{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T08:15:18Z","timestamp":1778919318010,"version":"3.51.4"},"publisher-location":"New York, NY","reference-count":27,"publisher":"Springer US","isbn-type":[{"value":"9781071647370","type":"print"},{"value":"9781071647387","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-4738-7_6","type":"book-chapter","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T22:31:32Z","timestamp":1759271492000},"page":"91-104","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation for the Efficacy of Exon-Skipping Therapy Using In Vivo Assessment of Skeletal Muscle Function and Fragility"],"prefix":"10.1007","author":[{"given":"Yuki","family":"Ashida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsura","family":"Minegishi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Motohashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitsugu","family":"Aoki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,1]]},"reference":[{"key":"6_CR1","doi-asserted-by":"publisher","first-page":"1042","DOI":"10.1002\/jcsm.12347","volume":"9","author":"MF Jacques","year":"2018","unstructured":"Jacques MF, Onambele-Pearson GL, Reeves ND, Stebbings GK, Smith J, Morse CI (2018) Relationships between muscle size, strength, and physical activity in adults with muscular dystrophy. J Cachexia Sarcopenia Muscle 9:1042\u20131052. https:\/\/doi.org\/10.1002\/jcsm.12347","journal-title":"J Cachexia Sarcopenia Muscle"},{"key":"6_CR2","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1038\/mt.2010.186","volume":"18","author":"Y Aoki","year":"2010","unstructured":"Aoki Y, Nakamura A, Yokota T, Saito T, Okazawa H, Nagata T et al (2010) In-frame dystrophin following exon 51-skipping improves muscle pathology and function in the exon 52-deficient mdx mouse. Mol Ther 18:1995\u20132005. https:\/\/doi.org\/10.1038\/mt.2010.186","journal-title":"Mol Ther"},{"key":"6_CR3","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.937132","volume":"13","author":"EC Minchew","year":"2022","unstructured":"Minchew EC, Williamson NC, Readyoff AT, McClung JM, Spangenburg EE (2022) Isometric skeletal muscle contractile properties in common strains of male laboratory mice. Front Physiol 13:937132. https:\/\/doi.org\/10.3389\/fphys.2022.937132","journal-title":"Front Physiol"},{"key":"6_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00421-010-1480-0","volume":"110","author":"N Place","year":"2010","unstructured":"Place N, Yamada T, Bruton JD, Westerblad H (2010) Muscle fatigue: from observations in humans to underlying mechanisms studied in intact single muscle fibres. Eur J Appl Physiol 110:1\u201315","journal-title":"Eur J Appl Physiol"},{"key":"6_CR5","doi-asserted-by":"publisher","first-page":"C885","DOI":"10.1152\/ajpcell.00163.2022","volume":"323","author":"Y Ashida","year":"2022","unstructured":"Ashida Y, Himori K, Tokuda N, Naito A, Yamauchi N, Takenaka-Ninagawa N et al (2022) Dissociation of SH3 and cysteine-rich domain 3 and junctophilin 1 from dihydropyridine receptor in dystrophin-deficient muscles. Am J Physiol Cell Physiol 323:C885\u2013Cc95. https:\/\/doi.org\/10.1152\/ajpcell.00163.2022","journal-title":"Am J Physiol Cell Physiol"},{"key":"6_CR6","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/s0022-510x(99)00118-5","volume":"166","author":"JN Kornegay","year":"1999","unstructured":"Kornegay JN, Bogan DJ, Bogan JR, Childers MK, Cundiff DD, Petroski GF et al (1999) Contraction force generated by tarsal joint flexion and extension in dogs with golden retriever muscular dystrophy. J Neurol Sci 166:115\u2013121. https:\/\/doi.org\/10.1016\/s0022-510x(99)00118-5","journal-title":"J Neurol Sci"},{"key":"6_CR7","doi-asserted-by":"publisher","first-page":"5149","DOI":"10.1113\/jp270650","volume":"594","author":"AJ Cheng","year":"2016","unstructured":"Cheng AJ, Yamada T, Rassier DE, Andersson DC, Westerblad H, Lanner JT (2016) Reactive oxygen\/nitrogen species and contractile function in skeletal muscle during fatigue and recovery. J Physiol 594:5149\u20135160. https:\/\/doi.org\/10.1113\/jp270650","journal-title":"J Physiol"},{"key":"6_CR8","doi-asserted-by":"publisher","first-page":"5063","DOI":"10.1113\/jphysiol.2008.160507","volume":"586","author":"S Hollingworth","year":"2008","unstructured":"Hollingworth S, Zeiger U, Baylor SM (2008) Comparison of the myoplasmic calcium transient elicited by an action potential in intact fibres of mdx and normal mice. J Physiol 586:5063\u20135075. https:\/\/doi.org\/10.1113\/jphysiol.2008.160507","journal-title":"J Physiol"},{"key":"6_CR9","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1113\/jphysiol.1990.sp017916","volume":"420","author":"RH Fink","year":"1990","unstructured":"Fink RH, Stephenson DG, Williams DA (1990) Physiological properties of skinned fibres from normal and dystrophic (Duchenne) human muscle activated by Ca2+ and Sr2+. J Physiol 420:337\u2013353. https:\/\/doi.org\/10.1113\/jphysiol.1990.sp017916","journal-title":"J Physiol"},{"key":"6_CR10","doi-asserted-by":"publisher","first-page":"3710","DOI":"10.1073\/pnas.90.8.3710","volume":"90","author":"BJ Petrof","year":"1993","unstructured":"Petrof BJ, Shrager JB, Stedman HH, Kelly AM, Sweeney HL (1993) Dystrophin protects the sarcolemma from stresses developed during muscle contraction. Proc Natl Acad Sci USA 90:3710\u20133714","journal-title":"Proc Natl Acad Sci USA"},{"key":"6_CR11","doi-asserted-by":"publisher","first-page":"4225","DOI":"10.1093\/hmg\/ddv155","volume":"24","author":"C Godfrey","year":"2015","unstructured":"Godfrey C, Muses S, McClorey G, Wells KE, Coursindel T, Terry RL et al (2015) How much dystrophin is enough: the physiological consequences of different levels of dystrophin in the mdx mouse. Hum Mol Genet 24:4225\u20134237. https:\/\/doi.org\/10.1093\/hmg\/ddv155","journal-title":"Hum Mol Genet"},{"key":"6_CR12","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213208","volume":"155","author":"L Kiriaev","year":"2023","unstructured":"Kiriaev L, Baumann CW, Lindsay A (2023) Eccentric contraction-induced strength loss in dystrophin-deficient muscle: preparations, protocols, and mechanisms. J Gen Physiol 155:e202213208. https:\/\/doi.org\/10.1085\/jgp.202213208","journal-title":"J Gen Physiol"},{"key":"6_CR13","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1113\/jphysiol.2012.241679","volume":"591","author":"SJP Pratt","year":"2013","unstructured":"Pratt SJP, Shah SB, Ward CW, Inacio MP, Stains JP, Lovering RM (2013) Effects of in vivo injury on the neuromuscular junction in healthy and dystrophic muscles. J Physiol 591:559\u2013570. https:\/\/doi.org\/10.1113\/jphysiol.2012.241679","journal-title":"J Physiol"},{"key":"6_CR14","doi-asserted-by":"publisher","first-page":"3765","DOI":"10.1113\/jphysiol.2013.254656","volume":"591","author":"JA Call","year":"2013","unstructured":"Call JA, Warren GL, Verma M, Lowe DA (2013) Acute failure of action potential conduction in mdx muscle reveals new mechanism of contraction-induced force loss. J Physiol 591:3765\u20133776. https:\/\/doi.org\/10.1113\/jphysiol.2013.254656","journal-title":"J Physiol"},{"issue":"131","key":"6_CR15","doi-asserted-by":"publisher","first-page":"1399","DOI":"10.1152\/japplphysiol.00463.2021","volume":"2021","author":"Y Ashida","year":"1985","unstructured":"Ashida Y, Himori K, Tamai K, Kimura I, Yamada T (1985) Preconditioning contractions prevent prolonged force depression and ca(2+)-dependent proteolysis of STAC3 after damaging eccentric contractions. J Appl Physiol 2021(131):1399\u20131407. https:\/\/doi.org\/10.1152\/japplphysiol.00463.2021","journal-title":"J Appl Physiol"},{"key":"6_CR16","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1249\/00005768-199205000-00004","volume":"24","author":"PM Clarkson","year":"1992","unstructured":"Clarkson PM, Nosaka K, Braun B (1992) Muscle function after exercise-induced muscle damage and rapid adaptation. Med Sci Sports Exerc 24:512\u2013520","journal-title":"Med Sci Sports Exerc"},{"issue":"108","key":"6_CR17","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1152\/japplphysiol.00803.2009","volume":"2010","author":"B Blaauw","year":"1985","unstructured":"Blaauw B, Agatea L, Toniolo L, Canato M, Quarta M, Dyar KA et al (1985) Eccentric contractions lead to myofibrillar dysfunction in muscular dystrophy. J Appl Physiol 2010(108):105\u2013111. https:\/\/doi.org\/10.1152\/japplphysiol.00803.2009","journal-title":"J Appl Physiol"},{"key":"6_CR18","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1006\/bbrc.1997.7328","volume":"238","author":"E Araki","year":"1997","unstructured":"Araki E, Nakamura K, Nakao K, Kameya S, Kobayashi O, Nonaka I et al (1997) Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observed in Duchenne muscular dystrophy. Biochem Biophys Res Commun 238:492\u2013497. https:\/\/doi.org\/10.1006\/bbrc.1997.7328","journal-title":"Biochem Biophys Res Commun"},{"key":"6_CR19","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1538\/expanim.52.93","volume":"52","author":"Y Shimatsu","year":"2003","unstructured":"Shimatsu Y, Katagiri K, Furuta T, Nakura M, Tanioka Y, Yuasa K et al (2003) Canine X-linked muscular dystrophy in Japan (CXMDJ). Exp Anim 52:93\u201397. https:\/\/doi.org\/10.1538\/expanim.52.93","journal-title":"Exp Anim"},{"key":"6_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1471-2474-9-1","volume":"9","author":"K Yuasa","year":"2008","unstructured":"Yuasa K, Nakamura A, Hijikata T, Takeda S (2008) Dystrophin deficiency in canine X-linked muscular dystrophy in Japan (CXMDJ) alters myosin heavy chain expression profiles in the diaphragm more markedly than in the tibialis cranialis muscle. BMC Musculoskelet Disord 9:1. https:\/\/doi.org\/10.1186\/1471-2474-9-1","journal-title":"BMC Musculoskelet Disord"},{"key":"6_CR21","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1002\/1097-4598(200103)24:3<387::aid-mus1010>3.0.co;2-m","volume":"24","author":"CM Gregory","year":"2001","unstructured":"Gregory CM, Vandenborne K, Dudley GA (2001) Metabolic enzymes and phenotypic expression among human locomotor muscles. Muscle Nerve 24:387\u2013393. https:\/\/doi.org\/10.1002\/1097-4598(200103)24:3<387::aid-mus1010>3.0.co;2-m","journal-title":"Muscle Nerve"},{"key":"6_CR22","doi-asserted-by":"publisher","first-page":"2562","DOI":"10.1111\/febs.14831","volume":"286","author":"A Lindsay","year":"2019","unstructured":"Lindsay A, Southern WM, McCourt PM, Larson AA, Hodges JS, Lowe DA et al (2019) Variable cytoplasmic actin expression impacts the sensitivity of different dystrophin-deficient mdx skeletal muscles to eccentric contraction. FEBS J 286:2562\u20132576. https:\/\/doi.org\/10.1111\/febs.14831","journal-title":"FEBS J"},{"key":"6_CR23","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101204R","volume":"35","author":"T Yamada","year":"2021","unstructured":"Yamada T, Kimura I, Ashida Y, Tamai K, Fusagawa H, Tohse N et al (2021) Larger improvements in fatigue resistance and mitochondrial function with high- than with low-intensity contractions during interval training of mouse skeletal muscle. FASEB J 35:e21988. https:\/\/doi.org\/10.1096\/fj.202101204R","journal-title":"FASEB J"},{"key":"6_CR24","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1186\/s13395-016-0085-7","volume":"6","author":"JN Kornegay","year":"2016","unstructured":"Kornegay JN, Bogan DJ, Bogan JR, Dow JL, Wang J, Fan Z et al (2016) Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures. Skelet Muscle 6:14. https:\/\/doi.org\/10.1186\/s13395-016-0085-7","journal-title":"Skelet Muscle"},{"key":"6_CR25","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/978-1-61737-982-6_7","volume":"709","author":"JN Kornegay","year":"2011","unstructured":"Kornegay JN, Bogan JR, Bogan DJ, Childers MK, Grange RW (2011) Golden retriever muscular dystrophy (GRMD): developing and maintaining a colony and physiological functional measurements. Methods Mol Biol 709:105\u2013123. https:\/\/doi.org\/10.1007\/978-1-61737-982-6_7","journal-title":"Methods Mol Biol"},{"issue":"102","key":"6_CR26","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1152\/japplphysiol.00776.2006","volume":"2007","author":"T Garma","year":"1985","unstructured":"Garma T, Kobayashi C, Haddad F, Adams GR, Bodell PW, Baldwin KM (1985) Similar acute molecular responses to equivalent volumes of isometric, lengthening, or shortening mode resistance exercise. J Appl Physiol 2007(102):135\u2013143. https:\/\/doi.org\/10.1152\/japplphysiol.00776.2006","journal-title":"J Appl Physiol"},{"key":"6_CR27","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.1007\/s00421-015-3124-x","volume":"115","author":"D Neyroud","year":"2015","unstructured":"Neyroud D, Temesi J, Millet GY, Verges S, Maffiuletti NA, Kayser B et al (2015) Comparison of electrical nerve stimulation, electrical muscle stimulation and magnetic nerve stimulation to assess the neuromuscular function of the plantar flexor muscles. Eur J Appl Physiol 115:1429\u20131439. https:\/\/doi.org\/10.1007\/s00421-015-3124-x","journal-title":"Eur J Appl Physiol"}],"container-title":["Methods in Molecular Biology","Splice Switching"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4738-7_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T07:58:34Z","timestamp":1778918314000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4738-7_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,1]]},"ISBN":["9781071647370","9781071647387"],"references-count":27,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4738-7_6","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,1]]},"assertion":[{"value":"1 October 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}