{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T04:09:02Z","timestamp":1749701342700,"version":"3.41.0"},"publisher-location":"New York, NY","reference-count":38,"publisher":"Springer US","isbn-type":[{"value":"9781071646267","type":"print"},{"value":"9781071646274","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4627-4_17","type":"book-chapter","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T09:27:46Z","timestamp":1749634066000},"page":"199-215","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adhesion of Pseudomonas aeruginosa to Human Outer Layer Airway Mucins"],"prefix":"10.1007","author":[{"given":"Burkhard","family":"T\u00fcmmler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"17_CR1","first-page":"429","volume":"125","author":"NA McNamara","year":"2000","unstructured":"McNamara NA, Sack RA, Fleiszig MJ (2000) Mucin\u2014bacterial binding assays. Meth Mol Biol 125:429\u2013437","journal-title":"Meth Mol Biol"},{"key":"17_CR2","doi-asserted-by":"publisher","first-page":"2233","DOI":"10.1056\/NEJMra0910061","volume":"363","author":"JV Fahy","year":"2020","unstructured":"Fahy JV, Dickey BF (2020) Airway mucus function and dysfunction. N Engl J Med 363:2233\u20132247. https:\/\/doi.org\/10.1056\/NEJMra0910061","journal-title":"N Engl J Med"},{"key":"17_CR3","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.addr.2008.09.012","volume":"61","author":"SK Lai","year":"2009","unstructured":"Lai SK, Wang YY, Wirtz D et al (2009) Micro- and macrorheology of mucus. Adv Drug Deliv Rev 61:86\u2013100. https:\/\/doi.org\/10.1016\/j.addr.2008.09.012","journal-title":"Adv Drug Deliv Rev"},{"key":"17_CR4","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.3390\/ijms25031933","volume":"25","author":"M Abrami","year":"2024","unstructured":"Abrami M, Biasin A, Tescione F et al (2024) Mucus structure, viscoelastic properties, and composition in chronic respiratory diseases. Int J Mol Sci 25:1933. https:\/\/doi.org\/10.3390\/ijms25031933","journal-title":"Int J Mol Sci"},{"key":"17_CR5","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1146\/annurev-biochem-011520-105053","volume":"89","author":"GC Hansson","year":"2020","unstructured":"Hansson GC (2020) Mucins and the microbiome. Annu Rev Biochem 89:769\u2013793. https:\/\/doi.org\/10.1146\/annurev-biochem-011520-105053","journal-title":"Annu Rev Biochem"},{"issue":"Suppl 3","key":"17_CR6","doi-asserted-by":"publisher","first-page":"S159","DOI":"10.1513\/AnnalsATS.201804-238AW","volume":"15","author":"A Ermund","year":"2018","unstructured":"Ermund A, Trillo-Muyo S, Hansson GC (2018) Assembly, release, and transport of airway mucins in pigs and humans. Ann Am Thorac Soc 15(Suppl 3):S159\u2013S163. https:\/\/doi.org\/10.1513\/AnnalsATS.201804-238AW","journal-title":"Ann Am Thorac Soc"},{"key":"17_CR7","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1038\/s41522-020-00171-7","volume":"6","author":"MM Pust","year":"2020","unstructured":"Pust MM, Wiehlmann L, Davenport C et al (2020) The human respiratory tract microbial community structures in healthy and cystic fibrosis infants. NPJ Biofilms Microbiomes 6:61. https:\/\/doi.org\/10.1038\/s41522-020-00171-7","journal-title":"NPJ Biofilms Microbiomes"},{"key":"17_CR8","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03633-22","volume":"11","author":"K Pienkowska","year":"2023","unstructured":"Pienkowska K, Pust MM, Gessner M et al (2023) The cystic fibrosis upper and lower airway metagenome. Microbiol Spectr 11:e0363322. https:\/\/doi.org\/10.1128\/spectrum.03633-22","journal-title":"Microbiol Spectr"},{"key":"17_CR9","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1038\/nature12807","volume":"505","author":"MG Roy","year":"2014","unstructured":"Roy MG, Livraghi-Butrico A, Fletcher AA et al (2014) Muc5b is required for airway defence. Nature 505:412\u2013416. https:\/\/doi.org\/10.1038\/nature12807","journal-title":"Nature"},{"key":"17_CR10","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/j.bbrc.2017.08.113","volume":"492","author":"A Ermund","year":"2017","unstructured":"Ermund A, Meiss LN, Rodriguez-Pineiro AM et al (2017) The normal trachea is cleaned by MUC5B mucin bundles from the submucosal glands coated with the MUC5AC mucin. Biochem Biophys Res Commun 492:331\u2013337. https:\/\/doi.org\/10.1016\/j.bbrc.2017.08.113","journal-title":"Biochem Biophys Res Commun"},{"key":"17_CR11","doi-asserted-by":"publisher","first-page":"1800457","DOI":"10.1183\/13993003.00457-2018","volume":"52","author":"A Ermund","year":"2018","unstructured":"Ermund A, Meiss LN, Dolan B et al (2018) The mucus bundles responsible for airway cleaning are retained in cystic fibrosis and by cholinergic stimulation. Eur Respir J 52:1800457. https:\/\/doi.org\/10.1183\/13993003.00457-2018","journal-title":"Eur Respir J"},{"key":"17_CR12","doi-asserted-by":"publisher","first-page":"pgad388","DOI":"10.1093\/pnasnexus\/pgad388","volume":"2","author":"MF Bos","year":"2023","unstructured":"Bos MF, Ermund A, Hansson GC et al (2023) Goblet cell interactions reorient bundled mucus strands for efficient airway clearance. PNAS Nexus 2:pgad388. https:\/\/doi.org\/10.1093\/pnasnexus\/pgad388","journal-title":"PNAS Nexus"},{"key":"17_CR13","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.120994","volume":"3","author":"JA Fern\u00e1ndez-Blanco","year":"2018","unstructured":"Fern\u00e1ndez-Blanco JA, Fakih D, Arike L et al (2018) Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs. JCI Insight 3:e120994. https:\/\/doi.org\/10.1172\/jci.insight.120994","journal-title":"JCI Insight"},{"key":"17_CR14","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.chest.2017.11.008","volume":"154","author":"J Ma","year":"2018","unstructured":"Ma J, Rubin BK, Voynow JA (2018) Mucins, mucus, and goblet cells. Chest 154:169\u2013176. https:\/\/doi.org\/10.1016\/j.chest.2017.11.008","journal-title":"Chest"},{"key":"17_CR15","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1165\/rcmb.2021-0064OC","volume":"65","author":"M Padra","year":"2021","unstructured":"Padra M, Benktander J, Padra JT et al (2021) Mucin binding to Moraxella catarrhalis during airway inflammation is dependent on sialic acid. Am J Respir Cell Mol Biol 65:593\u2013602. https:\/\/doi.org\/10.1165\/rcmb.2021-0064OC","journal-title":"Am J Respir Cell Mol Biol"},{"key":"17_CR16","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00039-22","volume":"13","author":"LR Burcham","year":"2022","unstructured":"Burcham LR, Bath JR, Werlang CA et al (2022) Role of MUC5B during group B streptococcal vaginal colonization. MBio 13:e0003922. https:\/\/doi.org\/10.1128\/mbio.00039-22","journal-title":"MBio"},{"key":"17_CR17","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/978-3-031-08491-1_10","volume":"1386","author":"B T\u00fcmmler","year":"2022","unstructured":"T\u00fcmmler B (2022) What makes Pseudomonas aeruginosa a pathogen? Adv Exp Med Biol 1386:283\u2013301. https:\/\/doi.org\/10.1007\/978-3-031-08491-1_10","journal-title":"Adv Exp Med Biol"},{"key":"17_CR18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2023.104532","volume":"90","author":"J Botelho","year":"2023","unstructured":"Botelho J, T\u00fcffers L, Fuss J et al (2023) Phylogroup-specific variation shapes the clustering of antimicrobial resistance genes and defence systems across regions of genome plasticity in Pseudomonas aeruginosa. EBioMedicine 90:104532. https:\/\/doi.org\/10.1016\/j.ebiom.2023.104532","journal-title":"EBioMedicine"},{"key":"17_CR19","doi-asserted-by":"publisher","first-page":"124","DOI":"10.12688\/wellcomeopenres.14826.1","volume":"3","author":"KA Jolley","year":"2018","unstructured":"Jolley KA, Bray JE, Maiden MCJ (2018) Open-access bacterial population genomics: BIGSdb software, the PubMLST.Org website and their applications. Wellcome Open Res 3:124. https:\/\/doi.org\/10.12688\/wellcomeopenres.14826.1","journal-title":"Wellcome Open Res"},{"key":"17_CR20","doi-asserted-by":"publisher","first-page":"1724","DOI":"10.1021\/bm3001292","volume":"13","author":"O Lieleg","year":"2012","unstructured":"Lieleg O, Lieleg C, Bloom J et al (2012) Mucin biopolymers as broad-spectrum antiviral agents. Biomacromolecules 13:1724\u20131732. https:\/\/doi.org\/10.1021\/bm3001292","journal-title":"Biomacromolecules"},{"key":"17_CR21","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1016\/s0142-9612(96)00180-9","volume":"18","author":"J Kocevar-Nared","year":"1997","unstructured":"Kocevar-Nared J, Kristl J, Smid-Korbar J (1997) Comparative rheological investigation of crude gastric mucin and natural gastric mucus. Biomaterials 18:677\u2013681. https:\/\/doi.org\/10.1016\/s0142-9612(96)00180-9","journal-title":"Biomaterials"},{"key":"17_CR22","doi-asserted-by":"publisher","first-page":"44932","DOI":"10.1039\/c6ra07424c","volume":"6","author":"VJ Sch\u00f6mig","year":"2016","unstructured":"Sch\u00f6mig VJ, K\u00e4sdorf BT, Scholz C et al (2016) An optimized purification process for porcine gastric mucin with preservation of its native functional properties. RSC Adv 6:44932. https:\/\/doi.org\/10.1039\/c6ra07424c","journal-title":"RSC Adv"},{"key":"17_CR23","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/s0008-6215(00)81856-x","volume":"105","author":"CE Snyder","year":"1982","unstructured":"Snyder CE, Nadziejko CE, Herp A (1982) Isolation of bronchial mucins from cystic fibrosis sputum by use of citraconic anhydride. Carbohydr Res 105:87\u201393. https:\/\/doi.org\/10.1016\/s0008-6215(00)81856-x","journal-title":"Carbohydr Res"},{"key":"17_CR24","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1007\/978-1-0716-3670-1_11","volume":"2763","author":"G Radicioni","year":"2024","unstructured":"Radicioni G, Kesimer M (2024) Quantitation of MUC5AC and MUC5B by stable isotope labeling mass spectrometry. Methods Mol Biol 2763:125\u2013136. https:\/\/doi.org\/10.1007\/978-1-0716-3670-1_11","journal-title":"Methods Mol Biol"},{"key":"17_CR25","unstructured":"Global Initiative for Chronic Obstructive Lung Disease (GOLD) (2023) Global strategy for prevention, diagnosis and management of COPD: 2023 Report. https:\/\/goldcopd.org\/2023-gold-report-2\/"},{"key":"17_CR26","doi-asserted-by":"publisher","first-page":"2300239","DOI":"10.1183\/13993003.00239-2023","volume":"61","author":"A Agust\u00ed","year":"2023","unstructured":"Agust\u00ed A, Celli BR, Criner GJ et al (2023) Global initiative for chronic obstructive lung disease 2023 report: GOLD executive summary. Eur Respir J 61:2300239. https:\/\/doi.org\/10.1183\/13993003.00239-2023","journal-title":"Eur Respir J"},{"key":"17_CR27","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/S2213-2600(23)00093-0","volume":"11","author":"JD Chalmers","year":"2023","unstructured":"Chalmers JD, Polverino E, Crichton ML et al (2023) Bronchiectasis in Europe: data on disease characteristics from the European bronchiectasis registry (EMBARC). Lancet Respir Med 11:637\u2013649. https:\/\/doi.org\/10.1016\/S2213-2600(23)00093-0","journal-title":"Lancet Respir Med"},{"key":"17_CR28","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1164\/rccm.201309-1575OC","volume":"189","author":"JD Chalmers","year":"2014","unstructured":"Chalmers JD, Goeminne P, Aliberti S et al (2014) The bronchiectasis severity index. An international derivation and validation study. Am J Respir Crit Care Med 189:576\u2013585. https:\/\/doi.org\/10.1164\/rccm.201309-1575OC","journal-title":"Am J Respir Crit Care Med"},{"key":"17_CR29","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1056\/NEJMra2216474","volume":"389","author":"H Grasemann","year":"2023","unstructured":"Grasemann H, Ratjen F (2023) Cystic fibrosis. N Engl J Med 389:1693\u20131707. https:\/\/doi.org\/10.1056\/NEJMra2216474","journal-title":"N Engl J Med"},{"key":"17_CR30","doi-asserted-by":"publisher","first-page":"64","DOI":"10.1186\/1750-1172-7-64","volume":"7","author":"PY Bo\u00eblle","year":"2012","unstructured":"Bo\u00eblle PY, Viviani L, Busson PF et al (2012) Reference percentiles for FEV(1) and BMI in European children and adults with cystic fibrosis. Orphanet J Rare Dis 7:64. https:\/\/doi.org\/10.1186\/1750-1172-7-64","journal-title":"Orphanet J Rare Dis"},{"key":"17_CR31","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.101784","volume":"298","author":"A Konstantinidi","year":"2022","unstructured":"Konstantinidi A, Nason R, \u010caval T et al (2022) Exploring the glycosylation of mucins by use of O-glycodomain reporters recombinantly expressed in glycoengineered HEK293 cells. J Biol Chem 298:101784. https:\/\/doi.org\/10.1016\/j.jbc.2022.101784","journal-title":"J Biol Chem"},{"key":"17_CR32","doi-asserted-by":"publisher","first-page":"7736","DOI":"10.1074\/jbc.M111690200","volume":"277","author":"TA Gerken","year":"2002","unstructured":"Gerken TA, Gilmore M, Zhang J (2002) Determination of the site-specific oligosaccharide distribution of the O-glycans attached to the porcine submaxillary mucin tandem repeat. Further evidence for the modulation of O-glycans side chain structures by peptide sequence. J Biol Chem 277:7736\u20137751. https:\/\/doi.org\/10.1074\/jbc.M111690200","journal-title":"J Biol Chem"},{"key":"17_CR33","doi-asserted-by":"publisher","first-page":"8825","DOI":"10.1021\/acs.analchem.3c00127","volume":"95","author":"GM Vos","year":"2023","unstructured":"Vos GM, Weber J, Sweet IR et al (2023) Oxidative release of O-Glycans under neutral conditions for analysis of Glycoconjugates having base-sensitive substituents. Anal Chem 95:8825\u20138833. https:\/\/doi.org\/10.1021\/acs.analchem.3c00127","journal-title":"Anal Chem"},{"key":"17_CR34","volume-title":"MIQ 24: Atemwegsinfektionen bei Mukoviszidose","author":"M Hogardt","year":"2019","unstructured":"Hogardt M, Kahl BC, Besier S et al (2019) MIQ 24: Atemwegsinfektionen bei Mukoviszidose, 2nd edn. Elsevier","edition":"2"},{"key":"17_CR35","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00143-24","volume":"12","author":"L Tueffers","year":"2024","unstructured":"Tueffers L, Batra A, Zimmermann J et al (2024) Variation in the response to antibiotics and life-history across the major Pseudomonas aeruginosa clone type (mPact) panel. Microbiol Spectr 12:e0014324. https:\/\/doi.org\/10.1128\/spectrum.00143-24","journal-title":"Microbiol Spectr"},{"key":"17_CR36","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1002\/mbo3.141","volume":"2","author":"A De Soyza","year":"2013","unstructured":"De Soyza A, Hall AJ, Mahenthiralingam E et al (2013) Developing an international Pseudomonas aeruginosa reference panel. Microbiology 2:1010\u20131023. https:\/\/doi.org\/10.1002\/mbo3.141","journal-title":"Microbiology"},{"key":"17_CR37","doi-asserted-by":"publisher","unstructured":"Freschi L, Bertelli C, Jeukens J et al (2018) Genomic characterisation of an international Pseudomonas aeruginosa reference panel indicates that the two major groups draw upon distinct mobile gene pools. FEMS Microbiol Lett 365(14). https:\/\/doi.org\/10.1093\/femsle\/fny120","DOI":"10.1093\/femsle\/fny120"},{"issue":"D1","key":"17_CR38","doi-asserted-by":"publisher","first-page":"D646","DOI":"10.1093\/nar\/gkv1227","volume":"44","author":"GL Winsor","year":"2016","unstructured":"Winsor GL, Griffiths EJ, Lo R et al (2016) Enhanced annotations and features for comparing thousands of pseudomonas genomes in the pseudomonas genome database. Nucleic Acids Res 44(D1):D646\u2013D653. https:\/\/doi.org\/10.1093\/nar\/gkv1227","journal-title":"Nucleic Acids Res"}],"container-title":["Methods in Molecular Biology","Dynamics of Bacteria-Mucus Interactions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4627-4_17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T09:27:50Z","timestamp":1749634070000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4627-4_17"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9781071646267","9781071646274"],"references-count":38,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4627-4_17","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"12 June 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}