{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T05:05:05Z","timestamp":1774933505129,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":16,"publisher":"Springer US","isbn-type":[{"value":"9781071647943","type":"print"},{"value":"9781071647950","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-4795-0_6","type":"book-chapter","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T22:18:35Z","timestamp":1759270715000},"page":"55-65","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Dissection Procedures for EGC Isolation by Human Mucosal Biopsies"],"prefix":"10.1007","author":[{"given":"Giovanni","family":"Sarnelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Palenca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Sole","family":"Facioni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierluigi","family":"De Pierro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcella","family":"Pesce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,10,1]]},"reference":[{"issue":"4","key":"6_CR1","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1002\/cne.21769","volume":"509","author":"S Hoff","year":"2008","unstructured":"Hoff S, Zeller F, von Weyhern CW et al (2008) Quantitative assessment of glial cells in the human and Guinea pig enteric nervous system with an anti-Sox8\/9\/10 antibody. J Comp Neurol 509(4):356\u2013371. https:\/\/doi.org\/10.1002\/cne.21769","journal-title":"J Comp Neurol"},{"issue":"10","key":"6_CR2","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.3748\/wjg.v17.i10.1261","volume":"17","author":"C Cirillo","year":"2011","unstructured":"Cirillo C, Sarnelli G, Esposito G et al (2011) S100B protein in the gut: the evidence for enteroglial-sustained intestinal inflammation. World J Gastroenterol 17(10):1261\u20131266. https:\/\/doi.org\/10.3748\/wjg.v17.i10.1261","journal-title":"World J Gastroenterol"},{"issue":"11","key":"6_CR3","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1038\/nrgastro.2012.138","volume":"9","author":"BD Gulbransen","year":"2019","unstructured":"Gulbransen BD, Sharkey KA (2019) Novel functional roles for enteric glia in the gastrointestinal tract. Nat Rev Gastroenterol Hepatol 9(11):625\u2013632. https:\/\/doi.org\/10.1038\/nrgastro.2012.138","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"2","key":"6_CR4","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1038\/nrgastro.2012.221","volume":"10","author":"M Neunlist","year":"2013","unstructured":"Neunlist M, Van Landeghem L, Mah\u00e9 MM et al (2013) The digestive neuronal-glial-epithelial unit: a new actor in gut health and disease. Nat Rev Gastroenterol Hepatol 10(2):90\u2013100. https:\/\/doi.org\/10.1038\/nrgastro.2012.221","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"2","key":"6_CR5","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1002\/glia.22746","volume":"63","author":"W Boesmans","year":"2015","unstructured":"Boesmans W, Lasrado R, Vanden Berghe P et al (2015) (2015) heterogeneity and phenotypic plasticity of glial cells in the mammalian enteric nervous system. Glia 63(2):229\u2013241. https:\/\/doi.org\/10.1002\/glia.22746","journal-title":"Glia"},{"key":"6_CR6","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1080\/19490976.2015.1109767","volume":"6","author":"PS Kabouridis","year":"2015","unstructured":"Kabouridis PS, Lasrado R, McCallum S et al (2015) The gut microbiota keeps enteric glial cells on the move; prospective roles of the gut epithelium and immune system. Gut Microbes 6:398\u2013403. https:\/\/doi.org\/10.1080\/19490976.2015.1109767","journal-title":"Gut Microbes"},{"issue":"9","key":"6_CR7","doi-asserted-by":"publisher","first-page":"e372","DOI":"10.1111\/j.1365-2982.2011.01748.x","volume":"23","author":"C Cirillo","year":"2011","unstructured":"Cirillo C, Sarnelli G, Turco F et al (2011) Proinflammatory stimuli activates human-derived enteroglial cells and induces autocrine nitric oxide production. Neurogastroenterol Motil 23(9):e372\u2013e382. https:\/\/doi.org\/10.1111\/j.1365-2982.2011.01748.x","journal-title":"Neurogastroenterol Motil"},{"key":"6_CR8","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1038\/s41575-021-00423-7","volume":"18","author":"L Seguella","year":"2021","unstructured":"Seguella L, Gulbransen BD (2021) Enteric glial biology, intercellular signalling and roles in gastrointestinal disease. Nat Rev Gastroenterol Hepatol 18:571\u2013587. https:\/\/doi.org\/10.1038\/s41575-021-00423-7","journal-title":"Nat Rev Gastroenterol Hepatol"},{"issue":"2","key":"6_CR9","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1002\/cyto.a.23690","volume":"95","author":"A Reichard","year":"2019","unstructured":"Reichard A, Asosingh K (2019) Best practices for preparing a single cell suspension from solid tissues for flow cytometry. Cytometry A 95(2):219\u2013226. https:\/\/doi.org\/10.1002\/cyto.a.23690","journal-title":"Cytometry A"},{"issue":"1","key":"6_CR10","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1136\/gutjnl-2012-302090","volume":"63","author":"F Turco","year":"2014","unstructured":"Turco F, Sarnelli G, Cirillo C et al (2014) Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut 63(1):105\u2013115. https:\/\/doi.org\/10.1136\/gutjnl-2012-302090","journal-title":"Gut"},{"issue":"18","key":"6_CR11","doi-asserted-by":"publisher","first-page":"e4825","DOI":"10.21769\/BioProtoc.4825","volume":"13","author":"C Morral","year":"2023","unstructured":"Morral C, Ghinnagow R, Karakasheva T et al (2023) Isolation of epithelial and stromal cells from colon tissues in homeostasis and under inflammatory conditions. Bio Protoc 13(18):e4825. https:\/\/doi.org\/10.21769\/BioProtoc.4825","journal-title":"Bio Protoc"},{"issue":"2","key":"6_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2024.103057","volume":"5","author":"ML Caldwell","year":"2024","unstructured":"Caldwell ML, Cook CA, Mariant CL, Touvron M, Odle J, Blikslager AT, Ziegler AL, Van Landeghem L (2024) Protocol to enteric glial cells from the submucosal and myenteric plexi of neonatal and juvenile pig colons. STAR Protoc 5(2):103057. https:\/\/doi.org\/10.1016\/j.xpro.2024.103057","journal-title":"STAR Protoc"},{"key":"6_CR13","doi-asserted-by":"publisher","DOI":"10.1002\/9780470649367","volume-title":"Culture of animal cells: a manual of basic technique and specialized applications","author":"RI Freshney","year":"2010","unstructured":"Freshney RI (2010) Culture of animal cells: a manual of basic technique and specialized applications, 6th edn. Wiley","edition":"6"},{"key":"6_CR14","volume-title":"Molecular cloning: a laboratory manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press","edition":"2"},{"issue":"1","key":"6_CR15","first-page":"25","volume":"85","author":"N Thosani","year":"2017","unstructured":"ASGE Technology Committee, Thosani N (2017) Endoscopic mucosal tissue sampling. Gastrointest Endosc 85(1):25\u201333","journal-title":"Gastrointest Endosc"},{"issue":"4","key":"6_CR16","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.gie.2011.07.025","volume":"74","author":"DA Fisher","year":"2016","unstructured":"ASGE Standards of Practice Committee, Fisher DA, Maple JT, Ben-Menachem T et al (2016) Complications of colonoscopy. Gastrointest Endosc 74(4):745\u2013752","journal-title":"Gastrointest Endosc"}],"container-title":["Methods in Molecular Biology","Enteric Glial Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4795-0_6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T03:29:41Z","timestamp":1774927781000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4795-0_6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,1]]},"ISBN":["9781071647943","9781071647950"],"references-count":16,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4795-0_6","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,1]]},"assertion":[{"value":"1 October 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}