{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T18:23:02Z","timestamp":1776190982082,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":20,"publisher":"Springer US","isbn-type":[{"value":"9781071627396","type":"print"},{"value":"9781071627402","type":"electronic"}],"license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-2740-2_7","type":"book-chapter","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T10:38:56Z","timestamp":1668422336000},"page":"131-149","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A Simplified Approach to Evaluating T Cell Development by Flow Cytometry"],"prefix":"10.1007","author":[{"given":"Jan Y. M.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul E.","family":"Love","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"7_CR1","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1146\/annurev.immunol.14.1.73","volume":"14","author":"G Anderson","year":"1996","unstructured":"Anderson G, Moore NC, Owen JJ, Jenkinson EJ (1996) Cellular interactions in thymocyte development. Annu Rev Immunol 14:73\u201399","journal-title":"Annu Rev Immunol"},{"issue":"11","key":"7_CR2","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1038\/nri1223","volume":"3","author":"HT Petrie","year":"2003","unstructured":"Petrie HT (2003) Cell migration and the control of post-natal T-cell lymphopoiesis in the thymus. Nat Rev Immunol 3(11):859\u2013866","journal-title":"Nat Rev Immunol"},{"key":"7_CR3","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1146\/annurev.cellbio.17.1.387","volume":"17","author":"IL Weissman","year":"2001","unstructured":"Weissman IL, Anderson DJ, Gage F (2001) Stem and progenitor cells: origins, phenotypes, lineage commitments, and transdifferentiations. Annu Rev Cell Dev Biol 17:387\u2013403","journal-title":"Annu Rev Cell Dev Biol"},{"issue":"10","key":"7_CR4","doi-asserted-by":"crossref","first-page":"4244","DOI":"10.4049\/jimmunol.150.10.4244","volume":"150","author":"DI Godfrey","year":"1993","unstructured":"Godfrey DI, Kennedy J, Suda T, Zlotnik A (1993) A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J Immunol 150(10):4244\u20134252","journal-title":"J Immunol"},{"key":"7_CR5","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1146\/annurev.immunol.14.1.29","volume":"14","author":"K Shortman","year":"1996","unstructured":"Shortman K, Wu L (1996) Early T lymphocyte progenitors. Annu Rev Immunol 14:29\u201347","journal-title":"Annu Rev Immunol"},{"key":"7_CR6","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1111\/j.1749-6632.1995.tb26648.x","volume":"766","author":"H Von Boehmer","year":"1995","unstructured":"Von Boehmer H (1995) Control of T-cell development by the pre-T and alpha beta T-cell receptor. Ann N Y Acad Sci 766:52\u201361","journal-title":"Ann N Y Acad Sci"},{"issue":"5","key":"7_CR7","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1016\/S1044-5323(02)00064-7","volume":"14","author":"AM Michie","year":"2002","unstructured":"Michie AM, Zuniga-Pflucker JC (2002) Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Semin Immunol 14(5):311\u2013323","journal-title":"Semin Immunol"},{"key":"7_CR8","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1146\/annurev.iy.13.040195.000521","volume":"13","author":"SC Jameson","year":"1995","unstructured":"Jameson SC, Hogquist KA, Bevan MJ (1995) Positive selection of thymocytes. Annu Rev Immunol 13:93\u2013126","journal-title":"Annu Rev Immunol"},{"key":"7_CR9","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1146\/annurev.immunol.17.1.829","volume":"17","author":"E Sebzda","year":"1999","unstructured":"Sebzda E et al (1999) Selection of the T cell repertoire. Annu Rev Immunol 17:829\u2013874","journal-title":"Annu Rev Immunol"},{"key":"7_CR10","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1146\/annurev.immunol.20.100101.151926","volume":"20","author":"J Sprent","year":"2002","unstructured":"Sprent J, Surh CD (2002) T cell memory. Annu Rev Immunol 20:551\u2013579","journal-title":"Annu Rev Immunol"},{"key":"7_CR11","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1146\/annurev.immunol.25.022106.141711","volume":"25","author":"A Bendelac","year":"2007","unstructured":"Bendelac A, Savage PB, Teyton L (2007) The biology of NKT cells. Annu Rev Immunol 25:297\u2013336","journal-title":"Annu Rev Immunol"},{"key":"7_CR12","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1146\/annurev.immunol.21.120601.141122","volume":"22","author":"S Sakaguchi","year":"2004","unstructured":"Sakaguchi S (2004) Naturally arising CD4+ regulatory t cells for immunologic self-tolerance and negative control of immune responses. Annu Rev Immunol 22:531\u2013562","journal-title":"Annu Rev Immunol"},{"key":"7_CR13","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1146\/annurev.immunol.18.1.975","volume":"18","author":"AC Hayday","year":"2000","unstructured":"Hayday AC (2000) [gamma][delta] cells: a right time and a right place for a conserved third way of protection. Annu Rev Immunol 18:975\u20131026","journal-title":"Annu Rev Immunol"},{"key":"7_CR14","doi-asserted-by":"crossref","unstructured":"Reeves JP, Reeves PA (1991) Removal of lymphoid organs. Curr Protoc Immunol:1.9.1\u20131.9.3","DOI":"10.1002\/0471142735.im0109s01"},{"key":"7_CR15","unstructured":"\u201cFixable Viability Dyes for Flow Cytometry\u201d on Thermo-Fisher website. https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cell-analysis\/flow-cytometry\/flow-cytometry-assays-reagents\/cell-viability-assays-flow-cytometry\/fixable-viability-dyes-flow-cytometry.html?gclid=EAIaIQobChMI7uCrmc7U6AIV0oCfCh2C_wciEAAYASAAEgLoafD_BwE&s_kwcid=AL!3652!3!219985193477!p!!g!!fixable%20viability%20dye&ef_id=EAIaIQobChMI7uCrmc7U6AIV0oCfCh2C_wciEAAYASAAEgLoafD_BwE:G:s&s_kwcid=AL!3652!3!219985193477!p!!g!!fixable%20viability%20dye?cid=bid_pca_frg_r01_co_cp1359_pjt0000_bid00000_0se_gaw_nt_pur_con#prd"},{"key":"7_CR16","doi-asserted-by":"crossref","unstructured":"Darzynkiewicz Z, Juan G, Bedner E (1999) Determining cell cycle stages by flow cytometry. Curr Protoc Cell Biol:8.4.1\u20138.4.18","DOI":"10.1002\/0471143030.cb0804s01"},{"key":"7_CR17","unstructured":"BestProtocols: Staining Intracellular Antigens for Flow Cytometry https:\/\/www.thermofisher.com\/us\/en\/home\/references\/protocols\/cell-and-tissue-analysis\/protocols\/staining-intracellular-antigens-flow-cytometry.html"},{"key":"7_CR18","unstructured":"BD Biosciences Spectrum Viewer. https:\/\/www.bdbiosciences.com\/en-us\/applications\/research-applications\/multicolor-flow-cytometry\/product-selection-tools\/spectrum-viewer"},{"key":"7_CR19","unstructured":"BioLegend Spectrum Viewer. https:\/\/www.biolegend.com\/en-us\/spectra-analyzer"},{"key":"7_CR20","unstructured":"Thermo-Fisher Spectrum Viewer. https:\/\/www.thermofisher.com\/order\/spectra-viewer"}],"container-title":["Methods in Molecular Biology","T-Cell Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2740-2_7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,12]],"date-time":"2023-03-12T00:15:02Z","timestamp":1678580102000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2740-2_7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,15]]},"ISBN":["9781071627396","9781071627402"],"references-count":20,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2740-2_7","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,15]]},"assertion":[{"value":"15 November 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}