{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:08:33Z","timestamp":1791320913792,"version":"4.3.1"},"publisher-location":"New York, NY","reference-count":26,"publisher":"Springer US","isbn-type":[{"value":"9781071636893","type":"print"},{"value":"9781071636909","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-3690-9_4","type":"book-chapter","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T11:03:37Z","timestamp":1710846217000},"page":"51-58","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Applications of ELISpot in the Identification and Treatment of Various Forms of Tuberculosis and in the Cancer Immunotherapies"],"prefix":"10.1007","author":[{"given":"Hemant K.","family":"Mishra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,3,20]]},"reference":[{"issue":"2","key":"4_CR1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1586\/17476348.2.2.253","volume":"2","author":"L Richeldi","year":"2008","unstructured":"Richeldi L, Losi M, Cerri S et al (2008) Using ELISpot technology to improve the diagnosis of tuberculosis infection: from the bench to the T-SPOT.TB assay. Expert Rev Respir Med 2(2):253\u2013260. https:\/\/doi.org\/10.1586\/17476348.2.2.253","journal-title":"Expert Rev Respir Med"},{"issue":"6","key":"4_CR2","doi-asserted-by":"publisher","first-page":"1944","DOI":"10.1128\/JCM.02265-05","volume":"44","author":"L Codecasa","year":"2006","unstructured":"Codecasa L, Mantegani P, Galli L et al (2006) An in-house RD1-based enzyme-linked immunospot-gamma interferon assay instead of the tuberculin skin test for diagnosis of latent Mycobacterium tuberculosis infection. J Clin Microbiol 44(6):1944\u20131950. https:\/\/doi.org\/10.1128\/JCM.02265-05","journal-title":"J Clin Microbiol"},{"issue":"10","key":"4_CR3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047340","volume":"7","author":"G Nkurunungi","year":"2012","unstructured":"Nkurunungi G, Lutangira JE, Lule SA et al (2012) Determining Mycobacterium tuberculosis infection among BCG-immunised Ugandan children by T-SPOT.TB and tuberculin skin testing. PLoS One 7(10):e47340. https:\/\/doi.org\/10.1371\/journal.pone.0047340","journal-title":"PLoS One"},{"key":"4_CR4","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/978-1-62703-727-3_5","volume":"1102","author":"LH Butterfield","year":"2014","unstructured":"Butterfield LH, Buffo MJ (2014) Immunologic monitoring of cancer vaccine trials using the ELISPOT assay. Methods Mol Biol 1102:71\u201382. https:\/\/doi.org\/10.1007\/978-1-62703-727-3_5","journal-title":"Methods Mol Biol"},{"issue":"Suppl 1","key":"4_CR5","doi-asserted-by":"publisher","first-page":"S1","DOI":"10.1016\/j.ijid.2022.02.057","volume":"124","author":"E Petersen","year":"2022","unstructured":"Petersen E, Al-Abri S, Chakaya J et al (2022) World TB day 2022: revamping and reshaping global TB control programs by advancing lessons learnt from the COVID-19 pandemic. Int J Infect Dis 124(Suppl 1):S1\u2013S3. https:\/\/doi.org\/10.1016\/j.ijid.2022.02.057","journal-title":"Int J Infect Dis"},{"issue":"3","key":"4_CR6","first-page":"335","volume":"10","author":"VK Singh","year":"2009","unstructured":"Singh VK, Chandra S, Kumar S et al (2009) A common medical error: lung cancer misdiagnosed as sputum negative tuberculosis. Asian Pac J Cancer Prev 10(3):335\u2013338","journal-title":"Asian Pac J Cancer Prev"},{"issue":"4","key":"4_CR7","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.eimc.2009.05.012","volume":"28","author":"A Lalvani","year":"2010","unstructured":"Lalvani A, Pareek M (2010) Interferon gamma release assays: principles and practice. Enferm Infecc Microbiol Clin 28(4):245\u2013252. https:\/\/doi.org\/10.1016\/j.eimc.2009.05.012","journal-title":"Enferm Infecc Microbiol Clin"},{"issue":"7","key":"4_CR8","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1089\/jir.2006.26.484","volume":"26","author":"SK Sharma","year":"2006","unstructured":"Sharma SK, Tahir M, Mohan A et al (2006) Diagnostic accuracy of ascitic fluid IFN-gamma and adenosine deaminase assays in the diagnosis of tuberculous ascites. J Interf Cytokine Res 26(7):484\u2013488. https:\/\/doi.org\/10.1089\/jir.2006.26.484","journal-title":"J Interf Cytokine Res"},{"issue":"5","key":"4_CR9","doi-asserted-by":"publisher","first-page":"416","DOI":"10.4046\/trd.2012.72.5.416","volume":"72","author":"K Cho","year":"2012","unstructured":"Cho K, Cho E, Kwon S et al (2012) Factors associated with indeterminate and false negative results of QuantiFERON-TB gold in-tube test in active tuberculosis. Tuberc Respir Dis (Seoul) 72(5):416\u2013425. https:\/\/doi.org\/10.4046\/trd.2012.72.5.416","journal-title":"Tuberc Respir Dis (Seoul)"},{"issue":"2","key":"4_CR10","doi-asserted-by":"publisher","first-page":"89","DOI":"10.3390\/cells1020089","volume":"1","author":"C Tincati","year":"2012","unstructured":"Tincati C, Cappione Iii AJ, Snyder-Cappione JE (2012) Distinguishing latent from active Mycobacterium tuberculosis infection using Elispot assays: looking beyond interferon-gamma. Cell 1(2):89\u201399. https:\/\/doi.org\/10.3390\/cells1020089","journal-title":"Cell"},{"issue":"3","key":"4_CR11","doi-asserted-by":"publisher","first-page":"229","DOI":"10.11150\/kansenshogakuzasshi.83.229","volume":"83","author":"S Murakami","year":"2009","unstructured":"Murakami S, Takeno M, Kobayashi M et al (2009) ELISPOT response to Mycobacterium tuberculosis antigens for diagnosing and monitoring tuberculosis patient therapy. Kansenshogaku Zasshi 83(3):229\u2013235. https:\/\/doi.org\/10.11150\/kansenshogakuzasshi.83.229","journal-title":"Kansenshogaku Zasshi"},{"issue":"12","key":"4_CR12","first-page":"1556","volume":"14","author":"M Zhang","year":"2010","unstructured":"Zhang M, Wang H, Liao M et al (2010) Diagnosis of latent tuberculosis infection in bacille Calmette-Guerin vaccinated subjects in China by interferon-gamma ELISpot assay. Int J Tuberc Lung Dis 14(12):1556\u20131563","journal-title":"Int J Tuberc Lung Dis"},{"issue":"6","key":"4_CR13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040192","volume":"4","author":"PC Hill","year":"2007","unstructured":"Hill PC, Brookes RH, Fox A et al (2007) Longitudinal assessment of an ELISpot test for Mycobacterium tuberculosis infection. PLoS Med 4(6):e192. https:\/\/doi.org\/10.1371\/journal.pmed.0040192","journal-title":"PLoS Med"},{"issue":"1","key":"4_CR14","doi-asserted-by":"publisher","first-page":"5","DOI":"10.3390\/cells1010005","volume":"1","author":"LS Sibley","year":"2012","unstructured":"Sibley LS, White AD, Marriott A et al (2012) ELISPOT refinement using spot morphology for assessing host responses to tuberculosis. Cell 1(1):5\u201314. https:\/\/doi.org\/10.3390\/cells1010005","journal-title":"Cell"},{"issue":"6","key":"4_CR15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0197825","volume":"13","author":"C Della Bella","year":"2018","unstructured":"Della Bella C, Spinicci M, Grassi A et al (2018) Novel M. tuberculosis specific IL-2 ELISpot assay discriminates adult patients with active or latent tuberculosis. PLoS One 13(6):e0197825. https:\/\/doi.org\/10.1371\/journal.pone.0197825","journal-title":"PLoS One"},{"issue":"4","key":"4_CR16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002790","volume":"16","author":"ZR Manjaly Thomas","year":"2019","unstructured":"Manjaly Thomas ZR, Satti I, Marshall JL et al (2019) Alternate aerosol and systemic immunisation with a recombinant viral vector for tuberculosis, MVA85A: a phase I randomised controlled trial. PLoS Med 16(4):e1002790. https:\/\/doi.org\/10.1371\/journal.pmed.1002790","journal-title":"PLoS Med"},{"issue":"2","key":"4_CR17","doi-asserted-by":"publisher","first-page":"111","DOI":"10.3390\/cells1020111","volume":"1","author":"AM Malyguine","year":"2012","unstructured":"Malyguine AM, Strobl S, Dunham K et al (2012) ELISpot assay for monitoring Cytotoxic T Lymphocytes (CTL) activity in cancer vaccine clinical trials. Cell 1(2):111\u2013126. https:\/\/doi.org\/10.3390\/cells1020111","journal-title":"Cell"},{"key":"4_CR18","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/978-1-4939-8567-8_5","volume":"1808","author":"J Hanson","year":"2018","unstructured":"Hanson J, Roen DR, Lehmann PV (2018) Four color ImmunoSpot((R)) assays for identification of effector T-cell lineages. Methods Mol Biol 1808:51\u201362. https:\/\/doi.org\/10.1007\/978-1-4939-8567-8_5","journal-title":"Methods Mol Biol"},{"key":"4_CR19","doi-asserted-by":"publisher","unstructured":"van Pul KM, Vuylsteke R, de Beijer MTA et al (2020) Breast cancer-induced immune suppression in the sentinel lymph node is effectively countered by CpG-B in conjunction with inhibition of the JAK2\/STAT3 pathway. J Immunother Cancer 8(2). https:\/\/doi.org\/10.1136\/jitc-2020-000761","DOI":"10.1136\/jitc-2020-000761"},{"key":"4_CR20","doi-asserted-by":"publisher","first-page":"1147","DOI":"10.3389\/fimmu.2020.01147","volume":"11","author":"E Podaza","year":"2020","unstructured":"Podaza E, Carri I, Aris M et al (2020) Evaluation of T-cell responses against shared melanoma associated antigens and predicted neoantigens in cutaneous melanoma patients treated with the CSF-470 allogeneic cell vaccine plus BCG and GM-CSF. Front Immunol 11:1147. https:\/\/doi.org\/10.3389\/fimmu.2020.01147","journal-title":"Front Immunol"},{"key":"4_CR21","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.778459","volume":"12","author":"J Bulgarelli","year":"2021","unstructured":"Bulgarelli J, Piccinini C, Petracci E et al (2021) Radiotherapy and high-dose Interleukin-2: clinical and immunological results of a proof of principle study in metastatic melanoma and renal cell carcinoma. Front Immunol 12:778459. https:\/\/doi.org\/10.3389\/fimmu.2021.778459","journal-title":"Front Immunol"},{"key":"4_CR22","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/bs.mie.2019.05.058","volume":"632","author":"D Negri","year":"2020","unstructured":"Negri D, Sestili P, Borghi M et al (2020) Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models. Methods Enzymol 632:457\u2013477. https:\/\/doi.org\/10.1016\/bs.mie.2019.05.058","journal-title":"Methods Enzymol"},{"key":"4_CR23","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1186\/1479-5876-6-81","volume":"6","author":"LH Butterfield","year":"2008","unstructured":"Butterfield LH, Disis ML, Fox BA et al (2008) A systematic approach to biomarker discovery; preamble to \u201cthe iSBTc-FDA taskforce on immunotherapy biomarkers\u201d. J Transl Med 6:81. https:\/\/doi.org\/10.1186\/1479-5876-6-81","journal-title":"J Transl Med"},{"issue":"4","key":"4_CR24","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1038\/nrc3237","volume":"12","author":"M Vanneman","year":"2012","unstructured":"Vanneman M, Dranoff G (2012) Combining immunotherapy and targeted therapies in cancer treatment. Nat Rev Cancer 12(4):237\u2013251. https:\/\/doi.org\/10.1038\/nrc3237","journal-title":"Nat Rev Cancer"},{"issue":"3","key":"4_CR25","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1586\/erv.10.169","volume":"10","author":"M Slota","year":"2011","unstructured":"Slota M, Lim JB, Dang Y et al (2011) ELISpot for measuring human immune responses to vaccines. Expert Rev Vaccines 10(3):299\u2013306. https:\/\/doi.org\/10.1586\/erv.10.169","journal-title":"Expert Rev Vaccines"},{"key":"4_CR26","doi-asserted-by":"publisher","unstructured":"Rodig SJ (2019) Detecting alkaline phosphatase-labeled cells. Cold Spring Harb Protoc 2019(4). https:\/\/doi.org\/10.1101\/pdb.prot099721","DOI":"10.1101\/pdb.prot099721"}],"container-title":["Methods in Molecular Biology","Handbook of ELISPOT"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3690-9_4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T11:10:13Z","timestamp":1710846613000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3690-9_4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071636893","9781071636909"],"references-count":26,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3690-9_4","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"20 March 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}