{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:21:36Z","timestamp":1783099296004,"version":"3.54.6"},"publisher-location":"New York, NY","reference-count":43,"publisher":"Springer US","isbn-type":[{"value":"9781071631904","type":"print"},{"value":"9781071631911","type":"electronic"}],"license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-3191-1_9","type":"book-chapter","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T14:02:40Z","timestamp":1683813760000},"page":"115-136","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vivo Cross-Linking and Co-Immunoprecipitation Procedure to Analyze Nuclear tRNA Export Complexes in Yeast Cells"],"prefix":"10.1007","author":[{"given":"Kunal","family":"Chatterjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita K.","family":"Hopper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,12]]},"reference":[{"key":"9_CR1","doi-asserted-by":"publisher","first-page":"21042","DOI":"10.1073\/pnas.1316579110","volume":"110","author":"EB Kramer","year":"2013","unstructured":"Kramer EB, Hopper AK (2013) Retrograde transfer RNA nuclear import provides a new level of tRNA quality control in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 110:21042\u201321047","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR2","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/j.bbagrm.2017.11.007","volume":"1861","author":"K Chatterjee","year":"2018","unstructured":"Chatterjee K, Nostramo RT, Wan Y, Hopper AK (2018) tRNA dynamics between the nucleus, cytoplasm and mitochondrial surface: location, location, location. Biochim Biophys Acta Gene Regul Mech 1861:373\u2013386","journal-title":"Biochim Biophys Acta Gene Regul Mech"},{"key":"9_CR3","doi-asserted-by":"publisher","first-page":"8845","DOI":"10.1073\/pnas.0700765104","volume":"104","author":"HH Shaheen","year":"2007","unstructured":"Shaheen HH, Horetsky RL, Kimball SR, Murthi A, Jefferson LS, Hopper AK (2007) Retrograde nuclear accumulation of cytoplasmic tRNA in rat hepatoma cells in response to amino acid deprivation. Proc Natl Acad Sci U S A 104:8845\u20138850","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR4","doi-asserted-by":"publisher","first-page":"2002","DOI":"10.1093\/nar\/gky1208","volume":"47","author":"R Dhakal","year":"2019","unstructured":"Dhakal R, Tong C, Anderson S, Kashina AS, Cooperman B, Bau HH (2019) Dynamics of intracellular stress-induced tRNA trafficking. Nucleic Acids Res 47:2002\u20132010","journal-title":"Nucleic Acids Res"},{"issue":"3416\u20133428","key":"9_CR5","volume":"26","author":"H Schwenzer","year":"2019","unstructured":"Schwenzer H, Juhling F, Chu A, Pallett LJ, Baumert TF, Maini M, Fassati A (2019) Oxidative stress triggers selective tRNA retrograde transport in human cells during the integrated stress response. Cell Rep 26(3416\u20133428):e3415","journal-title":"Cell Rep"},{"key":"9_CR6","doi-asserted-by":"publisher","first-page":"158","DOI":"10.3389\/fgene.2014.00158","volume":"5","author":"JA Abbott","year":"2014","unstructured":"Abbott JA, Francklyn CS, Robey-Bond SM (2014) Transfer RNA and human disease. Front Genet 5:158","journal-title":"Front Genet"},{"key":"9_CR7","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/S0092-8674(80)80050-X","volume":"19","author":"AK Hopper","year":"1980","unstructured":"Hopper AK, Schultz LD, Shapiro RA (1980) Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs. Cell 19:741\u2013751","journal-title":"Cell"},{"key":"9_CR8","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/S0960-9822(98)70130-7","volume":"8","author":"GJ Arts","year":"1998","unstructured":"Arts GJ, Fornerod M, Mattaj IW (1998) Identification of a nuclear export receptor for tRNA. Curr Biol 8:305\u2013314","journal-title":"Curr Biol"},{"key":"9_CR9","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/S1097-2765(00)80036-2","volume":"1","author":"U Kutay","year":"1998","unstructured":"Kutay U, Lipowsky G, Izaurralde E, Bischoff FR, Schwarzmaier P, Hartmann E, Gorlich D (1998) Identification of a tRNA-specific nuclear export receptor. Mol Cell 1:359\u2013369","journal-title":"Mol Cell"},{"key":"9_CR10","doi-asserted-by":"publisher","first-page":"480","DOI":"10.1091\/mbc.e11-08-0732","volume":"23","author":"V Cherkasova","year":"2012","unstructured":"Cherkasova V, Maury LL, Bacikova D, Pridham K, Bahler J, Maraia RJ (2012) Altered nuclear tRNA metabolism in La-deleted Schizosaccharomyces pombe is accompanied by a nutritional stress response involving Atf1p and Pcr1p that is suppressible by Xpo-t\/Los1p. Mol Biol Cell 23:480\u2013491","journal-title":"Mol Biol Cell"},{"key":"9_CR11","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1104\/pp.103.023309","volume":"132","author":"CA Hunter","year":"2003","unstructured":"Hunter CA, Aukerman MJ, Sun H, Fokina M, Poethig RS (2003) PAUSED encodes the Arabidopsis exportin-t ortholog. Plant Physiol 132:2135\u20132143","journal-title":"Plant Physiol"},{"key":"9_CR12","first-page":"1208","volume":"7","author":"DJ Hurt","year":"1987","unstructured":"Hurt DJ, Wang SS, Lin YH, Hopper AK (1987) Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing. Mol Cell Biol 7:1208\u20131216","journal-title":"Mol Cell Biol"},{"key":"9_CR13","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1038\/nature08394","volume":"461","author":"AG Cook","year":"2009","unstructured":"Cook AG, Fukuhara N, Jinek M, Conti E (2009) Structures of the tRNA export factor in the nuclear and cytosolic states. Nature 461:60\u201365","journal-title":"Nature"},{"key":"9_CR14","first-page":"82","volume":"3","author":"T Cavazza","year":"2015","unstructured":"Cavazza T, Vernos I (2015) The RanGTP pathway: from nucleo-cytoplasmic transport to spindle assembly and beyond. Front Cell Dev Biol 3:82","journal-title":"Front Cell Dev Biol"},{"key":"9_CR15","doi-asserted-by":"publisher","first-page":"a000562","DOI":"10.1101\/cshperspect.a000562","volume":"2","author":"SR Wente","year":"2010","unstructured":"Wente SR, Rout MP (2010) The nuclear pore complex and nuclear transport. Cold Spring Harb Perspect Biol 2:a000562","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"9_CR16","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1126\/science.1142204","volume":"318","author":"LJ Terry","year":"2007","unstructured":"Terry LJ, Shows EB, Wente SR (2007) Crossing the nuclear envelope: hierarchical regulation of nucleocytoplasmic transport. Science 318:1412\u20131416","journal-title":"Science"},{"key":"9_CR17","doi-asserted-by":"publisher","first-page":"7430","DOI":"10.1093\/emboj\/17.24.7430","volume":"17","author":"GJ Arts","year":"1998","unstructured":"Arts GJ, Kuersten S, Romby P, Ehresmann B, Mattaj IW (1998) The role of exportin-t in selective nuclear export of mature tRNAs. EMBO J 17:7430\u20137441","journal-title":"EMBO J"},{"key":"9_CR18","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.1126\/science.282.5396.2082","volume":"282","author":"E Lund","year":"1998","unstructured":"Lund E, Dahlberg JE (1998) Proofreading and aminoacylation of tRNAs before export from the nucleus. Science 282:2082\u20132085","journal-title":"Science"},{"key":"9_CR19","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1017\/S1355838299982134","volume":"5","author":"G Lipowsky","year":"1999","unstructured":"Lipowsky G, Bischoff FR, Izaurralde E, Kutay U, Schafer S, Gross HJ, Beier H, Gorlich D (1999) Coordination of tRNA nuclear export with processing of tRNA. RNA 5:539\u2013549","journal-title":"RNA"},{"key":"9_CR20","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1101\/gad.258293.115","volume":"29","author":"HY Huang","year":"2015","unstructured":"Huang HY, Hopper AK (2015) In vivo biochemical analyses reveal distinct roles of beta-importins and eEF1A in tRNA subcellular traffic. Genes Dev 29:772\u2013783","journal-title":"Genes Dev"},{"key":"9_CR21","doi-asserted-by":"publisher","first-page":"6374","DOI":"10.1128\/MCB.18.11.6374","volume":"18","author":"K Hellmuth","year":"1998","unstructured":"Hellmuth K, Lau DM, Bischoff FR, Kunzler M, Hurt E, Simos G (1998) Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA. Mol Cell Biol 18:6374\u20136386","journal-title":"Mol Cell Biol"},{"key":"9_CR22","doi-asserted-by":"publisher","first-page":"1913","DOI":"10.1104\/pp.103.023291","volume":"132","author":"J Li","year":"2003","unstructured":"Li J, Chen X (2003) PAUSED, a putative exportin-t, acts pleiotropically in Arabidopsis development but is dispensable for viability. Plant Physiol 132:1913\u20131924","journal-title":"Plant Physiol"},{"key":"9_CR23","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1126\/science.aac7557","volume":"350","author":"VA Blomen","year":"2015","unstructured":"Blomen VA, Majek P, Jae LT, Bigenzahn JW, Nieuwenhuis J, Staring J, Sacco R, van Diemen FR, Olk N, Stukalov A et al (2015) Gene essentiality and synthetic lethality in haploid human cells. Science 350:1092\u20131096","journal-title":"Science"},{"key":"9_CR24","doi-asserted-by":"publisher","first-page":"1515","DOI":"10.1016\/j.cell.2015.11.015","volume":"163","author":"T Hart","year":"2015","unstructured":"Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S et al (2015) High-resolution CRISPR screens reveal fitness genes and genotype-specific cancer liabilities. Cell 163:1515\u20131526","journal-title":"Cell"},{"key":"9_CR25","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1126\/science.aac7041","volume":"350","author":"T Wang","year":"2015","unstructured":"Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM (2015) Identification and characterization of essential genes in the human genome. Science 350:1096\u20131101","journal-title":"Science"},{"key":"9_CR26","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1093\/genetics\/156.4.1889","volume":"156","author":"M Lippai","year":"2000","unstructured":"Lippai M, Tirian L, Boros I, Mihaly J, Erdelyi M, Belecz I, Mathe E, Posfai J, Nagy A, Udvardy A et al (2000) The Ketel gene encodes a drosophila homologue of importin-beta. Genetics 156:1889\u20131900","journal-title":"Genetics"},{"key":"9_CR27","doi-asserted-by":"publisher","first-page":"8620","DOI":"10.1093\/nar\/gkz671","volume":"47","author":"E Hegedusova","year":"2019","unstructured":"Hegedusova E, Kulkarni S, Burgman B, Alfonzo JD, Paris Z (2019) The general mRNA exporters Mex67 and Mtr2 play distinct roles in nuclear export of tRNAs in Trypanosoma brucei. Nucleic Acids Res 47:8620\u20138631","journal-title":"Nucleic Acids Res"},{"key":"9_CR28","doi-asserted-by":"publisher","first-page":"2633","DOI":"10.1101\/gad.269803.115","volume":"29","author":"J Wu","year":"2015","unstructured":"Wu J, Bao A, Chatterjee K, Wan Y, Hopper AK (2015) Genome-wide screen uncovers novel pathways for tRNA processing and nuclear-cytoplasmic dynamics. Genes Dev 29:2633\u20132644","journal-title":"Genes Dev"},{"key":"9_CR29","doi-asserted-by":"publisher","first-page":"2186","DOI":"10.1101\/gad.305904.117","volume":"31","author":"K Chatterjee","year":"2017","unstructured":"Chatterjee K, Majumder S, Wan Y, Shah V, Wu J, Huang HY, Hopper AK (2017) Sharing the load: Mex67-Mtr2 cofunctions with Los1 in primary tRNA nuclear export. Genes Dev 31:2186\u20132198","journal-title":"Genes Dev"},{"key":"9_CR30","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1534\/genetics.112.147470","volume":"194","author":"AK Hopper","year":"2013","unstructured":"Hopper AK (2013) Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae. Genetics 194:43\u201367","journal-title":"Genetics"},{"key":"9_CR31","doi-asserted-by":"publisher","first-page":"3266","DOI":"10.1091\/mbc.e02-11-0757","volume":"14","author":"T Yoshihisa","year":"2003","unstructured":"Yoshihisa T, Yunoki-Esaki K, Ohshima C, Tanaka N, Endo T (2003) Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria. Mol Biol Cell 14:3266\u20133279","journal-title":"Mol Biol Cell"},{"key":"9_CR32","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1111\/j.1365-2443.2007.01056.x","volume":"12","author":"T Yoshihisa","year":"2007","unstructured":"Yoshihisa T, Ohshima C, Yunoki-Esaki K, Endo T (2007) Cytoplasmic splicing of tRNA in Saccharomyces cerevisiae. Genes Cells 12:285\u2013297","journal-title":"Genes Cells"},{"key":"9_CR33","doi-asserted-by":"publisher","first-page":"1309","DOI":"10.1101\/gad.316257.118","volume":"32","author":"Y Wan","year":"2018","unstructured":"Wan Y, Hopper AK (2018) From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing. Genes Dev 32:1309\u20131314","journal-title":"Genes Dev"},{"key":"9_CR34","doi-asserted-by":"publisher","first-page":"2840","DOI":"10.1091\/mbc.e14-04-0948","volume":"25","author":"HY Huang","year":"2014","unstructured":"Huang HY, Hopper AK (2014) Separate responses of karyopherins to glucose and amino acid availability regulate nucleocytoplasmic transport. Mol Biol Cell 25:2840\u20132852","journal-title":"Mol Biol Cell"},{"key":"9_CR35","doi-asserted-by":"publisher","first-page":"951","DOI":"10.1038\/nmeth1101","volume":"4","author":"M Oeffinger","year":"2007","unstructured":"Oeffinger M, Wei KE, Rogers R, DeGrasse JA, Chait BT, Aitchison JD, Rout MP (2007) Comprehensive analysis of diverse ribonucleoprotein complexes. Nat Methods 4:951\u2013956","journal-title":"Nat Methods"},{"key":"9_CR36","doi-asserted-by":"crossref","unstructured":"Chatterjee K, Marshall WA, Hopper AK (2022) Three tRNA nuclear exporters in S. cerevisiae: parallel pathways, preferences, and precision. Nucleic Acids Res 50(17):10140\u201310152","DOI":"10.1093\/nar\/gkac754"},{"key":"9_CR37","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/S0092-8674(00)81666-9","volume":"99","author":"S Nakielny","year":"1999","unstructured":"Nakielny S, Dreyfuss G (1999) Transport of proteins and RNAs in and out of the nucleus. Cell 99:677\u2013690","journal-title":"Cell"},{"key":"9_CR38","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1083\/jcb.125.4.705","volume":"125","author":"S Kornbluth","year":"1994","unstructured":"Kornbluth S, Dasso M, Newport J (1994) Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. J Cell Biol 125:705\u2013719","journal-title":"J Cell Biol"},{"key":"9_CR39","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1038\/nbt.1832","volume":"29","author":"Z Li","year":"2011","unstructured":"Li Z, Vizeacoumar FJ, Bahr S, Li J, Warringer J, Vizeacoumar FS, Min R, Vandersluis B, Bellay J, Devit M et al (2011) Systematic exploration of essential yeast gene function with temperature-sensitive mutants. Nat Biotechnol 29:361\u2013367","journal-title":"Nat Biotechnol"},{"key":"9_CR40","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1016\/j.celrep.2014.08.021","volume":"8","author":"H Wu","year":"2014","unstructured":"Wu H, Becker D, Krebber H (2014) Telomerase RNA TLC1 shuttling to the cytoplasm requires mRNA export factors and is important for telomere maintenance. Cell Rep 8:1630\u20131638","journal-title":"Cell Rep"},{"key":"9_CR41","doi-asserted-by":"publisher","first-page":"11290","DOI":"10.1073\/pnas.0503836102","volume":"102","author":"HH Shaheen","year":"2005","unstructured":"Shaheen HH, Hopper AK (2005) Retrograde movement of tRNAs from the cytoplasm to the nucleus in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102:11290\u201311295","journal-title":"Proc Natl Acad Sci U S A"},{"key":"9_CR42","doi-asserted-by":"publisher","first-page":"e04659","DOI":"10.7554\/eLife.04659","volume":"4","author":"A Takano","year":"2015","unstructured":"Takano A, Kajita T, Mochizuki M, Endo T, Yoshihisa T (2015) Cytosolic Hsp70 and co-chaperones constitute a novel system for tRNA import into the nucleus. elife 4:e04659","journal-title":"elife"},{"key":"9_CR43","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S0076-6879(07)25002-5","volume":"425","author":"Y Motorin","year":"2007","unstructured":"Motorin Y, Muller S, Behm-Ansmant I, Branlant C (2007) Identification of modified residues in RNAs by reverse transcription-based methods. Methods Enzymol 425:21\u201353","journal-title":"Methods Enzymol"}],"container-title":["Methods in Molecular Biology","RNA-Protein Complexes and Interactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3191-1_9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T14:06:09Z","timestamp":1683813969000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3191-1_9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"ISBN":["9781071631904","9781071631911"],"references-count":43,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3191-1_9","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]},"assertion":[{"value":"12 May 2023","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}