{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T14:42:39Z","timestamp":1742913759133,"version":"3.40.3"},"publisher-location":"New York, NY","reference-count":16,"publisher":"Springer US","isbn-type":[{"type":"print","value":"9781071641033"},{"type":"electronic","value":"9781071641040"}],"license":[{"start":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T00:00:00Z","timestamp":1729987200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,10,27]],"date-time":"2024-10-27T00:00:00Z","timestamp":1729987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4104-0_2","type":"book-chapter","created":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T09:02:33Z","timestamp":1729933353000},"page":"7-16","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Large-Scale Transient Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the Production of the Trimeric SARS-CoV-2 Spike Protein"],"prefix":"10.1007","author":[{"given":"David L.","family":"Hacker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelvin","family":"Lau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Durrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya","family":"Quinche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Fran\u00e7ois","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ludovic","family":"Delisle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Pojer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,10,27]]},"reference":[{"key":"2_CR1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn423","volume":"36","author":"G Backliwal","year":"2008","unstructured":"Backliwal G, Hildinger M, Chenuet S, Wulhfard S, De Jesus M, Wurm FM (2008) Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g\/l by transient transfection under serum-free conditions. Nucleic Acids Res 36:e96","journal-title":"Nucleic Acids Res"},{"key":"2_CR2","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.jbiotec.2011.03.001","volume":"153","author":"Y Rajendra","year":"2011","unstructured":"Rajendra Y, Kiseljak D, Baldi L, Hacker DL, Wurm FM (2011) A simple high-yielding process for transient gene expression in CHO cells. J Biotechnol 153:22\u201326","journal-title":"J Biotechnol"},{"key":"2_CR3","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.1007\/s10529-015-1938-6","volume":"37","author":"Y Rajendra","year":"2015","unstructured":"Rajendra Y, Hougland MD, Schmitt MG, Barnard GC (2015) Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells. Biotechnol Lett 37:2379\u20132386","journal-title":"Biotechnol Lett"},{"key":"2_CR4","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1002\/btpr.2414","volume":"33","author":"Y Rajendra","year":"2017","unstructured":"Rajendra Y, Peery RB, Hougland MD, Barnard GC, Wu X, Fitchett JR, Bacica M, Demarest SJ (2017) Transient and stable CHO gene expression, purification and characterization of novel hetero-dimeric bispecific IgG antibodies. Biotechnol Prog 33:469\u2013477","journal-title":"Biotechnol Prog"},{"key":"2_CR5","doi-asserted-by":"publisher","first-page":"e01828","DOI":"10.1128\/JVI.01828-20","volume":"95","author":"C Fenwick","year":"2021","unstructured":"Fenwick C, Croxatto A, Coste AT, Pojer F, Andr\u00e9 C, Pellaton C, Farina A, Campos J, Hacker D, Lau K, Bosch BJ, Gonseth Nussle S, Bochud M, D\u2019Acremont V, Trono D, Greub G, Pantaleo G (2021) Changes in SARS-CoV-2 spike versus nucleocapsid antibody responses impact the estimates of infections in population-based seroprevalence studies. J Virol 95:e01828\u2013e01820","journal-title":"J Virol"},{"key":"2_CR6","doi-asserted-by":"publisher","first-page":"eabi8452","DOI":"10.1126\/scitranslmed.abi8452","volume":"13","author":"C Fenwick","year":"2021","unstructured":"Fenwick C, Turelli P, Pellaton C, Farina A, Campos J, Raclot C, Pojer F, Cagno V, Nussl\u00e9 SG, D\u2019Acremont V, Fehr J, Puhan M, Pantaleo G, Trono D (2021) A high-throughput cell- and virus-free assay shows reduced neutralization of SARS-CoV-2 variants by COVID convalescent plasma. Sci Transl Med 13:eabi8452","journal-title":"Sci Transl Med"},{"key":"2_CR7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2025289118","volume":"118","author":"Z Swank","year":"2021","unstructured":"Swank Z, Michielin G, Yip HM, Cohen P, Andrey DO, Vuilleumier N, Kaiser L, Eckerle I, Meyer B, Maerkl SJ (2021) A high-throughput microfluidic nanoassay for detecting anti-SARS-CoV-2 antibodies in serum or ultra-low volume samples. Proc Natl Acad Sci USA 118:e2025289118","journal-title":"Proc Natl Acad Sci USA"},{"key":"2_CR8","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109814","volume":"37","author":"C Fenwick","year":"2021","unstructured":"Fenwick C, Turelli P, Perez L, Pellaton C, Esteves-Leuenberger L, Farina A, Campos J, Lana E, Fiscalini F, Raclot C, Pojer F, Lau K, Demurtas D, Descatoire M, Joo VS, Foglierini M, Noto A, Abdelnabi R, Foo CS, Vangeel L, Neyts J, Du W, Bosch BJ, Veldman G, Leyssen P, Thiel V, LeGrand R, L\u00e9vy Y, Trono D, Pantaleo G (2021) A highly potent antibody effective against SARS-CoV-2 variants of concern. Cell Rep 37:109814","journal-title":"Cell Rep"},{"key":"2_CR9","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1038\/s41564-022-01198-6","volume":"7","author":"C Fenwick","year":"2022","unstructured":"Fenwick C, Turelli P, Ni D, Perez L, Lau K, Herate C, Marlin R, Lana E, Pellaton C, Raclot C, Esteves-Leuenberger L, Campos J, Farina A, Fiscalini F, Dereuddre-Bosquet N, Relouzat F, Abdelnabi R, Foo CS, Neyts J, Leyssen P, L\u00e9vy Y, Pojer F, Stahlberg H, LeGrand R, Trono D, Pantaleo G (2022) Patient-derived monoclonal antibody neutralizes SARS-CoV-2 Omicron variants and confers full protection in monkeys. Nat Microbiol 7:1376\u20131389","journal-title":"Nat Microbiol"},{"key":"2_CR10","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1126\/science.abb2507","volume":"367","author":"D Wrapp","year":"2020","unstructured":"Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh CL, Abiona O, Graham BS, McLellan JS (2020) Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 367:1260\u20131263","journal-title":"Science"},{"key":"2_CR11","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1002\/bit.20358","volume":"89","author":"N Muller","year":"2005","unstructured":"Muller N, Girard P, Hacker DL, Jordan M, Wurm FM (2005) Orbital shaker technology for the cultivation of mammalian cells in suspension. Biotechnol Bioeng 89:400\u2013406","journal-title":"Biotechnol Bioeng"},{"key":"2_CR12","first-page":"45","volume":"138","author":"W Klockner","year":"2014","unstructured":"Klockner W, Diederichs S, Buchs J (2014) Orbitally shaken single-use bioreactors. Adv Biochem Eng Biotechnol 138:45\u201360","journal-title":"Adv Biochem Eng Biotechnol"},{"key":"2_CR13","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.bej.2013.04.008","volume":"76","author":"DT Monteil","year":"2013","unstructured":"Monteil DT, Tontodonati G, Ghimire S, Baldi L, Hacker DL, B\u00fcrki CA, Wurm FM (2013) Disposable 600-mL orbitally shaken bioreactor for mammalian cell cultivation in suspension. Biochem Eng J 76:6\u201312","journal-title":"Biochem Eng J"},{"key":"2_CR14","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1007\/978-1-4939-8730-6_9","volume":"1850","author":"DL Hacker","year":"2018","unstructured":"Hacker DL, Durrer L, Quinche S (2018) CHO and HEK293 cultivation and transfection in single-use orbitally shaken bioreactors. Methods Mol Biol 1850:123\u2013131","journal-title":"Methods Mol Biol"},{"key":"2_CR15","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1007\/978-1-4939-6972-2_3","volume":"1603","author":"Y Rajendra","year":"2017","unstructured":"Rajendra Y, Balasubramanian S, Hacker DL (2017) Large-scale transient transfection of Chinese hamster ovary cells in suspension. Methods Mol Biol 1603:45\u201355","journal-title":"Methods Mol Biol"},{"key":"2_CR16","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1002\/btpr.2152","volume":"31","author":"Y Rajendra","year":"2015","unstructured":"Rajendra Y, Balasubramanian S, Kiseljak D, Baldi L, Wurm FM, Hacker DL (2015) Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents. Biotechnol Prog 31:1571\u20131578","journal-title":"Biotechnol Prog"}],"container-title":["Methods in Molecular Biology","Heterologous Protein Production in CHO Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4104-0_2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T09:06:36Z","timestamp":1729933596000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4104-0_2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,27]]},"ISBN":["9781071641033","9781071641040"],"references-count":16,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4104-0_2","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"type":"print","value":"1064-3745"},{"type":"electronic","value":"1940-6029"}],"subject":[],"published":{"date-parts":[[2024,10,27]]},"assertion":[{"value":"27 October 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}