{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T10:00:28Z","timestamp":1784973628435,"version":"3.55.0"},"publisher-location":"New York, NY","reference-count":30,"publisher":"Springer US","isbn-type":[{"value":"9781071646977","type":"print"},{"value":"9781071646984","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4698-4_9","type":"book-chapter","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:29:39Z","timestamp":1751416179000},"page":"183-195","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Density Gradient-Based Separation of Testicular Cell Types Using a STA-PUT Apparatus"],"prefix":"10.1007","author":[{"given":"Mirella L.","family":"Meyer-Ficca","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph G.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"9_CR1","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1002\/jemt.20783","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo L, Pelletier R-M, Cyr DG et al (2010) Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 1: background to spermatogenesis, spermatogonia, and spermatocytes. Microsc Res Tech 73:241\u2013278","journal-title":"Microsc Res Tech"},{"key":"9_CR2","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1002\/jemt.20787","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo L, Pelletier R-M, Cyr DG et al (2010) Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 2: changes in spermatid organelles associated with development of spermatozoa. Microsc Res Tech 73:279\u2013319","journal-title":"Microsc Res Tech"},{"key":"9_CR3","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1002\/jemt.20786","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo L, Pelletier R-M, Cyr DG et al (2010) Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 5: intercellular junctions and contacts between germs cells and Sertoli cells and their regulatory interactions, testicular cholesterol, and genes\/proteins associated with more than one germ cell generation. Microsc Res Tech 73:409\u2013494","journal-title":"Microsc Res Tech"},{"key":"9_CR4","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1002\/jemt.20784","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo L, Pelletier R-M, Cyr DG et al (2010) Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 3: developmental changes in spermatid flagellum and cytoplasmic droplet and interaction of sperm with the zona pellucida and egg plasma membrane. Microsc Res Tech 73:320\u2013363","journal-title":"Microsc Res Tech"},{"key":"9_CR5","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1002\/jemt.20785","volume":"73","author":"L Hermo","year":"2010","unstructured":"Hermo L, Pelletier R-M, Cyr DG et al (2010) Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. Part 4: intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane\/voltage-gated channels, methylation\/acetylation, and transcription factors. Microsc Res Tech 73:364\u2013408","journal-title":"Microsc Res Tech"},{"key":"9_CR6","doi-asserted-by":"publisher","first-page":"1106111","DOI":"10.3389\/fcell.2023.1106111","volume":"11","author":"AY Kulibin","year":"2023","unstructured":"Kulibin AY, Malolina EA (2023) In vitro spermatogenesis: in search of fully defined conditions. Front Cell Dev Biol 11:1106111","journal-title":"Front Cell Dev Biol"},{"key":"9_CR7","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/s41585-023-00723-4","volume":"20","author":"M Robinson","year":"2023","unstructured":"Robinson M, Sparanese S, Witherspoon L et al (2023) Human in vitro spermatogenesis as a regenerative therapy\u2014where do we stand? Nat Rev Urol 20:461\u2013479","journal-title":"Nat Rev Urol"},{"key":"9_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1262\/jrd.2023-093","volume":"70","author":"T Ogawa","year":"2024","unstructured":"Ogawa T, Matsumura T, Yao T et al (2024) Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control. J Reprod Dev 70:1\u20139","journal-title":"J Reprod Dev"},{"key":"9_CR9","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/0014-4827(73)90507-7","volume":"79","author":"ML Meistrich","year":"1973","unstructured":"Meistrich ML, Bruce WR, Clermont Y (1973) Cellular composition of fractions of mouse testis cells following velocity sedimentation separation. Exp Cell Res 79:213\u2013227","journal-title":"Exp Cell Res"},{"key":"9_CR10","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/0012-1606(76)90262-1","volume":"49","author":"LJ Romrell","year":"1976","unstructured":"Romrell LJ, Bellv\u00e9 AR, Fawcett DW (1976) Separation of mouse spermatogenic cells by sedimentation velocity. Dev Biol 49:119\u2013131","journal-title":"Dev Biol"},{"key":"9_CR11","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1083\/jcb.74.1.68","volume":"74","author":"A Bellve","year":"1977","unstructured":"Bellve A, Cavicchia J, Millette C et al (1977) Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization. J Cell Biol 74:68\u201385","journal-title":"J Cell Biol"},{"key":"9_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101540","volume":"3","author":"Y Cao","year":"2022","unstructured":"Cao Y, Meng L, Cheng Y et al (2022) Optimized protocol for isolation of germ cells from mouse testis by centrifugal elutriation. STAR Protoc 3:101540","journal-title":"STAR Protoc"},{"key":"9_CR13","doi-asserted-by":"publisher","first-page":"341","DOI":"10.2144\/000113764","volume":"51","author":"Y-F Chang","year":"2011","unstructured":"Chang Y-F, Lee-Chang JS, Panneerdoss S et al (2011) Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis. BioTechniques 51:341\u2013342, 344","journal-title":"BioTechniques"},{"key":"9_CR14","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.theriogenology.2023.09.023","volume":"213","author":"B Li","year":"2024","unstructured":"Li B, Zhao X, Jin T et al (2024) Efficient isolation and purification of spermatogonia, spermatocytes, and spermatids from mice, piglets, and adult boars using an optimized STA-PUT method. Theriogenology 213:97\u2013108","journal-title":"Theriogenology"},{"key":"9_CR15","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.3390\/ijms22031151","volume":"22","author":"R Rodr\u00edguez-Casuriaga","year":"2021","unstructured":"Rodr\u00edguez-Casuriaga R, Geisinger A (2021) Contributions of flow cytometry to the molecular study of spermatogenesis in mammals. Int J Mol Sci 22:1151","journal-title":"Int J Mol Sci"},{"key":"9_CR16","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1111\/andr.13211","volume":"10","author":"M Ji","year":"2022","unstructured":"Ji M, Chen D, Zhao X et al (2022) Isolation of Leydig cells from adult rat testes by magnetic-activated cell sorting protocol based on prolactin receptor expression. Andrology 10:1197\u20131207","journal-title":"Andrology"},{"key":"9_CR17","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1095\/biolreprod.109.078550","volume":"82","author":"Z He","year":"2010","unstructured":"He Z, Kokkinaki M, Jiang J et al (2010) Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363\u2013372","journal-title":"Biol Reprod"},{"key":"9_CR18","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1016\/j.fertnstert.2014.04.036","volume":"102","author":"H Valli","year":"2014","unstructured":"Valli H, Sukhwani M, Dovey SL et al (2014) Fluorescence- and magnetic-activated cell sorting strategies to isolate and enrich human spermatogonial stem cells. Fertil Steril 102:566\u2013580.e7","journal-title":"Fertil Steril"},{"key":"9_CR19","first-page":"721","volume":"55","author":"TD Lee","year":"1985","unstructured":"Lee TD, Shanahan F, Miller HR et al (1985) Intestinal mucosal mast cells: isolation from rat lamina propria and purification using unit gravity velocity sedimentation. Immunology 55:721\u2013728","journal-title":"Immunology"},{"key":"9_CR20","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1111\/j.1768-322X.1984.tb00231.x","volume":"49","author":"H Dupont","year":"1983","unstructured":"Dupont H, Dupont MA, Bricaud H et al (1983) Megakaryocytes separation in homogeneous classes by unit gravity sedimentation: physico-chemical, ultrastructural and cytophotometric characterizations. Biol Cell 49:137\u2013144","journal-title":"Biol Cell"},{"key":"9_CR21","doi-asserted-by":"publisher","first-page":"1567","DOI":"10.1111\/jcmm.17197","volume":"26","author":"H Yuan","year":"2022","unstructured":"Yuan H, Sun J, Wang S et al (2022) Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey. J Cell Mol Med 26:1567\u20131578","journal-title":"J Cell Mol Med"},{"key":"9_CR22","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.reprotox.2010.11.010","volume":"31","author":"G Collodel","year":"2011","unstructured":"Collodel G, Federico MG, Geminiani M et al (2011) Effect of trans-resveratrol on induced oxidative stress in human sperm and in rat germinal cells. Reprod Toxicol 31:239\u2013246","journal-title":"Reprod Toxicol"},{"key":"9_CR23","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.anireprosci.2018.04.067","volume":"193","author":"MA Shah","year":"2018","unstructured":"Shah MA, Xu C, Wu S et al (2018) Isolation and characterization of spermatogenic cells from cattle, yak and cattleyak. Anim Reprod Sci 193:182\u2013190","journal-title":"Anim Reprod Sci"},{"key":"9_CR24","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1530\/REP-17-0441","volume":"154","author":"X Chen","year":"2017","unstructured":"Chen X, Che D, Zhang P et al (2017) Profiling of miRNAs in porcine germ cells during spermatogenesis. Reproduction 154:789\u2013798","journal-title":"Reproduction"},{"key":"9_CR25","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1095\/biolreprod.114.125757","volume":"92","author":"BP Hermann","year":"2015","unstructured":"Hermann BP, Mutoji KN, Velte EK et al (2015) Transcriptional and translational heterogeneity among neonatal mouse spermatogonia. Biol Reprod 92:54","journal-title":"Biol Reprod"},{"key":"9_CR26","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/978-1-61779-436-0_2","volume":"825","author":"G Manku","year":"2012","unstructured":"Manku G, Mazer M, Culty M (2012) Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis. Methods Mol Biol 825:17\u201329","journal-title":"Methods Mol Biol"},{"key":"9_CR27","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1007\/978-1-61779-436-0_6","volume":"825","author":"Y Wang","year":"2012","unstructured":"Wang Y, Culty M (2012) Preparation of enriched mouse syncitia-free pachytene spermatocyte cell suspensions. Methods Mol Biol 825:67\u201374","journal-title":"Methods Mol Biol"},{"key":"9_CR28","doi-asserted-by":"crossref","unstructured":"Da Ros M, Lehtiniemi T, Olotu O et al (2019) Enrichment of pachytene spermatocytes and spermatids from mouse testes using standard laboratory equipment. J Vis Exp (151):60271","DOI":"10.3791\/60271-v"},{"key":"9_CR29","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/0076-6879(93)25009-Q","volume":"225","author":"AR Bellve","year":"1993","unstructured":"Bellve AR (1993) Purification, culture, and fractionation of spermatogenic cells. Methods Enzymol 225:84\u2013113","journal-title":"Methods Enzymol"},{"key":"9_CR30","doi-asserted-by":"crossref","unstructured":"Bryant JM, Meyer-Ficca ML, Dang VM et al (2013) Separation of spermatogenic cell types using STA-PUT velocity sedimentation. J Vis Exp (80):50648","DOI":"10.3791\/50648"}],"container-title":["Methods in Molecular Biology","Spermatogenesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4698-4_9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T09:04:53Z","timestamp":1784970293000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4698-4_9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9781071646977","9781071646984"],"references-count":30,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4698-4_9","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"3 July 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}