{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T17:38:48Z","timestamp":1743097128746,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":16,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071627518"},{"type":"electronic","value":"9781071627525"}],"license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-2752-5_3","type":"book-chapter","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T18:59:07Z","timestamp":1669229947000},"page":"13-23","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proliferation Analysis of Cerebellar Granule Neuron Progenitors for Microcephaly Research, Using Immunofluorescent Staining and Flow Cytometry"],"prefix":"10.1007","author":[{"given":"Jennifer Karin","family":"Ocasio","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"key":"3_CR1","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1159\/000448639","volume":"7","author":"E Parrini","year":"2016","unstructured":"Parrini E, Conti V, Dobyns WB et al (2016) Genetic basis of brain malformations. Mol Syndromol 7:220\u2013233","journal-title":"Mol Syndromol"},{"key":"3_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fcell.2019.00029","volume":"7","author":"A Kalogeropoulou","year":"2019","unstructured":"Kalogeropoulou A, Lygerou Z, Taraviras S (2019) Cortical development and brain malformations: insights from the differential regulation of early events of DNA replication. Front Cell Dev Biol 7:1\u20139","journal-title":"Front Cell Dev Biol"},{"key":"3_CR3","doi-asserted-by":"publisher","first-page":"e7","DOI":"10.1017\/S0016672318000046","volume":"100","author":"M Naveed","year":"2018","unstructured":"Naveed M, Kazmi SK, Amin M et al (2018) Comprehensive review on the molecular genetics of autosomal recessive primary microcephaly (MCPH). Genet Res (Camb) 100:e7","journal-title":"Genet Res (Camb)"},{"key":"3_CR4","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1007\/s00441-014-1895-8","volume":"359","author":"LJA Hardwick","year":"2015","unstructured":"Hardwick LJA, Ali FR, Azzarelli R et al (2015) Cell cycle regulation of proliferation versus differentiation in the central nervous system. Cell Tissue Res 359:187\u2013200","journal-title":"Cell Tissue Res"},{"key":"3_CR5","doi-asserted-by":"publisher","first-page":"6533","DOI":"10.1523\/JNEUROSCI.0778-05.2005","volume":"25","author":"F Calegari","year":"2005","unstructured":"Calegari F, Haubensak W, Haffner C et al (2005) Selective lengthening of the cell cycle in the neurogenic subpopulation of neural progenitor cells during mouse brain development. J Neurosci 25:6533\u20136538","journal-title":"J Neurosci"},{"key":"3_CR6","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1002\/cne.23801","volume":"524","author":"M Turrero Garc\u00eda","year":"2016","unstructured":"Turrero Garc\u00eda M, Chang Y, Arai Y et al (2016) S-phase duration is the main target of cell cycle regulation in neural progenitors of developing ferret neocortex. J Comp Neurol 524:456\u2013470","journal-title":"J Comp Neurol"},{"key":"3_CR7","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1038\/ncomms1155","volume":"2","author":"Y Arai","year":"2011","unstructured":"Arai Y, Pulvers JN, Haffner C et al (2011) Neural stem and progenitor cells shorten S-phase on commitment to neuron production. Nat Commun 2:154","journal-title":"Nat Commun"},{"key":"3_CR8","doi-asserted-by":"publisher","first-page":"4038","DOI":"10.1242\/dev.139022","volume":"143","author":"PY Lang","year":"2016","unstructured":"Lang PY, Nanjangud GJ, Sokolsky-Papkov M et al (2016) ATR maintains chromosomal integrity during postnatal cerebellar neurogenesis and is required for medulloblastoma formation. Development. 143:4038\u20134052","journal-title":"Development."},{"key":"3_CR9","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.neuron.2015.12.007","volume":"89","author":"LJ Pilaz","year":"2016","unstructured":"Pilaz LJ, McMahon JJ, Miller EE et al (2016) Prolonged mitosis of neural progenitors alters cell fate in the developing brain. Neuron 89:83\u201399","journal-title":"Neuron"},{"key":"3_CR10","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1073\/pnas.44.5.476","volume":"44","author":"WL Hughes","year":"1958","unstructured":"Hughes WL, Bond VP, Brecher G et al (1958) Cellular proliferation in the mouse as revealed by autoradiography with Tritiated thymidine. Proc Natl Acad Sci 44:476\u2013483","journal-title":"Proc Natl Acad Sci"},{"key":"3_CR11","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1126\/science.7123245","volume":"218","author":"HG Graztner","year":"1982","unstructured":"Graztner HG (1982) Monoclonal antibody to 5-Bromo and 5-Iododeoxyuridine: a new reagent for detection of DNA replication. Science (80-) 218:474\u2013475","journal-title":"Science (80-)"},{"key":"3_CR12","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1002\/cyto.990120412","volume":"12","author":"PJM Bakker","year":"1991","unstructured":"Bakker PJM, Stap J, Tukker CJ et al (1991) An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo- and chlorodeoxyuridine incorporated into DNA. Cytometry 12:366\u2013372","journal-title":"Cytometry"},{"key":"3_CR13","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.brainres.2009.12.092","volume":"1319","author":"C Zeng","year":"2010","unstructured":"Zeng C, Pan F, Jones LA et al (2010) Evaluation of 5-ethynyl-2\u2032-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res 1319:21\u201332","journal-title":"Brain Res"},{"key":"3_CR14","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-1-4939-2596-4_7","volume-title":"DNA replication: methods and protocols","author":"N Talarek","year":"2015","unstructured":"Talarek N, Petit J, Gueydon E et al (2015) EdU incorporation for FACS and microscopy analysis of DNA replication in budding yeast. In: Vengrova S, Dalgaard J (eds) DNA replication: methods and protocols. Springer, New York, NY, pp 105\u2013112"},{"key":"3_CR15","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1007\/978-1-4419-6199-0_9","volume":"676","author":"Z Darzynkiewicz","year":"2010","unstructured":"Darzynkiewicz Z, Halicka HD, Zhao H (2010) Analysis of cellular DNA content by flow and laser scanning cytometry. Adv Exp Med Biol 676:137\u2013147","journal-title":"Adv Exp Med Biol"},{"key":"3_CR16","doi-asserted-by":"publisher","first-page":"5858","DOI":"10.1074\/jbc.273.10.5858","volume":"273","author":"EP Rogakou","year":"1998","unstructured":"Rogakou EP, Pilch DR, Orr AH et al (1998) DNA double-stranded breaks induce DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 273:5858\u20135868","journal-title":"J Biol Chem"}],"container-title":["Methods in Molecular Biology","Microcephaly"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2752-5_3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T21:14:52Z","timestamp":1678742092000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2752-5_3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,24]]},"ISBN":["9781071627518","9781071627525"],"references-count":16,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2752-5_3","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2022,11,24]]},"assertion":[{"value":"24 November 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}