{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T01:04:32Z","timestamp":1782954272629,"version":"3.54.5"},"publisher-location":"New York, NY","reference-count":33,"publisher":"Springer US","isbn-type":[{"value":"9781071632062","type":"print"},{"value":"9781071632079","type":"electronic"}],"license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023]]},"DOI":"10.1007\/978-1-0716-3207-9_1","type":"book-chapter","created":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T08:02:15Z","timestamp":1685347335000},"page":"1-32","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update"],"prefix":"10.1007","author":[{"given":"Steffen K.","family":"Meurer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Weiskirchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen G.","family":"Tag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,5,30]]},"reference":[{"issue":"3","key":"1_CR1","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1679\/aohc1950.43.265","volume":"43","author":"H Fujita","year":"1980","unstructured":"Fujita H, Tamaru T, Miyagawa J (1980) Fine structural characteristics of the hepatic sinusoidal walls of the goldfish (Carassius auratus). Arch Histol Jpn 43(3):265\u2013273. https:\/\/doi.org\/10.1679\/aohc1950.43.265","journal-title":"Arch Histol Jpn"},{"issue":"1","key":"1_CR2","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1515\/hsz-2019-0283","volume":"401","author":"D H\u00e4ussinger","year":"2019","unstructured":"H\u00e4ussinger D, Kordes C (2019) Space of Disse: a stem cell niche in the liver. Biol Chem 401(1):81\u201395. https:\/\/doi.org\/10.1515\/hsz-2019-0283","journal-title":"Biol Chem"},{"key":"1_CR3","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1586\/egh.11.92","volume":"6","author":"F Tacke","year":"2012","unstructured":"Tacke F, Weiskirchen R (2012) Update on hepatic stellate cells: pathogenic role in liver fibrosis and novel isolation techniques. Expert Rev Gastroenterol Hepatol 6:67\u201380. https:\/\/doi.org\/10.1586\/egh.11.92","journal-title":"Expert Rev Gastroenterol Hepatol"},{"key":"1_CR4","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1016\/0014-4827(82)90283-X","volume":"139","author":"DL Knook","year":"1982","unstructured":"Knook DL, Seffelaar AM, de Leeuw AM (1982) Fat-storing cells of the rat liver. Their isolation and purification. Exp Cell Res 139:468\u2013471. https:\/\/doi.org\/10.1016\/0014-4827(82)90283-X","journal-title":"Exp Cell Res"},{"key":"1_CR5","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/0003-2697(87)90673-7","volume":"161","author":"SL Friedman","year":"1987","unstructured":"Friedman SL, Roll FJ (1987) Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan. Anal Biochem 161:207\u2013218. https:\/\/doi.org\/10.1016\/0003-2697(87)90673-7","journal-title":"Anal Biochem"},{"key":"1_CR6","doi-asserted-by":"publisher","first-page":"1786","DOI":"10.1172\/JCI114363","volume":"84","author":"M Pinzani","year":"1989","unstructured":"Pinzani M, Gesualdo L, Sabbah GM, Abboud HE (1989) Effects of platelet-derived growth factor and other polypeptide mitogens on DNA synthesis and growth of cultured rat liver fat-storing cells. J Clin Invest 84:1786\u20131793. https:\/\/doi.org\/10.1172\/JCI114363","journal-title":"J Clin Invest"},{"key":"1_CR7","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1002\/hep.1840040307","volume":"4","author":"AM de Leeuw","year":"1984","unstructured":"de Leeuw AM, McCarthy SP, Geerts A, Knook DL (1984) Purified rat liver fat-storing cells in culture divide and contain collagen. Hepatology 4:392\u2013403. https:\/\/doi.org\/10.1002\/hep.1840040307","journal-title":"Hepatology"},{"key":"1_CR8","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1002\/hep.1840070411","volume":"7","author":"S Sch\u00e4fer","year":"1987","unstructured":"Sch\u00e4fer S, Zerbe O, Gressner AM (1987) The synthesis of proteoglycans in fat-storing cells of rat liver. Hepatology 7:680\u2013687. https:\/\/doi.org\/10.1002\/hep.1840070411","journal-title":"Hepatology"},{"key":"1_CR9","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1385\/1-59259-940-0:099","volume":"117","author":"R Weiskirchen","year":"2005","unstructured":"Weiskirchen R, Gressner AM (2005) Isolation and culture of hepatic stellate cells. Methods Mol Med. 117:99\u2013113. https:\/\/doi.org\/10.1385\/1-59259-940-0:099","journal-title":"Methods Mol Med."},{"key":"1_CR10","doi-asserted-by":"publisher","unstructured":"Maschmeyer P, Flach M, Winau F (2011) Seven steps to stellate cells. J Vis Exp 10(51). https:\/\/doi.org\/10.3791\/2710","DOI":"10.3791\/2710"},{"key":"1_CR11","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1002\/hep.510300327","volume":"30","author":"AM Elsharkawy","year":"1999","unstructured":"Elsharkawy AM, Wright MC, Hay RT, Arthur MJ, Hughes T, Bahr MJ, Degitz K, Mann DA (1999) Persistent activation of nuclear factor-kappaB in cultured rat hepatic stellate cells involves the induction of potentially novel Rel-like factors and prolonged changes in the expression of IkappaB family proteins. Hepatology 30:761\u2013769. https:\/\/doi.org\/10.1002\/hep.510300327","journal-title":"Hepatology"},{"key":"1_CR12","doi-asserted-by":"publisher","first-page":"1347","DOI":"10.1100\/tsw.2002.283","volume":"2","author":"JM Graham","year":"2002","unstructured":"Graham JM (2002) Fractionation of hepatic nonparenchymal cells. ScientificWorldJournal 2:1347\u20131350. https:\/\/doi.org\/10.1100\/tsw.2002.283","journal-title":"ScientificWorldJournal"},{"issue":"21","key":"1_CR13","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.3422","volume":"9","author":"RV Modak","year":"2019","unstructured":"Modak RV, Zaiss DM (2019) Isolation and culture of murine hepatic stellate cells. Bio Protoc. 9(21):e3422. https:\/\/doi.org\/10.21769\/BioProtoc.3422","journal-title":"Bio Protoc."},{"key":"1_CR14","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/0076-6879(90)90009-P","volume":"190","author":"R Blomhoff","year":"1990","unstructured":"Blomhoff R, Berg T (1990) Isolation and cultivation of rat liver stellate cells. Methods Enzymol 190:58\u201371. https:\/\/doi.org\/10.1016\/0076-6879(90)90009-P","journal-title":"Methods Enzymol"},{"issue":"9","key":"1_CR15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024993","volume":"6","author":"DN D\u2019Ambrosio","year":"2011","unstructured":"D\u2019Ambrosio DN, Walewski JL, Clugston RD, Berk PD, Rippe RA, Blaner WS (2011) Distinct populations of hepatic stellate cells in the mouse liver have different capacities for retinoid and lipid storage. PLoS One 6(9):e24993. https:\/\/doi.org\/10.1371\/journal.pone.0024993","journal-title":"PLoS One"},{"key":"1_CR16","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1002\/hep.510270238","volume":"27","author":"A Geerts","year":"1998","unstructured":"Geerts A, Niki T, Hellemans K, De Craemer D, Van Den Berg K, Lazou JM, Stange G, Van De Winkel M, De Bleser P (1998) Purification of rat hepatic stellate cells by side scatter-activated cell sorting. Hepatology 27:590\u2013598. https:\/\/doi.org\/10.1002\/hep.510270238","journal-title":"Hepatology"},{"key":"1_CR17","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/417023","volume":"2015","author":"M Bartneck","year":"2015","unstructured":"Bartneck M, Warzecha KT, Tag CG, Sauer-Lehnen S, Heymann F, Trautwein C, Weiskirchen R, Tacke F (2015) Isolation and time lapse microscopy of highly pure hepatic stellate cells. Anal Cell Pathol (Amst) 2015:417023. https:\/\/doi.org\/10.1155\/2015\/417023","journal-title":"Anal Cell Pathol (Amst)"},{"key":"1_CR18","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1038\/nprot.2015.017","volume":"10","author":"I Mederacke","year":"2015","unstructured":"Mederacke I, Dapito DH, Aff\u00f2 S, Uchinami H, Schwabe RF (2015) High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers. Nat Protoc 10:305\u2013315. https:\/\/doi.org\/10.1038\/nprot.2015.017","journal-title":"Nat Protoc"},{"key":"1_CR19","doi-asserted-by":"publisher","first-page":"1730","DOI":"10.1002\/hep.24281","volume":"53","author":"YH Paik","year":"2011","unstructured":"Paik YH, Iwaisako K, Seki E, Inokuchi S, Schnabl B, Osterreicher CH, Kisseleva T, Brenner DA (2011) The nicotinamide adenine dinucleotide phosphate oxidase (NOX) homologues NOX1 and NOX2\/gp91(phox) mediate hepatic fibrosis in mice. Hepatology 53:1730\u20131741. https:\/\/doi.org\/10.1002\/hep.24281","journal-title":"Hepatology"},{"key":"1_CR20","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_11","volume-title":"Fibrosis. methods in molecular biology","author":"S Weiskirchen","year":"2017","unstructured":"Weiskirchen S, Tag CG, Sauer-Lehnen S, Tacke F, Weiskirchen R (2017) Isolation and culture of primary murine hepatic stellate cells. In: Ritti\u00e9 L (ed) Fibrosis. methods in molecular biology, vol 1627. Humana Press, New York. https:\/\/doi.org\/10.1007\/978-1-4939-7113-8_11"},{"key":"1_CR21","doi-asserted-by":"publisher","first-page":"980","DOI":"10.1053\/jhep.2000.18658","volume":"32","author":"YQ Wang","year":"2000","unstructured":"Wang YQ, Ikeda K, Ikebe T, Hirakawa K, Sowa M, Nakatani K, Kawada N, Kaneda K (2000) Inhibition of hepatic stellate cell proliferation and activation by the semisynthetic analogue of fumagillin TNP-470 in rats. Hepatology 32:980\u2013989. https:\/\/doi.org\/10.1053\/jhep.2000.18658","journal-title":"Hepatology"},{"key":"1_CR22","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.671640","volume":"12","author":"P Acharya","year":"2012","unstructured":"Acharya P, Chouhan K, Weiskirchen S, Weiskirchen R (2012) Cellular mechanisms of liver fibrosis. Front Pharmacol 12:671640. https:\/\/doi.org\/10.3389\/fphar.2021.671640","journal-title":"Front Pharmacol"},{"key":"1_CR23","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/j.cll.2007.05.013","volume":"27","author":"AD Donnenberg","year":"2007","unstructured":"Donnenberg AD, Donnenberg VS (2007) Rare-event analysis in flow cytometry. Clin Lab Med 27:627\u2013652. https:\/\/doi.org\/10.1016\/j.cll.2007.05.013","journal-title":"Clin Lab Med"},{"key":"1_CR24","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1016\/j.jhep.2005.07.008","volume":"44","author":"S Neyzen","year":"2006","unstructured":"Neyzen S, Van de Leur E, Borkham-Kamphorst E, Herrmann J, Hollweg G, Gressner AM, Weiskirchen R (2006) Cryopreservation of hepatic stellate cells. J Hepatol 44:910\u2013917. https:\/\/doi.org\/10.1016\/j.jhep.2005.07.008","journal-title":"J Hepatol"},{"key":"1_CR25","doi-asserted-by":"publisher","unstructured":"Listenberger LL, Brown DA (2007) Fluorescent detection of lipid droplets and associated proteins. Curr Protoc Cell Biol. Chapter 24:Unit 24.2. https:\/\/doi.org\/10.1002\/0471143030.cb2402s35","DOI":"10.1002\/0471143030.cb2402s35"},{"issue":"10","key":"1_CR26","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1016\/j.bbalip.2014.07.017","volume":"1842","author":"A Asimakopoulou","year":"2014","unstructured":"Asimakopoulou A, Borkham-Kamphorst E, Henning M, Yagmur E, Gassler N, Liedtke C, Berger T, Mak TW, Weiskirchen R (2014) Lipocalin-2 (LCN2) regulates PLIN5 expression and intracellular lipid droplet formation in the liver. Biochim Biophys Acta 1842(10):1513\u20131524. https:\/\/doi.org\/10.1016\/j.bbalip.2014.07.017","journal-title":"Biochim Biophys Acta"},{"key":"1_CR27","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1023\/A:1024184826728","volume":"248","author":"L Riccalton-Banks","year":"2003","unstructured":"Riccalton-Banks L, Bhandari R, Fry J, Shakesheff KM (2003) A simple method for the simultaneous isolation of stellate cells and hepatocytes from rat liver tissue. Mol Cell Biochem 248:97\u2013102. https:\/\/doi.org\/10.1023\/A:1024184826728","journal-title":"Mol Cell Biochem"},{"key":"1_CR28","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/978-1-4939-2815-6_1","volume":"1325","author":"I Mohar","year":"2015","unstructured":"Mohar I, Brempelis KJ, Murray SA, Ebrahimkhani MR, Crispe IN (2015) Isolation of non-parenchymal cells from the mouse liver. Methods Mol Biol 1325:3\u201317. https:\/\/doi.org\/10.1007\/978-1-4939-2815-6_1","journal-title":"Methods Mol Biol"},{"issue":"9","key":"1_CR29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138655","volume":"10","author":"M Werner","year":"2015","unstructured":"Werner M, Driftmann S, Kleinehr K, Kaiser GM, Math\u00e9 Z, Treckmann JW, Paul A, Skibbe K, Timm J, Canbay A, Gerken G, Schlaak JF, Broering R (2015) All-in-one: advanced preparation of human parenchymal and non-parenchymal liver cells. PLoS One 10(9):e0138655. https:\/\/doi.org\/10.1371\/journal.pone.0138655","journal-title":"PLoS One"},{"key":"1_CR30","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/978-1-61779-854-2_12","volume":"878","author":"D Davies","year":"2012","unstructured":"Davies D (2012) Cell separations by flow cytometry. Methods Mol Biol 878:185\u2013199. https:\/\/doi.org\/10.1007\/978-1-61779-854-2_12","journal-title":"Methods Mol Biol"},{"key":"1_CR31","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1111\/j.1582-4934.2007.00060.x","volume":"11","author":"J Herrmann","year":"2007","unstructured":"Herrmann J, Gressner AM, Weiskirchen R (2007) Immortal hepatic stellate cell lines: useful tools to study hepatic stellate cell biology and function? J Cell Mol Med 11:704\u2013722. https:\/\/doi.org\/10.1111\/j.1582-4934.2007.00060.x","journal-title":"J Cell Mol Med"},{"key":"1_CR32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056116","volume":"8","author":"SK Meurer","year":"2013","unstructured":"Meurer SK, Alsamman M, Sahin H, Wasmuth HE, Kisseleva T, Brenner DA, Trautwein C, Weiskirchen R, Scholten D (2013) Overexpression of endoglin modulates TGF-\u03b21-signalling pathways in a novel immortalized mouse hepatic stellate cell line. PLoS One 8:e56116. https:\/\/doi.org\/10.1371\/journal.pone.0056116","journal-title":"PLoS One"},{"issue":"3","key":"1_CR33","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1007\/s00418-008-0544-2","volume":"131","author":"E Van Rossen","year":"2009","unstructured":"Van Rossen E, Vander Borght S, van Grunsven LA, Reynaert H, Bruggeman V, Blomhoff R, Roskams T, Geerts A (2009) Vinculin and cellular retinol-binding protein-1 are markers for quiescent and activated hepatic stellate cells in formalin-fixed paraffin embedded human liver. Histochem Cell Biol. 131(3):313\u2013325. https:\/\/doi.org\/10.1007\/s00418-008-0544-2","journal-title":"Histochem Cell Biol."}],"container-title":["Methods in Molecular Biology","Hepatic Stellate Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3207-9_1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T08:04:57Z","timestamp":1685347497000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3207-9_1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"ISBN":["9781071632062","9781071632079"],"references-count":33,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3207-9_1","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]},"assertion":[{"value":"30 May 2023","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}