{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T15:17:56Z","timestamp":1750778276636,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":9,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071628102"},{"type":"electronic","value":"9781071628119"}],"license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-2811-9_13","type":"book-chapter","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T19:04:33Z","timestamp":1670958273000},"page":"213-220","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-detection of Phosphorylated EGFR in A431 Cultured Cells"],"prefix":"10.1007","author":[{"given":"Jodi","family":"Hagen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley","family":"Oliver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander E.","family":"Kalyuzhny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"issue":"2","key":"13_CR1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.ijrobp.2003.11.041","volume":"59","author":"RS Herbst","year":"2004","unstructured":"Herbst RS (2004) Review of epidermal growth factor receptor biology. Int J Radiat Oncol Biol Phys 59(2):21\u201326","journal-title":"Int J Radiat Oncol Biol Phys"},{"issue":"4647","key":"13_CR2","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1126\/science.6200934","volume":"224","author":"GT Merlino","year":"1984","unstructured":"Merlino GT, Xu YH, Ishii S et al (1984) Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells. Science 224(4647):417\u2013419","journal-title":"Science"},{"issue":"1","key":"13_CR3","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","volume":"14","author":"F Wang","year":"2012","unstructured":"Wang F, Flanagan J, Su N et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14(1):22\u201329","journal-title":"J Mol Diagn"},{"key":"13_CR4","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/B978-012748920-9\/50005-4","volume-title":"Molecular methods for virus detection","author":"J Carr","year":"1995","unstructured":"Carr J, Wiedbrauk D, Farkas D (1995) In Situ Hybridization. In: Molecular methods for virus detection. Academic, San Diego, pp 75\u2013101"},{"issue":"7","key":"13_CR5","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1177\/42.7.8014480","volume":"42","author":"EJM Speel","year":"1994","unstructured":"Speel EJM, Herbergs J, Ramaekers FCS et al (1994) Combined immunocytochemistry and fluorescence in situ hybridization for simultaneous tricolor detection of cell cycle, genomic, and phenotypic parameters of tumor cells. J Histochem Cytochem 42(7):961\u2013966","journal-title":"J Histochem Cytochem"},{"key":"13_CR6","unstructured":"Nayak M, Dikshit A (2021) Webinar: introducing new RNA-protein co-detection assays. https:\/\/acdbio.com\/2021-mar-5-webinar-introducing-new-rna-protein-co-detection-assays"},{"key":"13_CR7","unstructured":"Advanced Cell Diagnostics. Documents and Downloads (2019) https:\/\/acdbio.com\/sites\/default\/files\/323100-TN%20Multiplex%20Fluorescent%20V2%20with%20IF_incOpal780.pdf"},{"key":"13_CR8","unstructured":"Advanced Cell Diagnostics. Documents and Downloads (2019) https:\/\/acdbio.com\/sites\/default\/files\/MK%2050%20010_Tech%20Note%20CulturedCells%20Fluorescent%20v2_05312019.pdf"},{"key":"13_CR9","unstructured":"Advanced Cell Diagnostics. Documents and Downloads (2019) https:\/\/acdbio.com\/sites\/default\/files\/USM-323100%20Multiplex%20Fluorescent%20v2%20User%20Manual_10282019_0.pdf"}],"container-title":["Methods in Molecular Biology","Signal Transduction Immunohistochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-2811-9_13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T19:07:17Z","timestamp":1670958437000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-2811-9_13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,14]]},"ISBN":["9781071628102","9781071628119"],"references-count":9,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-2811-9_13","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2022,12,14]]},"assertion":[{"value":"14 December 2022","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}