{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T20:18:15Z","timestamp":1778789895569,"version":"3.51.4"},"publisher-location":"New York, NY","reference-count":64,"publisher":"Springer US","isbn-type":[{"value":"9781071639535","type":"print"},{"value":"9781071639542","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-3954-2_5","type":"book-chapter","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T13:03:08Z","timestamp":1720616588000},"page":"71-84","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Use of Electron Microscopy for the Detection of Contaminant Endophytic Bacteria In Vitro"],"prefix":"10.1007","author":[{"given":"Ina\u00ea Mari\u00ea","family":"de Ara\u00fajo Silva-Cardoso","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Oliveira","family":"Medeiros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Cristina Meneses Mendes","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Luis Xavier","family":"de Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonny Everson","family":"Scherwinski-Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,11]]},"reference":[{"key":"5_CR1","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1007\/s11240-022-02360-y","volume":"151","author":"HG Bao","year":"2022","unstructured":"Bao HG, Tung HT, Van HT et al (2022) Copper nanoparticles enhanced surface disinfection, induction and maturation of somatic embryos in tuberous begonias (Begonia \u00d7 tuberhybrida Voss) cultured in vitro. Plant Cell Tissue Organ Cult 151:385\u2013399. https:\/\/doi.org\/10.1007\/s11240-022-02360-y","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR2","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/BF00303371","volume":"37","author":"C Leifert","year":"2001","unstructured":"Leifert C, Cassells AC (2001) Microbial hazards in plant tissue and cell cultures. In Vitro Cell Dev Biol Plant 37:133\u2013138. https:\/\/doi.org\/10.1007\/BF00303371","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR3","doi-asserted-by":"publisher","unstructured":"Cobrado J, Fernandez A (2016) Common fungi contamination affecting tissue-cultured Abaca (Musa textiles Nee) during initial stage of micropropagation. Asian Res J Agric 1:1\u20137. https:\/\/doi.org\/10.9734\/ARJA\/2016\/28353","DOI":"10.9734\/ARJA\/2016\/28353"},{"key":"5_CR4","doi-asserted-by":"publisher","first-page":"875","DOI":"10.9755\/ejfa.v25i11.15351","volume":"25","author":"MH Abass","year":"2013","unstructured":"Abass MH (2013) Microbial contaminants of date palm (Phoenix dactylifera L.) in Iraqi tissue culture laboratories. Emirates J Food Agric 25:875\u2013882. https:\/\/doi.org\/10.9755\/ejfa.v25i11.15351","journal-title":"Emirates J Food Agric"},{"key":"5_CR5","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1007\/s11738-018-2727-3","volume":"40","author":"T Khan","year":"2018","unstructured":"Khan T, Abbasi BH, Iqrar I et al (2018) Molecular identification and control of endophytic contamination during in vitro plantlet development of Fagonia indica. Acta Physiol Plant 40:150. https:\/\/doi.org\/10.1007\/s11738-018-2727-3","journal-title":"Acta Physiol Plant"},{"key":"5_CR6","doi-asserted-by":"publisher","DOI":"10.1016\/j.rhisph.2022.100613","volume":"24","author":"F Doni","year":"2022","unstructured":"Doni F, Miranti M, Mispan MS et al (2022) Multi-omics approaches for deciphering the microbial modulation of plants\u2019 genetic potentials: what\u2019s known and what\u2019s next? Rhizosphere 24:100613. https:\/\/doi.org\/10.1016\/j.rhisph.2022.100613","journal-title":"Rhizosphere"},{"key":"5_CR7","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/BF02318961","volume":"47","author":"AC Cassells","year":"1996","unstructured":"Cassells AC, Tahmatsidou V (1996) The influence of local plant growth conditions on non-fastidious bacterial contamination of meristem-tips of Hydrangea cultured in vitro. Plant Cell Tissue Organ Cult 47:15\u201326. https:\/\/doi.org\/10.1007\/BF02318961","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR8","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1007\/BF00303371","volume":"7","author":"C Leifert","year":"1991","unstructured":"Leifert C, Ritchie JY, Waites WM (1991) Contaminants of plant-tissue and cell cultures. World J Microbiol Biotechnol 7:452\u2013469. https:\/\/doi.org\/10.1007\/BF00303371","journal-title":"World J Microbiol Biotechnol"},{"issue":"5","key":"5_CR9","doi-asserted-by":"publisher","DOI":"10.1002\/aps3.11489","volume":"10","author":"GM Volk","year":"2022","unstructured":"Volk GM, Bonnart R, Oliveira ACA et al (2022) Minimizing the deleterious effects of endophytes in plant shoot tip cryopreservation. Appl Plant Sci 10(5):e11489. https:\/\/doi.org\/10.1002\/aps3.11489","journal-title":"Appl Plant Sci"},{"key":"5_CR10","first-page":"348","volume":"53","author":"UE Okoroafor","year":"2022","unstructured":"Okoroafor UE (2022) Microbial contamination in plant tissue culture and elimination strategies. Niger Agric J 53:348\u2013355","journal-title":"Niger Agric J"},{"key":"5_CR11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/1678-4324-2016160485","volume":"60","author":"SS Ray","year":"2017","unstructured":"Ray SS, Ali N (2017) Biotic contamination and possible ways of sterilization: a review with reference to bamboo micropropagation. Braz Arch Biol Technol 60:1\u201312. https:\/\/doi.org\/10.1590\/1678-4324-2016160485","journal-title":"Braz Arch Biol Technol"},{"key":"5_CR12","first-page":"111","volume":"4","author":"EI Eziashi","year":"2014","unstructured":"Eziashi EI, Asemota O, Okwuagwu CO et al (2014) Screening sterilizing agents and antibiotics for the elimination of bacterial contaminants from oil palm explants for plant tissue culture. Eur J Exp Biol 4:111\u2013115","journal-title":"Eur J Exp Biol"},{"key":"5_CR13","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s11240-012-0129-6","volume":"110","author":"JY Fang","year":"2012","unstructured":"Fang JY, Hsu YR (2012) Molecular identification and antibiotic control of endophytic bacterial contaminants from micropropagated Aglaonema cultures. Plant Cell Tissue Organ Cult 110:53\u201362. https:\/\/doi.org\/10.1007\/s11240-012-0129-6","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR14","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.micron.2005.10.001","volume":"37","author":"A Curry","year":"2006","unstructured":"Curry A, Appleton H, Dowsett B (2006) Application of transmission electron microscopy to the clinical study of viral and bacterial infections: present and future. Micron 37:91\u2013106. https:\/\/doi.org\/10.1016\/j.micron.2005.10.001","journal-title":"Micron"},{"key":"5_CR15","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1111\/ppl.13026","volume":"168","author":"IM Ara\u00fajo Silva-Cardoso","year":"2020","unstructured":"Ara\u00fajo Silva-Cardoso IM, Meira FS, Gomes ACMM et al (2020) Histology, histochemistry and ultrastructure of pre-embryogenic cells determined for direct somatic embryogenesis in the palm tree Syagrus oleracea. Physiol Plant 168:845\u2013875. https:\/\/doi.org\/10.1111\/ppl.13026","journal-title":"Physiol Plant"},{"key":"5_CR16","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s11240-008-9413-x","volume":"95","author":"AM Pirttil\u00e4","year":"2008","unstructured":"Pirttil\u00e4 AM, Podolich O, Koskim\u00e4ki JJ et al (2008) Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.). Plant Cell Tissue Organ Cult 95:47\u201355. https:\/\/doi.org\/10.1007\/s11240-008-9413-x","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR17","doi-asserted-by":"publisher","first-page":"plt011","DOI":"10.1093\/aobpla\/plt011","volume":"5","author":"P Thomas","year":"2013","unstructured":"Thomas P, Reddy KM (2013) Microscopic elucidation of abundant endophytic bacteria colonizing the cell wall-plasma membrane peri-space in the shoot-tip tissue of banana. AoB Plants 5:plt011. https:\/\/doi.org\/10.1093\/aobpla\/plt011","journal-title":"AoB Plants"},{"key":"5_CR18","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/s00284-016-1163-0","volume":"74","author":"NP Esposito-Polesi","year":"2017","unstructured":"Esposito-Polesi NP, Abreu-Tarazi MF, Almeida CV et al (2017) Investigation of endophytic bacterial community in supposedly axenic cultures of pineapple and orchids with evidence on abundant intracellular bacteria. Curr Microbiol 74:103\u2013113. https:\/\/doi.org\/10.1007\/s00284-016-1163-0","journal-title":"Curr Microbiol"},{"issue":"12","key":"5_CR19","doi-asserted-by":"publisher","first-page":"526","DOI":"10.3390\/horticulturae7120526","volume":"7","author":"AN El-Banna","year":"2021","unstructured":"El-Banna AN, El-Mahrouk ME, Dewir YH et al (2021) Endophytic bacteria in banana in vitro cultures: molecular identification, antibiotic susceptibility, and plant survival. Horticulturae 7(12):526. https:\/\/doi.org\/10.3390\/horticulturae7120526","journal-title":"Horticulturae"},{"key":"5_CR20","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s11240-016-1144-9","volume":"128","author":"T Orlikowska","year":"2017","unstructured":"Orlikowska T, Nowak K, Reed B (2017) Bacteria in the plant tissue culture environment. Plant Cell Tissue Organ Cult 128:487\u2013508. https:\/\/doi.org\/10.1007\/s11240-016-1144-9","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR21","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/978-1-61779-818-4_6","volume":"877","author":"AC Cassells","year":"2012","unstructured":"Cassells AC (2012) Pathogen and biological contamination management in plant tissue culture: phytopathogens, vitro pathogens, and vitro pests. Mol Biol 877:57\u201380. https:\/\/doi.org\/10.1007\/978-1-61779-818-4_6","journal-title":"Mol Biol"},{"key":"5_CR22","first-page":"223","volume-title":"Plant tissue culture, development, and biotechnology","author":"AC Cassells","year":"2011","unstructured":"Cassells AC (2011) Detection and elimination of microbial endophytes and prevention of contamination in plant tissue culture. In: Trigiano RN, Gray DJ (eds) Plant tissue culture, development, and biotechnology. CRC Press, Boca Raton, pp 223\u2013238"},{"key":"5_CR23","doi-asserted-by":"publisher","first-page":"6431301","DOI":"10.1155\/2020\/6431301","volume":"2020","author":"S Tewelde","year":"2020","unstructured":"Tewelde S, Patharajan S, Teka Z et al (2020) Assessing the efficacy of broad-spectrum antibiotics in controlling bacterial contamination in the in vitro micropropagation of ginger (Zingiber officinale Rosc). Sci World J 2020:6431301. https:\/\/doi.org\/10.1155\/2020\/6431301","journal-title":"Sci World J"},{"key":"5_CR24","doi-asserted-by":"publisher","first-page":"7581","DOI":"10.1016\/j.sjbs.2021.09.064","volume":"28","author":"S Rafiq","year":"2021","unstructured":"Rafiq S, Rather ZA, Bhat RA et al (2021) Standardization of in vitro micropropagation procedure of Oriental Lilium Hybrid Cv. \u2018Ravenna\u2019. Saudi J Biol Sci 28:7581\u20137587. https:\/\/doi.org\/10.1016\/j.sjbs.2021.09.064","journal-title":"Saudi J Biol Sci"},{"issue":"1","key":"5_CR25","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s13568-022-01343-8","volume":"12","author":"F Ahmadpoor","year":"2022","unstructured":"Ahmadpoor F, Zare N, Asghari R et al (2022) Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L. AMB Express 12(1):3. https:\/\/doi.org\/10.1186\/s13568-022-01343-8","journal-title":"AMB Express"},{"key":"5_CR26","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1007\/s11627-014-9659-4","volume":"51","author":"Bhawana","year":"2015","unstructured":"Bhawana, Stubblefield JM, Newsome AL et al (2015) Surface decontamination of plant tissue explants with chlorine dioxide gas. In Vitro Cell Dev Biol Plant 51:214\u2013219. https:\/\/doi.org\/10.1007\/s11627-014-9659-4","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR27","doi-asserted-by":"publisher","first-page":"10232","DOI":"10.1038\/s41598-019-46795-4","volume":"9","author":"Y Duan","year":"2019","unstructured":"Duan Y, Zhang H, Sun M et al (2019) Use of chlorine dioxide to sterilize medium for tissue culture of potato. Sci Rep 9:10232. https:\/\/doi.org\/10.1038\/s41598-019-46795-4","journal-title":"Sci Rep"},{"key":"5_CR28","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1007\/s11240-019-01681-9","volume":"139","author":"CC Urtiga","year":"2019","unstructured":"Urtiga CC, Silva-Cardoso IMA, Figueiredo SA (2019) Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture. Plant Cell Tissue Organ Cult 139:601\u2013604. https:\/\/doi.org\/10.1007\/s11240-019-01681-9","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR29","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1007\/s42452-020-03948-9","volume":"2","author":"I And\u00fajar","year":"2020","unstructured":"And\u00fajar I, Gonz\u00e1lez N, Garc\u00eda-Ramos JC et al (2020) ArgovitTM silver nanoparticles reduce contamination levels and improve morphological growth in the in vitro culture of Psidium friedrichsthalianum (O. Berg) Nied. SN Appl Sci 2:2110. https:\/\/doi.org\/10.1007\/s42452-020-03948-9","journal-title":"SN Appl Sci"},{"key":"5_CR30","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/BF02632228","volume":"31","author":"BM Reed","year":"1995","unstructured":"Reed BM, Buckley PM, Dewilde TN et al (1995) Detection and eradication of endophytic bacteria from micropropagated mint plants. In Vitro Cell Dev Biol Plant 31:53\u201357. https:\/\/doi.org\/10.1007\/BF02632228","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR31","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1007\/s11240-016-1104-4","volume":"128","author":"SB Javed","year":"2017","unstructured":"Javed SB, Alatar AA, Basahi R et al (2017) Copper induced suppression of systemic microbial contamination in Erythrina variegata L. during in vitro culture. Plant Cell Tissue Organ Cult 128:249\u2013258. https:\/\/doi.org\/10.1007\/s11240-016-1104-4","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR32","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/978-94-011-0790-7_43","volume-title":"Physiology, growth and development of plants in culture","author":"HE Gunson","year":"1994","unstructured":"Gunson HE, Spencer-Phillips PTN (1994) Latent bacterial infections: epiphytes and endophytes as contaminants of micropropagated plants. In: Lumsden PJ, Nicholas LR, Davies WL (eds) Physiology, growth and development of plants in culture. Springer, Dordrecht, pp 379\u2013396. https:\/\/doi.org\/10.1007\/978-94-011-0790-7_43"},{"key":"5_CR33","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1007\/s13205-018-1378-9","volume":"8","author":"K Alagarsamy","year":"2018","unstructured":"Alagarsamy K, Shamala LF, Wei S (2018) Influence of media supplements on inhibition of oxidative browning and bacterial endophytes of Camellia sinensis var. Sinensis. 3 Biotech 8:356. https:\/\/doi.org\/10.1007\/s13205-018-1378-9","journal-title":"3 Biotech"},{"key":"5_CR34","doi-asserted-by":"publisher","first-page":"3073","DOI":"10.1128\/AEM.66.7.3073-3077.2000","volume":"66","author":"AM Pirttil\u00e4","year":"2000","unstructured":"Pirttil\u00e4 AM, Laukkanen H, Pospiech H et al (2000) Detection of intracellular bacteria in the buds of scotch pine (Pinus sylvestris L.) by in situ hybridization. Appl Environ Microbiol 66:3073\u20133077. https:\/\/doi.org\/10.1128\/AEM.66.7.3073-3077.2000","journal-title":"Appl Environ Microbiol"},{"key":"5_CR35","doi-asserted-by":"publisher","first-page":"1757","DOI":"10.1007\/s11274-009-0073-8","volume":"25","author":"CV Almeida","year":"2009","unstructured":"Almeida CV, Andreote FD, Yara R et al (2009) Bacteriosomes in axenic plants: endophytes as stable endosymbionts. World J Microbiol Biotechnol 25:1757\u20131764. https:\/\/doi.org\/10.1007\/s11274-009-0073-8","journal-title":"World J Microbiol Biotechnol"},{"issue":"2","key":"5_CR36","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3390\/microorganisms9020269","volume":"9","author":"P Thomas","year":"2021","unstructured":"Thomas P, Franco CMM (2021) Intracellular bacteria in plants: elucidation of abundant and diverse cytoplasmic bacteria in healthy plant cells using in vitro cell and callus cultures. Microorganisms 9(2):269. https:\/\/doi.org\/10.3390\/microorganisms9020269","journal-title":"Microorganisms"},{"issue":"4","key":"5_CR37","doi-asserted-by":"publisher","first-page":"453","DOI":"10.3390\/horticulturae9040453","volume":"9","author":"N Permadi","year":"2023","unstructured":"Permadi N, Nurzaman M, Alhasnawi AN et al (2023) Managing lethal browning and microbial contamination in Musa spp. Tissue culture: synthesis and perspectives. Horticulturae 9(4):453. https:\/\/doi.org\/10.3390\/horticulturae9040453","journal-title":"Horticulturae"},{"key":"5_CR38","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/978-1-4939-8594-4_4","volume-title":"Methods in molecular biology","author":"M Quambusch","year":"2018","unstructured":"Quambusch M, Winkelmann T (2018) Bacterial endophytes in plant tissue culture: mode of action, detection, and control. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Methods in molecular biology. Humana Press, New York, pp 69\u201388. https:\/\/doi.org\/10.1007\/978-1-4939-8594-4_4"},{"key":"5_CR39","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1007\/978-1-4612-3168-4_9","volume-title":"Microbial ecology of leaves","author":"O Petrini","year":"1991","unstructured":"Petrini O (1991) Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds) Microbial ecology of leaves. Springer, New York, pp 427\u2013428. https:\/\/doi.org\/10.1007\/978-1-4612-3168-4_9"},{"issue":"3","key":"5_CR40","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1007\/BF00398662","volume":"172","author":"EM Herman","year":"1987","unstructured":"Herman EM (1987) Immunogold-localization and synthesis of an oil-body membrane protein in developing soybean seeds. Planta 172(3):336\u2013345. https:\/\/doi.org\/10.1007\/BF00398662","journal-title":"Planta"},{"key":"5_CR41","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1111\/j.1365-2672.1989.tb02505.x","volume":"67","author":"C Leifert","year":"1989","unstructured":"Leifert C, Waites WM, Nicholas JR (1989) Bacterial contaminants of micropropagated plant cultures. J Appl Bacteriol 67:353\u2013361. https:\/\/doi.org\/10.1111\/j.1365-2672.1989.tb02505.x","journal-title":"J Appl Bacteriol"},{"key":"5_CR42","doi-asserted-by":"publisher","first-page":"546","DOI":"10.1079\/IVP2005673","volume":"41","author":"THA Liu","year":"2005","unstructured":"Liu THA, Hsu NW, Wu RY (2005) Control of leaf-tip necrosis of micropropagated ornamental statice by elimination of endophytic bacteria. In Vitro Cell Dev Biol Plant 41:546\u2013549. https:\/\/doi.org\/10.1079\/IVP2005673","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR43","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1007\/s11627-019-09986-2","volume":"55","author":"GF Leone","year":"2019","unstructured":"Leone GF, Andrade PAM, Almeida CV et al (2019) Use of antibiotics to control endophytic bacterial growth migration onto culture medium in Eucalyptus cloeziana F. Muell.: a micropropagation approach. In Vitro Cell Dev Biol Plant 55:421\u2013432. https:\/\/doi.org\/10.1007\/s11627-019-09986-2","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR44","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1007\/BF02632060","volume":"27","author":"PR Viss","year":"1991","unstructured":"Viss PR, Brooks EM, Driver JA (1991) A simplified method for the control of bacterial contamination in woody plant tissue culture. In Vitro Cell Dev Biol Plant 27:42\u201342. https:\/\/doi.org\/10.1007\/BF02632060","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR45","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1111\/j.1399-3054.1962.tb08052.x","volume":"15","author":"T Murashige","year":"1962","unstructured":"Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473\u2013497. https:\/\/doi.org\/10.1111\/j.1399-3054.1962.tb08052.x","journal-title":"Physiol Plant"},{"key":"5_CR46","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/s11240-008-9340-x","volume":"93","author":"P Thomas","year":"2008","unstructured":"Thomas P, Swarna GK, Patil P et al (2008) Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue cultures. Plant Cell Tissue Organ Cult 93:39\u201354. https:\/\/doi.org\/10.1007\/s11240-008-9340-x","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR47","doi-asserted-by":"publisher","first-page":"99","DOI":"10.3329\/ptcb.v28i1.37202","volume":"28","author":"M Ali","year":"2018","unstructured":"Ali M, Boonerjee S, Islam MN et al (2018) Endogenous bacterial contamination of plant tissue culture materials: identification and control strategy. Plant Tissue Cult Biotechnol 28:99\u2013108. https:\/\/doi.org\/10.3329\/ptcb.v28i1.37202","journal-title":"Plant Tissue Cult Biotechnol"},{"key":"5_CR48","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/BF00037716","volume":"36","author":"C Barrett","year":"1994","unstructured":"Barrett C, Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium \u00d7 domesticum cv. \u201cGrand Slam\u201d explants in vitro. Plant Cell Tissue Organ Cult 36:169\u2013175","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR49","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/BF00033619","volume":"29","author":"W Kneifel","year":"1992","unstructured":"Kneifel W, Leonhardt W (1992) Testing of different antibiotics against Gram-positive and Gram-negative bacteria isolated from plant tissue culture. Plant Cell Tissue Organ Cult 29:139\u2013144. https:\/\/doi.org\/10.1007\/BF00033619","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR50","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1007\/s10658-014-0559-6","volume":"141","author":"BG Marino","year":"2015","unstructured":"Marino BG, Gagg\u00eca F, Baffoni L et al (2015) Antimicrobial activity of Melia azedarach fruit extracts for control of bacteria in inoculated in-vitro shoots of \u2018MRS 2\/5\u2019 plum hybrid and calla lily and extract influence on the shoot cultures. Eur J Plant Pathol 141:505\u2013521. https:\/\/doi.org\/10.1007\/s10658-014-0559-6","journal-title":"Eur J Plant Pathol"},{"key":"5_CR51","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/s11240-019-01570-1","volume":"137","author":"R Meziani","year":"2019","unstructured":"Meziani R, Mazri MA, Essarioui A et al (2019) Towards a new approach of controlling endophytic bacteria associated with date palm explants using essential oils, aqueous and methanolic extracts from medicinal and aromatic plants. Plant Cell Tissue Organ Cult 137:285\u2013295. https:\/\/doi.org\/10.1007\/s11240-019-01570-1","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR52","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1007\/s11240-022-02239-y","volume":"149","author":"N Ahmed","year":"2022","unstructured":"Ahmed N, Mohamed HF, Xu C et al (2022) A novel surface sterilization method using Artemisia dracunculus extract for tissue culturing of endangered species Sargassum fusiforme. Plant Cell Tissue Organ Cult 149:135\u2013145. https:\/\/doi.org\/10.1007\/s11240-022-02239-y","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR53","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1007\/s00284-008-9122-z","volume":"56","author":"G Huys","year":"2008","unstructured":"Huys G, Vanhoutte T, Joossens M et al (2008) Coamplification of eukaryotic DNA with 16S rRNA gene-based PCR primers: possible consequences for population fingerprinting of complex microbial communities. Curr Microbiol 56:553\u2013557. https:\/\/doi.org\/10.1007\/s00284-008-9122-z","journal-title":"Curr Microbiol"},{"key":"5_CR54","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1007\/s11240-006-9087-1","volume":"85","author":"H Lata","year":"2006","unstructured":"Lata H, Li XC, Silva B et al (2006) Identification of IAA-producing endophytic bacteria from micropropagated Echinacea plants using 16S rRNA sequencing. Plant Cell Tissue Organ Cult 85:353\u2013359. https:\/\/doi.org\/10.1007\/s11240-006-9087-1","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR55","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1007\/s11240-008-9408-7","volume":"95","author":"C Luna","year":"2008","unstructured":"Luna C, Collavino M, Mroginski L et al (2008) Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis. Plant Cell Tissue Organ Cult 95:13\u201319. https:\/\/doi.org\/10.1007\/s11240-008-9408-7","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR56","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1007\/s11627-013-9500-5","volume":"49","author":"C Luna","year":"2013","unstructured":"Luna C, Acevedo R, Collavino M et al (2013) Endophytic bacteria from Ilex paraguariensis shoot cultures: localization, characterization, and response to isothiazolone biocides. In Vitro Cell Dev Biol Plant 49:326\u2013332. https:\/\/doi.org\/10.1007\/s11627-013-9500-5","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR57","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1007\/s11240-014-0553-x","volume":"119","author":"S Moreno-V\u00e1zquez","year":"2014","unstructured":"Moreno-V\u00e1zquez S, Larra\u00f1aga N, Uberhuaga EC et al (2014) Bacterial contamination of in vitro plant cultures: confounding effects on somaclonal variation and detection of contamination in plant tissues. Plant Cell Tissue Organ Cult 119:533\u2013541. https:\/\/doi.org\/10.1007\/s11240-014-0553-x","journal-title":"Plant Cell Tissue Organ Cult"},{"key":"5_CR58","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1007\/s11627-020-10125-5","volume":"57","author":"V Srivastava","year":"2021","unstructured":"Srivastava V, Nerwal DK, Kandan A et al (2021) Management of microbial contaminants in the In Vitro Gene Bank: a case study of taro [Colocasia esculenta (L.) Schott]. In Vitro Cell Dev Biol Plant 57:152\u2013163. https:\/\/doi.org\/10.1007\/s11627-020-10125-5","journal-title":"In Vitro Cell Dev Biol Plant"},{"key":"5_CR59","doi-asserted-by":"publisher","first-page":"903","DOI":"10.3389\/fpls.2020.00903","volume":"11","author":"ML Izarra","year":"2020","unstructured":"Izarra ML, Panta AL, Maza CR et al (2020) Identification and control of latent bacteria in in vitro cultures of sweetpotato [Ipomoea batatas (L.) Lam]. Front Plant Sci 11:903. https:\/\/doi.org\/10.3389\/fpls.2020.00903","journal-title":"Front Plant Sci"},{"key":"5_CR60","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.sajb.2021.01.024","volume":"139","author":"MK Seliem","year":"2021","unstructured":"Seliem MK, El-Mahrouk ME, El-Banna AN et al (2021) Micropropagation of Philodendron selloum: influence of copper sulfate on endophytic bacterial contamination, antioxidant enzyme activity, electrolyte leakage, and plant survival. S Afr J Bot 139:230\u2013240. https:\/\/doi.org\/10.1016\/j.sajb.2021.01.024","journal-title":"S Afr J Bot"},{"issue":"7","key":"5_CR61","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1094\/PHYTO-07-18-0216-R","volume":"109","author":"D Caldwell","year":"2019","unstructured":"Caldwell D, Iyer-Pascuzzi AS (2019) A scanning electron microscopy technique for viewing plant microbe interactions at tissue and cell-type resolution. Phytopathology 109(7):1302\u20131311. https:\/\/doi.org\/10.1094\/PHYTO-07-18-0216-R","journal-title":"Phytopathology"},{"key":"5_CR62","series-title":"Springer protocols handbooks","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/978-1-0716-2827-0_8","volume-title":"Endophytic microbes: isolation, identification, and bioactive potentials","author":"P Kumar","year":"2023","unstructured":"Kumar P, Rani S, Sarita et al (2023) Detection of endophytes by electron microscope. In: Sankaranarayanan A, Amaresan N, Dwivedi MK (eds) Endophytic microbes: isolation, identification, and bioactive potentials, Springer protocols handbooks. Humana Press, New York, pp 71\u201376. https:\/\/doi.org\/10.1007\/978-1-0716-2827-0_8"},{"issue":"10","key":"5_CR63","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1007\/s11274-021-03127-x","volume":"37","author":"GMF Bettencourt","year":"2021","unstructured":"Bettencourt GMF, Degenhardt J, Santos GD et al (2021) Metagenomic analyses, isolation and characterization of endophytic bacteria associated with Eucalyptus urophylla BRS07-01 in vitro plants. World J Microbiol Biotechnol 37(10):164. https:\/\/doi.org\/10.1007\/s11274-021-03127-x","journal-title":"World J Microbiol Biotechnol"},{"key":"5_CR64","first-page":"11","volume-title":"T\u00e9cnicas de Microscopia Eletr\u00f4nica Aplicada \u00e0s Ci\u00eancias Biol\u00f3gicas","author":"A Sesso","year":"2007","unstructured":"Sesso A (2007) Fixa\u00e7\u00e3o de sistemas biol\u00f3gico. In: Souza W (ed) T\u00e9cnicas de Microscopia Eletr\u00f4nica Aplicada \u00e0s Ci\u00eancias Biol\u00f3gicas. Sociedade Brasileira de Microscopia, Rio de Janeiro, pp 11\u201330"}],"container-title":["Methods in Molecular Biology","Plant Cell Culture Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-3954-2_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T13:03:36Z","timestamp":1720616616000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-3954-2_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071639535","9781071639542"],"references-count":64,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-3954-2_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"11 July 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}