{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:08:07Z","timestamp":1775052487433,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":26,"publisher":"Springer US","isbn-type":[{"value":"9781071638415","type":"print"},{"value":"9781071638422","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-3842-2_13","type":"book-chapter","created":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T14:01:49Z","timestamp":1712325709000},"page":"177-187","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Protocol for the Automated Assessment of Cutaneous Pathology in a Mouse Model of Hemichannel Dysfunction"],"prefix":"10.1007","author":[{"given":"Chiara","family":"Peres","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Mammano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,4,6]]},"reference":[{"key":"13_CR1","doi-asserted-by":"publisher","DOI":"10.1093\/med\/9780190276478.001.0001","volume-title":"Genetic skin disorders. Oxford monographs on medical genetics","author":"VP Sybert","year":"2017","unstructured":"Sybert VP (2017) Genetic skin disorders. Oxford monographs on medical genetics. Oxford University Press"},{"key":"13_CR2","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.semcdb.2015.11.018","volume":"50","author":"E Lilly","year":"2016","unstructured":"Lilly E, Sellitto C, Milstone LM et al (2016) Connexin channels in congenital skin disorders. Semin Cell Dev Biol 50:4\u201312. https:\/\/doi.org\/10.1016\/j.semcdb.2015.11.018","journal-title":"Semin Cell Dev Biol"},{"issue":"3","key":"13_CR3","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1016\/j.jid.2015.11.017","volume":"136","author":"IE Garcia","year":"2016","unstructured":"Garcia IE, Bosen F, Mujica P et al (2016) From hyperactive Connexin26 Hemichannels to impairments in epidermal calcium gradient and permeability barrier in the keratitis-ichthyosis-deafness syndrome. J Invest Dermatol 136(3):574\u2013583. https:\/\/doi.org\/10.1016\/j.jid.2015.11.017","journal-title":"J Invest Dermatol"},{"issue":"1","key":"13_CR4","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.tcb.2021.07.007","volume":"32","author":"DW Laird","year":"2022","unstructured":"Laird DW, Lampe PD (2022) Cellular mechanisms of connexin-based inherited diseases. Trends Cell Biol 32(1):58\u201369. https:\/\/doi.org\/10.1016\/j.tcb.2021.07.007","journal-title":"Trends Cell Biol"},{"issue":"3","key":"13_CR5","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1016\/j.jaad.2018.09.042","volume":"80","author":"E Lilly","year":"2019","unstructured":"Lilly E, Bunick CG, Maley AM et al (2019) More than keratitis, ichthyosis, and deafness: multisystem effects of lethal GJB2 mutations. J Am Acad Dermatol 80(3):617\u2013625. https:\/\/doi.org\/10.1016\/j.jaad.2018.09.042","journal-title":"J Am Acad Dermatol"},{"key":"13_CR6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2023.105263","volume":"299","author":"SA Lucaciu","year":"2023","unstructured":"Lucaciu SA, Leighton SE, Hauser A et al (2023) Diversity in Connexin biology. J Biol Chem 299:105263. https:\/\/doi.org\/10.1016\/j.jbc.2023.105263","journal-title":"J Biol Chem"},{"issue":"15","key":"13_CR7","doi-asserted-by":"publisher","first-page":"2377","DOI":"10.1016\/j.yexcr.2010.05.026","volume":"316","author":"JC Saez","year":"2010","unstructured":"Saez JC, Schalper KA, Retamal MA et al (2010) Cell membrane permeabilization via connexin hemichannels in living and dying cells. Exp Cell Res 316(15):2377\u20132389. https:\/\/doi.org\/10.1016\/j.yexcr.2010.05.026","journal-title":"Exp Cell Res"},{"key":"13_CR8","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.phrs.2015.07.015","volume":"99","author":"NA Levit","year":"2015","unstructured":"Levit NA, White TW (2015) Connexin hemichannels influence genetically determined inflammatory and hyperproliferative skin diseases. Pharmacol Res 99:337\u2013343. https:\/\/doi.org\/10.1016\/j.phrs.2015.07.015","journal-title":"Pharmacol Res"},{"issue":"3 Pt 1","key":"13_CR9","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1038\/jid.2011.344","volume":"132","author":"G Burnstock","year":"2012","unstructured":"Burnstock G, Knight GE, Greig AV (2012) Purinergic signaling in healthy and diseased skin. J Invest Dermatol 132(3 Pt 1):526\u2013546. https:\/\/doi.org\/10.1038\/jid.2011.344","journal-title":"J Invest Dermatol"},{"issue":"16","key":"13_CR10","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.1093\/hmg\/ddh191","volume":"13","author":"GM Essenfelder","year":"2004","unstructured":"Essenfelder GM, Bruzzone R, Lamartine J et al (2004) Connexin30 mutations responsible for hidrotic ectodermal dysplasia cause abnormal hemichannel activity. Hum Mol Genet 13(16):1703\u20131714. https:\/\/doi.org\/10.1093\/hmg\/ddh191","journal-title":"Hum Mol Genet"},{"key":"13_CR11","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.ceca.2017.05.006","volume":"70","author":"F Mammano","year":"2018","unstructured":"Mammano F, Bortolozzi M (2018) Ca(2+) signaling, apoptosis and autophagy in the developing cochlea: milestones to hearing acquisition. Cell Calcium 70:117\u2013126. https:\/\/doi.org\/10.1016\/j.ceca.2017.05.006","journal-title":"Cell Calcium"},{"issue":"16","key":"13_CR12","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1039\/d0lc00427h","volume":"20","author":"F Mazzarda","year":"2020","unstructured":"Mazzarda F, D\u2019Elia A, Massari R et al (2020) Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca(2+) signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea. Lab Chip 20(16):3011\u20133023. https:\/\/doi.org\/10.1039\/d0lc00427h","journal-title":"Lab Chip"},{"key":"13_CR13","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.816079","volume":"16","author":"L Sun","year":"2022","unstructured":"Sun L, Gao D, Chen J et al (2022) Failure of hearing acquisition in Mice with reduced expression of Connexin 26 correlates with the abnormal phasing of apoptosis relative to autophagy and defective ATP-dependent Ca(2+) signaling in Kolliker\u2019s organ. Front Cell Neurosci 16:816079. https:\/\/doi.org\/10.3389\/fncel.2022.816079","journal-title":"Front Cell Neurosci"},{"issue":"2","key":"13_CR14","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1002\/ajmg.a.30515","volume":"133A","author":"AR Janecke","year":"2005","unstructured":"Janecke AR, Hennies HC, Gunther B et al (2005) GJB2 mutations in keratitis-ichthyosis-deafness syndrome including its fatal form. Am J Med Genet A 133A(2):128\u2013131. https:\/\/doi.org\/10.1002\/ajmg.a.30515","journal-title":"Am J Med Genet A"},{"issue":"7","key":"13_CR15","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1111\/j.1346-8138.2010.00839.x","volume":"37","author":"K Haruna","year":"2010","unstructured":"Haruna K, Suga Y, Oizumi A et al (2010) Severe form of keratitis-ichthyosis-deafness (KID) syndrome associated with septic complications. J Dermatol 37(7):680\u2013682. https:\/\/doi.org\/10.1111\/j.1346-8138.2010.00839.x","journal-title":"J Dermatol"},{"issue":"24","key":"13_CR16","doi-asserted-by":"publisher","first-page":"4776","DOI":"10.1091\/mbc.E11-09-0778","volume":"22","author":"G Mese","year":"2011","unstructured":"Mese G, Sellitto C, Li L et al (2011) The Cx26-G45E mutation displays increased hemichannel activity in a mouse model of the lethal form of keratitis-ichthyosis-deafness syndrome. Mol Biol Cell 22(24):4776\u20134786. https:\/\/doi.org\/10.1091\/mbc.E11-09-0778","journal-title":"Mol Biol Cell"},{"issue":"1","key":"13_CR17","doi-asserted-by":"publisher","first-page":"24118","DOI":"10.1038\/s41598-021-03627-8","volume":"11","author":"C Sellitto","year":"2021","unstructured":"Sellitto C, Li L, White TW (2021) Connexin hemichannel inhibition ameliorates epidermal pathology in a mouse model of keratitis ichthyosis deafness syndrome. Sci Rep 11(1):24118. https:\/\/doi.org\/10.1038\/s41598-021-03627-8","journal-title":"Sci Rep"},{"key":"13_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2023.104453","volume":"89","author":"C Peres","year":"2023","unstructured":"Peres C, Sellitto C, Nardin C et al (2023) Antibody gene transfer treatment drastically improves epidermal pathology in a keratitis ichthyosis deafness syndrome model using male mice. EBioMedicine 89:104453. https:\/\/doi.org\/10.1016\/j.ebiom.2023.104453","journal-title":"EBioMedicine"},{"issue":"3","key":"13_CR19","doi-asserted-by":"publisher","first-page":"1410","DOI":"10.1364\/boe.444525","volume":"13","author":"C Peres","year":"2022","unstructured":"Peres C, Nardin C, Yang G et al (2022) Commercially derived versatile optical architecture for two-photon STED, wavelength mixing and label-free microscopy. Biomed Opt Express 13(3):1410. https:\/\/doi.org\/10.1364\/boe.444525","journal-title":"Biomed Opt Express"},{"issue":"1","key":"13_CR20","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqab064","volume":"3","author":"V Donati","year":"2022","unstructured":"Donati V, Peres C, Nardin C et al (2022) Calcium signaling in the photodamaged skin: in vivo experiments and mathematical modeling. Function 3(1):zqab064. https:\/\/doi.org\/10.1093\/function\/zqab064","journal-title":"Function"},{"issue":"6","key":"13_CR21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000412","volume":"8","author":"C Kilkenny","year":"2010","unstructured":"Kilkenny C, Browne WJ, Cuthill IC et al (2010) Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol 8(6):e1000412. https:\/\/doi.org\/10.1371\/journal.pbio.1000412","journal-title":"PLoS Biol"},{"issue":"2","key":"13_CR22","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1177\/0023677217724823","volume":"52","author":"AJ Smith","year":"2018","unstructured":"Smith AJ, Clutton RE, Lilley E et al (2018) PREPARE: guidelines for planning animal research and testing. Lab Anim 52(2):135\u2013141. https:\/\/doi.org\/10.1177\/0023677217724823","journal-title":"Lab Anim"},{"issue":"3","key":"13_CR23","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1002\/jbmr.2374","volume":"30","author":"H Xu","year":"2015","unstructured":"Xu H, Gu S, Riquelme MA et al (2015) Connexin 43 channels are essential for normal bone structure and osteocyte viability. J Bone Miner Res 30(3):436\u2013448. https:\/\/doi.org\/10.1002\/jbmr.2374","journal-title":"J Bone Miner Res"},{"key":"13_CR24","doi-asserted-by":"publisher","first-page":"298","DOI":"10.3389\/fnmol.2017.00298","volume":"10","author":"L Xu","year":"2017","unstructured":"Xu L, Carrer A, Zonta F et al (2017) Design and characterization of a human monoclonal antibody that modulates mutant Connexin 26 Hemichannels implicated in deafness and skin disorders. Front Mol Neurosci 10:298. https:\/\/doi.org\/10.3389\/fnmol.2017.00298","journal-title":"Front Mol Neurosci"},{"key":"13_CR25","doi-asserted-by":"publisher","first-page":"392","DOI":"10.3389\/fphys.2019.00392","volume":"10","author":"G Ziraldo","year":"2019","unstructured":"Ziraldo G, Buratto D, Kuang Y et al (2019) A human-derived monoclonal antibody targeting extracellular Connexin domain selectively modulates Hemichannel function. Front Physiol 10:392. https:\/\/doi.org\/10.3389\/fphys.2019.00392","journal-title":"Front Physiol"},{"key":"13_CR26","doi-asserted-by":"publisher","unstructured":"Nardin C, Tettey-Matey A, Donati V et al (2022) A quantitative assay for Ca(2+) uptake through normal and pathological Hemichannels. Int J Mol Sci 23(13). https:\/\/doi.org\/10.3390\/ijms23137337","DOI":"10.3390\/ijms23137337"}],"container-title":["Methods in Molecular Biology","Connexin Hemichannels"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3842-2_13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,5]],"date-time":"2024-04-05T14:02:33Z","timestamp":1712325753000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3842-2_13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071638415","9781071638422"],"references-count":26,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3842-2_13","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"6 April 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}