{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T15:24:34Z","timestamp":1789140274375,"version":"build-2803163510"},"publisher-location":"New York, NY","reference-count":12,"publisher":"Springer US","isbn-type":[{"value":"9781071647257","type":"print"},{"value":"9781071647264","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025]]},"DOI":"10.1007\/978-1-0716-4726-4_8","type":"book-chapter","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T14:58:40Z","timestamp":1753282720000},"page":"103-119","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["5\u2032RACE Method for RNA-Based Sequencing of the Mouse Immunoglobulin Repertoire"],"prefix":"10.1007","author":[{"given":"Sandy","family":"Al Hayek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Prait\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4812-6596","authenticated-orcid":false,"given":"Jean-Marie","family":"Lambert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Bender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Delpy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"8_CR1","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1038\/381751a0","volume":"381","author":"K Rajewsky","year":"1996","unstructured":"Rajewsky K (1996) Clonal selection and learning in the antibody system. Nature 381:751\u2013758. https:\/\/doi.org\/10.1038\/381751a0","journal-title":"Nature"},{"key":"8_CR2","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1146\/annurev.immunol.23.021704.115830","volume":"24","author":"D Jung","year":"2006","unstructured":"Jung D, Giallourakis C, Mostoslavsky R, Alt FW (2006) Mechanism and control of V(D)J recombination at the immunoglobulin heavy chain locus. Annu Rev Immunol 24:541\u2013570. https:\/\/doi.org\/10.1146\/annurev.immunol.23.021704.115830","journal-title":"Annu Rev Immunol"},{"key":"8_CR3","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1084\/jem.189.10.1611","volume":"189","author":"AV Popov","year":"1999","unstructured":"Popov AV, Zou X, Xian J et al (1999) A human immunoglobulin \u03bb locus is similarly well expressed in mice and humans. J Exp Med 189:1611\u20131620","journal-title":"J Exp Med"},{"key":"8_CR4","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1146\/annurev.immunol.20.090501.112049","volume":"20","author":"T Honjo","year":"2002","unstructured":"Honjo T, Kinoshita K, Muramatsu M (2002) Molecular mechanism of class switch recombination: linkage with somatic hypermutation. Annu Rev Immunol 20:165\u2013196. https:\/\/doi.org\/10.1146\/annurev.immunol.20.090501.112049","journal-title":"Annu Rev Immunol"},{"key":"8_CR5","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/S0065-2776(04)86002-4","volume":"86","author":"DD Dudley","year":"2005","unstructured":"Dudley DD, Chaudhuri J, Bassing CH, Alt FW (2005) Mechanism and control of V(D)J recombination versus class switch recombination: similarities and differences. Adv Immunol 86:43\u2013112. https:\/\/doi.org\/10.1016\/S0065-2776(04)86002-4","journal-title":"Adv Immunol"},{"key":"8_CR6","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1038\/nbt.2782","volume":"32","author":"G Georgiou","year":"2014","unstructured":"Georgiou G, Ippolito GC, Beausang J et al (2014) The promise and challenge of high-throughput sequencing of the antibody repertoire. Nat Biotechnol 32:158\u2013168. https:\/\/doi.org\/10.1038\/nbt.2782","journal-title":"Nat Biotechnol"},{"key":"8_CR7","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.641692","volume":"12","author":"C Ouk","year":"2021","unstructured":"Ouk C, Roland L, Gachard N et al (2021) Continuous MYD88 activation is associated with expansion and then transformation of IgM differentiating plasma cells. Front Immunol 12:641692. https:\/\/doi.org\/10.3389\/fimmu.2021.641692","journal-title":"Front Immunol"},{"key":"8_CR8","doi-asserted-by":"publisher","first-page":"1114","DOI":"10.1038\/s41423-023-01069-y","volume":"20","author":"M Thomas","year":"2023","unstructured":"Thomas M, Bruzeau C, Martin OA et al (2023) A dual function for the chromatin organizer special A-T rich binding protein 1 in B-lineage cells. Cell Mol Immunol 20:1114\u20131126. https:\/\/doi.org\/10.1038\/s41423-023-01069-y","journal-title":"Cell Mol Immunol"},{"key":"8_CR9","doi-asserted-by":"publisher","first-page":"1750","DOI":"10.1182\/blood.2019004197","volume":"135","author":"S Bender","year":"2020","unstructured":"Bender S, Javaugue V, Saintamand A et al (2020) Immunoglobulin variable domain high-throughput sequencing reveals specific novel mutational patterns in POEMS syndrome. Blood 135:1750\u20131758. https:\/\/doi.org\/10.1182\/blood.2019004197","journal-title":"Blood"},{"key":"8_CR10","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1038\/nprot.2016.093","volume":"11","author":"MA Turchaninova","year":"2016","unstructured":"Turchaninova MA, Davydov A, Britanova OV et al (2016) High-quality full-length immunoglobulin profiling with unique molecular barcoding. Nat Protoc 11:1599\u20131616. https:\/\/doi.org\/10.1038\/nprot.2016.093","journal-title":"Nat Protoc"},{"key":"8_CR11","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1007\/978-1-61779-842-9_32","volume-title":"Immunogenetics: methods and applications in clinical practice","author":"E Alamyar","year":"2012","unstructured":"Alamyar E, Duroux P, Lefranc M-P, Giudicelli V (2012) IMGT\u00ae tools for the nucleotide analysis of immunoglobulin (IG) and T cell receptor (TR) V-(D)-J repertoires, polymorphisms, and IG mutations: IMGT\/V-QUEST and IMGT\/HighV-QUEST for NGS. In: Christiansen FT, Tait BD (eds) Immunogenetics: methods and applications in clinical practice. Humana Press, Totowa, pp 569\u2013604"},{"key":"8_CR12","doi-asserted-by":"publisher","first-page":"W34","DOI":"10.1093\/nar\/gkt382","volume":"41","author":"J Ye","year":"2013","unstructured":"Ye J, Ma N, Madden TL, Ostell JM (2013) IgBLAST: an immunoglobulin variable domain sequence analysis tool. Nucleic Acids Res 41:W34\u2013W40. https:\/\/doi.org\/10.1093\/nar\/gkt382","journal-title":"Nucleic Acids Res"}],"container-title":["Methods in Molecular Biology","RNA Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4726-4_8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T04:36:53Z","timestamp":1780807013000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4726-4_8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"ISBN":["9781071647257","9781071647264"],"references-count":12,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4726-4_8","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025]]},"assertion":[{"value":"24 July 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}