{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T04:10:31Z","timestamp":1747973431098,"version":"3.41.0"},"publisher-location":"New York, NY","reference-count":8,"publisher":"Springer US","isbn-type":[{"value":"9781071645574","type":"print"},{"value":"9781071645581","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4558-1_12","type":"book-chapter","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T11:13:35Z","timestamp":1747912415000},"page":"157-186","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Robust Quantitative Multiplex PCR for T-cell Receptor Repertoire Sequencing from Formalin-Fixed Paraffin-Embedded Samples"],"prefix":"10.1007","author":[{"given":"Gayathri","family":"Nageswaran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Byrne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Nenclares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Cowley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Poignant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor","family":"Graham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Marie","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benny","family":"Chain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,23]]},"reference":[{"key":"12_CR1","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1038\/s41587-020-0656-3","volume":"39","author":"P Barennes","year":"2021","unstructured":"Barennes P, Quiniou V, Shugay M et al (2021) Benchmarking of T cell receptor repertoire profiling methods reveals large systematic biases. Nat Biotechnol 39:236\u2013245","journal-title":"Nat Biotechnol"},{"key":"12_CR2","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1093\/bioinformatics\/btaa758","volume":"37","author":"T Peacock","year":"2021","unstructured":"Peacock T, Heather JM, Ronel T et al (2021) Decombinator V4: an improved AIRR compliant-software package for T-cell receptor sequence annotation? Bioinformatics 37:876\u2013878","journal-title":"Bioinformatics"},{"key":"12_CR3","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/S0022-1759(02)00336-8","volume":"270","author":"A Gallard","year":"2002","unstructured":"Gallard A, Foucras G, Coureau C et al (2002) Tracking T cell clonotypes in complex T lymphocyte populations by real-time quantitative PCR using fluorogenic complementarity-determining region-3-specific probes. J Immunol Methods 270:269\u2013280","journal-title":"J Immunol Methods"},{"key":"12_CR4","doi-asserted-by":"publisher","first-page":"1769","DOI":"10.4049\/jimmunol.141.5.1769","volume":"141","author":"T Lindsten","year":"1988","unstructured":"Lindsten T, June CH, Thompson CB (1988) Transcription of T cell antigen receptor genes is induced by protein kinase C activation. J Immunol 141:1769\u20131774","journal-title":"J Immunol"},{"key":"12_CR5","doi-asserted-by":"publisher","first-page":"1867","DOI":"10.1002\/j.1460-2075.1990.tb08312.x","volume":"9","author":"F Paillard","year":"1990","unstructured":"Paillard F, Sterkers G, Vaquero C (1990) Transcriptional and post-transcriptional regulation of TcR, CD4 and CD8 gene expression during activation of normal human T lymphocytes. EMBO J 9:1867\u20131872","journal-title":"EMBO J"},{"key":"12_CR6","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/0006-291X(92)90789-N","volume":"186","author":"F Paillard","year":"1992","unstructured":"Paillard F, Sterkers G, Vaquero C (1992) Correlation between up-regulation of lymphokine mRNA and down-regulation of TcR, CD4, CD8 and lck mRNA as shown by the effect of CsA on activated T lymphocytes. Biochem Biophys Res Commun 186:603\u2013611","journal-title":"Biochem Biophys Res Commun"},{"key":"12_CR7","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.3389\/fimmu.2017.01267","volume":"8","author":"T Oakes","year":"2017","unstructured":"Oakes T, Heather JM, Best K et al (2017) Quantitative characterization of the T cell receptor repertoire of Na\u00efve and memory subsets using an integrated experimental and computational pipeline which is robust, economical, and versatile. Front Immunol 8:1267","journal-title":"Front Immunol"},{"key":"12_CR8","doi-asserted-by":"publisher","first-page":"2241","DOI":"10.1038\/s41375-019-0496-7","volume":"33","author":"M Br\u00fcggemann","year":"2019","unstructured":"Br\u00fcggemann M, Kotrov\u00e1 M, Knecht H et al (2019) Standardized next-generation sequencing of immunoglobulin and T-cell receptor gene recombinations for MRD marker identification in acute lymphoblastic leukaemia; a EuroClonality-NGS validation study. Leukemia 33:2241\u20132253","journal-title":"Leukemia"}],"container-title":["Methods in Molecular Biology","Cancer Immunosurveillance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4558-1_12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T11:13:35Z","timestamp":1747912415000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4558-1_12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9781071645574","9781071645581"],"references-count":8,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4558-1_12","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"23 May 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}