{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:50:46Z","timestamp":1783349446871,"version":"3.54.6"},"publisher-location":"New York, NY","reference-count":38,"publisher":"Springer US","isbn-type":[{"value":"9781071639450","type":"print"},{"value":"9781071639467","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-3946-7_17","type":"book-chapter","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T04:01:44Z","timestamp":1719201704000},"page":"293-308","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Single-Cell MTT: A Simple and Sensitive Assay for Determining the Viability and Metabolic Activity of Polyploid Giant Cancer Cells (PGCCs)"],"prefix":"10.1007","author":[{"given":"Razmik","family":"Mirzayans","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonnie","family":"Andrais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Murray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"17_CR1","doi-asserted-by":"publisher","first-page":"360","DOI":"10.2174\/1568009618666180703154233","volume":"19","author":"J Chen","year":"2019","unstructured":"Chen J, Niu N, Zhang J, Qi L, Shen W, Donkena KV, Feng Z, Liu J (2019) Polyploid giant cancer cells (PGCCs): the evil roots of cancer. Curr Cancer Drug Targets 19:360\u2013367","journal-title":"Curr Cancer Drug Targets"},{"key":"17_CR2","doi-asserted-by":"publisher","first-page":"101","DOI":"10.3390\/genes13010101","volume":"13","author":"J Heng","year":"2022","unstructured":"Heng J, Heng HH (2022) Genome chaos, information creation, and cancer emergence: searching for new frameworks on the 50th anniversary of the \u201cwar on cancer\u201d. Genes 13:101","journal-title":"Genes"},{"key":"17_CR3","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.semcancer.2020.11.015","volume":"81","author":"E Sikora","year":"2022","unstructured":"Sikora E, Czarnecka-Herok J, Bojko A, Sunderland P (2022) Therapy-induced polyploidization and senescence: coincidence or interconnection? Semin Cancer Biol 81:83\u201395","journal-title":"Semin Cancer Biol"},{"key":"17_CR4","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1186\/1475-2867-13-92","volume":"13","author":"J Erenpreisa","year":"2013","unstructured":"Erenpreisa J, Cragg MS (2013) Three steps to the immortality of cancer cells: senescence, polyploidy and self-renewal. Cancer Cell Int 13:92","journal-title":"Cancer Cell Int"},{"key":"17_CR5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020838118","volume":"118","author":"KJ Pienta","year":"2021","unstructured":"Pienta KJ, Hammarlund EU, Brown JS, Amend SR, Axelrod RM (2021) Cancer recurrence and lethality are enabled by enhanced survival and reversible cell cycle arrest of polyaneuploid cells. Proc Natl Acad Sci USA 118:e2020838118","journal-title":"Proc Natl Acad Sci USA"},{"key":"17_CR6","doi-asserted-by":"publisher","first-page":"118","DOI":"10.3390\/cancers10040118","volume":"10","author":"R Mirzayans","year":"2018","unstructured":"Mirzayans R, Andrais B, Murray D (2018) Roles of polyploid\/multinucleated giant cancer cells in metastasis and disease relapse following anticancer treatment. Cancers 10:118","journal-title":"Cancers"},{"key":"17_CR7","doi-asserted-by":"publisher","first-page":"5766","DOI":"10.3390\/ijms21165766","volume":"21","author":"D Murray","year":"2020","unstructured":"Murray D, Mirzayans R (2020) Cellular responses to platinum-based anticancer drugs and UVC: role of p53 and implications for cancer therapy. Int J Mol Sci 21:5766","journal-title":"Int J Mol Sci"},{"key":"17_CR8","doi-asserted-by":"publisher","first-page":"3671","DOI":"10.3390\/cancers13153671","volume":"13","author":"V Zaitceva","year":"2021","unstructured":"Zaitceva V, Kopeina GS, Zhivotovsky B (2021) Anastasis: return journey from cell death. Cancers 13:3671","journal-title":"Cancers"},{"key":"17_CR9","doi-asserted-by":"publisher","first-page":"9090","DOI":"10.3390\/ijms21239090","volume":"21","author":"R Mirzayans","year":"2020","unstructured":"Mirzayans R, Murray D (2020) Do TUNEL and other apoptosis assays detect cell death in preclinical studies? Int J Mol Sci 21:9090","journal-title":"Int J Mol Sci"},{"key":"17_CR10","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.devcel.2004.08.019","volume":"7","author":"HD Ryoo","year":"2004","unstructured":"Ryoo HD, Gorenc T, Steller H (2004) Apoptotic cells can induce compensatory cell proliferation through the JNK and the wingless signaling pathways. Dev Cell 7:491\u2013501","journal-title":"Dev Cell"},{"key":"17_CR11","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/nrc.2016.58","volume":"16","author":"G Ichim","year":"2016","unstructured":"Ichim G, Tait SW (2016) A fate worse than death: apoptosis as an oncogenic process. Nat Rev Cancer 16:539\u2013548","journal-title":"Nat Rev Cancer"},{"key":"17_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107731","volume":"31","author":"K Berthenet","year":"2020","unstructured":"Berthenet K, Castillo Ferrer C, Fanfone D, Popgeorgiev N, Neves D, Bertolino P, Gibert B, Hernandez-Vargas H, Ichim G (2020) Failed apoptosis enhances melanoma cancer cell aggressiveness. Cell Rep 31:107731","journal-title":"Cell Rep"},{"key":"17_CR13","doi-asserted-by":"publisher","first-page":"2240","DOI":"10.1091\/mbc.e11-11-0926","volume":"23","author":"HL Tang","year":"2012","unstructured":"Tang HL, Tang HM, Mak KH, Hu S, Wang SS, Wong KM, Wong CST, Wu HY, Law HT, Liu K et al (2012) Cell survival, DNA damage, and oncogenic transformation after a transient and reversible apoptotic response. Mol Biol Cell 23:2240\u20132252","journal-title":"Mol Biol Cell"},{"key":"17_CR14","doi-asserted-by":"publisher","first-page":"43","DOI":"10.12688\/f1000research.10568.1","volume":"6","author":"HM Tang","year":"2017","unstructured":"Tang HM, Talbot CC Jr, Fung MC, Tang HL (2017) Molecular signature of anastasis for reversal of apoptosis. F1000Research 6:43","journal-title":"F1000Research"},{"key":"17_CR15","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.3390\/ijms23031152","volume":"23","author":"F Corsi","year":"2022","unstructured":"Corsi F, Capradossi F, Pelliccia A, Briganti S, Bruni E, Traversa E, Torino F, Reichle A, Ghibelli (2022) Apoptosis as driver of therapy-induced cancer repopulation and acquired cell-resistance (CRAC): a simple in vitro model of Phoenix Rising in prostate cancer. Int J Mol Sci 23:1152","journal-title":"Int J Mol Sci"},{"key":"17_CR16","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.676344","volume":"9","author":"JC Ye","year":"2021","unstructured":"Ye JC, Horne S, Zhang JZ, Jackson L, Heng HH (2021) Therapy induced genome chaos: a novel mechanism of rapid cancer drug resistance. Front Cell Dev Biol 9:676344","journal-title":"Front Cell Dev Biol"},{"key":"17_CR17","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.3390\/ijms21041308","volume":"21","author":"R Mirzayans","year":"2020","unstructured":"Mirzayans R, Murray D (2020) Intratumor heterogeneity and therapy resistance: contributions of dormancy, apoptosis reversal (anastasis) and cell fusion to disease recurrence. Int J Mol Sci 21:1308","journal-title":"Int J Mol Sci"},{"key":"17_CR18","doi-asserted-by":"publisher","first-page":"8854","DOI":"10.18632\/oncotarget.12673","volume":"8","author":"A Eastman","year":"2017","unstructured":"Eastman A (2017) Improving anticancer drug development begins with cell culture: misinformation perpetrated by the misuse of cytotoxicity assays. Oncotarget 8:8854\u20138866","journal-title":"Oncotarget"},{"key":"17_CR19","doi-asserted-by":"publisher","first-page":"255","DOI":"10.3390\/cancers10080255","volume":"10","author":"R Mirzayans","year":"2018","unstructured":"Mirzayans R, Andrais B, Murray D (2018) Viability assessment following anticancer treatment requires single-cell visualization. Cancers 10:255","journal-title":"Cancers"},{"key":"17_CR20","doi-asserted-by":"publisher","first-page":"13266","DOI":"10.1073\/pnas.95.22.13266","volume":"95","author":"Y Liu","year":"1988","unstructured":"Liu Y, Peterson DA, Schubert D (1988) Amyloid \u03b2 peptide alters intracellular vesicle trafficking and cholesterol homeostasis. Proc Natl Acad Sci USA 95:13266\u201313271","journal-title":"Proc Natl Acad Sci USA"},{"key":"17_CR21","doi-asserted-by":"publisher","first-page":"1531","DOI":"10.1038\/s41598-018-19930-w","volume":"8","author":"Y Rai","year":"2018","unstructured":"Rai Y, Pathak R, Kumari N, Sah DK, Pandey S, Kalra N, Soni R, Dwarakanath BS, Bhatt AN (2018) Mitochondrial biogenesis and metabolic hyperactivation limits the application of MTT assay in the estimation of radiation induced growth inhibition. Sci Rep 8:1531","journal-title":"Sci Rep"},{"key":"17_CR22","doi-asserted-by":"publisher","first-page":"360","DOI":"10.3390\/ijms18020360","volume":"18","author":"R Mirzayans","year":"2017","unstructured":"Mirzayans R, Andrais B, Scott A, Wang YW, Kumar P, Murray D (2017) Multinucleated giant cancer cells produced in response to ionizing radiation retain viability and replicate their genome. Int J Mol Sci 18:360","journal-title":"Int J Mol Sci"},{"key":"17_CR23","doi-asserted-by":"publisher","first-page":"1460","DOI":"10.3390\/ijms18071460","volume":"18","author":"R Mirzayans","year":"2017","unstructured":"Mirzayans R, Andrais B, Murray D (2017) Impact of premature senescence on radiosensitivity measured by high throughput cell-based assays. Int J Mol Sci 18:1460","journal-title":"Int J Mol Sci"},{"key":"17_CR24","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.3390\/ijms18081679","volume":"18","author":"R Mirzayans","year":"2017","unstructured":"Mirzayans R, Andrais B, Murray D (2017) Do multiwell plate high throughput assays measure loss of cell viability following exposure to genotoxic agents? Int J Mol Sci 18:1679","journal-title":"Int J Mol Sci"},{"issue":"2","key":"17_CR25","first-page":"1118","volume":"6","author":"R Mirzayans","year":"2018","unstructured":"Mirzayans R, Andrais B, Murray D (2018) Impact of chemotherapeutic drugs on cancer cell proliferation, morphology and metabolic activity. J Cancer Biol Res 6(2):1118","journal-title":"J Cancer Biol Res"},{"key":"17_CR26","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-0716-2376-3_3","volume":"2508","author":"V Noel","year":"2022","unstructured":"Noel V, Berry MD (2022) Culture of adherent cancer cell lines. Methods Mol Biol 2508:19\u201329","journal-title":"Methods Mol Biol"},{"key":"17_CR27","first-page":"A3.B.1","volume":"111","author":"W Storber","year":"2019","unstructured":"Storber W (2019) Trypan blue exclusion test of cell viability. Curr Protoc Immunol 111:A3.B.1\u2013A3.B.3","journal-title":"Curr Protoc Immunol"},{"key":"17_CR28","doi-asserted-by":"publisher","first-page":"963","DOI":"10.1038\/cdd.2013.27","volume":"20","author":"M Husmann","year":"2013","unstructured":"Husmann M (2013) Vital dyes and virtual deaths. Cell Death Differ 20:963","journal-title":"Cell Death Differ"},{"key":"17_CR29","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1038\/s41421-020-00233-2","volume":"7","author":"C Dias","year":"2021","unstructured":"Dias C, Nylandsted J (2021) Plasma membrane integrity in health and disease: significance and therapeutic potential. Cell Discov 7:4","journal-title":"Cell Discov"},{"key":"17_CR30","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1590\/1414-431X20143437","volume":"47","author":"BA Avelar-Freitas","year":"2014","unstructured":"Avelar-Freitas BA, Almeida VG, Pinto MCX, Mourao FAG, Massensini AR, Martins-Filho OA, Rocha-Vieira E, Brito-Melo GEA (2014) Trypan blue exclusion assay by flow cytometry. Braz J Med Biol Res 47:307\u2013315","journal-title":"Braz J Med Biol Res"},{"key":"17_CR31","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1016\/j.cellbi.2008.04.021","volume":"32","author":"PE Puig","year":"2008","unstructured":"Puig PE, Guilly MN, Bouchot A, Droin N, Cathelin D, Bouyer F, Favier L, Ghiringhelli F, Kroemer G, Solary E et al (2008) Tumor cells can escape DNA-damaging cisplatin through DNA endoreduplication and reversible polyploidy. Cell Biol Int 32:1031\u20131043","journal-title":"Cell Biol Int"},{"key":"17_CR32","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/s00280-022-04400-y","volume":"89","author":"RE Nicoletto","year":"2022","unstructured":"Nicoletto RE, Ofner CM III (2022) Cytotoxic mechanisms of doxorubicin at clinically relevant concentrations in breast cancer cells. Cancer Chemother Pharmacol 89:285\u2013311","journal-title":"Cancer Chemother Pharmacol"},{"key":"17_CR33","doi-asserted-by":"publisher","first-page":"11935","DOI":"10.1038\/s41598-018-29817-5","volume":"8","author":"B Xuan","year":"2018","unstructured":"Xuan B, Ghosh D, Cheney EM, Clifton EM, Dawson MR (2018) Dysregulation in actin cytoskeletal organization drives increased stiffness and migratory persistence in polyploidal giant cancer cells. Sci Rep 8:11935","journal-title":"Sci Rep"},{"key":"17_CR34","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.ymeth.2013.01.008","volume":"61","author":"E Hollville","year":"2013","unstructured":"Hollville E (2013) Measuring apoptosis by microscopy and fow cytometry. Methods 61:90\u201397","journal-title":"Methods"},{"key":"17_CR35","doi-asserted-by":"publisher","first-page":"2654","DOI":"10.1021\/acssensors.9b01061","volume":"4","author":"M Wei","year":"2019","unstructured":"Wei M, Zhang R, Zhang F, Zhang Y, Li G, Miao R, Shao S (2019) An evaluation approach of cell viability based on cell detachment assay in a single-channel integrated microfluidic chip. ACS Sens 4:2654\u20132661","journal-title":"ACS Sens"},{"key":"17_CR36","doi-asserted-by":"publisher","first-page":"8288","DOI":"10.3390\/ijms23158288","volume":"23","author":"J Czarnecka-Herok","year":"2022","unstructured":"Czarnecka-Herok J, Sliwinska MA, Herok M, Targonska A, Strzeszewska-Potyrala A, Bojko A, Wolny A, Mosieniak G, Sikora E (2022) Therapy-induced senescent\/polyploid cancer cells undergo atypical divisions associated with altered expression of meiosis, spermatogenesis and EMT genes. Int J Mol Sci 23:8288","journal-title":"Int J Mol Sci"},{"key":"17_CR37","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/j.cell.2019.05.041","volume":"178","author":"CH Hsu","year":"2019","unstructured":"Hsu CH, Altschuler SJ, Wu LF (2019) Patterns of early p21 dynamics determine proliferation-senescence cell fate after chemotherapy. Cell 178:361\u2013373","journal-title":"Cell"},{"key":"17_CR38","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1667\/RR3280","volume":"163","author":"R Mirzayans","year":"2005","unstructured":"Mirzayans R, Scott A, Cameron M, Murray D (2005) Induction of accelerated senescence following exposure to ionizing radiation in human solid tumor-derived cell lines expressing wild-type TP53. Radiat Res 163:53\u201362","journal-title":"Radiat Res"}],"container-title":["Methods in Molecular Biology","Cancer Cytogenetics and Cytogenomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3946-7_17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T04:03:20Z","timestamp":1719201800000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3946-7_17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071639450","9781071639467"],"references-count":38,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3946-7_17","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"25 June 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}