{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T23:47:30Z","timestamp":1775260050685,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":7,"publisher":"Springer US","isbn-type":[{"value":"9781071648476","type":"print"},{"value":"9781071648483","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-4848-3_15","type":"book-chapter","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T10:14:07Z","timestamp":1762164847000},"page":"237-250","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identifying Trypanosoma cruzi Proteins Targeted by Chagas Disease Patient Antibodies Using Yeast Surface Display"],"prefix":"10.1007","author":[{"given":"Mira","family":"Loock","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor","family":"Cestari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"15_CR1","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1007\/978-1-4939-2748-7_8","volume":"1319","author":"GM Cherf","year":"2015","unstructured":"Cherf GM, Cochran JR (2015) Applications of yeast surface display for protein engineering. Methods Mol Biol 1319:155\u2013175. https:\/\/doi.org\/10.1007\/978-1-4939-2748-7_8","journal-title":"Methods Mol Biol"},{"key":"15_CR2","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.794742","volume":"9","author":"KV Teymennet-Ram\u00edrez","year":"2021","unstructured":"Teymennet-Ram\u00edrez KV, Mart\u00ednez-Morales F, Trejo-Hern\u00e1ndez MR (2021) Yeast surface display system: strategies for improvement and biotechnological applications. Front Bioeng Biotechnol 9:794742. https:\/\/doi.org\/10.3389\/fbioe.2021.794742","journal-title":"Front Bioeng Biotechnol"},{"issue":"5","key":"15_CR3","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1021\/acsinfecdis.2c00568","volume":"9","author":"R Heslop","year":"2023","unstructured":"Heslop R et al (2023) Genome-wide libraries for protozoan pathogen drug target screening using yeast surface display. ACS Infect Dis 9(5):1078\u20131091. https:\/\/doi.org\/10.1021\/acsinfecdis.2c00568","journal-title":"ACS Infect Dis"},{"key":"15_CR4","doi-asserted-by":"publisher","unstructured":"Sternlieb T, Loock M, Gao M, Cestari I (2022) Efficient Generation of Genome-wide Libraries for Protein\u2013ligand Screens Using Gibson Assembly. Bio-protocol 12(22). https:\/\/doi.org\/10.21769\/BioProtoc.4558","DOI":"10.21769\/BioProtoc.4558"},{"issue":"2","key":"15_CR5","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1002\/cyto.990110203","volume":"11","author":"S Miltenyi","year":"1990","unstructured":"Miltenyi S, M\u00fcller W, Weichel W, Radbruch A (1990) High gradient magnetic cell separation with MACS. Cytometry 11(2):231\u2013238. https:\/\/doi.org\/10.1002\/cyto.990110203","journal-title":"Cytometry"},{"key":"15_CR6","doi-asserted-by":"publisher","unstructured":"Loock M et al (2023) High-efficiency transformation and expression of genomic libraries in yeast. Methods Protoc 6(5). https:\/\/doi.org\/10.3390\/mps6050089","DOI":"10.3390\/mps6050089"},{"key":"15_CR7","volume-title":"Molecular cloning: a laboratory manual","author":"JRD Sambrook","year":"2001","unstructured":"Sambrook JRD (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY"}],"container-title":["Methods in Molecular Biology","T. cruzi Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4848-3_15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T22:29:35Z","timestamp":1775255375000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4848-3_15"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"ISBN":["9781071648476","9781071648483"],"references-count":7,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4848-3_15","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"4 November 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}