009","unstructured":"Kaminsky ZA, Tang T, Wang S-C et al (2009) DNA methylation profiles in monozygotic and dizygotic twins. Nat Genet 41:240\u2013245. https:\/\/doi.org\/10.1038\/ng.286","journal-title":"Nat Genet"},{"key":"9_CR3","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1002\/bies.950170209","volume":"17","author":"RLP Adams","year":"1995","unstructured":"Adams RLP (1995) Eukaryotic DNA methyltransferases \u2013 structure and function. BioEssays 17:139\u2013145","journal-title":"BioEssays"},{"key":"9_CR4","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1038\/321209a0","volume":"321","author":"AP Bird","year":"1986","unstructured":"Bird AP (1986) CpG-rich islands and the function of DNA methylation. Nature 321:209\u2013213. https:\/\/doi.org\/10.1038\/321209a0","journal-title":"Nature"},{"key":"9_CR5","doi-asserted-by":"publisher","first-page":"147","DOI":"10.4161\/epi.6.2.13640","volume":"6","author":"NP Blackledge","year":"2011","unstructured":"Blackledge NP, Klose RJ (2011) CpG island chromatin: a platform for gene regulation. Epigenetics 6:147\u2013152. https:\/\/doi.org\/10.4161\/epi.6.2.13640","journal-title":"Epigenetics"},{"key":"9_CR6","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1101\/gad.2037511","volume":"25","author":"AM Deaton","year":"2011","unstructured":"Deaton AM, Bird A (2011) CpG islands and the regulation of transcription. Genes Dev 25:1010\u20131022. https:\/\/doi.org\/10.1101\/gad.2037511","journal-title":"Genes Dev"},{"key":"9_CR7","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1038\/nrg887","volume":"3","author":"E Li","year":"2002","unstructured":"Li E (2002) Chromatin modification and epigenetic reprogramming in mammalian development. Nat Rev Genet 3:662\u2013673","journal-title":"Nat Rev Genet"},{"key":"9_CR8","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1038\/nature05913","volume":"447","author":"A Bird","year":"2007","unstructured":"Bird A (2007) Perceptions of epigenetics. Nature 447:396\u2013398","journal-title":"Nature"},{"key":"9_CR9","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/nature07107","volume":"454","author":"A Meissner","year":"2008","unstructured":"Meissner A, Mikkelsen TS, Gu H et al (2008) Genome-scale DNA methylation maps of pluripotent and differentiated cells. Nature 454:766\u2013770. https:\/\/doi.org\/10.1038\/nature07107","journal-title":"Nature"},{"key":"9_CR10","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1016\/j.molcel.2008.05.007","volume":"30","author":"F Mohn","year":"2008","unstructured":"Mohn F, Weber M, Rebhan M et al (2008) Lineage-specific Polycomb targets and De novo DNA methylation define restriction and potential of neuronal progenitors. Mol Cell 30:755\u2013766. https:\/\/doi.org\/10.1016\/j.molcel.2008.05.007","journal-title":"Mol Cell"},{"key":"9_CR11","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/nrg816","volume":"3","author":"PA Jones","year":"2002","unstructured":"Jones PA, Baylin SB (2002) The fundamental role of epigenetic events in cancer. Nat Rev Genet 3:415\u2013428","journal-title":"Nat Rev Genet"},{"key":"9_CR12","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1038\/nbt.2487","volume":"31","author":"Y Liu","year":"2013","unstructured":"Liu Y, Aryee MJ, Padyukov L et al (2013) Epigenome-wide association data implicate DNA methylation as an intermediary of genetic risk in rheumatoid arthritis. Nat Biotechnol 31:142\u2013147. https:\/\/doi.org\/10.1038\/nbt.2487","journal-title":"Nat Biotechnol"},{"key":"9_CR13","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1177\/1352458513516529","volume":"20","author":"MC Graves","year":"2014","unstructured":"Graves MC, Benton M, Lea RA et al (2014) Methylation differences at the HLA-DRB1 locus in CD4+ T-cells are associated with multiple sclerosis. Mult Scler J 20:1033\u20131041. https:\/\/doi.org\/10.1177\/1352458513516529","journal-title":"Mult Scler J"},{"key":"9_CR14","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1093\/nar\/6.6.2125","volume":"6","author":"H Cedar","year":"1979","unstructured":"Cedar H, Solage A, Glaser G, Razin A (1979) Direct detection of methylated cytosine in DNA by use of the restriction enzyme Mspl. Nucleic Acids Res 6:2125\u20132132. https:\/\/doi.org\/10.1093\/nar\/6.6.2125","journal-title":"Nucleic Acids Res"},{"key":"9_CR15","doi-asserted-by":"publisher","first-page":"9821","DOI":"10.1073\/pnas.93.18.9821","volume":"93","author":"JG Herman","year":"1996","unstructured":"Herman JG, Graff JR, My\u00f6h\u00e4nen S et al (1996) Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93:9821\u20139826. https:\/\/doi.org\/10.1073\/pnas.93.18.9821","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR16","doi-asserted-by":"publisher","first-page":"2532","DOI":"10.1093\/nar\/25.12.2532","volume":"25","author":"Z Xiong","year":"1997","unstructured":"Xiong Z, Laird PW (1997) COBRA: a sensitive and quantitative DNA methylation assay. Nucleic Acids Res 25:2532\u20132534. https:\/\/doi.org\/10.1093\/nar\/25.12.2532","journal-title":"Nucleic Acids Res"},{"key":"9_CR17","doi-asserted-by":"publisher","first-page":"2529","DOI":"10.1093\/nar\/25.12.2529","volume":"25","author":"ML Gonzalgo","year":"1997","unstructured":"Gonzalgo ML, Jones PA (1997) Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE). Nucleic Acids Res 25:2529\u20132531. https:\/\/doi.org\/10.1093\/nar\/25.12.2529","journal-title":"Nucleic Acids Res"},{"key":"9_CR18","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1002\/(SICI)1098-1004(199910)14:4<289::AID-HUMU3>3.0.CO;2-A","volume":"14","author":"T Bianco","year":"1999","unstructured":"Bianco T, Hussey D, Dobrovic A (1999) Methylation-sensitive, single-strand conformation analysis (MS-SSCA): a rapid method to screen for and analyze methylation. Hum Mutat 14:289\u2013293. https:\/\/doi.org\/10.1002\/(SICI)1098-1004(199910)14:4<289::AID-HUMU3>3.0.CO;2-A","journal-title":"Hum Mutat"},{"key":"9_CR19","doi-asserted-by":"publisher","unstructured":"Wojdacz TK, Dobrovic A (2007) Methylation-sensitive high resolution melting (MS-HRM): a new approach for sensitive and high-throughput assessment of methylation. Nucleic Acids Res 35. https:\/\/doi.org\/10.1093\/nar\/gkm013","DOI":"10.1093\/nar\/gkm013"},{"key":"9_CR20","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1016\/j.cell.2006.08.003","volume":"126","author":"X Zhang","year":"2006","unstructured":"Zhang X, Yazaki J, Sundaresan A et al (2006) Genome-wide high-resolution mapping and functional analysis of DNA methylation in Arabidopsis. Cell 126:1189\u20131201. https:\/\/doi.org\/10.1016\/j.cell.2006.08.003","journal-title":"Cell"},{"key":"9_CR21","doi-asserted-by":"publisher","first-page":"146","DOI":"10.2144\/03351md01","volume":"35","author":"S Colella","year":"2003","unstructured":"Colella S, Shen L, Baggerly KA et al (2003) Sensitive and quantitative universal pyrosequencing\u2122 methylation analysis of CpG sites. BioTechniques 35:146\u2013150. https:\/\/doi.org\/10.2144\/03351md01","journal-title":"BioTechniques"},{"key":"9_CR22","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.ab.2004.05.007","volume":"333","author":"JM Dupont","year":"2004","unstructured":"Dupont JM, Tost J, Jammes H, Gut IG (2004) De novo quantitative bisulfite sequencing using the pyrosequencing technology. Anal Biochem 333:119\u2013127. https:\/\/doi.org\/10.1016\/j.ab.2004.05.007","journal-title":"Anal Biochem"},{"key":"9_CR23","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1101\/gr.7073008","volume":"18","author":"J Reinders","year":"2008","unstructured":"Reinders J, Vivier CD, Theiler G et al (2008) Genome-wide, high-resolution DNA methylation profiling using bisulfite-mediated cytosine conversion. Genome Res 18:469\u2013476. https:\/\/doi.org\/10.1101\/gr.7073008","journal-title":"Genome Res"},{"key":"9_CR24","doi-asserted-by":"publisher","first-page":"2990","DOI":"10.1002\/1522-2683(20000801)21:14<2990::AID-ELPS2990>3.0.CO;2-I","volume":"21","author":"MF Fraga","year":"2000","unstructured":"Fraga MF, Rodr\u00edguez R, Ca\u00f1al MJ (2000) Rapid quantification of DNA methylation by high performance capillary electrophoresis. Electrophoresis 21:2990\u20132994. https:\/\/doi.org\/10.1002\/1522-2683(20000801)21:14<2990::AID-ELPS2990>3.0.CO;2-I","journal-title":"Electrophoresis"},{"key":"9_CR25","doi-asserted-by":"publisher","unstructured":"Schatz P, Distler J, Berlin K, Schuster M (2006) Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection. Nucleic Acids Res 34. https:\/\/doi.org\/10.1093\/nar\/gkl218","DOI":"10.1093\/nar\/gkl218"},{"key":"9_CR26","doi-asserted-by":"publisher","first-page":"5868","DOI":"10.1093\/nar\/gki901","volume":"33","author":"A Meissner","year":"2005","unstructured":"Meissner A, Gnirke A, Bell GW et al (2005) Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis. Nucleic Acids Res 33:5868\u20135877. https:\/\/doi.org\/10.1093\/nar\/gki901","journal-title":"Nucleic Acids Res"},{"key":"9_CR27","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1038\/nrg3000","volume":"12","author":"VK Rakyan","year":"2011","unstructured":"Rakyan VK, Down TA, Balding DJ, Beck S (2011) Epigenome-wide association studies for common human diseases. Nat Rev Genet 12:529\u2013541","journal-title":"Nat Rev Genet"},{"key":"9_CR28","doi-asserted-by":"publisher","first-page":"1827","DOI":"10.1073\/pnas.89.5.1827","volume":"89","author":"M Frommer","year":"1992","unstructured":"Frommer M, McDonald LE, Millar DS et al (1992) A genomic sequencing protocol that yields a positive display of 5- methylcytosine residues in individual DNA strands. Proc Natl Acad Sci U S A 89:1827\u20131831. https:\/\/doi.org\/10.1073\/pnas.89.5.1827","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR29","doi-asserted-by":"publisher","first-page":"8392","DOI":"10.4049\/jimmunol.175.12.8392","volume":"175","author":"M Miyara","year":"2005","unstructured":"Miyara M, Amoura Z, Parizot C et al (2005) Global natural regulatory T cell depletion in active systemic lupus erythematosus. J Immunol 175:8392\u20138400","journal-title":"J Immunol"},{"key":"9_CR30","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.cell.2008.05.009","volume":"133","author":"S Sakaguchi","year":"2008","unstructured":"Sakaguchi S, Yamaguchi T, Nomura T, Ono M (2008) Regulatory T cells and immune tolerance. Cell 133:775\u2013787. https:\/\/doi.org\/10.1016\/j.cell.2008.05.009","journal-title":"Cell"},{"key":"9_CR31","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1093\/intimm\/dxm014","volume":"19","author":"SE Allan","year":"2007","unstructured":"Allan SE, Crome SQ, Crellin NK et al (2007) Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production. Int Immunol 19:345\u2013354. https:\/\/doi.org\/10.1093\/intimm\/dxm014","journal-title":"Int Immunol"},{"key":"9_CR32","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1002\/eji.200636435","volume":"37","author":"J Wang","year":"2007","unstructured":"Wang J, Ioan-Facsinay A, van der Voort EIH et al (2007) Transient expression of FOXP3 in human activated nonregulatory CD4+ T cells. Eur J Immunol 37:129\u2013138. https:\/\/doi.org\/10.1002\/eji.200636435","journal-title":"Eur J Immunol"},{"key":"9_CR33","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/j.immuni.2009.03.019","volume":"30","author":"M Miyara","year":"2009","unstructured":"Miyara M, Yoshioka Y, Kitoh A et al (2009) Functional delineation and differentiation dynamics of human CD4+ T cells expressing the FoxP3 transcription factor. Immunity 30:899\u2013911. https:\/\/doi.org\/10.1016\/j.immuni.2009.03.019","journal-title":"Immunity"},{"key":"9_CR34","doi-asserted-by":"publisher","first-page":"7225","DOI":"10.1073\/pnas.1508224112","volume":"112","author":"M Miyara","year":"2015","unstructured":"Miyara M, Chader D, Sage E et al (2015) Sialyl Lewis x (CD15s) identifies highly differentiated and most suppressive FOXP3high regulatory T cells in humans. Proc Natl Acad Sci U S A 112:7225\u20137230. https:\/\/doi.org\/10.1073\/pnas.1508224112","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR35","doi-asserted-by":"publisher","unstructured":"Minskaia E, Saraiva BC, Soares MMV et al (2018) Molecular markers distinguishing T cell subtypes with TSDR strand-bias methylation. Front Immunol 9. https:\/\/doi.org\/10.3389\/fimmu.2018.02540","DOI":"10.3389\/fimmu.2018.02540"},{"key":"9_CR36","doi-asserted-by":"publisher","first-page":"2378","DOI":"10.1002\/eji.200737594","volume":"37","author":"U Baron","year":"2007","unstructured":"Baron U, Floess S, Wieczorek G et al (2007) DNA demethylation in the human FOXP3 locus discriminates regulatory T cells from activated FOXP3+ conventional T cells. Eur J Immunol 37:2378\u20132389. https:\/\/doi.org\/10.1002\/eji.200737594","journal-title":"Eur J Immunol"},{"key":"9_CR37","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1038\/nature08750","volume":"463","author":"Y Zheng","year":"2010","unstructured":"Zheng Y, Josefowicz S, Chaudhry A et al (2010) Role of conserved non-coding DNA elements in the Foxp3 gene in regulatory T-cell fate. Nature 463:808\u2013812. https:\/\/doi.org\/10.1038\/nature08750","journal-title":"Nature"},{"key":"9_CR38","doi-asserted-by":"publisher","first-page":"E3472","DOI":"10.1073\/pnas.1700946114","volume":"114","author":"D Zemmour","year":"2017","unstructured":"Zemmour D, Pratama A, Loughhead SM et al (2017) Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. Proc Natl Acad Sci U S A 114:E3472\u2013E3480. https:\/\/doi.org\/10.1073\/pnas.1700946114","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR39","doi-asserted-by":"publisher","first-page":"5289","DOI":"10.1073\/pnas.1312717110","volume":"111","author":"H Morikawa","year":"2014","unstructured":"Morikawa H, Ohkura N, Vandenbon A et al (2014) Differential roles of epigenetic changes and Foxp3 expression in regulatory T cell-specific transcriptional regulation. Proc Natl Acad Sci U S A 111:5289\u20135294. https:\/\/doi.org\/10.1073\/pnas.1312717110","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR40","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1016\/j.immuni.2013.08.006","volume":"39","author":"Z Chen","year":"2013","unstructured":"Chen Z, Barbi J, Bu S et al (2013) The ubiquitin ligase stub1 negatively modulates regulatory T cell suppressive activity by promoting degradation of the transcription factor Foxp3. Immunity 39:272\u2013285. https:\/\/doi.org\/10.1016\/j.immuni.2013.08.006","journal-title":"Immunity"},{"key":"9_CR41","doi-asserted-by":"publisher","first-page":"2968","DOI":"10.1002\/eji.201444453","volume":"44","author":"A Kennedy","year":"2014","unstructured":"Kennedy A, Schmidt EM, Cribbs AP et al (2014) A novel upstream enhancer of FOXP3, sensitive to methylation-induced silencing, exhibits dysregulated methylation in rheumatoid arthritis Treg cells. Eur J Immunol 44:2968\u20132978. https:\/\/doi.org\/10.1002\/eji.201444453","journal-title":"Eur J Immunol"},{"key":"9_CR42","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1038\/ng1158","volume":"34","author":"T Hashimshony","year":"2003","unstructured":"Hashimshony T, Zhang J, Keshet I et al (2003) The role of DNA methylation in setting up chromatin structure during development. Nat Genet 34:187\u2013192. https:\/\/doi.org\/10.1038\/ng1158","journal-title":"Nat Genet"},{"key":"9_CR43","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1182\/blood-2013-02-486944","volume":"123","author":"C Schmidl","year":"2014","unstructured":"Schmidl C, Hansmann L, Lassmann T et al (2014) The enhancer and promoter landscape of human regulatory and conventional T-cell subpopulations. Blood 123:68\u201379. https:\/\/doi.org\/10.1182\/blood-2013-02-486944","journal-title":"Blood"},{"key":"9_CR44","doi-asserted-by":"publisher","first-page":"1654","DOI":"10.1002\/eji.200838105","volume":"38","author":"JK Polansky","year":"2008","unstructured":"Polansky JK, Kretschmer K, Freyer J et al (2008) DNA methylation controls Foxp3 gene expression. Eur J Immunol 38:1654\u20131663. https:\/\/doi.org\/10.1002\/eji.200838105","journal-title":"Eur J Immunol"},{"key":"9_CR45","doi-asserted-by":"publisher","first-page":"949","DOI":"10.1016\/j.immuni.2013.10.016","volume":"39","author":"SL Bailey-Bucktrout","year":"2013","unstructured":"Bailey-Bucktrout SL, Martinez-Llordella M, Zhou X et al (2013) Self-antigen-driven activation induces instability of regulatory T cells during an inflammatory autoimmune response. Immunity 39:949\u2013962. https:\/\/doi.org\/10.1016\/j.immuni.2013.10.016","journal-title":"Immunity"},{"key":"9_CR46","doi-asserted-by":"publisher","first-page":"2699","DOI":"10.4049\/jimmunol.1400599","volume":"193","author":"D Bending","year":"2014","unstructured":"Bending D, Pesenacker AM, Ursu S et al (2014) Hypomethylation at the regulatory T cell\u2013specific demethylated region in CD25 hi T cells is decoupled from FOXP3 expression at the inflamed site in childhood arthritis. J Immunol 193:2699\u20132708. https:\/\/doi.org\/10.4049\/jimmunol.1400599","journal-title":"J Immunol"},{"key":"9_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1371\/journal.pone.0049962","volume":"7","author":"R Tatura","year":"2012","unstructured":"Tatura R, Zeschnigk M, Adamzik M et al (2012) Quantification of regulatory T cells in septic patients by real-time PCR-based methylation assay and flow cytometry. PLoS One 7:1\u20139. https:\/\/doi.org\/10.1371\/journal.pone.0049962","journal-title":"PLoS One"},{"key":"9_CR48","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1476-4598-13-153","volume":"13","author":"C Zhuo","year":"2014","unstructured":"Zhuo C, Li Z, Xu Y et al (2014) Higher FOXP3-TSDR demethylation rates in adjacent normal tissues in patients with colon cancer were associated with worse survival. Mol Cancer 13:1\u201313. https:\/\/doi.org\/10.1186\/1476-4598-13-153","journal-title":"Mol Cancer"},{"key":"9_CR49","doi-asserted-by":"publisher","first-page":"3317","DOI":"10.4049\/jimmunol.1002502","volume":"186","author":"CM Baecher-Allan","year":"2011","unstructured":"Baecher-Allan CM, Costantino CM, Cvetanovich GL et al (2011) CD2 Costimulation reveals defective activity by human CD4 + CD25 hi regulatory cells in patients with multiple sclerosis. J Immunol 186:3317\u20133326. https:\/\/doi.org\/10.4049\/jimmunol.1002502","journal-title":"J Immunol"},{"key":"9_CR50","doi-asserted-by":"publisher","first-page":"1960","DOI":"10.1002\/ijc.26198","volume":"130","author":"S Lucas","year":"2012","unstructured":"Lucas S, Van Baren N, De Smet C, Coulie PG (2012) Demethylation of the FOXP3 gene in human melanoma cells precludes the use of this epigenetic mark for quantification of Tregs in unseparated melanoma samples. Int J Cancer 130:1960\u20131966. https:\/\/doi.org\/10.1002\/ijc.26198","journal-title":"Int J Cancer"},{"key":"9_CR51","doi-asserted-by":"publisher","first-page":"16894","DOI":"10.1073\/pnas.0607926103","volume":"103","author":"GY Chen","year":"2006","unstructured":"Chen GY, Osada H, Santamaria-Babi LF, Kannagi R (2006) Interaction of GATA-3\/T-bet transcription factors regulates expression of sialil Lewis X homing receptors on Th1\/Th2 lymphocytes. Proc Natl Acad Sci U S A 103:16894\u201316899. https:\/\/doi.org\/10.1073\/pnas.0607926103","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR52","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1002\/stem.3185","volume":"38","author":"RI Azevedo","year":"2020","unstructured":"Azevedo RI, Minskaia E, Fernandes-Platzgummer A et al (2020) Mesenchymal stromal cells induce regulatory T cells via epigenetic conversion of human conventional CD4 T cells in vitro. Stem Cells 38:1007\u20131019. https:\/\/doi.org\/10.1002\/stem.3185","journal-title":"Stem Cells"}],"container-title":["Methods in Molecular Biology","Regulatory T-Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2647-4_9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T21:03:23Z","timestamp":1664571803000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2647-4_9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,1]]},"ISBN":["9781071626467","9781071626474"],"references-count":52,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2647-4_9","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2022,10,1]]},"assertion":[{"value":"1 October 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}