{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:42:12Z","timestamp":1784875332150,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":10,"publisher":"Springer US","isbn-type":[{"value":"9781071642559","type":"print"},{"value":"9781071642566","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4256-6_27","type":"book-chapter","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T15:08:28Z","timestamp":1732547308000},"page":"387-405","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Droplet-Based Single-Cell 3\u2032 mRNA Sequencing of Marburg Virus-Infected Samples"],"prefix":"10.1007","author":[{"given":"Michelle","family":"Meyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Palaniappan","family":"Ramanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,26]]},"reference":[{"key":"27_CR1","unstructured":"Genomics X (2019) Chromium Next GEM Single Cell 3\u2032 Reagent Kits v3.1 user guide. https:\/\/cdn.10xgenomics.com\/image\/upload\/v1660261285\/support-documents\/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf"},{"key":"27_CR2","doi-asserted-by":"publisher","first-page":"14049","DOI":"10.1038\/ncomms14049","volume":"8","author":"GX Zheng","year":"2017","unstructured":"Zheng GX, Terry JM, Belgrader P, Ryvkin P, Bent ZW, Wilson R, Ziraldo SB, Wheeler TD, McDermott GP, Zhu J, Gregory MT, Shuga J, Montesclaros L, Underwood JG, Masquelier DA, Nishimura SY, Schnall-Levin M, Wyatt PW, Hindson CM, Bharadwaj R, Wong A, Ness KD, Beppu LW, Deeg HJ, McFarland C, Loeb KR, Valente WJ, Ericson NG, Stevens EA, Radich JP, Mikkelsen TS, Hindson BJ, Bielas JH (2017) Massively parallel digital transcriptional profiling of single cells. Nat Commun 8:14049. https:\/\/doi.org\/10.1038\/ncomms14049","journal-title":"Nat Commun"},{"key":"27_CR3","doi-asserted-by":"publisher","first-page":"34589","DOI":"10.1038\/srep34589","volume":"6","author":"M Holzer","year":"2016","unstructured":"Holzer M, Krahling V, Amman F, Barth E, Bernhart SH, Carmelo VA, Collatz M, Doose G, Eggenhofer F, Ewald J, Fallmann J, Feldhahn LM, Fricke M, Gebauer J, Gruber AJ, Hufsky F, Indrischek H, Kanton S, Linde J, Mostajo N, Ochsenreiter R, Riege K, Rivarola-Duarte L, Sahyoun AH, Saunders SJ, Seemann SE, Tanzer A, Vogel B, Wehner S, Wolfinger MT, Backofen R, Gorodkin J, Grosse I, Hofacker I, Hoffmann S, Kaleta C, Stadler PF, Becker S, Marz M (2016) Differential transcriptional responses to Ebola and Marburg virus infection in bat and human cells. Sci Rep 6:34589. https:\/\/doi.org\/10.1038\/srep34589","journal-title":"Sci Rep"},{"key":"27_CR4","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1186\/s12864-016-3060-0","volume":"17","author":"IS Caballero","year":"2016","unstructured":"Caballero IS, Honko AN, Gire SK, Winnicki SM, Mele M, Gerhardinger C, Lin AE, Rinn JL, Sabeti PC, Hensley LE, Connor JH (2016) In vivo Ebola virus infection leads to a strong innate response in circulating immune cells. BMC Genomics 17:707. https:\/\/doi.org\/10.1186\/s12864-016-3060-0","journal-title":"BMC Genomics"},{"issue":"1","key":"27_CR5","doi-asserted-by":"publisher","first-page":"3866","DOI":"10.1038\/s41467-023-39627-7","volume":"14","author":"L Santus","year":"2023","unstructured":"Santus L, Sopena-Rios M, Garcia-Perez R, Lin AE, Adams GC, Barnes KG, Siddle KJ, Wohl S, Reverter F, Rinn JL, Bennett RS, Hensley LE, Sabeti PC, Mele M (2023) Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques. Nat Commun 14(1):3866. https:\/\/doi.org\/10.1038\/s41467-023-39627-7","journal-title":"Nat Commun"},{"issue":"5","key":"27_CR6","doi-asserted-by":"publisher","first-page":"1383","DOI":"10.1016\/j.cell.2020.10.002","volume":"183","author":"D Kotliar","year":"2020","unstructured":"Kotliar D, Lin AE, Logue J, Hughes TK, Khoury NM, Raju SS, Wadsworth MH 2nd, Chen H, Kurtz JR, Dighero-Kemp B, Bjornson ZB, Mukherjee N, Sellers BA, Tran N, Bauer MR, Adams GC, Adams R, Rinn JL, Mele M, Schaffner SF, Nolan GP, Barnes KG, Hensley LE, McIlwain DR, Shalek AK, Sabeti PC, Bennett RS (2020) Single-cell profiling of Ebola virus disease in vivo reveals viral and host dynamics. Cell 183(5):1383\u20131401.e1319. https:\/\/doi.org\/10.1016\/j.cell.2020.10.002","journal-title":"Cell"},{"key":"27_CR7","unstructured":"Illumina Pooling Calculator. https:\/\/support.illumina.com\/help\/pooling-calculator\/pooling-calculator.htm"},{"key":"27_CR8","unstructured":"Genomics X (2021) Isolation of leukocytes, bone marrow and peripheral blood mononuclear cells for single cell RNA sequencing. https:\/\/cdn.10xgenomics.com\/image\/upload\/v1660261285\/support-documents\/CG000392_Demonstrated_Protocol_Isolation_of-PBMC_SingleCellRNA_Sequencing_Rev_A.pdf"},{"key":"27_CR9","unstructured":"Genomics X (2022) Chromium controller readiness test. https:\/\/cdn.10xgenomics.com\/image\/upload\/v1666737555\/support-documents\/CG000222_ControllerReadinessTestUserGuideNextGEM__RevF__1_.pdf"},{"key":"27_CR10","unstructured":"Genomics X (2022) Best practices to minimize chromium next GEM chip clogs and wetting failures. https:\/\/cdn.10xgenomics.com\/image\/upload\/v1660261285\/support-documents\/CG000479_TechNote_MinimizingClogs_WettingFailures_RevB.pdf"}],"container-title":["Methods in Molecular Biology","Marburg Virus"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4256-6_27","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T16:05:27Z","timestamp":1732550727000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4256-6_27"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,26]]},"ISBN":["9781071642559","9781071642566"],"references-count":10,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4256-6_27","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,26]]},"assertion":[{"value":"26 November 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}