{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:56:24Z","timestamp":1781265384482,"version":"3.54.1"},"publisher-location":"New York, NY","reference-count":39,"publisher":"Springer US","isbn-type":[{"value":"9781071649961","type":"print"},{"value":"9781071649978","type":"electronic"}],"license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026]]},"DOI":"10.1007\/978-1-0716-4997-8_5","type":"book-chapter","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T09:36:17Z","timestamp":1763717777000},"page":"63-78","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Macrophage Cell Culture and Characterization by Serum Human AB in Health and Disease"],"prefix":"10.1007","author":[{"given":"Audrey","family":"Paoletti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bineta","family":"Ly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Bitoun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetane","family":"Nocturne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Mariette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,22]]},"reference":[{"issue":"3","key":"5_CR1","doi-asserted-by":"publisher","first-page":"204","DOI":"10.1159\/000296507","volume":"2","author":"A Parihar","year":"2010","unstructured":"Parihar A, Eubank TD, Doseff AI (2010) Monocytes and macrophages regulate immunity through dynamic networks of survival and cell death. J Innate Immun 2(3):204\u2013215","journal-title":"J Innate Immun"},{"issue":"7","key":"5_CR2","doi-asserted-by":"publisher","first-page":"2413","DOI":"10.2741\/3387","volume":"14","author":"ME Gonzalez-Mejia","year":"2009","unstructured":"Gonzalez-Mejia ME, Doseff AI (2009) Regulation of monocytes and macrophages cell fate. Front Biosci 14(7):2413\u20132431","journal-title":"Front Biosci"},{"issue":"1","key":"5_CR3","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1189\/jlb.69.1.11","volume":"69","author":"E Grage-Griebenow","year":"2001","unstructured":"Grage-Griebenow E, Flad HD, Ernst M (2001) Heterogeneity of human peripheral blood monocyte subsets. J Leukoc Biol 69(1):11\u201320","journal-title":"J Leukoc Biol"},{"issue":"3","key":"5_CR4","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1189\/jlb.0806510","volume":"81","author":"L Ziegler-Heitbrock","year":"2007","unstructured":"Ziegler-Heitbrock L (2007) The CD14+ CD16+ blood monocytes: their role in infection and inflammation. J Leukoc Biol 81(3):584\u2013592","journal-title":"J Leukoc Biol"},{"issue":"1","key":"5_CR5","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S1074-7613(03)00174-2","volume":"19","author":"F Geissmann","year":"2003","unstructured":"Geissmann F, Jung S, Littman DR (2003) Blood monocytes consist of two principal subsets with distinct migratory properties. Immunity 19(1):71\u201382","journal-title":"Immunity"},{"issue":"5966","key":"5_CR6","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1126\/science.1178331","volume":"327","author":"F Geissmann","year":"2010","unstructured":"Geissmann F, Manz MG, Jung S, Sieweke MH, Merad M, Ley K (2010) Development of monocytes, macrophages, and dendritic cells. Science 327(5966):656\u2013661","journal-title":"Science"},{"issue":"12","key":"5_CR7","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1038\/nri1733","volume":"5","author":"S Gordon","year":"2005","unstructured":"Gordon S, Taylor PR (2005) Monocyte and macrophage heterogeneity. Nat Rev Immunol 5(12):953\u2013964","journal-title":"Nat Rev Immunol"},{"issue":"2","key":"5_CR8","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1007\/s40828-022-00163-4","volume":"8","author":"U Lendeckel","year":"2022","unstructured":"Lendeckel U, Venz S, Wolke C (2022) Macrophages: shapes and functions. ChemTexts 8(2):12","journal-title":"ChemTexts"},{"issue":"11","key":"5_CR9","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.620339","volume":"19","author":"GR Pidwill","year":"2021","unstructured":"Pidwill GR, Gibson JF, Cole J, Renshaw SA, Foster SJ (2021) The role of macrophages in Staphylococcus aureus infection. Front Immunol 19(11):620339","journal-title":"Front Immunol"},{"key":"5_CR10","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1146\/annurev.immunol.23.021704.115816","volume":"23","author":"PR Taylor","year":"2005","unstructured":"Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S (2005) Macrophage receptors and immune recognition. Annu Rev Immunol 23:901\u2013944","journal-title":"Annu Rev Immunol"},{"issue":"2","key":"5_CR11","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/s41577-023-00921-6","volume":"24","author":"GC Brown","year":"2024","unstructured":"Brown GC (2024) Cell death by phagocytosis. Nat Rev Immunol 24(2):91\u2013102","journal-title":"Nat Rev Immunol"},{"issue":"8","key":"5_CR12","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e29686","volume":"10","author":"SP Dash","year":"2024","unstructured":"Dash SP, Gupta S, Sarangi PP (2024) Monocytes and macrophages: origin, homing, differentiation, and functionality during inflammation. Heliyon 10(8):e29686","journal-title":"Heliyon"},{"issue":"877","key":"5_CR13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173090","volume":"15","author":"C Yunna","year":"2020","unstructured":"Yunna C, Mengru H, Lei W, Weidong C (2020) Macrophage M1\/M2 polarization. Eur J Pharmacol 15(877):173090","journal-title":"Eur J Pharmacol"},{"issue":"10","key":"5_CR14","doi-asserted-by":"publisher","first-page":"1084","DOI":"10.3389\/fimmu.2019.01084","volume":"24","author":"M Orecchioni","year":"2019","unstructured":"Orecchioni M, Ghosheh Y, Pramod AB, Ley K (2019) Macrophage polarization: different gene signatures in M1(LPS+) vs. classically and M2(LPS-) vs. alternatively activated macrophages. Front Immunol 24(10):1084","journal-title":"Front Immunol"},{"issue":"9","key":"5_CR15","doi-asserted-by":"publisher","first-page":"6425","DOI":"10.1002\/jcp.26429","volume":"233","author":"A Shapouri-Moghaddam","year":"2018","unstructured":"Shapouri-Moghaddam A, Mohammadian S, Vazini H, Taghadosi M, Esmaeili SA, Mardani F, Seifi B, Mohammadi A, Afshari JT, Sahebkar A (2018) Macrophage plasticity, polarization, and function in health and disease. J Cell Physiol 233(9):6425\u20136440","journal-title":"J Cell Physiol"},{"issue":"15","key":"5_CR16","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1146\/annurev-pathmechdis-012418-012718","volume":"24","author":"M Locati","year":"2020","unstructured":"Locati M, Curtale G, Mantovani A (2020) Diversity, mechanisms, and significance of macrophage plasticity. Annu Rev Pathol 24(15):123\u2013147","journal-title":"Annu Rev Pathol"},{"issue":"2","key":"5_CR17","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/imm.12910","volume":"154","author":"SC Funes","year":"2018","unstructured":"Funes SC, Rios M, Escobar-Vera J, Kalergis AM (2018) Implications of macrophage polarization in autoimmunity. Immunology 154(2):186\u2013195","journal-title":"Immunology"},{"issue":"12","key":"5_CR18","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1038\/nrd.2018.169","volume":"17","author":"L Cassetta","year":"2018","unstructured":"Cassetta L, Pollard JW (2018) Targeting macrophages: therapeutic approaches in cancer. Nat Rev Drug Discov 17(12):887\u2013904","journal-title":"Nat Rev Drug Discov"},{"issue":"1","key":"5_CR19","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1038\/nri978","volume":"3","author":"S Gordon","year":"2003","unstructured":"Gordon S (2003) Alternative activation of macrophages. Nat Rev Immunol 3(1):23\u201335","journal-title":"Nat Rev Immunol"},{"key":"5_CR20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2019.154939","volume":"127","author":"E Trus","year":"2020","unstructured":"Trus E, Basta S, Gee K (2020) Who\u2019s in charge here? Macrophage colony stimulating factor and granulocyte macrophage colony stimulating factor: competing factors in macrophage polarization. Cytokine 127:154939","journal-title":"Cytokine"},{"issue":"5","key":"5_CR21","first-page":"554","volume":"21","author":"TA Hamilton","year":"2014","unstructured":"Hamilton TA, Zhao C, Pavicic PG Jr, Datta S (2014) Myeloid colony-stimulating factors as regulators of macrophage polarization. Front Immunol 21(5):554","journal-title":"Front Immunol"},{"issue":"42","key":"5_CR22","doi-asserted-by":"publisher","first-page":"E3997","DOI":"10.1073\/pnas.1306719110","volume":"110","author":"A Allouch","year":"2013","unstructured":"Allouch A, David A, Amie SM, Lahouassa H, Chartier L, Margottin-Goguet F, Barr\u00e9-Sinoussi F, Kim B, S\u00e1ez-Ciri\u00f3n A, Pancino G (2013) p21-mediated RNR2 repression restricts HIV-1 replication in macrophages by inhibiting dNTP biosynthesis pathway. Proc Natl Acad Sci USA 110(42):E3997\u2013E4006","journal-title":"Proc Natl Acad Sci USA"},{"issue":"13","key":"5_CR23","doi-asserted-by":"publisher","first-page":"3381","DOI":"10.1016\/j.celrep.2019.02.095","volume":"28","author":"A Paoletti","year":"2019","unstructured":"Paoletti A, Allouch A, Caillet M, Sa\u00efdi H, Subra F, Nardacci R, Wu Q, Muradova Z, Voisin L, Raza SQ, Law F, Thoreau M, Dakhli H, Delelis O, Poirier-Beaudouin B, Dereuddre-Bosquet N, Le Grand R, Lambotte O, Saez-Cirion A, Pancino G, Ojcius DM, Solary E, Deutsch E, Piacentini M, Gougeon ML, Kroemer G, Perfettini JL (2019) HIV-1 envelope overcomes NLRP3-mediated inhibition of F-actin polymerization for viral entry. Cell Rep 28(13):3381\u20133394.e7","journal-title":"Cell Rep"},{"issue":"12","key":"5_CR24","doi-asserted-by":"publisher","first-page":"3243","DOI":"10.1038\/s41418-020-0573-5","volume":"27","author":"A Allouch","year":"2020","unstructured":"Allouch A, Di Primio C, Paoletti A, L\u00ea-Bury G, Subra F, Quercioli V, Nardacci R, David A, Sa\u00efdi H, Cereseto A, Ojcius DM, Montagnac G, Niedergang F, Pancino G, Saez-Cirion A, Piacentini M, Gougeon ML, Kroemer G, Perfettini JL (2020) SUGT1 controls susceptibility to HIV-1 infection by stabilizing microtubule plus-ends. Cell Death Differ 27(12):3243\u20133257","journal-title":"Cell Death Differ"},{"issue":"13","key":"5_CR25","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.832117","volume":"23","author":"A Paoletti","year":"2022","unstructured":"Paoletti A, Ly B, Bitoun S, Nocturne G, Rivi\u00e8re E, Manson JJ, Matucci A, Pallardy M, De Vries N, Mariette X (2022) Restoration of default blood monocyte-derived macrophage polarization with adalimumab but not Etanercept in rheumatoid arthritis. Front Immunol 23(13):832117","journal-title":"Front Immunol"},{"issue":"7","key":"5_CR26","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.4049\/jimmunol.1900386","volume":"203","author":"A Paoletti","year":"2019","unstructured":"Paoletti A, Rohmer J, Ly B, Pascaud J, Rivi\u00e8re E, Seror R, Le Goff B, Nocturne G, Mariette X (2019) Monocyte\/macrophage abnormalities specific to rheumatoid arthritis are linked to miR-155 and are differentially modulated by different TNF inhibitors. J Immunol 203(7):1766\u20131775","journal-title":"J Immunol"},{"issue":"10086","key":"5_CR27","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1016\/S0140-6736(17)31472-1","volume":"389","author":"IB McInnes","year":"2017","unstructured":"McInnes IB, Schett G (2017) Pathogenetic insights from the treatment of rheumatoid arthritis. Lancet 389(10086):2328\u20132337","journal-title":"Lancet"},{"issue":"5","key":"5_CR28","doi-asserted-by":"publisher","first-page":"472","DOI":"10.1007\/s11926-012-0275-1","volume":"14","author":"MK Demoruelle","year":"2012","unstructured":"Demoruelle MK, Deane KD (2012) Treatment strategies in early rheumatoid arthritis and prevention of rheumatoid arthritis. Curr Rheumatol Rep 14(5):472\u2013480","journal-title":"Curr Rheumatol Rep"},{"issue":"13","key":"5_CR29","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1001\/jama.2018.13103","volume":"320","author":"D Aletaha","year":"2018","unstructured":"Aletaha D, Smolen JS (2018) Diagnosis and management of rheumatoid arthritis: a review. JAMA 320(13):1360\u20131372","journal-title":"JAMA"},{"issue":"10086","key":"5_CR30","doi-asserted-by":"publisher","first-page":"2338","DOI":"10.1016\/S0140-6736(17)31491-5","volume":"389","author":"GR Burmester","year":"2017","unstructured":"Burmester GR, Pope JE (2017) Novel treatment strategies in rheumatoid arthritis. Lancet 389(10086):2338\u20132348","journal-title":"Lancet"},{"key":"5_CR31","doi-asserted-by":"publisher","first-page":"100","DOI":"10.2174\/1874312901105010100","volume":"5","author":"MD Smith","year":"2011","unstructured":"Smith MD (2011) The normal synovium. Open Rheumatol J 5:100\u2013106","journal-title":"Open Rheumatol J"},{"issue":"2020","key":"5_CR32","first-page":"1583647","volume":"13","author":"J Tu","year":"2020","unstructured":"Tu J, Wang X, Gong X, Hong W, Han D, Fang Y, Guo Y, Wei W (2020) Synovial macrophages in rheumatoid arthritis: the past, present, and future. Mediat Inflamm 13(2020):1583647","journal-title":"Mediat Inflamm"},{"issue":"8","key":"5_CR33","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1038\/s41591-020-0939-8","volume":"26","author":"S Alivernini","year":"2020","unstructured":"Alivernini S, MacDonald L, Elmesmari A, Finlay S, Tolusso B, Gigante MR, Petricca L, Di Mario C, Bui L, Perniola S, Attar M, Gessi M, Fedele AL, Chilaka S, Somma D, Sansom SN, Filer A, McSharry C, Millar NL, Kirschner K, Nerviani A, Lewis MJ, Pitzalis C, Clark AR, Ferraccioli G, Udalova I, Buckley CD, Gremese E, McInnes IB, Otto TD, Kurowska-Stolarska M (2020) Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med 26(8):1295\u20131306","journal-title":"Nat Med"},{"issue":"2","key":"5_CR34","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2017-000527","volume":"3","author":"M Kurowska-Stolarska","year":"2017","unstructured":"Kurowska-Stolarska M, Alivernini S (2017) Synovial tissue macrophages: friend or foe? RMD Open 3(2):e000527","journal-title":"RMD Open"},{"issue":"3","key":"5_CR35","first-page":"620","volume":"34","author":"B Bresnihan","year":"2007","unstructured":"Bresnihan B, Gerlag DM, Rooney T, Smeets TJ, Wijbrandts CA, Boyle D, Fitzgerald O, Kirkham BW, McInnes IB, Smith M, Ulfgren AK, Veale DJ, Tak PP (2007) Synovial macrophages as a biomarker of response to therapeutic intervention in rheumatoid arthritis: standardization and consistency across centers. J Rheumatol 34(3):620\u2013622","journal-title":"J Rheumatol"},{"issue":"8","key":"5_CR36","doi-asserted-by":"publisher","first-page":"1860","DOI":"10.3390\/cells10081860","volume":"10","author":"L Roszkowski","year":"2021","unstructured":"Roszkowski L, Ciechomska M (2021) Tuning monocytes and macrophages for personalized therapy and diagnostic challenge in rheumatoid arthritis. Cells 10(8):1860","journal-title":"Cells"},{"issue":"10","key":"5_CR37","doi-asserted-by":"publisher","first-page":"2578","DOI":"10.1002\/art.10545","volume":"46","author":"N Kawanaka","year":"2002","unstructured":"Kawanaka N, Yamamura M, Aita T, Morita Y, Okamoto A, Kawashima M, Iwahashi M, Ueno A, Ohmoto Y, Makino H (2002) CD14+,CD16+ blood monocytes and joint inflammation in rheumatoid arthritis. Arthritis Rheum 46(10):2578\u20132586","journal-title":"Arthritis Rheum"},{"key":"5_CR38","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.intimp.2018.10.016","volume":"65","author":"AK Rana","year":"2018","unstructured":"Rana AK, Li Y, Dang Q, Yang F (2018) Monocytes in rheumatoid arthritis: Circulating precursors of macrophages and osteoclasts and, their heterogeneity and plasticity role in RA pathogenesis. Int Immunopharmacol 65:348\u2013359","journal-title":"Int Immunopharmacol"},{"issue":"2","key":"5_CR39","first-page":"89","volume":"61","author":"R Yano","year":"2007","unstructured":"Yano R, Yamamura M, Sunahori K, Takasugi K, Yamana J, Kawashima M, Makino H (2007) Recruitment of CD16+ monocytes into synovial tissues is mediated by fractalkine and CX3CR1 in rheumatoid arthritis patients. Acta Med Okayama 61(2):89\u201398","journal-title":"Acta Med Okayama"}],"container-title":["Methods in Molecular Biology","Autoimmunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/978-1-0716-4997-8_5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T11:04:10Z","timestamp":1781262250000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/978-1-0716-4997-8_5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,22]]},"ISBN":["9781071649961","9781071649978"],"references-count":39,"URL":"https:\/\/doi.org\/10.1007\/978-1-0716-4997-8_5","relation":{},"ISSN":["1064-3745","1940-6029"],"issn-type":[{"value":"1064-3745","type":"print"},{"value":"1940-6029","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,22]]},"assertion":[{"value":"22 November 2025","order":1,"name":"first_online","label":"First Online","group":{"name":"ChapterHistory","label":"Chapter History"}}]}}