{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T16:18:30Z","timestamp":1781540310218,"version":"3.54.5"},"publisher-location":"New York, NY","reference-count":32,"publisher":"Springer US","isbn-type":[{"value":"9781071643297","type":"print"},{"value":"9781071643303","type":"electronic"}],"license":[{"start":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T00:00:00Z","timestamp":1735344000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T00:00:00Z","timestamp":1735344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4330-3_3","type":"book-chapter","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T14:28:26Z","timestamp":1735309706000},"page":"35-47","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Protoplast Transformation of Phytophthora spp."],"prefix":"10.1007","author":[{"given":"Lydia R. J.","family":"Welsh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C.","family":"Whisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,12,28]]},"reference":[{"key":"3_CR1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1111\/mpp.12190","volume":"16","author":"S Kamoun","year":"2015","unstructured":"Kamoun S, Furzer O, Jones JD, Judelson HS, Ali GS, Dalio RJ, Roy SG, Schena L, Zambounis A, Panabi\u00e8res F, Cahill D, Ruocco M, Figueiredo A, Chen XR, Hulvey J, Stam R, Lamour K, Gijzen M, Tyler BM, Gr\u00fcnwald NJ, Mukhtar MS, Tom\u00e9 DF, T\u00f6r M, Van Den Ackerveken G, McDowell J, Daayf F, Fry WE, Lindqvist-Kreuze H, Meijer HJ, Petre B, Ristaino J, Yoshida K, Birch PR, Govers F (2015) The top 10 oomycete pathogens in molecular plant pathology. Mol Plant Pathol 16:413\u2013434","journal-title":"Mol Plant Pathol"},{"key":"3_CR2","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1007\/978-1-61737-998-7_11","volume":"712","author":"E Huitema","year":"2011","unstructured":"Huitema E, Smoker M, Kamoun S (2011) A straightforward protocol for electro-transformation of Phytophthora capsici zoospores. Methods Mol Biol 712:129\u2013135","journal-title":"Methods Mol Biol"},{"key":"3_CR3","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1046\/j.1364-3703.2003.00191.x","volume":"4","author":"I Vijn","year":"2003","unstructured":"Vijn I, Govers F (2003) Agrobacterium tumefaciens mediated transformation of the oomycete plant pathogen Phytophthora infestans. Mol Plant Pathol 4:459\u2013467","journal-title":"Mol Plant Pathol"},{"key":"3_CR4","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1007\/s00294-002-0354-3","volume":"42","author":"C Cvitanich","year":"2003","unstructured":"Cvitanich C, Judelson HS (2003) Stable transformation of the oomycete, Phytophthora infestans, using microprojectile bombardment. Curr Genet 42:228\u2013235","journal-title":"Curr Genet"},{"key":"3_CR5","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1094\/MPMI-4-602","volume":"4","author":"HS Judelson","year":"1991","unstructured":"Judelson HS, Tyler BM, Michelmore RW (1991) Transformation of the oomycete pathogen, Phytophthora infestans. Mol Plant\u2013Microbe Interact 4:602\u2013607","journal-title":"Mol Plant\u2013Microbe Interact"},{"key":"3_CR6","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1016\/j.fgb.2008.05.009","volume":"45","author":"AMV Ah-Fong","year":"2008","unstructured":"Ah-Fong AMV, Bormann-Chung CA, Judelson HS (2008) Optimization of transgene-mediated silencing in Phytophthora infestans and its association with small-interfering RNAs. Fungal Genet Biol 45:1197\u20131205","journal-title":"Fungal Genet Biol"},{"key":"3_CR7","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1016\/j.funbio.2011.07.001","volume":"115","author":"AM Ah-Fong","year":"2011","unstructured":"Ah-Fong AM, Judelson HS (2011) Vectors for fluorescent protein tagging in Phytophthora: tools for functional genomics and cell biology. Fungal Biol 115:882\u2013890","journal-title":"Fungal Biol"},{"key":"3_CR8","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1007\/BF00351498","volume":"23","author":"HS Judelson","year":"1993","unstructured":"Judelson HS, Coffey MD, Arredondo FR, Tyler BM (1993) Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes. Curr Genet 23:211\u2013218","journal-title":"Curr Genet"},{"key":"3_CR9","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.4352","volume":"12","author":"J Cao","year":"2022","unstructured":"Cao J, Qiu M, Ye W, Wang Y (2022) Phytophthora sojae transformation based on the CRISPR\/Cas9 system. Bio Protoc 12:e4352","journal-title":"Bio Protoc"},{"key":"3_CR10","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/978-1-4939-8724-5_17","volume":"1848","author":"Z Wang","year":"2018","unstructured":"Wang Z, Tyler BM, Liu X (2018) Protocol of Phytophthora capsici transformation using the CRISPR-Cas9 system. Methods Mol Biol 1848:265\u2013274","journal-title":"Methods Mol Biol"},{"key":"3_CR11","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.682754","volume":"12","author":"T Dai","year":"2021","unstructured":"Dai T, Xu Y, Yang X, Jiao B, Qiu M, Xue J, Arredondo F, Tyler BM (2021) An improved transformation system for Phytophthora cinnamomi using green fluorescent protein. Front Microbiol 12:682754","journal-title":"Front Microbiol"},{"key":"3_CR12","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1111\/mpp.12303","volume":"17","author":"XR Chen","year":"2016","unstructured":"Chen XR, Li YP, Li QY, Xing YP, Liu BB, Tong YH, Xu JY (2016) SCR96, a small cysteine-rich secretory protein of Phytophthora cactorum, can trigger cell death in the Solanaceae and is important for pathogenicity and oxidative stress tolerance. Mol Plant Pathol 17:577\u2013587","journal-title":"Mol Plant Pathol"},{"key":"3_CR13","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1111\/j.1574-6968.1999.tb13761.x","volume":"178","author":"P van West","year":"1999","unstructured":"van West P, Reid B, Campbell TA, Sandrock RW, Fry WE, Kamoun S, Gow NA (1999) Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora. FEMS Microbiol Lett 178:71\u201380","journal-title":"FEMS Microbiol Lett"},{"key":"3_CR14","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1111\/j.1574-6968.1999.tb13641.x","volume":"176","author":"A Bottin","year":"1999","unstructured":"Bottin A, Larche L, Villalba F, Gaulin E, Esquerr\u00e9-Tugay\u00e9 MT, Rickauer M (1999) Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae. FEMS Microbiol Lett 176:51\u201356","journal-title":"FEMS Microbiol Lett"},{"key":"3_CR15","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1111\/j.1550-7408.2008.00376.x","volume":"56","author":"M Riedel","year":"2009","unstructured":"Riedel M, Calmin G, Belbahri L, Lefort F, G\u00f6tz M, Wagner S, Werres S (2009) Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora ramorum. J Eukaryot Microbiol 56:130\u2013135","journal-title":"J Eukaryot Microbiol"},{"key":"3_CR16","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1046\/j.1364-3703.2003.00168.x","volume":"4","author":"A Si-Ammour","year":"2003","unstructured":"Si-Ammour A, Mauch-Mani B, Mauch F (2003) Quantification of induced resistance against Phytophthora species expressing GFP as a vital marker: beta-aminobutyric acid but not BTH protects potato and Arabidopsis from infection. Mol Plant Pathol 4:237\u2013248","journal-title":"Mol Plant Pathol"},{"key":"3_CR17","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1111\/mpp.12905","volume":"21","author":"J Situ","year":"2020","unstructured":"Situ J, Jiang L, Fan X, Yang W, Li W, Xi P, Deng Y, Kong G, Jiang Z (2020) An RXLR effector PlAvh142 from Peronophythora litchii triggers plant cell death and contributes to virulence. Mol Plant Pathol 21:415\u2013428","journal-title":"Mol Plant Pathol"},{"key":"3_CR18","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1111\/mpp.13072","volume":"22","author":"S Wang","year":"2021","unstructured":"Wang S, Vetukuri RR, Kushwaha SK, Hedley PE, Morris J, Studholme DJ, Welsh LRJ, Boevink PC, Birch PRJ, Whisson SC (2021) Haustorium formation and a distinct biotrophic transcriptome characterize infection of Nicotiana benthamiana by the tree pathogen Phytophthora kernoviae. Mol Plant Pathol 22:954\u2013968","journal-title":"Mol Plant Pathol"},{"key":"3_CR19","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1038\/nature06203","volume":"450","author":"SC Whisson","year":"2007","unstructured":"Whisson SC, Boevink PC, Moleleki L, Avrova AO, Morales JG, Gilroy EM, Armstrong MR, Grouffaud S, van West P, Chapman S, Hein I, Toth IK, Pritchard L, Birch PRJ (2007) A translocation signal for delivery of oomycete effector proteins into host plant cells. Nature 450:115\u2013118","journal-title":"Nature"},{"key":"3_CR20","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1128\/EC.5.4.745-752.2006","volume":"5","author":"S Tani","year":"2006","unstructured":"Tani S, Judelson HS (2006) Activation of zoosporogenesis-specific genes in Phytophthora infestans involves a 7-nucleotide promoter motif and cold-induced membrane rigidity. Eukaryot Cell 5:745\u2013752","journal-title":"Eukaryot Cell"},{"key":"3_CR21","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1111\/j.1462-5822.2008.01206.x","volume":"10","author":"AO Avrova","year":"2008","unstructured":"Avrova AO, Boevink PC, Young V, Grenville-Briggs LJ, van West P, Birch PR, Whisson SC (2008) A novel Phytophthora infestans haustorium-specific membrane protein is required for infection of potato. Cell Microbiol 10:2271\u20132284","journal-title":"Cell Microbiol"},{"key":"3_CR22","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1111\/mpp.12318","volume":"17","author":"Y Fang","year":"2016","unstructured":"Fang Y, Tyler BM (2016) Efficient disruption and replacement of an effector gene in the oomycete Phytophthora sojae using CRISPR\/Cas9. Mol Plant Pathol 17:127\u2013139","journal-title":"Mol Plant Pathol"},{"key":"3_CR23","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1007\/978-1-4939-8724-5_10","volume":"1848","author":"AMV Ah-Fong","year":"2018","unstructured":"Ah-Fong AMV, Kagda M, Judelson HS (2018) Illuminating Phytophthora biology with fluorescent protein tags. Methods Mol Biol 1848:119\u2013129","journal-title":"Methods Mol Biol"},{"key":"3_CR24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008138","volume":"16","author":"M Qiu","year":"2020","unstructured":"Qiu M, Li Y, Zhang X, Xuan M, Zhang B, Ye W, Zheng X, Govers F, Wang Y (2020) G protein \u03b1 subunit suppresses sporangium formation through a serine\/threonine protein kinase in Phytophthora sojae. PLoS Pathog 16:e1008138","journal-title":"PLoS Pathog"},{"key":"3_CR25","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1111\/mmi.14114","volume":"110","author":"W Leesutthiphonchai","year":"2018","unstructured":"Leesutthiphonchai W, Judelson HS (2018) A MADS-box transcription factor regulates a central step in sporulation of the oomycete Phytophthora infestans. Mol Microbiol 110:562\u2013575","journal-title":"Mol Microbiol"},{"key":"3_CR26","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1007\/BF00272355","volume":"234","author":"HS Judelson","year":"1992","unstructured":"Judelson HS, Tyler BM, Michelmore RW (1992) Regulatory sequences for expressing genes in oomycete fungi. Mol Gen Genet 234:138\u2013146","journal-title":"Mol Gen Genet"},{"key":"3_CR27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145612","volume":"10","author":"L Poidevin","year":"2015","unstructured":"Poidevin L, Andreeva K, Khachatoorian C, Judelson HS (2015) Comparisons of ribosomal protein gene promoters indicate superiority of heterologous regulatory sequences for expressing transgenes in Phytophthora infestans. PLoS One 10:e0145612","journal-title":"PLoS One"},{"key":"3_CR28","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.fgb.2009.02.006","volume":"46","author":"Q Xiang","year":"2009","unstructured":"Xiang Q, Kim KS, Roy S, Judelson HS (2009) A motif within a complex promoter from the oomycete Phytophthora infestans determines transcription during an intermediate stage of sporulation. Fungal Genet Biol 46:400\u2013409","journal-title":"Fungal Genet Biol"},{"key":"3_CR29","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1111\/mpp.12161","volume":"16","author":"H Gamboa-Mel\u00e9ndez","year":"2015","unstructured":"Gamboa-Mel\u00e9ndez H, Judelson HS (2015) Development of a bipartite ecdysone-responsive gene switch for the oomycete Phytophthora infestans and its use to manipulate transcription during axenic culture and plant infection. Mol Plant Pathol 16:83\u201391","journal-title":"Mol Plant Pathol"},{"key":"3_CR30","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/BF00281624","volume":"239","author":"HS Judelson","year":"1993","unstructured":"Judelson HS (1993) Intermolecular ligation mediates efficient cotransformation in Phytophthora infestans. Mol Gen Genet 239:241\u2013250","journal-title":"Mol Gen Genet"},{"key":"3_CR31","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1186\/s12866-019-1642-0","volume":"19","author":"E Evangelisti","year":"2019","unstructured":"Evangelisti E, Yunusov T, Shenhav L, Schornack S (2019) N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans. BMC Microbiol 19:265","journal-title":"BMC Microbiol"},{"key":"3_CR32","doi-asserted-by":"publisher","first-page":"2402","DOI":"10.3389\/fmicb.2019.02402","volume":"10","author":"W Wang","year":"2019","unstructured":"Wang W, Xue Z, Miao J, Cai M, Zhang C, Li T, Zhang B, Tyler BM, Liu X (2019) PcMuORP1, an Oxathiapiprolin-resistance gene, functions as a novel selection marker for Phytophthora transformation and CRISPR\/Cas9 mediated genome editing. Front Microbiol 10:2402","journal-title":"Front Microbiol"}],"container-title":["Methods in Molecular Biology","Phytophthora"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4330-3_3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T15:02:38Z","timestamp":1735311758000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4330-3_3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,28]]},"ISBN":["9781071643297","9781071643303"],"references-count":32,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4330-3_3","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,28]]},"assertion":[{"value":"28 December 2024","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}