{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T23:48:19Z","timestamp":1775260099379,"version":"3.50.1"},"publisher-location":"New York, NY","reference-count":12,"publisher":"Springer US","isbn-type":[{"value":"9781071648476","type":"print"},{"value":"9781071648483","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-4848-3_24","type":"book-chapter","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T10:14:17Z","timestamp":1762164857000},"page":"363-377","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrastructure Expansion Microscopy for the Analysis of Trypanosoma cruzi Infection"],"prefix":"10.1007","author":[{"given":"Felipe","family":"Castro Capurro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria Lucia","family":"Alonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,4]]},"reference":[{"key":"24_CR1","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1007\/s13534-024-00365-4","volume":"14","author":"M Shin","year":"2024","unstructured":"Shin M, Seo M, Lee K et al (2024) Super-resolution techniques for biomedical applications and challenges. Biomed Eng Lett 14:465\u2013496","journal-title":"Biomed Eng Lett"},{"key":"24_CR2","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1146\/annurev-cellbio-100818-125320","volume":"35","author":"PW Tillberg","year":"2019","unstructured":"Tillberg PW, Chen F (2019) Expansion microscopy: scalable and convenient super-resolution microscopy. Annu Rev Cell Dev Biol 35:683\u2013701","journal-title":"Annu Rev Cell Dev Biol"},{"key":"24_CR3","doi-asserted-by":"publisher","first-page":"3299","DOI":"10.1021\/acs.chemrev.2c00711","volume":"123","author":"G Wen","year":"2022","unstructured":"Wen G, Leen V, Rohand T et al (2022) Current progress in expansion microscopy: chemical strategies and applications. Chem Rev 123:3299\u20133323","journal-title":"Chem Rev"},{"key":"24_CR4","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/s41592-018-0238-1","volume":"16","author":"D Gambarotto","year":"2018","unstructured":"Gambarotto D, Zwettler FU, Le Guennec M et al (2018) Imaging cellular ultrastructures using expansion microscopy (U-ExM). Nat Methods 16:71\u201374","journal-title":"Nat Methods"},{"key":"24_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00436-022-07619-z","volume":"121","author":"VL Alonso","year":"2022","unstructured":"Alonso VL (2022) Ultrastructure expansion microscopy (U-ExM) in Trypanosoma cruzi: localization of tubulin isoforms and isotypes. Parasitol Res 121:1\u20136","journal-title":"Parasitol Res"},{"key":"24_CR6","doi-asserted-by":"crossref","unstructured":"Kalichava A, Ochsenreiter T (2021) Ultrastructure expansion microscopy in Trypanosoma brucei. Open Biol 11","DOI":"10.1098\/rsob.210132"},{"key":"24_CR7","volume-title":"Cytoskeleton \u2013 structure, dynamics, function and disease","author":"JC Vidal","year":"2017","unstructured":"Vidal JC, de Souza W (2017) Morphological and functional aspects of cytoskeleton of trypanosomatids. In: Cytoskeleton \u2013 structure, dynamics, function and disease. InTech"},{"key":"24_CR8","doi-asserted-by":"publisher","first-page":"12677","DOI":"10.1021\/acsnano.7b07200","volume":"11","author":"AR Halpern","year":"2017","unstructured":"Halpern AR, Alas GCM, Chozinski TJ et al (2017) Hybrid structured illumination expansion microscopy reveals microbial cytoskeleton organization. ACS Nano 11:12677\u201312686","journal-title":"ACS Nano"},{"key":"24_CR9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001020","volume":"19","author":"E Bertiaux","year":"2021","unstructured":"Bertiaux E, Balestra AC, Bournonville L et al (2021) Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid. PLoS Biol 19:e3001020","journal-title":"PLoS Biol"},{"key":"24_CR10","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/978-1-0716-1681-9_8","volume":"2369","author":"PN Dos Santos","year":"2021","unstructured":"Dos Santos PN, Soldati-Favre D (2021) Coupling auxin-inducible degron system with ultrastructure expansion microscopy to accelerate the discovery of gene function in Toxoplasma gondii. Methods Mol Biol 2369:121\u2013137","journal-title":"Methods Mol Biol"},{"key":"24_CR11","volume":"12","author":"MA de Hern\u00e1ndez","year":"2023","unstructured":"de Hern\u00e1ndez MA, Peralta GM, Vena R et al (2023) Ultrastructural expansion microscopy in three in vitro life cycle stages of Trypanosoma cruzi. J Vis Exp 12:e65381","journal-title":"J Vis Exp"},{"key":"24_CR12","doi-asserted-by":"publisher","first-page":"866","DOI":"10.3390\/pathogens13100866","volume":"13","author":"R Tomasina","year":"2024","unstructured":"Tomasina R, Gonz\u00e1lez FC, Cabrera A et al (2024) From trypomastigotes to trypomastigotes: analyzing the one-way intracellular journey of Trypanosoma cruzi by ultrastructure expansion microscopy. Pathogens 13:866","journal-title":"Pathogens"}],"container-title":["Methods in Molecular Biology","T. cruzi Infection"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4848-3_24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T22:30:22Z","timestamp":1775255422000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4848-3_24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"ISBN":["9781071648476","9781071648483"],"references-count":12,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4848-3_24","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"4 November 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}