{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T18:43:07Z","timestamp":1793472187662,"version":"4.5.0"},"publisher-location":"New York, NY","reference-count":14,"publisher":"Springer US","isbn-type":[{"value":"9781071643853","type":"print"},{"value":"9781071643860","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4386-0_4","type":"book-chapter","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T03:04:49Z","timestamp":1741662289000},"page":"47-57","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolation of Oligodendrocyte Precursor Cells with Immunomagnetic Beads for Their Better Viability In Vitro and In Vivo"],"prefix":"10.1007","author":[{"given":"Paula","family":"G\u00f3mez-Mart\u00edn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Mac\u00edas-Castellano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Fern\u00e1ndez-G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estefan\u00eda","family":"S\u00e1nchez-Jim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Marchena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"de Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"4_CR1","first-page":"67","volume":"8","author":"P del R\u00edo-Hortega","year":"1919","unstructured":"del R\u00edo-Hortega P (1919) El \u201ctercer elemento\u201d de los centros nerviosos. I. La microgl\u00eda en estado normal. Bol Soc Esp Biol 8:67\u201382","journal-title":"Bol Soc Esp Biol"},{"key":"4_CR2","doi-asserted-by":"publisher","first-page":"4355","DOI":"10.1007\/s00018-013-1365-6","volume":"70","author":"F de Castro","year":"2013","unstructured":"de Castro F, Bribi\u00e1n A, Ortega MC (2013) Regulation of oligodendrocyte precursor migration during development, in adulthood and in pathology. Cell Mol Life Sci 70:4355\u20134368. https:\/\/doi.org\/10.1007\/s00018-013-1365-6","journal-title":"Cell Mol Life Sci"},{"key":"4_CR3","doi-asserted-by":"publisher","first-page":"1424","DOI":"10.3390\/cells8111424","volume":"8","author":"S Kuhn","year":"2019","unstructured":"Kuhn S, Gritti L, Crooks D et al (2019) Oligodendrocytes in development, myelin generation and beyond. Cells 8:1424. https:\/\/doi.org\/10.3390\/cells8111424","journal-title":"Cells"},{"key":"4_CR4","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S0166-2236(00)01691-X","volume":"24","author":"JM Levine","year":"2001","unstructured":"Levine JM, Reynolds R, Fawcett JW (2001) The oligodendrocyte precursor cell in health and disease. Trends Neurosci 24:39\u201347. https:\/\/doi.org\/10.1016\/S0166-2236(00)01691-X","journal-title":"Trends Neurosci"},{"key":"4_CR5","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1146\/annurev-neuro-061010-113629","volume":"34","author":"SPJ Fancy","year":"2011","unstructured":"Fancy SPJ, Chan JR, Baranzini SE et al (2011) Myelin regeneration: a recapitulation of development? Annu Rev Neurosci 34:21\u201343. https:\/\/doi.org\/10.1146\/annurev-neuro-061010-113629","journal-title":"Annu Rev Neurosci"},{"key":"4_CR6","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1038\/nrneurol.2012.200","volume":"8","author":"RJM Franklin","year":"2012","unstructured":"Franklin RJM, Ffrench-Constant C, Edgar JM et al (2012) Neuroprotection and repair in multiple sclerosis. Nat Rev Neurol 8:624\u2013634. https:\/\/doi.org\/10.1038\/nrneurol.2012.200","journal-title":"Nat Rev Neurol"},{"key":"4_CR7","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1146\/annurev.neuro.30.051606.094313","volume":"31","author":"BD Trapp","year":"2008","unstructured":"Trapp BD, Nave KA (2008) Multiple sclerosis: an immune or neurodegenerative disorder? Annu Rev Neurosci 31:247\u2013269. https:\/\/doi.org\/10.1146\/annurev.neuro.30.051606.094313","journal-title":"Annu Rev Neurosci"},{"key":"4_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081620","volume":"8","author":"EM Medina-Rodr\u00edguez","year":"2013","unstructured":"Medina-Rodr\u00edguez EM, Arenzana FJ, Bribi\u00e1n A et al (2013) Protocol to isolate a large amount of functional oligodendrocyte precursor cells from the cerebral cortex of adult mice and humans. PLoS One 8:e81620. https:\/\/doi.org\/10.1371\/journal.pone.0081620","journal-title":"PLoS One"},{"key":"4_CR9","doi-asserted-by":"publisher","first-page":"1681","DOI":"10.3390\/jcm9061681","volume":"9","author":"A Bribi\u00e1n","year":"2020","unstructured":"Bribi\u00e1n A, Medina-Rodr\u00edguez EM, Josa-Prado F et al (2020) Functional heterogeneity of mouse and human brain opcs: relevance for preclinical studies in multiple sclerosis. J Clin Med 9:1681. https:\/\/doi.org\/10.3390\/jcm9061681","journal-title":"J Clin Med"},{"key":"4_CR10","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1016\/j.bbamcr.2014.04.018","volume":"1843","author":"A Barateiro","year":"2014","unstructured":"Barateiro A, Fernandes A (2014) Temporal oligodendrocyte lineage progression: in vitro models of proliferation, differentiation and myelination. Biochim Biophys Acta 1843:1917\u20131929. https:\/\/doi.org\/10.1016\/j.bbamcr.2014.04.018","journal-title":"Biochim Biophys Acta"},{"key":"4_CR11","doi-asserted-by":"publisher","DOI":"10.1111\/cns.14552","volume":"30","author":"R Ben\u00edtez-Fern\u00e1ndez","year":"2023","unstructured":"Ben\u00edtez-Fern\u00e1ndez R, Josa-Prado F, S\u00e1nchez E et al (2023) Efficacy of a benzothiazole-based LRRK2 inhibitor in oligodendrocyte precursor cells and in a murine model of multiple sclerosis. CNS Neurosci Ther 30:e14552. https:\/\/doi.org\/10.1111\/cns.14552","journal-title":"CNS Neurosci Ther"},{"key":"4_CR12","doi-asserted-by":"publisher","unstructured":"Fern\u00e1ndez-G\u00f3mez B, Marchena MA, Pi\u00f1eiro D et al (2024) ApTOLL: a new therapeutic aptamer for cytoprotection and (re)myelination after multiple sclerosis. Br J Pharmacol\u00a0181:3263\u20133281. https:\/\/doi.org\/10.1111\/bph.16399","DOI":"10.1111\/bph.16399"},{"key":"4_CR13","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.jneumeth.2012.06.017","volume":"209","author":"TA Dincman","year":"2012","unstructured":"Dincman TA, Beare JE, Ohri SS et al (2012) Isolation of cortical mouse oligodendrocyte precursor cells. J Neurosci Methods 209:219\u2013226. https:\/\/doi.org\/10.1016\/j.jneumeth.2012.06.017","journal-title":"J Neurosci Methods"},{"key":"4_CR14","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.1002\/glia.24540","volume":"72","author":"S Nocera","year":"2024","unstructured":"Nocera S, Marchena M, Fern\u00e1ndez-G\u00f3mez B et al (2024) Activation of Shh\/Smo is sufficient to maintain oligodendrocyte precursor cells in an undifferentiated state and is not necessary for myelin formation and (re)myelination. Glia 72:1469. https:\/\/doi.org\/10.1002\/glia.24540","journal-title":"Glia"}],"container-title":["Methods in Molecular Biology","Neural Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4386-0_4","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T03:04:51Z","timestamp":1741662291000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4386-0_4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9781071643853","9781071643860"],"references-count":14,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4386-0_4","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"12 March 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}