{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T12:32:09Z","timestamp":1793709129463,"version":"4.6.1"},"publisher-location":"New York, NY","reference-count":26,"publisher":"Springer US","isbn-type":[{"value":"9781071636657","type":"print"},{"value":"9781071636664","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-3666-4_4","type":"book-chapter","created":{"date-parts":[[2024,2,5]],"date-time":"2024-02-05T07:02:09Z","timestamp":1707116529000},"page":"43-70","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Production of Influenza Virus Glycoproteins Using Insect Cells"],"prefix":"10.1007","author":[{"given":"Madhumathi","family":"Loganathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Francis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Krammer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"4_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41572-018-0002-y","volume":"4","author":"F Krammer","year":"2018","unstructured":"Krammer F, Smith GJD, Fouchier RAM et al (2018) Influenza. Nat Rev Dis Primers 4:3. https:\/\/doi.org\/10.1038\/s41572-018-0002-y","journal-title":"Nat Rev Dis Primers"},{"key":"4_CR2","doi-asserted-by":"publisher","first-page":"1246","DOI":"10.1128\/JVI.02107-10","volume":"85","author":"S Khurana","year":"2011","unstructured":"Khurana S, Verma S, Verma N et al (2011) Bacterial HA1 vaccine against pandemic H5N1 influenza virus: evidence of oligomerization, hemagglutination, and cross-protective immunity in ferrets. J Virol 85:1246\u20131256. JVI.02107-10 [pii]. https:\/\/doi.org\/10.1128\/JVI.02107-10","journal-title":"J Virol"},{"key":"4_CR3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011694","volume":"5","author":"JM Aguilar-Y\u00e1\u00f1ez","year":"2010","unstructured":"Aguilar-Y\u00e1\u00f1ez JM, Portillo-Lara R, Mendoza-Ochoa GI et al (2010) An influenza a\/H1N1\/2009 hemagglutinin vaccine produced in Escherichia coli. PLoS One 5:e11694. https:\/\/doi.org\/10.1371\/journal.pone.0011694","journal-title":"PLoS One"},{"key":"4_CR4","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1046\/j.1432-1327.1999.00150.x","volume":"260","author":"X Saelens","year":"1999","unstructured":"Saelens X, Vanlandschoot P, Martinet W et al (1999) Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin. Eur J Biochem 260:166\u2013175","journal-title":"Eur J Biochem"},{"key":"4_CR5","doi-asserted-by":"publisher","first-page":"e43603","DOI":"10.1371\/journal.pone.0043603","volume":"7","author":"F Krammer","year":"2012","unstructured":"Krammer F, Margine I, Tan GS et al (2012) A carboxy-terminal trimerization domain stabilizes conformational epitopes on the stalk domain of soluble recombinant hemagglutinin substrates. PLoS One 7:e43603. PONE-D-12-16229 [pii]. https:\/\/doi.org\/10.1371\/journal.pone.0043603","journal-title":"PLoS One"},{"key":"4_CR6","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1126\/science.1243283","volume":"342","author":"JS McLellan","year":"2013","unstructured":"McLellan JS, Chen M, Joyce MG et al (2013) Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus. Science 342:592\u2013598. https:\/\/doi.org\/10.1126\/science.1243283","journal-title":"Science"},{"key":"4_CR7","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1126\/science.abd0826","volume":"369","author":"CL Hsieh","year":"2020","unstructured":"Hsieh CL, Goldsmith JA, Schaub JM et al (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes. Science 369:1501. https:\/\/doi.org\/10.1126\/science.abd0826","journal-title":"Science"},{"key":"4_CR8","doi-asserted-by":"publisher","first-page":"529","DOI":"10.2174\/0929866526666191112152646","volume":"27","author":"V Mishra","year":"2020","unstructured":"Mishra V (2020) A comprehensive guide to the commercial baculovirus expression vector systems for recombinant protein production. Protein Pept Lett 27:529\u2013537. https:\/\/doi.org\/10.2174\/0929866526666191112152646","journal-title":"Protein Pept Lett"},{"key":"4_CR9","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.molmed.2010.05.002","volume":"16","author":"F Krammer","year":"2010","unstructured":"Krammer F, Grabherr R (2010) Alternative influenza vaccines made by insect cells. Trends Mol Med 16:313\u2013320. S1471-4914(10)00071-7 [pii]. https:\/\/doi.org\/10.1016\/j.molmed.2010.05.002","journal-title":"Trends Mol Med"},{"key":"4_CR10","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.biologicals.2009.02.014","volume":"37","author":"MM Cox","year":"2009","unstructured":"Cox MM, Hollister JR (2009) FluBlok, a next generation influenza vaccine manufactured in insect cells. Biologicals 37:182\u2013189. https:\/\/doi.org\/10.1016\/j.biologicals.2009.02.014","journal-title":"Biologicals"},{"key":"4_CR11","doi-asserted-by":"publisher","unstructured":"Margine I, Palese P, Krammer F (2013) Expression of functional recombinant hemagglutinin and neuraminidase proteins from the novel H7N9 influenza virus using the baculovirus expression system. J Vis Exp. https:\/\/doi.org\/10.3791\/51112","DOI":"10.3791\/51112"},{"key":"4_CR12","doi-asserted-by":"publisher","first-page":"1866","DOI":"10.1126\/science.1093373","volume":"303","author":"J Stevens","year":"2004","unstructured":"Stevens J, Corper AL, Basler CF et al (2004) Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus. Science 303:1866\u20131870. 1093373 [pii]. https:\/\/doi.org\/10.1126\/science.1093373","journal-title":"Science"},{"key":"4_CR13","doi-asserted-by":"publisher","first-page":"e12466","DOI":"10.1371\/journal.pone.0012466","volume":"5","author":"WC Weldon","year":"2010","unstructured":"Weldon WC, Wang BZ, Martin MP et al (2010) Enhanced immunogenicity of stabilized trimeric soluble influenza hemagglutinin. PLoS One 5:e12466. https:\/\/doi.org\/10.1371\/journal.pone.0012466","journal-title":"PLoS One"},{"key":"4_CR14","doi-asserted-by":"publisher","first-page":"10493","DOI":"10.1128\/JVI.00959-08","volume":"82","author":"X Xu","year":"2008","unstructured":"Xu X, Zhu X, Dwek RA et al (2008) Structural characterization of the 1918 influenza virus H1N1 neuraminidase. J Virol 82:10493\u201310501. JVI.00959-08 [pii]. https:\/\/doi.org\/10.1128\/JVI.00959-08","journal-title":"J Virol"},{"key":"4_CR15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016284","volume":"6","author":"PM Schmidt","year":"2011","unstructured":"Schmidt PM, Attwood RM, Mohr PG et al (2011) A generic system for the expression and purification of soluble and stable influenza neuraminidase. PLoS One 6:e16284. https:\/\/doi.org\/10.1371\/journal.pone.0016284","journal-title":"PLoS One"},{"key":"4_CR16","doi-asserted-by":"publisher","first-page":"e0224121","DOI":"10.1128\/mBio.02241-21","volume":"12","author":"S Strohmeier","year":"2021","unstructured":"Strohmeier S, Amanat F, Zhu X et al (2021) A novel recombinant influenza virus neuraminidase vaccine candidate stabilized by a measles virus phosphoprotein tetramerization domain provides robust protection from virus challenge in the mouse model. mBio 12:e0224121. https:\/\/doi.org\/10.1128\/mBio.02241-21","journal-title":"mBio"},{"key":"4_CR17","doi-asserted-by":"publisher","first-page":"7129","DOI":"10.1016\/j.vaccine.2020.08.067","volume":"38","author":"M McMahon","year":"2020","unstructured":"McMahon M, Strohmeier S, Rajendran M et al (2020) Correctly folded \u2013 but not necessarily functional - influenza virus neuraminidase is required to induce protective antibody responses in mice. Vaccine 38:7129\u20137137. https:\/\/doi.org\/10.1016\/j.vaccine.2020.08.067","journal-title":"Vaccine"},{"key":"4_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/mBio.02332-17","volume":"9","author":"F Krammer","year":"2018","unstructured":"Krammer F, Fouchier RAM, Eichelberger MC et al (2018) NAction! How can neuraminidase-based immunity contribute to better influenza virus vaccines? MBio 9:1. https:\/\/doi.org\/10.1128\/mBio.02332-17","journal-title":"MBio"},{"key":"4_CR19","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.chroma.2006.09.067","volume":"1136","author":"T Dalakouras","year":"2006","unstructured":"Dalakouras T, Smith B, Platis D et al (2006) Development of recombinant protein-based influenza vaccine. Expression and affinity purification of H1N1 influenza virus neuraminidase. J Chromatogr A 1136:48\u201356. S0021-9673(06)01808-5 [pii]. https:\/\/doi.org\/10.1016\/j.chroma.2006.09.067","journal-title":"J Chromatogr A"},{"key":"4_CR20","doi-asserted-by":"publisher","first-page":"5496","DOI":"10.1016\/j.vaccine.2014.07.074","volume":"32","author":"B Buckland","year":"2014","unstructured":"Buckland B, Boulanger R, Fino M et al (2014) Technology transfer and scale-up of the Flublok recombinant hemagglutinin (HA) influenza vaccine manufacturing process. Vaccine 32:5496\u20135502. https:\/\/doi.org\/10.1016\/j.vaccine.2014.07.074","journal-title":"Vaccine"},{"key":"4_CR21","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1002\/biot.200900267","volume":"5","author":"F Krammer","year":"2010","unstructured":"Krammer F, Nakowitsch S, Messner P et al (2010) Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB\/c mice. Biotechnol J 5:17\u201323","journal-title":"Biotechnol J"},{"key":"4_CR22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051559","volume":"7","author":"I Margine","year":"2012","unstructured":"Margine I, Martinez-Gil L, Chou YY, Krammer F (2012) Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. PLoS One 7:e51559. https:\/\/doi.org\/10.1371\/journal.pone.0051559","journal-title":"PLoS One"},{"key":"4_CR23","doi-asserted-by":"publisher","first-page":"2847","DOI":"10.1128\/JVI.79.5.2847-2858.2005","volume":"79","author":"T Abe","year":"2005","unstructured":"Abe T, Hemmi H, Miyamoto H et al (2005) Involvement of the Toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus. J Virol 79:2847\u20132858","journal-title":"J Virol"},{"key":"4_CR24","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.4049\/jimmunol.171.3.1133","volume":"171","author":"T Abe","year":"2003","unstructured":"Abe T, Takahashi H, Hamazaki H et al (2003) Baculovirus induces an innate immune response and confers protection from lethal influenza virus infection in mice. J Immunol 171:1133\u20131139","journal-title":"J Immunol"},{"key":"4_CR25","doi-asserted-by":"publisher","first-page":"7629","DOI":"10.1128\/JVI.00679-09","volume":"83","author":"T Abe","year":"2009","unstructured":"Abe T, Kaname Y, Wen X et al (2009) Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner. J Virol 83:7629\u20137640. JVI.00679-09 [pii]. https:\/\/doi.org\/10.1128\/JVI.00679-09","journal-title":"J Virol"},{"key":"4_CR26","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1007\/s12033-010-9268-3","volume":"45","author":"F Krammer","year":"2010","unstructured":"Krammer F, Schinko T, Palmberger D et al (2010) Trichoplusia ni cells (High Five) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines. Mol Biotechnol 45:226\u2013234. https:\/\/doi.org\/10.1007\/s12033-010-9268-3","journal-title":"Mol Biotechnol"}],"container-title":["Methods in Molecular Biology","Recombinant Glycoproteins"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3666-4_4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T17:22:24Z","timestamp":1745688144000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3666-4_4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071636657","9781071636664"],"references-count":26,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3666-4_4","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"6 February 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}