{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:47:34Z","timestamp":1792730854060,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":22,"publisher":"Springer US","isbn-type":[{"value":"9781071626740","type":"print"},{"value":"9781071626757","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T00:00:00Z","timestamp":1664064000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T00:00:00Z","timestamp":1664064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-2675-7_5","type":"book-chapter","created":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T08:18:38Z","timestamp":1664007518000},"page":"53-62","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Labeling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis"],"prefix":"10.1007","author":[{"given":"Patrizia","family":"Vaghi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Oldani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Fulghieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Pollara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enza Maria","family":"Valente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Sottile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,9,25]]},"reference":[{"issue":"5411","key":"5_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1126\/science","volume":"284","author":"MF Pittenger","year":"1999","unstructured":"Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284(5411):143\u2013147. https:\/\/doi.org\/10.1126\/science","journal-title":"Science"},{"key":"5_CR2","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1038\/nrendo.2014.234","volume":"11","author":"W Grayson","year":"2015","unstructured":"Grayson W, Bunnell BA, Martin E, Frazier T, Hung BP, Gimble JM (2015) Stromal cells and stem cells in clinical bone regeneration. Nat Rev Endocrinol 11:140\u2013150. https:\/\/doi.org\/10.1038\/nrendo.2014.234","journal-title":"Nat Rev Endocrinol"},{"key":"5_CR3","doi-asserted-by":"publisher","first-page":"1128","DOI":"10.1038\/cdd.2015.168","volume":"23","author":"Q Chen","year":"2016","unstructured":"Chen Q, Shou P, Zheng C, Jiang M, Cao G, Yang Q et al (2016) Fate decision of mesenchymal stem cells: adipocytes or osteoblasts? Cell Death Differ 23:1128\u20131139. https:\/\/doi.org\/10.1038\/cdd.2015.168","journal-title":"Cell Death Differ"},{"key":"5_CR4","doi-asserted-by":"publisher","first-page":"R165","DOI":"10.1530\/EJE-18-0182","volume":"179","author":"T Rharass","year":"2018","unstructured":"Rharass T, Lucas S (2018) Mechanisms in endocrinology: bone marrow adiposity and bone, a bad romance? Eur J Endocrinol 179:R165\u2013R182. https:\/\/doi.org\/10.1530\/EJE-18-0182","journal-title":"Eur J Endocrinol"},{"key":"5_CR5","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.bone.2019.03.041","volume":"123","author":"JO Nehlin","year":"2019","unstructured":"Nehlin JO, Jafari A, Tencerova M, Kassem M (2019) Aging and lineage allocation changes of bone marrow skeletal (stromal) stem cells. Bone 123:265\u2013273. https:\/\/doi.org\/10.1016\/j.bone.2019.03.041","journal-title":"Bone"},{"key":"5_CR6","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1007\/s00018-008-8429-z","volume":"66","author":"S Muruganandan","year":"2009","unstructured":"Muruganandan S, Roman AA, Sinal CJ (2009) Adipocyte differentiation of bone marrow-derived mesenchymal stem cells: cross talk with the osteoblastogenic program. Cell Mol Life Sci 66:236\u2013253. https:\/\/doi.org\/10.1007\/s00018-008-8429-z","journal-title":"Cell Mol Life Sci"},{"key":"5_CR7","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.actbio.2018.06.020","volume":"76","author":"SL Vega","year":"2018","unstructured":"Vega SL, Arvind V, Mishra P, Kohn J, Murthy NS, Moghe PV (2018) Substrate micropatterns produced by polymer demixing regulate focal adhesions, actin anisotropy, and lineage differentiation of stem cells. Acta Biomater 76:21\u201328. https:\/\/doi.org\/10.1016\/j.actbio.2018.06.020","journal-title":"Acta Biomater"},{"issue":"2","key":"5_CR8","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1002\/ajpa.1330290211","volume":"29","author":"HM Myers","year":"1968","unstructured":"Myers HM (1968) Alizarin and tetracycline binding by bone mineral. Am J Phys Anthropol 29(2):179\u2013182. https:\/\/doi.org\/10.1002\/ajpa.1330290211","journal-title":"Am J Phys Anthropol"},{"issue":"2","key":"5_CR9","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1177\/17.2.110","volume":"17","author":"H Puchtler","year":"1969","unstructured":"Puchtler H, Meloan SN, Terry MS (1969) On the history and mechanism of Alizarin and Alizarin Red S stains for calcium. J Histochem Cytochem 17(2):110\u2013124. https:\/\/doi.org\/10.1177\/17.2.110","journal-title":"J Histochem Cytochem"},{"key":"5_CR10","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1007\/BF00495978","volume":"128","author":"CE Bill","year":"1974","unstructured":"Bill CE, Eisenberg H, Pallante SL (1974) Complexes of organic acids with calcium phosphate: the Von Kossa stain as a clue to the composition of bone mineral. Johns Hopkins Med J 128:194. https:\/\/doi.org\/10.1007\/BF00495978","journal-title":"Johns Hopkins Med J"},{"issue":"2","key":"5_CR11","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1089\/153623003322234759","volume":"5","author":"V Sottile","year":"2003","unstructured":"Sottile V, Thomson A, McWhir J (2003) In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 5(2):149\u2013155. https:\/\/doi.org\/10.1089\/153623003322234759","journal-title":"Cloning Stem Cells"},{"issue":"20","key":"5_CR12","doi-asserted-by":"publisher","first-page":"5051","DOI":"10.3390\/ijms20205051","volume":"20","author":"A Popov","year":"2019","unstructured":"Popov A, Scotchford C, Grant D, Sottile V (2019) Impact of serum source on human mesenchymal stem cell osteogenic differentiation in culture. Int J Mol Sci 20(20):5051. https:\/\/doi.org\/10.3390\/ijms20205051","journal-title":"Int J Mol Sci"},{"key":"5_CR13","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.1136\/jcp.35.11.1278","volume":"35","author":"J McClure","year":"1982","unstructured":"McClure J (1982) Demonstration of calcification fronts by in vivo and in vitro tetracycline labelling. J Clin Pathol 35:1278. https:\/\/doi.org\/10.1136\/jcp.35.11.1278","journal-title":"J Clin Pathol"},{"key":"5_CR14","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1111\/j.1469-7580.2010.01237.x","volume":"217","author":"C Pautke","year":"2010","unstructured":"Pautke C, Vogt S, Kreutzer K, Haczek C, Wexel G, Kolk A et al (2010) Characterization of eight different tetracyclines: advances in fluorescence bone labeling. J Anat 217:76. https:\/\/doi.org\/10.1111\/j.1469-7580.2010.01237.x","journal-title":"J Anat"},{"issue":"3","key":"5_CR15","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1089\/ten.TEC.2017.0400","volume":"24","author":"L Macri-Pellizzeri","year":"2018","unstructured":"Macri-Pellizzeri L, De Melo N, Ahmed I, Grant D, Scammell B, Sottile V (2018) Live quantitative monitoring of mineral deposition in stem cells using tetracycline hydrochloride. Tissue Eng Part C Methods 24(3):171\u2013178. https:\/\/doi.org\/10.1089\/ten.TEC.2017.0400","journal-title":"Tissue Eng Part C Methods"},{"issue":"5","key":"5_CR16","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/s8756-3282(02)00674-9","volume":"30","author":"V Sottile","year":"2002","unstructured":"Sottile V, Halleux C, Bassilana F, Keller H, Seuwen K (2002) Stem cell characteristics of human trabecular bone-derived cells. Bone 30(5):699\u2013704. https:\/\/doi.org\/10.1016\/s8756-3282(02)00674-9","journal-title":"Bone"},{"issue":"35","key":"5_CR17","doi-asserted-by":"publisher","first-page":"5280","DOI":"10.1016\/j.biomaterials.2007.08.017","volume":"28","author":"JR Mauney","year":"2007","unstructured":"Mauney JR, Nguyen T, Gillen K, Kirker-Head C, Gimble JM, Kaplan DL (2007) Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds. Biomaterials 28(35):5280\u20135290. https:\/\/doi.org\/10.1016\/j.biomaterials.2007.08.017","journal-title":"Biomaterials"},{"issue":"1","key":"5_CR18","doi-asserted-by":"publisher","first-page":"3592","DOI":"10.1038\/s41467-018-05911-0","volume":"9","author":"RK Zwick","year":"2018","unstructured":"Zwick RK, Rudolph MC, Shook BA, Holtrup B, Roth E, Lei V et al (2018) Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation. Nat Commun 9(1):3592. https:\/\/doi.org\/10.1038\/s41467-018-05911-0","journal-title":"Nat Commun"},{"issue":"17","key":"5_CR19","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1912","volume":"6","author":"B Qiu","year":"2016","unstructured":"Qiu B, Simon MC (2016) BODIPY 493\/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio Protoc 6(17):e1912. https:\/\/doi.org\/10.21769\/BioProtoc.1912","journal-title":"Bio Protoc"},{"issue":"3","key":"5_CR20","doi-asserted-by":"publisher","first-page":"48","DOI":"10.3390\/biomimetics4030048","volume":"4","author":"N De Melo","year":"2019","unstructured":"De Melo N, McGinlay S, Markus R, Macri-Pellizzeri L, Symonds ME, Ahmed I et al (2019) Live simultaneous monitoring of mineral deposition and lipid accumulation in differentiating stem cells. Biomimetics (Basel) 4(3):48. https:\/\/doi.org\/10.3390\/biomimetics4030048","journal-title":"Biomimetics (Basel)"},{"issue":"7","key":"5_CR21","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","volume":"9","author":"CA Schneider","year":"2012","unstructured":"Schneider CA, Rasband WS, Eliceiri KW (2012) NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9(7):671\u2013675. https:\/\/doi.org\/10.1038\/nmeth.2089","journal-title":"Nat Methods"},{"issue":"8","key":"5_CR22","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1101\/pdb.prot5648","volume":"2011","author":"B Chazotte","year":"2011","unstructured":"Chazotte B (2011) Labeling mitochondria with MitoTracker dyes. Cold Spring Harb Protoc 2011(8):990\u2013992. https:\/\/doi.org\/10.1101\/pdb.prot5648","journal-title":"Cold Spring Harb Protoc"}],"container-title":["Methods in Molecular Biology","Histochemistry of Single Molecules"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2675-7_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T08:22:20Z","timestamp":1664007740000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2675-7_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,25]]},"ISBN":["9781071626740","9781071626757"],"references-count":22,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2675-7_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,25]]},"assertion":[{"value":"25 September 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}