{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T01:22:14Z","timestamp":1771636934410,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":13,"publisher":"Springer US","isbn-type":[{"value":"9781071640265","type":"print"},{"value":"9781071640272","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024]]},"DOI":"10.1007\/978-1-0716-4027-2_12","type":"book-chapter","created":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T16:21:59Z","timestamp":1721751719000},"page":"137-148","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue"],"prefix":"10.1007","author":[{"given":"Xiaonan","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanqing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghong","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,24]]},"reference":[{"issue":"2","key":"12_CR1","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1073\/pnas.63.2.378","volume":"63","author":"JG Gall","year":"1969","unstructured":"Gall JG, Pardue ML (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63(2):378\u2013383. https:\/\/doi.org\/10.1073\/pnas.63.2.378","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5206","key":"12_CR2","doi-asserted-by":"publisher","first-page":"582","DOI":"10.1038\/223582a0","volume":"223","author":"HA John","year":"1969","unstructured":"John HA, Birnstiel ML, Jones KW (1969) RNA-DNA hybrids at the cytological level. Nature 223(5206):582\u2013587. https:\/\/doi.org\/10.1038\/223582a0","journal-title":"Nature"},{"issue":"1","key":"12_CR3","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1002\/jmv.1890080106","volume":"8","author":"EJ Gowans","year":"1981","unstructured":"Gowans EJ, Burrell CJ, Jilbert AR, Marmion BP (1981) Detection of hepatitis B virus DNA sequences in infected hepatocytes by in situ cytohybridisation. J Med Virol 8(1):67\u201378","journal-title":"J Med Virol"},{"issue":"2","key":"12_CR4","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1016\/0014-4827(80)90087-7","volume":"128","author":"JG Bauman","year":"1980","unstructured":"Bauman JG, Wiegant J, Borst P, van Duijn P (1980) A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA. Exp Cell Res 128(2):485\u2013490. https:\/\/doi.org\/10.1016\/0014-4827(80)90087-7","journal-title":"Exp Cell Res"},{"issue":"9","key":"12_CR5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075120","volume":"8","author":"SM Shaffer","year":"2013","unstructured":"Shaffer SM, Wu MT, Levesque MJ, Raj A (2013) Turbo FISH: a method for rapid single molecule RNA FISH. PLoS One 8(9):e75120. https:\/\/doi.org\/10.1371\/journal.pone.0075120","journal-title":"PLoS One"},{"issue":"11","key":"12_CR6","doi-asserted-by":"publisher","first-page":"1208","DOI":"10.1038\/nbt.1692","volume":"28","author":"HM Choi","year":"2010","unstructured":"Choi HM, Chang JY, Trinh le A, Padilla JE, Fraser SE, Pierce NA (2010) Programmable in situ amplification for multiplexed imaging of mRNA expression. Nat Biotechnol 28(11):1208\u20131212. https:\/\/doi.org\/10.1038\/nbt.1692","journal-title":"Nat Biotechnol"},{"issue":"11","key":"12_CR7","doi-asserted-by":"publisher","first-page":"6633","DOI":"10.1073\/pnas.78.11.6633","volume":"78","author":"PR Langer","year":"1981","unstructured":"Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78(11):6633\u20136637. https:\/\/doi.org\/10.1073\/pnas.78.11.6633","journal-title":"Proc Natl Acad Sci USA"},{"issue":"7","key":"12_CR8","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1136\/jcp.41.7.793","volume":"41","author":"NV Naoumov","year":"1988","unstructured":"Naoumov NV, Alexander GJ, Eddleston AL, Williams R (1988) In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes. J Clin Pathol 41(7):793\u2013798. https:\/\/doi.org\/10.1136\/jcp.41.7.793","journal-title":"J Clin Pathol"},{"issue":"19","key":"12_CR9","doi-asserted-by":"publisher","first-page":"5843","DOI":"10.1093\/nar\/18.19.5843","volume":"18","author":"HJ Holtke","year":"1990","unstructured":"Holtke HJ, Kessler C (1990) Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection. Nucleic Acids Res 18(19):5843\u20135851. https:\/\/doi.org\/10.1093\/nar\/18.19.5843","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"12_CR10","doi-asserted-by":"publisher","first-page":"3196","DOI":"10.1128\/jcm.34.12.3196-3202.1996","volume":"34","author":"D Kern","year":"1996","unstructured":"Kern D, Collins M, Fultz T, Detmer J, Hamren S, Peterkin JJ, Sheridan P, Urdea M, White R, Yeghiazarian T, Todd J (1996) An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma. J Clin Microbiol 34(12):3196\u20133202. https:\/\/doi.org\/10.1128\/jcm.34.12.3196-3202.1996","journal-title":"J Clin Microbiol"},{"issue":"3","key":"12_CR11","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1309\/90BU6KDXANFLN4RJ","volume":"126","author":"GJ Tsongalis","year":"2006","unstructured":"Tsongalis GJ (2006) Branched DNA technology in molecular diagnostics. Am J Clin Pathol 126(3):448\u2013453. https:\/\/doi.org\/10.1309\/90BU6KDXANFLN4RJ","journal-title":"Am J Clin Pathol"},{"issue":"5","key":"12_CR12","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1177\/002215540104900507","volume":"49","author":"AN Player","year":"2001","unstructured":"Player AN, Shen LP, Kenny D, Antao VP, Kolberg JA (2001) Single-copy gene detection using branched DNA (bDNA) in situ hybridization. J Histochem Cytochem 49(5):603\u2013611. https:\/\/doi.org\/10.1177\/002215540104900507","journal-title":"J Histochem Cytochem"},{"issue":"3","key":"12_CR13","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1172\/jci83339","volume":"126","author":"X Zhang","year":"2016","unstructured":"Zhang X, Lu W, Zheng Y, Wang W, Bai L, Chen L, Feng Y, Zhang Z, Yuan Z (2016) In situ analysis of intrahepatic virological events in chronic hepatitis B virus infection. J Clin Invest 126(3):1079\u20131092. https:\/\/doi.org\/10.1172\/jci83339","journal-title":"J Clin Invest"}],"container-title":["Methods in Molecular Biology","Hepatitis B Virus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4027-2_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,23]],"date-time":"2024-07-23T16:22:35Z","timestamp":1721751755000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4027-2_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"ISBN":["9781071640265","9781071640272"],"references-count":13,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4027-2_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024]]},"assertion":[{"value":"24 July 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}