{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:52:07Z","timestamp":1788346327053,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":17,"publisher":"Springer US","isbn-type":[{"value":"9781071631386","type":"print"},{"value":"9781071631393","type":"electronic"}],"license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-3139-3_3","type":"book-chapter","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T13:02:55Z","timestamp":1685451775000},"page":"21-35","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes"],"prefix":"10.1007","author":[{"given":"Victoria D.","family":"Diaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian P.","family":"Hermann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,31]]},"reference":[{"key":"3_CR1","doi-asserted-by":"publisher","unstructured":"Di Carlo D, Tse HTK, Gossett DR (2012) Introduction: Why analyze single cells? Methods Mol Biol 853:1\u201310. https:\/\/doi.org\/10.1007\/978-1-61779-567-1_1","DOI":"10.1007\/978-1-61779-567-1_1"},{"key":"3_CR2","doi-asserted-by":"publisher","unstructured":"Velte EK, Niedenberger BA, Serra ND, Singh A, Roa-De La Cruz L, Hermann BP et al (2019) Differential RA responsiveness directs formation of functionally distinct spermatogonial populations at the initiation of spermatogenesis in the mouse. 146(12):dev.173088 [pii]. https:\/\/doi.org\/10.1242\/dev.173088","DOI":"10.1242\/dev.173088"},{"issue":"5","key":"3_CR3","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1038\/nmeth.1315","volume":"6","author":"F Tang","year":"2009","unstructured":"Tang F, Barbacioru C, Wang Y, Nordman E, Lee C, Xu N et al (2009) mRNA-Seq whole-transcriptome analysis of a single cell. Nat Methods 6(5):377\u2013382. https:\/\/doi.org\/10.1038\/nmeth.1315","journal-title":"Nat Methods"},{"issue":"7341","key":"3_CR4","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1038\/nature09807","volume":"472","author":"N Navin","year":"2011","unstructured":"Navin N, Kendall J, Troge J, Andrews P, Rodgers L, McIndoo J et al (2011) Tumour evolution inferred by single-cell sequencing. Nature 472(7341):90\u201394. https:\/\/doi.org\/10.1038\/nature09807","journal-title":"Nature"},{"issue":"7","key":"3_CR5","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1101\/gr.110882.110","volume":"21","author":"S Islam","year":"2011","unstructured":"Islam S, Kjallquist U, Moliner A, Zajac P, Fan JB, Lonnerberg P et al (2011) Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq. Genome Res 21(7):1160\u20131167. https:\/\/doi.org\/10.1101\/gr.110882.110","journal-title":"Genome Res"},{"issue":"11","key":"3_CR6","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1038\/nmeth.2639","volume":"10","author":"S Picelli","year":"2013","unstructured":"Picelli S, Bjorklund AK, Faridani OR, Sagasser S, Winberg G, Sandberg R (2013) Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat Methods 10(11):1096\u20131098. https:\/\/doi.org\/10.1038\/nmeth.2639","journal-title":"Nat Methods"},{"issue":"3","key":"3_CR7","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1016\/j.celrep.2012.08.003","volume":"2","author":"T Hashimshony","year":"2012","unstructured":"Hashimshony T, Wagner F, Sher N, Yanai I (2012) CEL-Seq: single-cell RNA-Seq by multiplexed linear amplification. Cell Rep 2(3):666\u2013673. https:\/\/doi.org\/10.1016\/j.celrep.2012.08.003","journal-title":"Cell Rep"},{"issue":"3","key":"3_CR8","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1093\/biolre\/ioz088","volume":"101","author":"S Suzuki","year":"2019","unstructured":"Suzuki S, Diaz VD, Hermann BP (2019) What has single-cell RNA-seq taught us about mammalian spermatogenesis? Biol Reprod 101(3):617\u2013634. https:\/\/doi.org\/10.1093\/biolre\/ioz088","journal-title":"Biol Reprod"},{"issue":"6","key":"3_CR9","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1016\/j.celrep.2018.10.026","volume":"25","author":"BP Hermann","year":"2018","unstructured":"Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC et al (2018) The mammalian spermatogenesis single-cell transcriptome, from spermatogonial stem cells to spermatids. Cell Rep 25(6):1650\u20131667. e8. https:\/\/doi.org\/10.1016\/j.celrep.2018.10.026","journal-title":"Cell Rep"},{"issue":"2","key":"3_CR10","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1095\/biolreprod.114.125757","volume":"92","author":"BP Hermann","year":"2015","unstructured":"Hermann BP, Mutoji KN, Velte EK, Ko D, Oatley JM, Geyer CB et al (2015) Transcriptional and translational heterogeneity among neonatal mouse spermatogonia. Biol Reprod 92(2):54. https:\/\/doi.org\/10.1095\/biolreprod.114.125757","journal-title":"Biol Reprod"},{"issue":"2","key":"3_CR11","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1073\/pnas.63.2.378","volume":"63","author":"JG Gall","year":"1969","unstructured":"Gall JG, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A 63(2):378\u2013383. https:\/\/doi.org\/10.1073\/pnas.63.2.378","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"3_CR12","doi-asserted-by":"publisher","first-page":"600","DOI":"10.1073\/pnas.64.2.600","volume":"64","author":"ML Pardue","year":"1969","unstructured":"Pardue ML, Gall JG (1969) Molecular hybridization of radioactive DNA to the DNA of cytological preparations. Proc Natl Acad Sci U S A 64(2):600\u2013604. https:\/\/doi.org\/10.1073\/pnas.64.2.600","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"28","key":"3_CR13","doi-asserted-by":"publisher","first-page":"E5635","DOI":"10.1073\/pnas.1701069114","volume":"114","author":"SJ Newkirk","year":"2017","unstructured":"Newkirk SJ, Lee S, Grandi FC, Gaysinskaya V, Rosser JM, Vanden Berg N et al (2017) Intact piRNA pathway prevents L1 mobilization in male meiosis. Proc Natl Acad Sci U S A 114(28):E5635\u2013E5E44. https:\/\/doi.org\/10.1073\/pnas.1701069114","journal-title":"Proc Natl Acad Sci U S A"},{"key":"3_CR14","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s12575-018-0071-z","volume":"20","author":"N Takei","year":"2018","unstructured":"Takei N, Nakamura T, Kawamura S, Takada Y, Satoh Y, Kimura AP et al (2018) High-sensitivity and high-resolution in situ hybridization of coding and long non-coding RNAs in vertebrate ovaries and testes. Biol Proced Online 20:6. https:\/\/doi.org\/10.1186\/s12575-018-0071-z","journal-title":"Biol Proced Online"},{"issue":"1","key":"3_CR15","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1101\/gr.264333.120","volume":"31","author":"K Li","year":"2021","unstructured":"Li K, Xu J, Luo Y, Zou D, Han R, Zhong S et al (2021) Panoramic transcriptome analysis and functional screening of long noncoding RNAs in mouse spermatogenesis. Genome Res 31(1):13\u201326. https:\/\/doi.org\/10.1101\/gr.264333.120","journal-title":"Genome Res"},{"issue":"1","key":"3_CR16","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jmoldx.2011.08.002","volume":"14","author":"F Wang","year":"2012","unstructured":"Wang F, Flanagan J, Su N, Wang LC, Bui S, Nielson A et al (2012) RNAscope: a novel in situ RNA analysis platform for formalin-fixed, paraffin-embedded tissues. J Mol Diagn 14(1):22\u201329. https:\/\/doi.org\/10.1016\/j.jmoldx.2011.08.002","journal-title":"J Mol Diagn"},{"issue":"5","key":"3_CR17","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/j.devcel.2018.07.025","volume":"46","author":"CD Green","year":"2018","unstructured":"Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S et al (2018) A comprehensive roadmap of murine spermatogenesis defined by single-cell RNA-Seq. Dev Cell 46(5):651\u2013667. e10. https:\/\/doi.org\/10.1016\/j.devcel.2018.07.025","journal-title":"Dev Cell"}],"container-title":["Methods in Molecular Biology","Spermatogonial Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3139-3_3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T13:03:10Z","timestamp":1685451790000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3139-3_3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"ISBN":["9781071631386","9781071631393"],"references-count":17,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3139-3_3","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]},"assertion":[{"value":"31 May 2023","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}